Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,524,463 Δ1 bp 5.2% intergenic (+95/+18) yncE → / ← ansP ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,524,4630A. 5.2% 347.1 / 11.8 115intergenic (+95/+18)yncE/ansPATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base A (50/58);  new base . (2/4);  total (53/62)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 115 total aligned reads displayed.

ACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCA  >  NC_000913/1524315‑1524602
                                                                                                                                                     |                                                                                                                                           
aCTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAg                                                                                                                                            >  1:1535752/1‑150 (MQ=255)
   aaaCAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCg                                                                                                                                         >  2:341440/1‑150 (MQ=255)
     aCAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                                                                       <  1:1014126/150‑1 (MQ=255)
       agcagGAAGCTACCCAGCCAGACGCTGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCa                                                                                                                                     <  1:469415/150‑1 (MQ=255)
        cccggAAGCTCCCCAGCCAGACGCGGTGAGTCGTCTTGCGCGGTACTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaa                                                                                                                                    <  2:300787/147‑1 (MQ=255)
          agGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaaat                                                                                                                                  <  1:1169805/150‑3 (MQ=255)
           ggAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCAAatt                                                                                                                                 <  1:677604/150‑1 (MQ=255)
              aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGc                                                                                                                              >  1:294332/1‑150 (MQ=255)
                cTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                                                                            <  1:369771/150‑1 (MQ=255)
                   cccAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGTGCAATTTTGCAATTa                                                                                                                         >  1:1236171/1‑150 (MQ=255)
                        ccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGGCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCg                                                                                                                    <  1:818358/150‑1 (MQ=255)
                          agACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGcc                                                                                                                  >  2:1455815/1‑150 (MQ=255)
                            ccGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGt                                                                                                                <  1:941301/149‑1 (MQ=255)
                              gATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGttt                                                                                                              >  1:548764/1‑150 (MQ=255)
                               aTGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGGGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc                                                                                                             <  1:485778/150‑1 (MQ=255)
                                 gtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAg                                                                                                           >  1:1492116/1‑150 (MQ=255)
                                    aTTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCt                                                                                                        <  2:1421457/150‑1 (MQ=255)
                                       cggaTTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaa                                                                                                     <  1:876474/147‑1 (MQ=255)
                                       cGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaa                                                                                                     >  2:1080507/1‑150 (MQ=255)
                                        gTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaa                                                                                                    >  1:998100/1‑150 (MQ=255)
                                         tATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAac                                                                                                   >  1:606679/1‑150 (MQ=255)
                                          gttGCGCTGTAATAGATATGGCGAGGGTGAAAAGGGTGAGCCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTGGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaccc                                                                                                  >  1:482794/2‑147 (MQ=255)
                                          aTTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAaca                                                                                                  >  1:482790/1‑150 (MQ=255)
                                            tGCGCTGTAATAGATATGGCGAGGGTTAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAAcaca                                                                                                <  1:1648564/150‑1 (MQ=255)
                                               gcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                                                             <  2:699436/150‑1 (MQ=255)
                                               gcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                                                             <  2:1411720/150‑1 (MQ=255)
                                                cTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGAAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAttt                                                                                            >  1:936330/1‑150 (MQ=255)
                                                 tGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTc                                                                                           <  1:1636609/150‑1 (MQ=255)
                                                  gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                                                          >  1:247568/1‑150 (MQ=255)
                                                  gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                                                          <  2:1359316/150‑1 (MQ=255)
                                                    aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCTTCAGTAATCTGAATTTTCCCGGCACGTAGACGGGCAATTTAGCAATTAACTCGCCGTTTCAGGCTTAAAAACAATTTCCtt                                                                                        >  2:1161966/1‑149 (MQ=255)
                                                    aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg                                                                                        >  1:638182/1‑150 (MQ=255)
                                                    aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg                                                                                        <  2:1185422/150‑1 (MQ=255)
                                                      tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtt                                                                                      <  2:960146/150‑1 (MQ=255)
                                                      tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtt                                                                                      <  1:1323290/150‑1 (MQ=255)
                                                       aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttt                                                                                     >  1:240952/1‑150 (MQ=255)
                                                       aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttt                                                                                     >  2:401922/1‑150 (MQ=255)
                                                        gATATGGGGAGGGTGAAAACGGTGAGACTTGCCGGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTGGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                                                                    <  2:482794/150‑1 (MQ=255)
                                                        gATATGGCGAGGGTGAAAACGGTGATACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCCGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                                                                    <  2:1228973/150‑1 (MQ=255)
                                                        gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                                                                    <  2:482790/150‑1 (MQ=255)
                                                         atctgccGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGTCGTATCCGGCACTTTAGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGTGCAATTTTGCAATTAACTCGCCCTTTCAGGCTTAAACACAATTTCCTGttttt                                                                                   <  1:111667/144‑1 (MQ=255)
                                                         atatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttttt                                                                                   <  2:550486/150‑1 (MQ=255)
                                                          tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttc                                                                                  >  2:1150021/1‑150 (MQ=255)
                                                           atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                                                                 >  2:1201173/1‑150 (MQ=255)
                                                             ggCGCGGGTGAAACCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                                                               <  2:1550823/150‑1 (MQ=255)
                                                             ggCGAGGGTGAAAACGGTTAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGGTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                                                               <  2:493107/150‑1 (MQ=255)
                                                             ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                                                               >  2:953521/1‑150 (MQ=255)
                                                              gcgctgctGAAAACGGTCAGACTAGCCTGCCTTTTCTCACGGCGTAGCCGGCCCTTGCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTGCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                                                              <  2:169112/143‑1 (MQ=255)
                                                              gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                                                              >  2:615376/1‑150 (MQ=255)
                                                                 aGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCt                                                                           <  1:563326/150‑1 (MQ=255)
                                                                  gggTGAAAACGGTGAGACTTTCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCATTAATCTGAATTTGCACGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCtt                                                                          <  2:1082251/150‑1 (MQ=255)
                                                                     tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt                                                                       >  1:1267144/1‑150 (MQ=255)
                                                                      gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTc                                                                      >  2:1508455/1‑150 (MQ=255)
                                                                      gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGACACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTc                                                                      <  1:1244025/150‑1 (MQ=255)
                                                                       aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                                                                     <  2:576134/150‑1 (MQ=255)
                                                                       aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCAGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                                                                     >  1:1500780/1‑150 (MQ=255)
                                                                         aaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGTCACTTTCGACAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATATTGCAATTAACTCGCCGTTTCAGGCTTAATCACAGTTTCCTGTTTTTCTTCATCTTCCTcgac                                                                   >  1:277602/1‑150 (MQ=255)
                                                                          aCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                                                                  <  2:669924/150‑1 (MQ=255)
                                                                          aCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                                                                  >  2:442343/1‑150 (MQ=255)
                                                                            ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCgacgac                                                                <  2:247568/150‑1 (MQ=255)
                                                                            ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCgacgac                                                                >  1:1419395/1‑150 (MQ=255)
                                                                             gTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                                                               >  2:866522/1‑150 (MQ=255)
                                                                             gTGAGACTTGCCTGCATTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                                                               >  2:872101/1‑150 (MQ=255)
                                                                              tGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                                                              <  2:1706648/150‑1 (MQ=255)
                                                                              tGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                                                              <  2:1680729/150‑1 (MQ=255)
                                                                                agaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc                                                            <  2:1514677/150‑1 (MQ=255)
                                                                                          gcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgt                                                  <  2:548764/150‑1 (MQ=255)
                                                                                          gcctTTTATCACGGCGTATCCGGCACTTTCGGCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgt                                                  <  2:1048924/150‑1 (MQ=255)
                                                                                           cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgtg                                                 <  2:1535752/150‑1 (MQ=255)
                                                                                            ctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAATAACTCGCCGTTTCAGGCTTAAACACAAATTCCTGTTTTTCTTCATAATCATCGACGACTGGCGCAGTGCTgaga                                                >  1:1044067/1‑150 (MQ=255)
                                                                                             ttttATCACGGCGGTTCCGGCACTTTCGTAAGTAAGCTGAATTTGCCCGGCACGTAGCCGTGAAATTTTTAAATTAACACGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGGGCTGTGaa                                               <  2:281754/150‑1 (MQ=255)
                                                                                                  tCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa                                          >  1:765508/1‑150 (MQ=255)
                                                                                                   cACGGCGTATCCGTCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg                                         <  1:1020241/150‑1 (MQ=255)
                                                                                                   cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg                                         >  1:898436/1‑150 (MQ=255)
                                                                                                    aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        <  2:1226925/150‑1 (MQ=255)
                                                                                                    aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        <  1:953521/150‑1 (MQ=255)
                                                                                                        cGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcg                                    <  1:1290925/150‑1 (MQ=255)
                                                                                                            tCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACTTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGtt                                <  1:640161/150‑1 (MQ=255)
                                                                                                              cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        >  1:1152278/1‑140 (MQ=255)
                                                                                                              cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        <  2:1152278/140‑1 (MQ=255)
                                                                                                                   cTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcac                         >  2:204333/1‑150 (MQ=255)
                                                                                                                   cTTTCGTCAGTAAGCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACTACTTGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcac                         <  2:277602/150‑1 (MQ=255)
                                                                                                                    tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcaca                        <  2:410286/150‑1 (MQ=255)
                                                                                                                    tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcaca                        >  2:1184057/1‑150 (MQ=255)
                                                                                                                               aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc             >  1:648228/1‑150 (MQ=255)
                                                                                                                               aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc             >  1:1531758/1‑150 (MQ=255)
                                                                                                                               aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc             >  1:1508214/1‑150 (MQ=255)
                                                                                                                                tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc                 <  1:437107/145‑1 (MQ=255)
                                                                                                                                tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc                 <  1:1550525/145‑1 (MQ=255)
                                                                                                                                tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc                 >  2:437107/1‑145 (MQ=255)
                                                                                                                                tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc                 >  2:1550525/1‑145 (MQ=255)
                                                                                                                                tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACAGGCGAAGTGCTGTGAATTTCAGCAACGCGTTAGCGCACACCAAACCAGcca            >  2:883309/1‑149 (MQ=255)
                                                                                                                                  caaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCtat          >  2:903381/6‑150 (MQ=255)
                                                                                                                                   gAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCtata         <  2:1267144/150‑1 (MQ=255)
                                                                                                                                      tttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAcc      <  1:1657690/150‑1 (MQ=255)
                                                                                                                                      attGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCTCACACCAAACCAGCCTATAAcc      >  1:1138507/2‑150 (MQ=255)
                                                                                                                                       ttGCCCGGCACGTAGCCGGGCAATTTTGCAATTACCTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGGGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCa     <  2:1079334/150‑1 (MQ=255)
                                                                                                                                       ttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCATCCAAAAACCa     >  2:813234/1‑150 (MQ=255)
                                                                                                                                        tGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAg    <  2:240952/150‑1 (MQ=255)
                                                                                                                                          cccGGCACGTAGCCTGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCa  <  1:1252827/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
ACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCA  >  NC_000913/1524315‑1524602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: