Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,121,583 Δ1 bp 7.7% intergenic (‑29/+26) wcaJ ← / ← cpsG colanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,121,5830G. 7.7% 256.4 / 18.6 91intergenic (‑29/+26)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (38/45);  new base . (3/4);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

CGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAG  >  NC_000913/2121434‑2121732
                                                                                                                                                     |                                                                                                                                                      
cGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCg                                                                                                                                                        >  2:97207/1‑150 (MQ=255)
 gCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCTTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGc                                                                                                                                                       >  1:893394/1‑150 (MQ=255)
 gCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGc                                                                                                                                                       >  2:488895/1‑150 (MQ=255)
     aCTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTg                                                                                                                                                   <  2:385001/150‑1 (MQ=255)
      cTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgg                                                                                                                                                 <  2:717384/150‑1 (MQ=255)
      cTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgg                                                                                                                                                 <  1:1602808/150‑1 (MQ=255)
       ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTACTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                                >  1:1033723/1‑150 (MQ=255)
         cGCAAACCAGCCATTGTGCGGCAAACCTCATGCGGATATCTGAAACGCCTCGCACCCTAGAGATTAACGAAGCATTTGTTTTCGCACGCTCGCGCTTTTTAACATTTGTCATCGTTGTTCCTGTTATTAGCCCCTAACCCGCAGTgggct                                                                                                                                              <  2:1149711/150‑3 (MQ=255)
         cGCAAACCAGCCATAGTCCGGCAAACATGATGGGGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTATTATTAGCCCCTTACCCGCAGTGGGGt                                                                                                                                              <  2:710605/150‑1 (MQ=255)
          gCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTa                                                                                                                                             <  2:146231/150‑1 (MQ=255)
             aaCCAGCCATAGTCCGGCCAACATGATGGAGATATCTGAAAAGCGTTGCACCATAGAGATTAACGTTGCATTGGTTTTCGCTCGCTCGCGCGTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAgg                                                                                                                                          <  2:317608/150‑1 (MQ=255)
             aaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAgg                                                                                                                                          >  2:1616343/1‑150 (MQ=255)
               ccAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAgggg                                                                                                                                        <  1:1179301/150‑2 (MQ=255)
                 aGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAg                                                                                                                                      >  2:222934/1‑150 (MQ=255)
                 aGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAg                                                                                                                                      >  2:112607/1‑150 (MQ=255)
                   ccATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGAt                                                                                                                                    <  1:768181/150‑1 (MQ=255)
                    cATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATc                                                                                                                                   >  2:1653805/1‑150 (MQ=255)
                    cATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggggctaat                                                                                                                                   <  1:381989/150‑7 (MQ=255)
                     aTAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATcc                                                                                                                                  <  2:1096408/150‑1 (MQ=255)
                     aTAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATcc                                                                                                                                  <  1:487729/150‑1 (MQ=255)
                        gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggggc                                                                                                                                       <  2:1687432/142‑3 (MQ=255)
                        gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggggc                                                                                                                                       >  1:1687432/1‑140 (MQ=255)
                         tCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTGTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACa                                                                                                                              <  1:1106852/150‑1 (MQ=255)
                           cGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGACCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACAtt                                                                                                                            >  1:1530825/1‑150 (MQ=255)
                             gCAAACATGAGGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTGTTCGCGCGCTCGCGCTTTGTAAGATTTGTCATCGTTGTTCCTGTTCTTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTAc                                                                                                                          <  1:95766/150‑1 (MQ=255)
                              cAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGCAGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCTCAGTGGGGTAAGGGAAGATCCGACATTACt                                                                                                                         <  1:641560/150‑1 (MQ=255)
                                   atgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTc                                                                                                                    <  2:814980/150‑1 (MQ=255)
                                     gatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAg                                                                                                                  <  1:1660055/150‑1 (MQ=255)
                                     gatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAg                                                                                                                  >  1:1473438/1‑150 (MQ=255)
                                                cTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagca                                                                                                       >  2:251007/1‑150 (MQ=255)
                                                 tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCTCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                      <  1:1318970/150‑1 (MQ=255)
                                                 tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                      >  2:1140506/1‑150 (MQ=255)
                                                 tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                      <  1:1239393/150‑1 (MQ=255)
                                                   aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCagag                                                                                                    <  1:376463/150‑1 (MQ=255)
                                                   aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATACGACATTACTCGTTCAGCCACGTCAGCagag                                                                                                    >  1:856025/1‑150 (MQ=255)
                                                    aaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgt                                                                                                   <  1:416272/150‑1 (MQ=255)
                                                    aaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTAGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgt                                                                                                   >  1:292416/1‑150 (MQ=255)
                                                        cGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgc                                                                                               >  2:964514/1‑150 (MQ=255)
                                                              aCCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                         <  1:512260/150‑1 (MQ=255)
                                                                 aTAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTTGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCAGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGttcgcgttcgcgat                                                                                      >  1:875293/1‑148 (MQ=255)
                                                                 aTAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTACTGTTATTAGCACCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCt                                                                                      >  2:562836/1‑150 (MQ=255)
                                                                    gagaTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTcc                                                                                   <  2:1232772/150‑1 (MQ=255)
                                                                    gagaTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTcc                                                                                   <  2:1406080/150‑1 (MQ=255)
                                                                     agaTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCa                                                                                  >  1:395586/1‑150 (MQ=255)
                                                                      gaTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCAt                                                                                 >  1:1190179/1‑150 (MQ=255)
                                                                       aTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATc                                                                                <  1:1596312/150‑1 (MQ=255)
                                                                         tAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAg                                                                              <  2:1285243/150‑1 (MQ=255)
                                                                                gaaTTGGTATTCGCTCGCTCGCGATTTTTTAGATTTGTCATCGTTGTTCCTGTAATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGAcaca                                                                       <  2:319965/148‑1 (MQ=255)
                                                                                 cATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACAt                                                                      >  1:177930/1‑150 (MQ=255)
                                                                                 cATTGGTTTTCGCTCGATCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACAt                                                                      >  1:71196/1‑150 (MQ=255)
                                                                                  aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCTGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATc                                                                     <  1:1422097/150‑1 (MQ=255)
                                                                                  aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATc                                                                     >  1:923175/1‑150 (MQ=255)
                                                                                       ttttCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgc                                                                <  2:1260226/150‑1 (MQ=255)
                                                                                          tcgctcgctcgcGCTTTTTTATATTTGTCTTCGTTGTTCCTGTTATAAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgcgcg                                                             <  1:323612/150‑1 (MQ=255)
                                                                                          tcgctcgctcgcGCTGTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgcgcg                                                             <  1:279905/150‑1 (MQ=255)
                                                                                            gctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                           >  2:1441541/1‑150 (MQ=255)
                                                                                            gctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                           >  2:1379289/1‑150 (MQ=255)
                                                                                             ctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAtt                                                          >  2:1233406/1‑150 (MQ=255)
                                                                                                gctggcGGTTTTTTAGATTTGTCATCGTTGTTCCTGTTTTTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCca                                                       <  2:1011659/150‑1 (MQ=255)
                                                                                                gctcgcGCTTTTTTAGATTTGTCATCGTGGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCca                                                       <  2:1066571/150‑1 (MQ=255)
                                                                                                 cgcgcgcgTTTTTAGATTTGTCATCGTTGTTCCTGTTCTTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCcac                                                      <  1:341678/148‑1 (MQ=255)
                                                                                                     cgcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTc                                                  >  1:1356559/1‑150 (MQ=255)
                                                                                                     cgcTTTTTTAGATTTGTCATCGGTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTCCTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTc                                                  <  2:866866/150‑1 (MQ=255)
                                                                                                     cgcTGTTTTAGATTTGTCATCGTTGTTCCTGTTATAAGCCCCTTCCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCCACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTc                                                  <  2:872449/150‑1 (MQ=255)
                                                                                                       cTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                                >  2:662143/1‑150 (MQ=255)
                                                                                                       cTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                                >  1:940495/1‑150 (MQ=255)
                                                                                                       cTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                                >  2:1526850/1‑150 (MQ=255)
                                                                                                        ttttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAgg                                               >  2:118277/1‑150 (MQ=255)
                                                                                                        ttttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAgg                                               <  1:488895/150‑1 (MQ=255)
                                                                                                         tttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGTTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGgc                                              <  2:1033723/150‑1 (MQ=255)
                                                                                                                      cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGTTCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                                 <  1:1397621/150‑1 (MQ=255)
                                                                                                                             gttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTg                          <  1:964514/150‑1 (MQ=255)
                                                                                                                              ttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCATATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTgg                         <  2:1116701/150‑1 (MQ=255)
                                                                                                                              ttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTgg                         <  1:1566691/150‑1 (MQ=255)
                                                                                                                                ccTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg                       <  1:598925/150‑1 (MQ=255)
                                                                                                                                 cTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                      <  2:1410439/150‑1 (MQ=255)
                                                                                                                                 cTGGTAATACCCCCTTACCCGCAGGGGGGTAAGGGCAGATCCGACAATAATCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGGTTCCATCAGCGGCACAACACCGCGCGAATCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                      <  1:1639591/150‑1 (MQ=255)
                                                                                                                                   gTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTg                    <  2:177930/150‑1 (MQ=255)
                                                                                                                                         aGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGTGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg              >  2:1170925/1‑150 (MQ=255)
                                                                                                                                         aGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg              <  1:112607/150‑1 (MQ=255)
                                                                                                                                          gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt             >  2:1019189/1‑150 (MQ=255)
                                                                                                                                          gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt             >  1:902500/1‑150 (MQ=255)
                                                                                                                                          gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt             <  1:110825/150‑1 (MQ=255)
                                                                                                                                           tcccTTACCCCACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt            >  1:1150099/2‑150 (MQ=255)
                                                                                                                                           ccccTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt            >  2:1481248/1‑150 (MQ=255)
                                                                                                                                            cccTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTa           >  1:1530475/1‑150 (MQ=255)
                                                                                                                                            cccTTACCCCACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTa           >  1:341449/1‑150 (MQ=255)
                                                                                                                                                tACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgc       >  1:31494/1‑150 (MQ=255)
                                                                                                                                                 aCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCCCCACCGGTTCGGTATTGGAGGTGCGCAGGTTACAgcg      >  1:159104/1‑150 (MQ=255)
                                                                                                                                                  cccGCAGTGAGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc     >  2:1666494/1‑150 (MQ=255)
                                                                                                                                                   ccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGcc    <  2:1530825/150‑1 (MQ=255)
                                                                                                                                                   ccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGcc    >  2:943643/1‑150 (MQ=255)
                                                                                                                                                     gCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAg  <  1:1616343/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAG  >  NC_000913/2121434‑2121732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: