Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,171 Δ1 bp 6.0% coding (259/1035 nt) tqsA → pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,1710C. 6.0% 320.6 / 13.6 100coding (259/1035 nt)tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base C (42/52);  new base . (3/3);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

ATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGAC  >  NC_000913/1674024‑1674316
                                                                                                                                                   |                                                                                                                                                  
atactcGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTc                                                                                                                                                  <  1:239606/147‑1 (MQ=255)
  tctcGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCaa                                                                                                                                                <  1:512484/150‑1 (MQ=255)
   ctcGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAAc                                                                                                                                               <  1:1529920/150‑1 (MQ=255)
      gCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAg                                                                                                                                            >  2:365822/1‑150 (MQ=255)
      gCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAg                                                                                                                                            <  2:1304854/150‑1 (MQ=255)
        attattTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGtt                                                                                                                                          <  1:1633926/150‑1 (MQ=255)
         ttatttatTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTg                                                                                                                                         <  2:948686/150‑1 (MQ=255)
         ttatttatTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGATGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATTGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTg                                                                                                                                         <  2:198204/150‑1 (MQ=255)
          tatttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGa                                                                                                                                        <  1:1418519/150‑1 (MQ=255)
           atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAc                                                                                                                                      >  2:954978/1‑150 (MQ=255)
           atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAc                                                                                                                                      >  1:463947/1‑150 (MQ=255)
           atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAc                                                                                                                                      >  1:1701954/1‑150 (MQ=255)
              tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTTCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAcgcg                                                                                                                                   <  2:673033/150‑1 (MQ=255)
              tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGTCAACTCGTTGagcgcg                                                                                                                                   <  2:1672916/150‑6 (MQ=255)
              tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGTCAACTCGTTGagcgcg                                                                                                                                   <  1:57835/150‑6 (MQ=255)
               attGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGTTGCTATTAGCTTATCTGGGTTCCGCGTCAACTCGTTGAGCGCgg                                                                                                                                  <  1:47143/150‑1 (MQ=255)
                   cTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGt                                                                                                                              >  1:652882/1‑150 (MQ=255)
                    tGTTCTTCTTAACCCGCTGGGGCAACACCTGGTCCGCGGGCGTGTGCCGCGTGTACTGGCGGTGGCGATTTTGATGACCATCATCGTGAGGGCGATGGTGTTGCTATTAGCTGATCTGGGTTCCGCGCTCAACGCGTTGACGCGGACGtt                                                                                                                             <  2:835146/150‑1 (MQ=255)
                       tATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTAcc                                                                                                                          >  2:415533/1‑150 (MQ=255)
                        aTTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCg                                                                                                                         >  2:214301/1‑150 (MQ=255)
                          tCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACCGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCa                                                                                                                       >  1:1708207/1‑150 (MQ=255)
                            ttAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAat                                                                                                                     >  1:1365124/1‑150 (MQ=255)
                             tAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAata                                                                                                                    >  1:606574/1‑150 (MQ=255)
                             tAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAata                                                                                                                    >  2:1073495/1‑150 (MQ=255)
                              aaCCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAatat                                                                                                                   >  1:275881/1‑150 (MQ=255)
                                 ccGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGc                                                                                                                <  1:1691473/150‑1 (MQ=255)
                                    cTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAc                                                                                                             >  2:271602/1‑150 (MQ=255)
                                      ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCATTATCGCAActc                                                                                                           >  1:284411/1‑150 (MQ=255)
                                      ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAActc                                                                                                           >  2:683764/1‑150 (MQ=255)
                                      ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAActc                                                                                                           <  2:1465379/150‑1 (MQ=255)
                                         gCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGCCCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtat                                                                                                        >  2:1248036/1‑150 (MQ=255)
                                          cAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatt                                                                                                       >  2:746520/1‑150 (MQ=255)
                                           aaCCCATGGTCCGCTGGCGGGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGTGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta                                                                                                      <  2:240611/150‑1 (MQ=255)
                                           aaCACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGCCGTTAACGCAATATCGCAACTCtatta                                                                                                      >  2:931215/1‑150 (MQ=255)
                                           aaCACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta                                                                                                      <  2:1701954/150‑1 (MQ=255)
                                           aaCACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta                                                                                                      <  2:463947/150‑1 (MQ=255)
                                               caTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGATGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAc                                                                                                  <  2:1244940/150‑1 (MQ=255)
                                               aaTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAc                                                                                                  <  2:877883/149‑1 (MQ=255)
                                                aTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcg                                                                                                 <  1:1131677/150‑1 (MQ=255)
                                                aTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGATTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGAAGTTACCGCAATATCGCAACTAGATTATGAcg                                                                                                 >  2:210109/1‑150 (MQ=255)
                                                aTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGGCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcg                                                                                                 <  1:89855/150‑1 (MQ=255)
                                                          ggCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAg                                                                                       >  1:1424649/1‑150 (MQ=255)
                                                             gtgtGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAgcgc                                                                                    <  1:661894/150‑1 (MQ=255)
                                                             gtgtGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAgcgc                                                                                    <  2:1338883/150‑1 (MQ=255)
                                                              tgtgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCt                                                                                   <  1:124656/150‑1 (MQ=255)
                                                               gtgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCCTCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCCACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCtt                                                                                  <  1:543639/150‑1 (MQ=255)
                                                                tgCCGCGTGTACTGGCGGTGTCGATTTTGATGACGATCATCTTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCTCTCATCGAGTTGACGCGGACGTTACCGCAATATCGCTACTCTATTATGACGCCGCTGCAAGCGCTTg                                                                                 <  1:238532/150‑1 (MQ=255)
                                                                 gCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGAGATGCTGTTCCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTCACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAACCGCTTGa                                                                                >  2:1330389/1‑150 (MQ=255)
                                                                   cgcgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAAc                                                                              <  1:1731327/150‑1 (MQ=255)
                                                                   cgcgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAAc                                                                              >  1:223383/1‑150 (MQ=255)
                                                                   cgcgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCAATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCTAGCGCTTGAAc                                                                              <  1:271602/150‑1 (MQ=255)
                                                                     cgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGATCCg                                                                            >  2:1313645/1‑150 (MQ=255)
                                                                        gtACTGGCGGTGTCGATTTTGATGACCTTCATCGTGATGGCGATGGTGTTGATATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCTATATCGCAACTGTATTATGACGCCGCTGCAAGCGCTTGAACCgttg                                                                         <  1:554862/150‑1 (MQ=255)
                                                                        gtACTGGCGGTGGCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCgttg                                                                         <  2:908073/150‑1 (MQ=255)
                                                                           cTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGttgttg                                                                      >  1:1606397/1‑150 (MQ=255)
                                                                            tGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGc                                                                     <  1:404909/150‑1 (MQ=255)
                                                                            tGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGc                                                                     <  2:500740/150‑1 (MQ=255)
                                                                             ggCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCa                                                                    <  1:881921/150‑1 (MQ=255)
                                                                                   gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                              >  1:424355/1‑150 (MQ=255)
                                                                                   gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                              <  2:545753/150‑1 (MQ=255)
                                                                                   gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                              <  2:1608348/150‑1 (MQ=255)
                                                                                     cGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAAcg                                                                 >  1:62809/1‑145 (MQ=255)
                                                                                     cGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAAcg                                                                 <  2:62809/145‑1 (MQ=255)
                                                                                     cGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAg                                                            >  1:8508/1‑150 (MQ=255)
                                                                                        ttttGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGa                                                         >  1:249717/1‑150 (MQ=255)
                                                                                               gaCAATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGATACCGCAAGATCGCAACTCTTTTATGACGCCGCTGCAAGAGCGTGAAACGTTGGTGCAACGCGTAGGGATTGACGt                                                  >  2:131141/1‑150 (MQ=255)
                                                                                                 ccATCATCGTGATGGCGATGGTGTTGCTAATAGCTTATCTGGGTTCCGCGCTCAACGAGTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGtctc                                               <  1:129247/150‑1 (MQ=255)
                                                                                                      atcGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTAAAGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTAAGtt                                           >  1:238761/1‑150 (MQ=255)
                                                                                                        cGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGa                                         >  1:1004145/1‑150 (MQ=255)
                                                                                                         gTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATCTCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAc                                        <  1:1298239/150‑1 (MQ=255)
                                                                                                         gTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAc                                        <  2:665031/150‑1 (MQ=255)
                                                                                                         gTGAGGGCGATTGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGAGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAc                                        <  1:1159759/150‑1 (MQ=255)
                                                                                                          tGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAcc                                       <  2:1326501/150‑1 (MQ=255)
                                                                                                           gATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCa                                      >  2:448653/1‑150 (MQ=255)
                                                                                                            aTGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAg                                     <  1:954978/150‑1 (MQ=255)
                                                                                                            aTGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAg                                     <  2:275881/150‑1 (MQ=255)
                                                                                                             tGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGc                                    >  2:721359/1‑150 (MQ=255)
                                                                                                             tGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGc                                    >  1:1190514/1‑150 (MQ=255)
                                                                                                                  aTGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGgcg                               <  2:1287014/150‑1 (MQ=255)
                                                                                                                      tgtTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGAAGTGGGAGTTGACCAGCTGGCGCAtt                           >  1:954640/1‑150 (MQ=255)
                                                                                                                          gCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTatat                       >  2:1314707/1‑150 (MQ=255)
                                                                                                                           cTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATAtt                      <  2:987718/150‑1 (MQ=255)
                                                                                                                           cTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATAtt                      <  2:1265170/150‑1 (MQ=255)
                                                                                                                           cTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCGTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATAtt                      <  2:1711201/150‑1 (MQ=255)
                                                                                                                            tATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGCCGTCTCAGTTGACCAGCTGGCGCAGTATATTg                     >  2:125908/1‑150 (MQ=255)
                                                                                                                             aTTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCCACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGa                    >  2:1608446/1‑150 (MQ=255)
                                                                                                                               tAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATAACGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATc                  <  2:826480/150‑1 (MQ=255)
                                                                                                                                aGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                 <  1:948358/150‑1 (MQ=255)
                                                                                                                                aGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                 <  2:1250391/150‑1 (MQ=255)
                                                                                                                                aGCTTATCTGGGATCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                 <  2:312376/150‑1 (MQ=255)
                                                                                                                                 gCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCg                <  2:50269/150‑1 (MQ=255)
                                                                                                                                       gtaacgtCCGCGTCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAAcgcg          >  2:1140636/7‑150 (MQ=255)
                                                                                                                                          ggTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGGAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggcg       >  1:1068914/1‑150 (MQ=255)
                                                                                                                                          ggTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggcg       <  1:365822/150‑1 (MQ=255)
                                                                                                                                           gTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCGAACGCGGCGAt     >  1:1662725/1‑150 (MQ=255)
                                                                                                                                           gTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCGCAGTTGACCAGCTGGCGCATTATATTGATCGAACGCGGCGAt     >  1:1669170/1‑150 (MQ=255)
                                                                                                                                           cgtCCGCGTCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGa      >  2:1692312/3‑150 (MQ=255)
                                                                                                                                           cgtCCGCGTCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGa      >  1:513672/3‑150 (MQ=255)
                                                                                                                                              ccGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGa   >  2:1253677/1‑150 (MQ=255)
                                                                                                                                               cgcgCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGGTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGAc  <  2:284411/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGAC  >  NC_000913/1674024‑1674316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: