Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,224:1 +A 5.8% intergenic (+32/‑211) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2241.A 5.8% 612.7 / 29.2 156intergenic (+32/‑211)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base . (74/73);  new base A (5/4);  total (79/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

Only 100 of 159 total aligned reads displayed.

GAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCA  >  NC_000913/3888076‑3888373
                                                                                                                                                     |                                                                                                                                                     
gAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGa                                                                                                                                                      >  1:102251/1‑150 (MQ=255)
   ctgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                                                                   >  1:1483645/1‑150 (MQ=255)
    tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTACAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaatgcg                                                                                                                                                 <  2:1289962/150‑6 (MQ=255)
      ctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCAGCATTCTGACTGTCAGATGCGgc                                                                                                                                                >  2:283469/1‑150 (MQ=255)
      cgggCGGAAGAGTTGCGTCTGGCACAGCAGCACGTACGCGAAATCACCGGGGAAGTGACTTCAGCCGACCTGCTGGGGCGGAGGTTGTCCAGCTTCTGTATTGGAAAGTTACCGCGCTTTCGAAGCCGCATTCTGACGGTCAGATGCGgc                                                                                                                                                >  2:283466/3‑150 (MQ=255)
       gggCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                                                                               <  1:1631598/149‑1 (MQ=255)
         gCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTCTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttc                                                                                                                                             <  1:476080/150‑1 (MQ=255)
          cGGAATCGTTGCGTCTGGCACAGCAGAACTTACTCTAAATCACAGTGGAATTAACTTCAGACGACCTGCTGGTGCTGATTTTCTCCATCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgcttcg                                                                                                                                            <  2:1146735/150‑1 (MQ=255)
          cGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAACTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                                                                            >  1:1546402/1‑150 (MQ=255)
          cGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                                                                            <  2:1042325/150‑1 (MQ=255)
          cGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgcttcg                                                                                                                                            <  2:1401820/150‑1 (MQ=255)
            gAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgct                                                                                                                                          <  1:1566858/150‑1 (MQ=255)
             aaGAGTTGCGTCTGGCACAGCACAACTTAAGCGAAATCACCGGGTAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                                                                                         <  1:316702/150‑1 (MQ=255)
                 gTTGCGTCTGGCACAGCTGAACTTAAGCGTTTTCACGGGGGAATTTACTTCAGACGACCTTCTGGGGCGGATTTTCTCGAGCTTCTGTTTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                                                                     <  2:118250/150‑1 (MQ=255)
                       tCTGGCACAGCATAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGTATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                                                                               <  2:1433043/150‑1 (MQ=255)
                          ggCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACt                                                                                                                            >  2:782264/1‑150 (MQ=255)
                           gCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTc                                                                                                                           >  1:742546/1‑150 (MQ=255)
                               agcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                                                                       >  2:722298/1‑150 (MQ=255)
                               agcagAACTTAAGCGAAATCACCGGGGAATTTAATTCAGACTACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGAGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                                                                       <  2:252388/150‑1 (MQ=255)
                                gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGtt                                                                                                                      >  2:1366062/1‑150 (MQ=255)
                                gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGtt                                                                                                                      >  1:619266/1‑150 (MQ=255)
                                      cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                                                                                >  2:964427/1‑150 (MQ=255)
                                      cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTAATTCCt                                                                                                                >  1:1708761/1‑150 (MQ=255)
                                          aGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                                                                            >  1:161236/1‑150 (MQ=255)
                                           gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                                                                           <  2:1483645/150‑1 (MQ=255)
                                            cGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAccc                                                                                                          >  1:432300/1‑150 (MQ=255)
                                              aaaTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtt                                                                                                        >  1:1353127/1‑150 (MQ=255)
                                                 tCACCGGGGAATTTACTTCAGCCGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttt                                                                                                     <  2:1725064/150‑1 (MQ=255)
                                                     cGGGGAATTTACTTCAGACGACCTGCTGGGGCTGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                                                                                 >  2:1379425/1‑150 (MQ=255)
                                                       gggTATTTAATTCAGACGACCTGGTGGGGCTGATTTTCTCCAGCTTCTGTCTTGGTAAGTAAACGCGATTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACACTTTTTTTAAAc                                                                                               <  1:850523/150‑1 (MQ=255)
                                                          aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACAtt                                                                                            >  2:731580/1‑150 (MQ=255)
                                                              tACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                                                                        <  1:1149172/150‑1 (MQ=255)
                                                                cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCCACCCTTTTTTTAAACATTAAAAtt                                                                                      >  2:259825/1‑150 (MQ=255)
                                                                  tCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCt                                                                                    >  1:1341528/1‑150 (MQ=255)
                                                                   cAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt                                                                                   >  2:1204921/1‑150 (MQ=255)
                                                                    aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCTCATTCTGACTGTCAGATGCTTCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                                                                                  <  1:722298/150‑1 (MQ=255)
                                                                          ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                                                                            >  2:1681558/1‑150 (MQ=255)
                                                                          ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                                                                            >  1:1261248/1‑150 (MQ=255)
                                                                          ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                                                                            >  1:1426351/1‑150 (MQ=255)
                                                                           ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAGCCGCGCTTACGAAGCCGCATTCTGAGGGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCCACCCTTTTTTTAAACATTAAAATTCTTACGTAAtt                                                                           >  1:1187768/1‑150 (MQ=255)
                                                                           ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAtt                                                                           >  2:1704690/1‑150 (MQ=255)
                                                                                ggggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATaa                                                                      >  2:820457/1‑150 (MQ=255)
                                                                                 gggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCCCTCCTGTTTTTCCTCAACCCTTTTTTTTAACATTAAAATTCTTACGTAATTTATAAt                                                                     >  2:928433/1‑150 (MQ=255)
                                                                                  ggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCt                                                                   >  2:418702/1‑150 (MQ=255)
                                                                                    cGGATTTTCTCCAGCTTCTTTATTTTTAAGTAAACTATCTTACGAAGCCTCATTCTTACTGTCATATTATGATTAGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCtt                                                                  <  1:967784/150‑1 (MQ=255)
                                                                                    cGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCtt                                                                  <  2:877455/150‑1 (MQ=255)
                                                                                     ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCttt                                                                 <  2:1344275/150‑1 (MQ=255)
                                                                                      taTTTTCTCCAGCTTCTGTATTGGTATGAAACCGCGCTTACGAAGCGGCATTCTGAATGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa                                                                <  1:1102340/149‑1 (MQ=255)
                                                                                      taTTTTCTCCAGCTTCTGTATTGGTAAGTAACCTCTCTTACGAATCCGCATTCTGACTGTCAGATGCGGATTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa                                                                <  2:77223/149‑1 (MQ=255)
                                                                                      gATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa                                                                >  1:429130/1‑150 (MQ=255)
                                                                                      gATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa                                                                <  2:1594163/150‑1 (MQ=255)
                                                                                       aTTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa                                                               <  1:782264/150‑1 (MQ=255)
                                                                                       aTTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa                                                               <  2:1130840/150‑1 (MQ=255)
                                                                                       aTTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa                                                               >  1:486561/1‑150 (MQ=255)
                                                                                            ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAATGTCTTACGTAATTTATAATCTTTaataaaa                                                          >  2:894060/1‑150 (MQ=255)
                                                                                             tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTATAATTCTTACGTAATTTATAATCTTTAAAAAAAg                                                         >  1:821296/1‑150 (MQ=255)
                                                                                             tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTAGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg                                                         <  1:892472/150‑1 (MQ=255)
                                                                                              ccAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCTCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAAAATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc                                                        >  2:484188/1‑150 (MQ=255)
                                                                                                  cTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAttt                                                    >  1:394175/1‑150 (MQ=255)
                                                                                                  cTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACACTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAttt                                                    <  1:267188/150‑1 (MQ=255)
                                                                                                    tCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTaa                                                  >  1:274749/1‑150 (MQ=255)
                                                                                                         tttGGTTAGTAACCGAGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTg                                             <  1:1062065/149‑1 (MQ=255)
                                                                                                         aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCATCAACCCTTTTTTTAAAAATTAAAATTCTTCCGTATTTTATAATCTTTAAAAAAAGCATTTAATATTg                                             >  2:1321838/1‑150 (MQ=255)
                                                                                                          ttGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTga                                            >  2:1681927/1‑149 (MQ=255)
                                                                                                          ttGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGc                                            <  2:1549186/150‑1 (MQ=255)
                                                                                                            ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCTGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTAATAATCTTTAAAAAAAGCATTTAATATTGCTc                                          <  1:227319/150‑1 (MQ=255)
                                                                                                            ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATACTTACGTAATTTATAATCTTTAAAAAAAGCAATTAATATTGCta                                          >  1:173534/1‑149 (MQ=255)
                                                                                                              taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTccc                                        >  1:827960/1‑150 (MQ=255)
                                                                                                               aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTcccc                                       >  1:1392299/1‑150 (MQ=255)
                                                                                                               aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTcccc                                       >  1:866560/1‑150 (MQ=255)
                                                                                                               aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGATcccc                                       >  1:938736/1‑150 (MQ=255)
                                                                                                                 gtaaCCGCGCTTACGAAGCCGCATTCTGTCTGTCAGATGCGGCTTCGCTTCATTGTTAGCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGa                                     <  2:221602/150‑1 (MQ=255)
                                                                                                                   aaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc                                   >  1:691824/1‑150 (MQ=255)
                                                                                                                      cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGAt                                <  2:1375444/150‑1 (MQ=255)
                                                                                                                          cTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATtgtg                            <  1:1727718/150‑1 (MQ=255)
                                                                                                                            tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgat                          >  1:129433/1‑150 (MQ=255)
                                                                                                                             gcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCATCCATTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgatt                         >  1:1169043/2‑150 (MQ=255)
                                                                                                                             aCGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgatt                         <  2:541899/150‑1 (MQ=255)
                                                                                                                             aCGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgatt                         <  2:692486/150‑1 (MQ=255)
                                                                                                                              cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc                        <  1:1704690/150‑1 (MQ=255)
                                                                                                                              cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc                        <  2:742546/150‑1 (MQ=255)
                                                                                                                              cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc                        <  2:429130/150‑1 (MQ=255)
                                                                                                                              cGAAGCCGCATTCTGACCGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc                        <  2:171148/150‑1 (MQ=255)
                                                                                                                              cGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgtttc                        >  2:883835/1‑150 (MQ=255)
                                                                                                                              cGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc                        >  1:492073/1‑150 (MQ=255)
                                                                                                                                 aGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcgat                     <  2:93553/150‑1 (MQ=255)
                                                                                                                                  gCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcgatt                    >  2:1467421/1‑150 (MQ=255)
                                                                                                                                   ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATAGTTACCACTCGTGGTATTCCTCAACCCTTTTTTTAAACAATAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTAAATATTGCTCCCCGAACGATTGTgattcgattc                   >  1:290681/1‑150 (MQ=255)
                                                                                                                                     gCATTCTGACTGTCATATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTcac                 <  2:1286080/150‑1 (MQ=255)
                                                                                                                                      cATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTAAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTcaca                >  2:1630648/1‑150 (MQ=255)
                                                                                                                                       aTTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTACTCAACCCTTTTTTTAATCATTAATATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACAt               <  2:846417/150‑1 (MQ=255)
                                                                                                                                         tCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACAttt             <  2:124100/150‑1 (MQ=255)
                                                                                                                                          cTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTaa           <  1:418702/150‑1 (MQ=255)
                                                                                                                                           tGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTaa           >  1:992513/1‑150 (MQ=255)
                                                                                                                                            gACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTaaa          >  1:440238/1‑150 (MQ=255)
                                                                                                                                             agtGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAAc         <  2:102251/148‑1 (MQ=255)
                                                                                                                                                gTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAAt      <  1:1005220/150‑1 (MQ=255)
                                                                                                                                                 tCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAAtt     >  2:685564/1‑150 (MQ=255)
                                                                                                                                                  cAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAAttt    <  2:619266/150‑1 (MQ=255)
                                                                                                                                                    gATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCa  >  1:15826/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCA  >  NC_000913/3888076‑3888373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: