Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,299,273 Δ1 bp 5.7% intergenic (+28/‑1909) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,299,2730T. 5.7% 394.5 / 15.2 123intergenic (+28/‑1909)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (68/48);  new base . (5/2);  total (73/50)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

Only 100 of 123 total aligned reads displayed.

GTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACA  >  NC_000913/1299126‑1299421
                                                                                                                                                    |                                                                                                                                                    
gTTTTACGCCGGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGc                                                                                                                                                    >  1:66161/1‑150 (MQ=255)
gTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTTATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGc                                                                                                                                                    >  2:1548020/1‑150 (MQ=255)
  tttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTc                                                                                                                                                  <  1:161261/150‑1 (MQ=255)
     aCGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGt                                                                                                                                               <  2:789155/150‑1 (MQ=255)
      cGCCATACCGGCATCAAAGTGATTACGCGTATTATGGGGCTATTGCTGATGGAATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGtt                                                                                                                                              <  1:205985/150‑1 (MQ=255)
      cGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGATGCCATGCTCCGtt                                                                                                                                              >  1:131427/1‑150 (MQ=255)
       gCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGttt                                                                                                                                             >  1:1627742/1‑150 (MQ=255)
       gCCAGACCGGCATAATCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCTTTGGGTATTGAATTTATCGTTACTGATATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGttt                                                                                                                                             <  2:1549693/150‑1 (MQ=255)
        ccAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTa                                                                                                                                            <  2:665995/150‑1 (MQ=255)
         cAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtttca                                                                                                                                           <  1:1348532/150‑3 (MQ=255)
             ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTTCTTCg                                                                                                                                       >  1:780792/1‑150 (MQ=255)
             ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCg                                                                                                                                       <  1:446023/150‑1 (MQ=255)
                  aTCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCatt                                                                                                                                  >  1:564435/1‑150 (MQ=255)
                    cAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattat                                                                                                                                >  2:1612563/1‑150 (MQ=255)
                    cAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattat                                                                                                                                >  1:996482/1‑150 (MQ=255)
                     aaCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattatt                                                                                                                               >  1:553283/1‑150 (MQ=255)
                      aCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTAttt                                                                                                                              >  1:1180762/1‑150 (MQ=255)
                       cGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTAtttt                                                                                                                             <  2:136883/150‑1 (MQ=255)
                         tGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTa                                                                                                                           >  2:1426047/1‑150 (MQ=255)
                                gcttattatGTGGATATTTATGATGGAATTGGGGATTGAATTTATAGTTACTGGTATTAAGGGGATTTTCACCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGtt                                                                                                                    <  2:1142682/147‑1 (MQ=255)
                                 cgTATTATTGGGCTATTGCTGATGGCATTGGTGATTGAATTTCTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTAAATTAATTCCTTTCAAATGAAACTGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTc                                                                                                                   <  1:266802/150‑1 (MQ=255)
                                 cgTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTc                                                                                                                   >  2:1562629/1‑150 (MQ=255)
                                  gTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTcc                                                                                                                  >  1:1377522/1‑150 (MQ=255)
                                       atGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTTTTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCgcgc                                                                                                             <  2:1594916/150‑1 (MQ=255)
                                        tGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCa                                                                                                            >  2:1475346/1‑150 (MQ=255)
                                        tGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCa                                                                                                            >  2:476809/1‑150 (MQ=255)
                                         ggggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAg                                                                                                           <  2:39421/150‑1 (MQ=255)
                                          gggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGt                                                                                                          >  2:401837/1‑150 (MQ=255)
                                          gggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGt                                                                                                          >  1:832564/1‑150 (MQ=255)
                                               aTTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATca                                                                                                     <  2:553283/150‑1 (MQ=255)
                                                   cTGATGGCATTGTGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaag                                                                                                 <  1:376856/150‑1 (MQ=255)
                                                       tGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaa                                                                                             <  1:945472/150‑1 (MQ=255)
                                                       tGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaa                                                                                             <  2:1428110/150‑1 (MQ=255)
                                                              ggggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATa                                                                                      <  1:48371/150‑1 (MQ=255)
                                                                 gATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGt                                                                                   >  2:500627/1‑150 (MQ=255)
                                                                  aTTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTa                                                                                  <  1:1134859/150‑1 (MQ=255)
                                                                     gAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTTCTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTaaaa                                                                               >  1:330255/1‑150 (MQ=255)
                                                                     gAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTaaaa                                                                               >  1:958281/1‑150 (MQ=255)
                                                                       aTTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAat                                                                             <  1:1612563/150‑1 (MQ=255)
                                                                          tATCGTTACTGGTATTAAGGTGATTTTCCCCGGACTGCTTATTTAATTCCTTTCTAATGAAACGGAGCTGCCATTCTCCGTTTACTTCTTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAACGGAATAGGTAAAAATAtt                                                                          <  1:46169/150‑1 (MQ=255)
                                                                           aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTTTCAAAAGCAAAAGGAATAGGTAAAAATAttc                                                                         >  2:841287/1‑150 (MQ=255)
                                                                           aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaaag                                                                                           >  2:20536/1‑132 (MQ=255)
                                                                             cGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTAGTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttctt                                                                       >  2:276734/1‑150 (MQ=255)
                                                                              gTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttcttc                                                                      >  2:559282/1‑150 (MQ=255)
                                                                                tACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTtctc                                                                    >  2:67721/1‑150 (MQ=255)
                                                                                 aCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCa                                                                   <  2:1627742/150‑1 (MQ=255)
                                                                                 aCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCa                                                                   <  2:677066/150‑1 (MQ=255)
                                                                                    ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                                >  2:1206721/1‑150 (MQ=255)
                                                                                    ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                                >  1:537711/1‑150 (MQ=255)
                                                                                    ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                                >  1:504570/1‑150 (MQ=255)
                                                                                         tAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACag                                                           <  2:1507064/150‑1 (MQ=255)
                                                                                          aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACTGAGGTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAACAGCTAAAGGAATAGGTTAAAATATTCTTCTCAAATTActct                                                          >  2:265165/1‑147 (MQ=255)
                                                                                          aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagt                                                          >  2:1244116/1‑150 (MQ=255)
                                                                                          aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagt                                                          >  2:1495846/1‑150 (MQ=255)
                                                                                          aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGAGAAGTTATAAAAAACAAAAGAAATAGTTAAATAAATTATTATAAAATTACatt                                                          >  1:293307/1‑148 (MQ=255)
                                                                                           aGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtt                                                         <  2:904353/150‑1 (MQ=255)
                                                                                            ggggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtta                                                        >  1:988647/1‑150 (MQ=255)
                                                                                            ggggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtta                                                        >  1:627809/1‑150 (MQ=255)
                                                                                            ggggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtta                                                        >  2:738381/1‑150 (MQ=255)
                                                                                             gggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTGATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttat                                                       >  1:122586/1‑149 (MQ=255)
                                                                                               gATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttagtt                                                     >  1:307354/1‑150 (MQ=255)
                                                                                               gATTTTCCCCGGCCTGCTTAATTAAATCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCAATATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAAAAGGTAAAAATATTCTTCTCAAATTACagttagtt                                                     >  2:315072/1‑150 (MQ=255)
                                                                                                aTTTTCCCCGGCCTGCTTAATTACTTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAgttagtta                                                    <  2:183671/150‑1 (MQ=255)
                                                                                                aTTTTCACCGGCATGATTAATTAATTCCTTTCATATGATACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAGGTAATAGGTAAAAATATTCTTCTCAAATTACAgttagtta                                                    <  2:133705/150‑1 (MQ=255)
                                                                                                  tttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAAAGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTtata                                                  <  1:1051657/150‑1 (MQ=255)
                                                                                                   ttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATaa                                                 >  2:163015/1‑150 (MQ=255)
                                                                                                    tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                                <  1:1475346/150‑1 (MQ=255)
                                                                                                    tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                                <  1:466629/150‑1 (MQ=255)
                                                                                                    tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                                >  1:870502/1‑150 (MQ=255)
                                                                                                    tCCCCGGCCTGCTTAATTAATTACTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                                <  2:1172116/150‑1 (MQ=255)
                                                                                                     ccccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGAAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAgg                                               >  2:1692664/1‑150 (MQ=255)
                                                                                                      cccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGa                                              >  1:488461/1‑150 (MQ=255)
                                                                                                            cTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGAAATCCt                                        <  1:1089080/150‑1 (MQ=255)
                                                                                                                  aattaattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTg                                  <  1:1548020/150‑1 (MQ=255)
                                                                                                                  aattaattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTg                                  <  2:1099811/150‑1 (MQ=255)
                                                                                                                     taattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCtt                               >  1:610221/1‑150 (MQ=255)
                                                                                                                       attCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtct                             >  2:860524/1‑150 (MQ=255)
                                                                                                                          ccTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAACTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcct                          >  1:338962/1‑150 (MQ=255)
                                                                                                                          ccTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcct                          <  2:1355260/150‑1 (MQ=255)
                                                                                                                            tttCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCa                        <  1:163015/150‑1 (MQ=255)
                                                                                                                               cAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACca                     >  2:383301/1‑150 (MQ=255)
                                                                                                                               cAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACca                     <  1:100763/150‑1 (MQ=255)
                                                                                                                                aaaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcat                    >  1:811545/1‑150 (MQ=255)
                                                                                                                                aaaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcat                    >  1:588062/1‑150 (MQ=255)
                                                                                                                                 aaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatc                   >  1:596552/1‑150 (MQ=255)
                                                                                                                                  aTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca                  >  1:357027/1‑150 (MQ=255)
                                                                                                                                    gAAACGGAGCTGCCATTCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg                <  2:771572/150‑1 (MQ=255)
                                                                                                                                    gAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg                >  2:740423/1‑150 (MQ=255)
                                                                                                                                    gAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCATCACCATCATg                >  1:206540/1‑150 (MQ=255)
                                                                                                                                     aaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt               >  2:907233/1‑150 (MQ=255)
                                                                                                                                     aaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt               >  2:622685/1‑150 (MQ=255)
                                                                                                                                     aaaCGGAGCATGGCAGCTCCGTTTACTTCCTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTATTCTCAAATTACAGTTAGTTATAAGGATATCCTTAAATGCTTATCCTCACCATCATGt               >  1:66151/1‑150 (MQ=255)
                                                                                                                                      aaCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGtt              >  2:1389684/1‑150 (MQ=255)
                                                                                                                                            gCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTAtttta        >  1:1449029/1‑149 (MQ=255)
                                                                                                                                            gCTGCCATGCTCAGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGTAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTc        <  1:1448451/150‑1 (MQ=255)
                                                                                                                                             cTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCg       >  2:570963/1‑150 (MQ=255)
                                                                                                                                                gCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCtt                               <  1:153266/124‑1 (MQ=255)
                                                                                                                                                gCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCtt                               >  2:153266/1‑124 (MQ=255)
                                                                                                                                                  cATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCcac   >  1:590776/1‑150 (MQ=255)
                                                                                                                                                   aTGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCcaca  <  1:505718/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACA  >  NC_000913/1299126‑1299421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: