Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,120 Δ1 bp 6.0% coding (186/621 nt) fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1200G. 6.0% 360.9 / 17.3 116coding (186/621 nt)fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base G (57/51);  new base . (2/5);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 117 total aligned reads displayed.

GCTACGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGG  >  NC_000913/4428975‑4429268
                                                                                                                                                  |                                                                                                                                                     
gcTACGGCATTGGTTTGCAGGTAGGGCAACAACTGCGGGAATCTGGCCTGGAAGGTCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTg                                                                                                                                                   <  1:1330916/150‑1 (MQ=255)
  tACGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGaa                                                                                                                                                 >  2:496225/1‑150 (MQ=255)
    tgccATTGGTTTGCAGGTAGGGCAACAAGTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCATAAGCACAGGTTGCAGGTATTGCCGATGCGCTGGAAGGCTAACATCCGGCTGTTCCGGATGATGTGGTGCAGCGCGCGCTGCGTGAAAt                                                                                                                                               <  1:825544/147‑1 (MQ=255)
     ggCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATc                                                                                                                                              >  1:872029/1‑150 (MQ=255)
           ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                       >  1:939050/1‑150 (MQ=255)
           ggTGTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCGGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                       <  1:976236/150‑1 (MQ=255)
                  aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcgcgcga                                                                                                                                <  1:735538/150‑3 (MQ=255)
                  aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcgcg                                                                                                                                 <  2:907940/150‑2 (MQ=255)
                    gTAGGGCAACAACTGTGTGAATCTGGCCTGTACGGGCTGCTGCCAGAATCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                              <  1:1131021/150‑1 (MQ=255)
                    gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                              <  1:625323/150‑1 (MQ=255)
                     gaGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCTGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATg                                                                                                                             <  2:147649/149‑1 (MQ=255)
                      aGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGc                                                                                                                            <  1:210951/150‑1 (MQ=38)
                       gggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGCTGcc                                                                                                                           >  1:196483/1‑150 (MQ=255)
                        ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCg                                                                                                                          >  1:1238832/1‑150 (MQ=255)
                           aacaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGcaccac                                                                                                                       <  2:1698508/150‑6 (MQ=37)
                           aacaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGcaccac                                                                                                                       <  2:1691953/150‑6 (MQ=25)
                            acaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcg                                                                                                                      >  2:789893/1‑150 (MQ=255)
                            acaacTGAGGGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTGCCGGTTGATGTGGTGCATCGCGCGCTTCGTGAAATCCACGAGCGCGCCGATGCCGTtcg                                                                                                                      <  1:1274358/150‑1 (MQ=255)
                              aacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTGGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtc                                                                                                                    <  1:1357971/150‑1 (MQ=255)
                               acTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                   <  1:490072/150‑1 (MQ=255)
                               acTGAGTGAAGCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                   <  2:1483902/150‑1 (MQ=255)
                                 tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtc                                                                                                                 >  1:426520/1‑150 (MQ=255)
                                 tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtc                                                                                                                 >  1:183299/1‑150 (MQ=255)
                                  gAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtcc                                                                                                                >  1:279445/1‑149 (MQ=255)
                                   aGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAg                                                                                                               <  1:1250793/150‑1 (MQ=255)
                                    gTGAATCTGGCCTGGAAGTGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGc                                                                                                              >  2:215912/1‑150 (MQ=255)
                                     tGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCg                                                                                                             <  2:685726/150‑1 (MQ=255)
                                        aTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGttt                                                                                                          >  2:892125/1‑150 (MQ=255)
                                          cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                        >  2:246885/1‑150 (MQ=255)
                                          cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                        <  2:553527/150‑1 (MQ=255)
                                             gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGGTCGTCGTCAGCGTTTCCAgg                                                                                                     >  1:167621/1‑150 (MQ=255)
                                               cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                   >  2:116619/1‑150 (MQ=255)
                                                  gAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGAGGCGCTGGAAGGCAAACATCCGGCAGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGTAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATg                                                                                                <  1:1503727/150‑1 (MQ=255)
                                                    aGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                              >  1:1276797/1‑150 (MQ=255)
                                                      ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAATGCAAACATACGGCTGTACCCGTTGATGAGGTGCATCCCGCCCTGCGTGAAATCCACGAGCGCGCCGATCCCGTACTTCGTCAGCGTGTCCAGGCGATGgcag                                                                                            >  2:672493/1‑148 (MQ=255)
                                                      ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                            >  1:61162/1‑150 (MQ=255)
                                                       gctgctGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgc                                                                                           <  1:1726774/150‑1 (MQ=255)
                                                        ctgctgCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGATTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgct                                                                                          <  2:387379/150‑1 (MQ=255)
                                                          gctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGa                                                                                        >  1:1731038/1‑150 (MQ=255)
                                                          gctgcCAGAAGCACTGGTTGCAGGTAGTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGa                                                                                        <  1:1156923/150‑1 (MQ=255)
                                                           ctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGaa                                                                                       >  1:521344/1‑150 (MQ=255)
                                                             gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                     >  2:899083/1‑150 (MQ=255)
                                                             gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                     <  2:613703/150‑1 (MQ=255)
                                                               cAGAAGCTCTGGTGGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                   <  2:481774/150‑1 (MQ=255)
                                                               cAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCTCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                   >  2:1113529/1‑150 (MQ=255)
                                                                   aGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGaa                                                                               <  2:100690/150‑1 (MQ=255)
                                                                    gCACTGGTTGCAGGTTTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGaaa                                                                              <  1:357602/150‑1 (MQ=255)
                                                                     cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGAGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                             <  2:279445/150‑1 (MQ=255)
                                                                      aCTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCAATGGCTGCTGAAGGTGTGAAATa                                                                            >  1:370623/1‑150 (MQ=255)
                                                                         ggTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCt                                                                         >  1:1243405/1‑150 (MQ=255)
                                                                          gTTGCAGGTATTGCCGATGCGCTGGCAGGCAAACATCCGGCTGTTCCGGTGGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                        <  1:266429/150‑1 (MQ=255)
                                                                           ttGCAGGTATTGCCGTTGCGCTGGAAGGCATACATCCGGCTGTGCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTgg                                                                       <  2:939050/150‑1 (MQ=255)
                                                                              cAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                    >  1:1025160/1‑150 (MQ=255)
                                                                                ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                     >  1:1251258/1‑115 (MQ=255)
                                                                                ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                     <  2:1251258/115‑1 (MQ=255)
                                                                                ggTAGTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaagaa                                                                  <  1:56367/150‑1 (MQ=255)
                                                                                    ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACg                                                              <  1:1590548/150‑1 (MQ=255)
                                                                                      gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                            >  1:1641175/1‑150 (MQ=255)
                                                                                      gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                            >  1:1664486/1‑150 (MQ=255)
                                                                                         gATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaa                                                         >  2:1195070/1‑150 (MQ=255)
                                                                                           tGCGCTGGAATGCAAACATCCGGCTGTTCCGGTTGATGTTGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaaaa                                                       <  2:387590/150‑1 (MQ=255)
                                                                                             cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                     >  1:24778/1‑150 (MQ=255)
                                                                                              gcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaaga                                                    >  2:748435/1‑150 (MQ=255)
                                                                                                tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                  <  1:246885/150‑1 (MQ=255)
                                                                                                tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                  <  2:1042164/150‑1 (MQ=255)
                                                                                                 ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                 <  1:708023/150‑1 (MQ=255)
                                                                                                     ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                             <  2:872029/150‑1 (MQ=255)
                                                                                                     ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                             >  1:1673940/1‑150 (MQ=255)
                                                                                                     ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATCCCGTTCGTCGTCAGCGTTTCCATGCGATGGCTGCTGAAGGGGTGAACTACCTCCAAGCAAACCCCAACCACGCAGGtgtg                                             >  1:301213/1‑150 (MQ=255)
                                                                                                       cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                           <  1:1686936/150‑1 (MQ=255)
                                                                                                       cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                           >  2:427288/1‑150 (MQ=255)
                                                                                                        aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAAt                                          >  1:618551/1‑150 (MQ=255)
                                                                                                          aCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAg                                        >  1:427764/1‑150 (MQ=255)
                                                                                                               cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGTTGGCTGCTGAAGGTGTGAATTACCGGGAAGAAAACGCCAAAACAGAAGGTGGGCTTAGCCCCg                                   >  1:1136541/1‑150 (MQ=255)
                                                                                                               cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCg                                   <  1:1113529/150‑1 (MQ=255)
                                                                                                                ggaacagccGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                  >  2:218932/8‑150 (MQ=255)
                                                                                                                ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                  >  1:1129311/1‑150 (MQ=255)
                                                                                                                    gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCTCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATCGCACCGAATCt                              >  2:1639460/1‑150 (MQ=255)
                                                                                                                    gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCt                              <  1:1483105/150‑1 (MQ=255)
                                                                                                                    gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCt                              <  1:1347366/150‑1 (MQ=255)
                                                                                                                     gtCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCAGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                             >  1:632118/2‑150 (MQ=255)
                                                                                                                        cGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCCCGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGAGGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                          <  2:130917/150‑1 (MQ=255)
                                                                                                                          gTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGGCAGCGTTTCCAGGCGAGGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTg                        <  1:915435/150‑1 (MQ=255)
                                                                                                                           ttGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGc                       <  1:806836/150‑1 (MQ=255)
                                                                                                                            tGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGCGCGCGGCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCa                      >  1:695063/1‑150 (MQ=255)
                                                                                                                             gATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCaa                     >  2:486667/1‑150 (MQ=255)
                                                                                                                                  ggtgCATCGCGCGCTGAGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCATAAAAGAAGGTGTTAATAGCACCGATTCTGGCCTGCAATTCcg                <  1:368679/150‑1 (MQ=255)
                                                                                                                                   gtgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgc               >  2:1239503/1‑150 (MQ=255)
                                                                                                                                     gCATCGCGCGCTGCGTGAAAACCACGAGCGCGCCGATGCCGTTCGGCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGAGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt             <  1:599487/150‑1 (MQ=255)
                                                                                                                                      catcggcgcgcTCGTGGATTTCACGCAGCGCGCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg            >  1:246269/6‑150 (MQ=38)
                                                                                                                                       atcggcgcgcTCGTGGATTTCACGCAGCGCGAAGATGCCGTTCGTCGTCATAGTTTCCAGGCGATGGCTGCTGAAGGTGTGAATTACCTGGAAGAAAACGACAAAATAGAAGGTGTGAATATCAACGAATCTGTCCTGCAATTCCTCGTg            >  1:954250/5‑150 (MQ=255)
                                                                                                                                       aTCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa           <  2:521344/150‑1 (MQ=255)
                                                                                                                                       aTCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa           >  2:326447/1‑150 (MQ=255)
                                                                                                                                        tCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt          <  2:773501/150‑1 (MQ=255)
                                                                                                                                        gcgcgcgCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt          <  2:719151/149‑1 (MQ=255)
                                                                                                                                           gcgcgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCa        >  2:421023/1‑150 (MQ=255)
                                                                                                                                              cgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAcc     <  1:700409/150‑1 (MQ=255)
                                                                                                                                               gcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCa    >  1:1454549/1‑150 (MQ=255)
                                                                                                                                                cTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg   <  2:1731038/150‑1 (MQ=255)
                                                                                                                                                 ggCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAgg  <  1:215912/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCTACGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGG  >  NC_000913/4428975‑4429268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: