Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,350 Δ1 bp 8.5% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3500A. 8.5% 462.8 / 34.0 153intergenic (+27/+463)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (76/63);  new base . (7/6);  total (84/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 155 total aligned reads displayed.

GATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAAT  >  NC_000913/3183201‑3183499
                                                                                                                                                      |                                                                                                                                                     
 atgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                                                                      >  1:630125/1‑150 (MQ=255)
  tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGCATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATg                                                                                                                                                     >  2:926743/1‑150 (MQ=255)
   taaTTAATGCCGCTCGCCAAAGAGAGTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                                                                                    <  2:619008/149‑1 (MQ=255)
    aaTTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGa                                                                                                                                                   >  2:1044617/1‑150 (MQ=255)
      ttAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTTGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGTAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACa                                                                                                                                                 <  2:1057012/150‑1 (MQ=255)
       tAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACaa                                                                                                                                                >  2:426476/1‑150 (MQ=255)
        aaTGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAc                                                                                                                                               <  1:1575521/150‑1 (MQ=255)
        aaTGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAc                                                                                                                                               <  1:1374144/150‑1 (MQ=255)
        aaTGCCGCTCGCCAAAGAGATTAACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAAc                                                                                                                                               >  1:207800/1‑150 (MQ=255)
         aTGCCGCTCGCCTAAGAGATTGACCCTAATACTATCCCCAGCTATGGCGGACTATTTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACt                                                                                                                                              <  1:844804/150‑1 (MQ=255)
            ccGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGCTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCt                                                                                                                                           <  1:386665/150‑1 (MQ=255)
                 cGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGTAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAg                                                                                                                                      <  2:466530/150‑1 (MQ=255)
                 cGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAg                                                                                                                                      >  2:505053/1‑150 (MQ=255)
                    cAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTTGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCtt                                                                                                                                   >  2:126186/1‑150 (MQ=255)
                     aaaGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTc                                                                                                                                  >  2:831916/1‑150 (MQ=255)
                       agagATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAATATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                                <  1:1023200/150‑1 (MQ=255)
                       agagATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                                >  2:112377/1‑150 (MQ=255)
                       agagATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                                <  2:630125/150‑1 (MQ=255)
                       agagATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                                <  1:1485774/150‑1 (MQ=255)
                        gagaGTGAGCCTAATCATACCCCCTGCTATGGCGTACTATGTGCAATGTCAGTGATTGGATGCAGTTTCGGGCTGCTACAAACGGCGGGAATTGGTTAAAACCAACATCAGCAGGAGTCCAGCGCAGCGACAACTTCTGCCAGCTTCACg                                                                                                                               <  2:254317/150‑1 (MQ=255)
                             tGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGc                                                                                                                          >  2:101182/1‑150 (MQ=255)
                              gACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATCGCATGGACACCTTCTGCCAGCTTCACGAAAGcc                                                                                                                         >  2:289880/1‑150 (MQ=255)
                                cccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                                                                       >  2:255307/1‑150 (MQ=255)
                                accTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                                                                       <  2:1468192/149‑1 (MQ=255)
                                   taataataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                                                                                    >  1:256029/1‑150 (MQ=255)
                                    aataataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                                                                   >  1:1467364/1‑150 (MQ=255)
                                     ataataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCATTATTCt                                                                                                                  >  2:1339435/1‑150 (MQ=255)
                                      taataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGACTTATTCTa                                                                                                                 >  2:1147878/1‑150 (MQ=255)
                                          aaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATAATACCtt                                                                                                             >  2:678680/1‑150 (MQ=255)
                                             cccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTc                                                                                                          >  2:1558721/1‑150 (MQ=255)
                                             cccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTc                                                                                                          >  1:785301/1‑150 (MQ=255)
                                              ccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTAGAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTCTCCAGCGCCTGGACAACTTCTGCCCGCTTCACGAAAGCCTTATTCTACCGTTTCg                                                                                                         >  1:1107084/1‑150 (MQ=255)
                                              ccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                                                                         >  1:889385/1‑150 (MQ=255)
                                              ccACCTATTGCCTACAATGTGCAATGTCAGAGATGGTAGTCAGTTTAGTTCTGCTACAAACGGCCGGAATTGGTTAAAAACAACCTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                                                                         <  2:82798/150‑1 (MQ=255)
                                               cAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCgg                                                                                                        <  2:882508/150‑1 (MQ=255)
                                                aGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCATCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                                                                       <  1:1176477/150‑1 (MQ=255)
                                                 gCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCCGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                                                                                      >  1:1597197/1‑150 (MQ=255)
                                                   tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                                                                                    <  1:1346353/150‑1 (MQ=255)
                                                      ggCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctc                                                                                                 <  1:1371506/150‑1 (MQ=255)
                                                      ggCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctc                                                                                                 >  2:687109/1‑150 (MQ=255)
                                                       gCGTACTATGTGGAATGTCAGTTATGTTATTCAGGTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGgctct                                                                                                <  2:213301/150‑1 (MQ=255)
                                                        cGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctctt                                                                                               >  2:766434/1‑150 (MQ=255)
                                                            cTATGTCGAATGTCAGTGATGGGAATCAGGTAAGTGCTGCTACACACCGCGGGAAATGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctct                                                                                           <  2:327259/150‑1 (MQ=255)
                                                                gtgGAATGTCAGTGATTCTATTCATTTTAGTGCTGATACAAACGTCGGGAATTTGTTAAAAACAACATAAGAAGTTGTCAATCGCATGGACAACTTCTTCCAGCTTCACGAAAGCCTTATTCTACCTTTTAGGCTTTCTCTTCTCTCGTa                                                                                       >  2:193107/1‑150 (MQ=255)
                                                                gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTa                                                                                       >  2:718040/1‑150 (MQ=255)
                                                                 tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTAc                                                                                      <  1:408268/150‑1 (MQ=255)
                                                                 tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTCCCAGCTTCACGAAAGCCTTATTCTACCTTTTAGTCTGTCTCTTCTCTCGTAc                                                                                      >  1:1157763/1‑150 (MQ=255)
                                                                   gAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                                                                    <  2:1690113/150‑1 (MQ=255)
                                                                     aTGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                                                                                  <  2:1281100/150‑1 (MQ=255)
                                                                      tGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                                                                                 <  1:1270752/150‑1 (MQ=255)
                                                                       gTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTa                                                                                <  1:335339/150‑1 (MQ=255)
                                                                         cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                                                                              <  1:101182/150‑1 (MQ=255)
                                                                           gTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTa                                                                            <  1:650242/150‑1 (MQ=255)
                                                                               tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCCGCGCATGGACAACTTCTGCCAGCTTCACGAAGCCTTATTCTACCTTTTAGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                                                                       >  2:308668/1‑150 (MQ=255)
                                                                               tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                                                                        >  2:963554/1‑150 (MQ=255)
                                                                                gggATTCAGTTTAGTGCTGCTACAAACGTCGGGAATTGGTTAAAAACAACATCAGAAGGTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                                                                       <  1:1362420/150‑1 (MQ=255)
                                                                                 ggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGtt                                                                      >  2:242907/1‑150 (MQ=255)
                                                                                  gATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc                                                                     <  1:1339435/150‑1 (MQ=255)
                                                                                   aTTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                                                                    <  1:351580/150‑1 (MQ=255)
                                                                                    ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                                                                   <  2:1725244/150‑1 (MQ=255)
                                                                                      cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTACAGCGCATGGACAACTTCTCCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCAGTCTCTTCTCACGTACTGTTTAAGAATTAGTTCcgcg                                                                 >  1:468076/1‑150 (MQ=255)
                                                                                              ggctgcgACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGGCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc                                                         <  1:1109756/149‑1 (MQ=255)
                                                                                                  gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTg                                                     <  1:1046423/150‑1 (MQ=255)
                                                                                                   ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGc                                                    >  1:835998/1‑150 (MQ=255)
                                                                                                    tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCg                                                   >  2:711723/1‑150 (MQ=255)
                                                                                                      cAAACGGCGGGAATTGGTTAAAAACATCATCTGAAGTTTTCCAGCGCATGGACAACTTCTGCCAGCTTCTCGAAAGCCTTATTCTACCTTATCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtc                                                 <  1:255307/150‑1 (MQ=255)
                                                                                                      cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTtctctc                                                                                          <  2:2431/109‑1 (MQ=255)
                                                                                                      cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTtctctc                                                                                          >  1:2431/1‑109 (MQ=255)
                                                                                                          cggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTg                                             <  2:503123/150‑1 (MQ=255)
                                                                                                           ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTGATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTATGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGt                                            >  1:912346/1‑150 (MQ=255)
                                                                                                                 aaTTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGc                                      <  2:1601783/150‑1 (MQ=255)
                                                                                                                  aTTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCg                                     <  1:17669/150‑1 (MQ=255)
                                                                                                                     ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGaaa                                  >  2:1692499/1‑150 (MQ=255)
                                                                                                                     ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAACTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGaaa                                  >  2:1143363/1‑150 (MQ=255)
                                                                                                                      tttAAAAACAACATAAGAAGTTGTCCAGAGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAACTTTTAGGCTGTCTCTTCTGTCGTACTGTTTAAGTATTTGTTCCGAGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAAc                                 >  1:1247060/2‑150 (MQ=255)
                                                                                                                       ttAAATACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAACCg                                >  2:471829/1‑150 (MQ=255)
                                                                                                                       ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACg                                <  1:1044617/150‑1 (MQ=255)
                                                                                                                       ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCCGGCGAAACg                                <  1:831916/150‑1 (MQ=255)
                                                                                                                         aaaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGcc                              <  2:129646/150‑1 (MQ=255)
                                                                                                                           aaaCAACATCCGAAGTTGTCCATCGCATTGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCg                            <  1:318138/150‑1 (MQ=255)
                                                                                                                               aacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACaa                        <  2:780885/150‑1 (MQ=255)
                                                                                                                               aacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATCCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACaa                        >  2:623764/1‑150 (MQ=255)
                                                                                                                                 caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTGAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATa                      >  1:523287/1‑150 (MQ=255)
                                                                                                                                    cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTACGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGATTCGCCCGACAATAACg                   >  1:758584/1‑150 (MQ=255)
                                                                                                                                     aGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAACCGCCCGACAATAACGt                  >  1:964446/1‑150 (MQ=255)
                                                                                                                                     aGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGt                  >  2:1655257/1‑150 (MQ=255)
                                                                                                                                      gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGtt                 >  1:1386514/1‑150 (MQ=255)
                                                                                                                                       aaGTTGTCCATGCGCTGGACAGCTTGTGCCAGCTTCAGGTAAGCCTTTTTCTACCTTTTCGGCTGTCTCTTCTCTCGAACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGttt                >  2:1451103/1‑150 (MQ=255)
                                                                                                                                       aaGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGttt                >  2:1371889/1‑150 (MQ=255)
                                                                                                                                        aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTc               >  2:1401196/1‑150 (MQ=255)
                                                                                                                                            gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAAt           >  1:590066/1‑150 (MQ=255)
                                                                                                                                            gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAAt           <  1:1558721/150‑1 (MQ=255)
                                                                                                                                              ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATgg         >  2:453280/1‑149 (MQ=255)
                                                                                                                                              ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGc         <  1:197965/150‑1 (MQ=255)
                                                                                                                                                  gcgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAgg      <  2:1194812/150‑1 (MQ=255)
                                                                                                                                                   cgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGa     <  2:1507455/150‑1 (MQ=255)
                                                                                                                                                   cgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGa     <  1:47781/150‑1 (MQ=255)
                                                                                                                                                   cgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGa     <  2:1234320/150‑1 (MQ=255)
                                                                                                                                                    gcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTTCTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGACTCTGTCCTCGCGCAACGCCCGACAATAACGTGTCAAATGCAGGaa    >  2:1192598/1‑150 (MQ=255)
                                                                                                                                                      aTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCCAGCAGTGCGTCTCTGTCCAGGCGAAACGGCCGACAATAACGTTTCAAATGCAGGAAAt  >  1:1241064/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAAT  >  NC_000913/3183201‑3183499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: