Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,970,817:1 +G 5.5% coding (685/1131 nt) wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,8171.G 5.5% 454.2 / 19.3 110coding (685/1131 nt)wecBUDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (57/47);  new base G (3/3);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

Only 100 of 113 total aligned reads displayed.

TGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTG  >  NC_000913/3970671‑3970965
                                                                                                                                                   |                                                                                                                                                    
tGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGCCCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGCAATCTGCCCCGCGCTgg                                                                                                                                                   >  1:168068/1‑150 (MQ=255)
tGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTgg                                                                                                                                                   <  1:1215308/150‑1 (MQ=255)
    tGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGCCCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGa                                                                                                                                               >  1:497581/1‑150 (MQ=255)
      aCCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCAGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACa                                                                                                                                             <  2:907719/150‑1 (MQ=255)
       ccAGGTGATGAGCAGCGACAAGCTGGGTACAGAACTGGCGGCAAATTACCCGTTTATCTACGCCGATAAAAAGATGATTCTGGTGCCCGGTCACAGGATTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACAt                                                                                                                                            >  2:69772/1‑150 (MQ=255)
        cAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCAGAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAGTCTGCCAGCGCGTGGCAGatttc                                                                                                                                           <  2:760732/150‑5 (MQ=255)
        cAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATc                                                                                                                                           >  1:1429715/1‑150 (MQ=255)
        cAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGGTGCGGGCGTGGCTTTGAAGAAATCTGCCAGCGCGTGGCAGatttc                                                                                                                                           <  1:15815/150‑5 (MQ=255)
           gTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGCTAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAGTCTGCCAGCGCGTGGCAGatttcttc                                                                                                                                        <  1:927505/150‑8 (MQ=255)
           gTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGcc                                                                                                                                        <  2:1299102/150‑1 (MQ=255)
           gTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGcc                                                                                                                                        <  1:1286032/150‑1 (MQ=255)
               tgaGCAGCGACAAGCTGCGTTCAGAACTGGCGGCTAATTACCCGTTTATCGACCCCGATATAAAGATGATTCTGGTGTCCGGTCACAGGGGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGTCATCGccacca                                                                                                                                    >  1:942615/1‑150 (MQ=255)
               tgaGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGCCCACGATAAAACGATGATTCTGGTGACCGGTCACAGGCGTGCGAGTTTCGGTCGTGGGTTTGAAGAAATCTGCCACGCGCTGTCAGACCTCGcccccc                                                                                                                                    >  1:859321/1‑149 (MQ=255)
                gaGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCcaccac                                                                                                                                   <  1:1032377/150‑1 (MQ=255)
                  gcagcGACAAGCTGCGTTCAGAACTGGCGGCAAATTTCCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGATAGTTTGGGTCGTGGCTTTGAAGAAATCTGCCACGCGCGGGCTGACATCGCcaccactc                                                                                                                                 <  2:403435/150‑3 (MQ=255)
                     gcGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCCCCACGCAcc                                                                                                                              >  2:938104/1‑150 (MQ=255)
                     gcGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCAcc                                                                                                                              >  2:39345/1‑150 (MQ=255)
                       gACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAg                                                                                                                            >  1:1164481/1‑150 (MQ=255)
                        aCAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAgg                                                                                                                           <  1:407093/150‑1 (MQ=255)
                          aaGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGAc                                                                                                                         <  2:1375886/150‑1 (MQ=255)
                              tGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATcc                                                                                                                     >  1:1035713/1‑150 (MQ=255)
                                 gTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATCATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGa                                                                                                                  >  2:183099/1‑150 (MQ=255)
                                       aaCTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCt                                                                                                            <  1:675683/150‑1 (MQ=255)
                                        aCTGGCGGCAACTTCCCCGTTTATCGACCCCGATAAAAAGATGATGCTGGTGACCGGTCACAGGCGGGAGAGAGTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCGGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTa                                                                                                           <  1:1076899/150‑1 (MQ=255)
                                         cTGGCGGCAAATTACCCGTTTATCGACCCCGCTAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTAt                                                                                                          <  1:662115/150‑1 (MQ=255)
                                           ggcggcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATcc                                                                                                        >  1:453357/1‑150 (MQ=255)
                                             cggcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCgg                                                                                                      <  1:1679766/150‑1 (MQ=255)
                                              ggcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGt                                                                                                     >  2:620721/1‑150 (MQ=255)
                                              ggcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGt                                                                                                     >  1:117360/1‑150 (MQ=255)
                                               gcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTg                                                                                                    <  1:741462/150‑1 (MQ=255)
                                                cAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGc                                                                                                   <  2:1010677/150‑1 (MQ=255)
                                                cAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGc                                                                                                   >  1:534729/1‑150 (MQ=255)
                                                 aaaTTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCa                                                                                                  >  2:1264261/1‑150 (MQ=255)
                                                 aaaTTACACGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACAACCAGATTGTCTATACGGTGCa                                                                                                  >  2:110223/1‑150 (MQ=255)
                                                  aaTTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCAt                                                                                                 <  2:1038512/150‑1 (MQ=255)
                                                   aTTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCAtc                                                                                                <  1:1245640/150‑1 (MQ=255)
                                                    ttACCCGTTTATCGACCCCGCTAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGGTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCAtct                                                                                               <  2:349774/150‑1 (MQ=255)
                                                    ttACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCAtct                                                                                               <  1:1086989/150‑1 (MQ=255)
                                                       cccGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCaa                                                                                            >  1:172765/1‑150 (MQ=255)
                                                       cccGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCaa                                                                                            >  2:781925/1‑150 (MQ=255)
                                                         cGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAAcc                                                                                          >  2:621146/1‑150 (MQ=255)
                                                               tCGCCCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGAGAGACTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCCCCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACg                                                                                    >  2:341804/1‑150 (MQ=255)
                                                                cGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGt                                                                                   <  2:1268323/150‑1 (MQ=255)
                                                                 gACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTc                                                                                  >  1:730023/1‑150 (MQ=255)
                                                                 gACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTc                                                                                  >  1:723509/1‑150 (MQ=255)
                                                                   ccccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCag                                                                                >  1:576061/1‑150 (MQ=255)
                                                                    cccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCaga                                                                               <  1:1266797/150‑1 (MQ=255)
                                                                    cccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCaga                                                                               >  1:494531/1‑150 (MQ=255)
                                                                     ccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCagag                                                                              <  1:557665/150‑1 (MQ=255)
                                                                           aaaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCgg                                                                        <  2:1385277/150‑1 (MQ=255)
                                                                            aaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                       >  2:1504771/1‑150 (MQ=255)
                                                                            aaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                       >  2:1209858/1‑150 (MQ=255)
                                                                              aGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCa                                                                     >  1:650102/1‑150 (MQ=255)
                                                                              aGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTGTCGGTCGTGGCTTTGAAGAAATCTGCCCCGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGGCTATCCGGTGCATCTCAACCCGACGGTCACAGCACCGGTCa                                                                     >  2:323047/1‑150 (MQ=255)
                                                                                 tgatTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                                  <  2:698147/150‑1 (MQ=255)
                                                                                   atTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACAGCGCCACCACGCACCAGGCCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCCATCGc                                                                >  2:250055/1‑150 (MQ=255)
                                                                                       tGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCTGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTc                                                            <  2:1213935/150‑1 (MQ=255)
                                                                                       tGGGGACCGGGCACAGGCGGGAGAGTTTCGGGCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCAGCAGGACATCCAGATTGTCTATCCGGTGCTTCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTc                                                            <  2:1026868/150‑1 (MQ=255)
                                                                                          tGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTgg                                                         >  1:1151192/1‑150 (MQ=255)
                                                                                           gACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTggg                                                        <  1:818684/150‑1 (MQ=255)
                                                                                               ggTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCAt                                                    >  2:1696828/1‑150 (MQ=255)
                                                                                                    caGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATACGGTGCATCTCAACCCGAACGTCAGAGAACCGGGCAATCGCATTCTGGGGCATGTGaa                                               >  2:1604268/1‑150 (MQ=255)
                                                                                                      ggCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaaa                                             >  1:1653896/1‑150 (MQ=255)
                                                                                                        cGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaa                                              >  2:624144/1‑147 (MQ=255)
                                                                                                        cGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaa                                              <  1:624144/147‑1 (MQ=255)
                                                                                                        cGTGAGAGTTGCGGGCGCGGCTTTGAAGAAATCTGCCACGCGCGGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGGCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAAt                                           <  2:761714/150‑1 (MQ=255)
                                                                                                         gTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCACCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATg                                          >  2:1627546/1‑150 (MQ=255)
                                                                                                         gTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGACACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAGCCCGAACGTCAGAGAACCGGTGATTAGCATTCTGGGGCATGTGaaccatt                                          >  1:89545/1‑145 (MQ=255)
                                                                                                            agagTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATACGGTGCATCTCAACCCGAACGTCAGAGGACCGGTCAATCGCATTCTGGGGCATGTTAAAAATGTCa                                       >  2:198863/1‑150 (MQ=255)
                                                                                                              agTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAtt                                     >  1:744839/1‑150 (MQ=255)
                                                                                                               gTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTc                                    <  2:160366/150‑1 (MQ=255)
                                                                                                                     gTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGCCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcg                              <  1:1432495/150‑1 (MQ=255)
                                                                                                                     gTCGTGGCTTAGAAGAAATCTGCCACGCGCTGGCAGACATGGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGATCGTCAGAGAACCGGTCAAACGCATTCTGTGGCATGTGAAAAATGTCATTCTgatcg                              <  1:826573/150‑1 (MQ=255)
                                                                                                                        gTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcgatc                           >  1:1322423/1‑150 (MQ=255)
                                                                                                                         ttgatttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATAGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATcc                          <  2:276789/146‑1 (MQ=255)
                                                                                                                         tGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATcc                          <  1:1689919/150‑1 (MQ=255)
                                                                                                                          ggCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATccc                         >  1:1034/1‑150 (MQ=255)
                                                                                                                          ggCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATccc                         >  1:486545/1‑150 (MQ=255)
                                                                                                                            cTTTGACGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGCCTAACCGCTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCa                       <  2:1429715/150‑1 (MQ=255)
                                                                                                                             tatGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCGAg                      >  1:31143/3‑150 (MQ=255)
                                                                                                                              ttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGACCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                     >  1:1232744/1‑150 (MQ=255)
                                                                                                                              ttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                     <  1:1504771/150‑1 (MQ=255)
                                                                                                                              ttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                     >  1:916184/1‑150 (MQ=255)
                                                                                                                              ttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                     >  1:798785/1‑150 (MQ=255)
                                                                                                                               tGAAGAAATCTGCCCCGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCCACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAACTGTCATTCTGATCGATCCCCAGGa                    >  1:1733313/1‑150 (MQ=255)
                                                                                                                                ggcgatgtCTGCCAGCGCGTGGCAGACATCGCCACCACGCACCAGGACATCGAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAg                   >  1:342104/8‑150 (MQ=255)
                                                                                                                                gaagaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGTCATCCAGATTGTCTATCCGGTGCATCTCACCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAATAATGTCATTCTGCTCGATCCCCAGGAg                   >  2:52904/1‑150 (MQ=255)
                                                                                                                                gaagaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAg                   >  2:669395/1‑150 (MQ=255)
                                                                                                                                 aagaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCACCAGGAGt                  >  2:1160047/1‑150 (MQ=255)
                                                                                                                                   taaaTCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGTCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAt                <  2:856416/149‑1 (MQ=255)
                                                                                                                                   gaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAt                <  1:938104/150‑1 (MQ=255)
                                                                                                                                    atgtCTGCCAGCGCGTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAtt               >  1:1221320/4‑150 (MQ=255)
                                                                                                                                    aaaTCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAtt               >  2:412253/1‑150 (MQ=255)
                                                                                                                                        cTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTAcc           <  1:239151/150‑1 (MQ=255)
                                                                                                                                          gCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGTTTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGt         <  2:660446/150‑1 (MQ=255)
                                                                                                                                          gCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCATTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGt         >  1:1563555/1‑150 (MQ=255)
                                                                                                                                              cgcgCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGt     <  1:183099/150‑1 (MQ=255)
                                                                                                                                               gcgcTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGACCGTCAGAGCACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTc    >  1:1199075/1‑150 (MQ=255)
                                                                                                                                               gcgcTGGCAGACATCGACACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATACTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTc    <  2:908764/150‑1 (MQ=255)
                                                                                                                                                 gcTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAATCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTg  <  2:117360/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTG  >  NC_000913/3970671‑3970965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: