Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,133:1 +C 5.8% coding (199/621 nt) fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1331.C 5.8% 501.5 / 23.5 121coding (199/621 nt)fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base . (60/54);  new base C (2/5);  total (62/59)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 121 total aligned reads displayed.

GGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGG  >  NC_000913/4428986‑4429275
                                                                                                                                                     |                                                                                                                                              
ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAATTGCTCCTGCGCGAAGAACTGGTTGCAGGTATTGCGGATGCTCTGGAAGGCAAAAATCAGGCTGATAAGGTTGATGTGGTGCATCGCGCTCTGCGAGAAATCCACGAg                                                                                                                                              >  1:238891/1‑150 (MQ=255)
ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                              >  1:939050/1‑150 (MQ=255)
ggTGTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCGGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                              <  1:976236/150‑1 (MQ=255)
  gttGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcg                                                                                                                                            <  1:1298192/149‑1 (MQ=255)
       aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcgcgcga                                                                                                                                       <  1:735538/150‑3 (MQ=255)
       aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcgcg                                                                                                                                        <  2:907940/150‑2 (MQ=255)
         gTAGGGCAACAACTGTGTGAATCTGGCCTGTACGGGCTGCTGCCAGAATCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                                     <  1:1131021/150‑1 (MQ=255)
         gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                                     <  1:625323/150‑1 (MQ=255)
          tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATg                                                                                                                                    >  1:96521/1‑150 (MQ=255)
          gaGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCTGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATg                                                                                                                                    <  2:147649/149‑1 (MQ=255)
           aGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGc                                                                                                                                   <  1:210951/150‑1 (MQ=38)
           aGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGc                                                                                                                                   <  1:1509801/150‑1 (MQ=38)
            gggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGCTGcc                                                                                                                                  >  1:196483/1‑150 (MQ=255)
             ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCg                                                                                                                                 >  1:1238832/1‑150 (MQ=255)
                aacaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGcaccac                                                                                                                              <  2:1698508/150‑6 (MQ=37)
                 acaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcg                                                                                                                             >  2:789893/1‑150 (MQ=255)
                 acaacTGAGGGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTGCCGGTTGATGTGGTGCATCGCGCGCTTCGTGAAATCCACGAGCGCGCCGATGCCGTtcg                                                                                                                             <  1:1274358/150‑1 (MQ=255)
                    acTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                          <  1:490072/150‑1 (MQ=255)
                    acTGAGTGAAGCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                          <  2:1483902/150‑1 (MQ=255)
                      tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtc                                                                                                                        >  1:183299/1‑150 (MQ=255)
                      tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtc                                                                                                                        >  1:426520/1‑150 (MQ=255)
                         gTGAATCTGGCCTGGAAGTGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGc                                                                                                                     >  2:215912/1‑150 (MQ=255)
                          tGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCg                                                                                                                    <  2:685726/150‑1 (MQ=255)
                             aTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGttt                                                                                                                 >  2:892125/1‑150 (MQ=255)
                               cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                               >  2:246885/1‑150 (MQ=255)
                               cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                               <  2:553527/150‑1 (MQ=255)
                                  gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            >  2:1686936/1‑150 (MQ=255)
                                  gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGGTCGTCGTCAGCGTTTCCAgg                                                                                                            >  1:167621/1‑150 (MQ=255)
                                    cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                          <  2:804448/150‑1 (MQ=255)
                                    cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                          >  2:116619/1‑150 (MQ=255)
                                       gAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGAGGCGCTGGAAGGCAAACATCCGGCAGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGTAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATg                                                                                                       <  1:1503727/150‑1 (MQ=255)
                                         aGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                                     >  1:1276797/1‑150 (MQ=255)
                                           ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAATGCAAACATACGGCTGTACCCGTTGATGAGGTGCATCCCGCCCTGCGTGAAATCCACGAGCGCGCCGATCCCGTACTTCGTCAGCGTGTCCAGGCGATGgcag                                                                                                   >  2:672493/1‑148 (MQ=255)
                                           ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                                   >  1:61162/1‑150 (MQ=255)
                                            gctgctGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgc                                                                                                  <  1:1726774/150‑1 (MQ=255)
                                             ctgctgCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgct                                                                                                 <  1:328087/150‑1 (MQ=255)
                                             ctgctgCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGATTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgct                                                                                                 <  2:387379/150‑1 (MQ=255)
                                               gctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGa                                                                                               >  1:1731038/1‑150 (MQ=255)
                                                  gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                            >  2:899083/1‑150 (MQ=255)
                                                  gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                            <  2:613703/150‑1 (MQ=255)
                                                    cAGAAGCTCTGGTGGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                          <  2:481774/150‑1 (MQ=255)
                                                     aGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtgt                                                                                         >  1:1222075/1‑150 (MQ=255)
                                                        aGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGaa                                                                                      <  2:100690/150‑1 (MQ=255)
                                                         gCACTGGTTGCAGGTTTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGaaa                                                                                     <  1:357602/150‑1 (MQ=255)
                                                          cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGAGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                    <  2:279445/150‑1 (MQ=255)
                                                           aCTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCAATGGCTGCTGAAGGTGTGAAATa                                                                                   >  1:370623/1‑150 (MQ=255)
                                                              ggTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCt                                                                                >  1:1243405/1‑150 (MQ=255)
                                                               gTTGCAGGTATTGCCGATGCGCTGGCAGGCAAACATCCGGCTGTTCCGGTGGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                               <  1:266429/150‑1 (MQ=255)
                                                                ttGCAGGTATTGCCGTTGCGCTGGAAGGCATACATCCGGCTGTGCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTgg                                                                              <  2:939050/150‑1 (MQ=255)
                                                                   cAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                           >  1:1025160/1‑150 (MQ=255)
                                                                     ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            >  1:1251258/1‑115 (MQ=255)
                                                                     ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            <  2:1251258/115‑1 (MQ=255)
                                                                     ggTAGTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaagaa                                                                         <  1:56367/150‑1 (MQ=255)
                                                                         ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACg                                                                     <  1:1590548/150‑1 (MQ=255)
                                                                           gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                                   >  1:1664486/1‑150 (MQ=255)
                                                                              gATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaa                                                                >  2:1195070/1‑150 (MQ=255)
                                                                                tGCGCTGGAATGCAAACATCCGGCTGTTCCGGTTGATGTTGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaaaa                                                              <  2:387590/150‑1 (MQ=255)
                                                                                  cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                            >  1:24778/1‑150 (MQ=255)
                                                                                   gcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaaga                                                           >  2:748435/1‑150 (MQ=255)
                                                                                     tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                         <  2:1042164/150‑1 (MQ=255)
                                                                                     tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                         <  1:246885/150‑1 (MQ=255)
                                                                                      ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                        <  1:708023/150‑1 (MQ=255)
                                                                                          ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                    >  1:1673940/1‑150 (MQ=255)
                                                                                          ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATCCCGTTCGTCGTCAGCGTTTCCATGCGATGGCTGCTGAAGGGGTGAACTACCTCCAAGCAAACCCCAACCACGCAGGtgtg                                                    >  1:301213/1‑150 (MQ=255)
                                                                                            cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                  <  1:1686936/150‑1 (MQ=255)
                                                                                            cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                  >  2:427288/1‑150 (MQ=255)
                                                                                             aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAAt                                                 >  1:618551/1‑150 (MQ=255)
                                                                                               aCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAg                                               >  1:427764/1‑150 (MQ=255)
                                                                                                    cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGTTGGCTGCTGAAGGTGTGAATTACCGGGAAGAAAACGCCAAAACAGAAGGTGGGCTTAGCCCCg                                          >  1:1136541/1‑150 (MQ=255)
                                                                                                    cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCg                                          <  1:1113529/150‑1 (MQ=255)
                                                                                                     ggaacagccGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                         >  2:218932/8‑150 (MQ=255)
                                                                                                     ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                         >  1:1129311/1‑150 (MQ=255)
                                                                                                     ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                         >  2:669456/1‑150 (MQ=255)
                                                                                                         gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCTCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATCGCACCGAATCt                                     >  2:1639460/1‑150 (MQ=255)
                                                                                                         gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCt                                     <  1:1347366/150‑1 (MQ=255)
                                                                                                         gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCt                                     <  1:1483105/150‑1 (MQ=255)
                                                                                                          gtCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCAGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                    >  1:632118/2‑150 (MQ=255)
                                                                                                             cGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                                 <  1:496225/150‑1 (MQ=255)
                                                                                                               gTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGGCAGCGTTTCCAGGCGAGGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTg                               <  1:915435/150‑1 (MQ=255)
                                                                                                                ttGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGc                              <  1:806836/150‑1 (MQ=255)
                                                                                                                 tGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGCGCGCGGCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCa                             >  1:695063/1‑150 (MQ=255)
                                                                                                                  gATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCaa                            >  2:486667/1‑150 (MQ=255)
                                                                                                                   aTGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAAACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTCGCCTGCAAt                           >  2:467315/1‑150 (MQ=255)
                                                                                                                       ggtgCATCGCGCGCTGAGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCATAAAAGAAGGTGTTAATAGCACCGATTCTGGCCTGCAATTCcg                       <  1:368679/150‑1 (MQ=255)
                                                                                                                        gtgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgc                      >  2:1239503/1‑150 (MQ=255)
                                                                                                                          gCATCGCGCGCTGCGTGAAAACCACGAGCGCGCCGATGCCGTTCGGCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGAGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                    <  1:599487/150‑1 (MQ=255)
                                                                                                                           catcggcgcgcTCGTGGATTTCACGCAGCGCGCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg                   >  1:246269/6‑150 (MQ=38)
                                                                                                                            aTCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa                  >  2:326447/1‑150 (MQ=255)
                                                                                                                             tCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                 <  2:773501/150‑1 (MQ=255)
                                                                                                                                gcgcgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCa               >  2:421023/1‑150 (MQ=255)
                                                                                                                                   cgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAcc            <  1:700409/150‑1 (MQ=255)
                                                                                                                                    gcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCa           >  1:1454549/1‑150 (MQ=255)
                                                                                                                                     cTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg          <  2:1731038/150‑1 (MQ=255)
                                                                                                                                       gagTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCTGGCGATGGCTGAGGCAGGTGTGAAATACCTGGAAGACAACGCCAAAAAAGCAGGTGTGAATAGGAACGGATCTGGCCGGCCAGGCCGCGTGCGCATCCAggg        >  2:836438/3‑150 (MQ=255)
                                                                                                                                        ggTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTTAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAAGGt       <  1:116619/149‑1 (MQ=255)
                                                                                                                                         gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg      >  1:814802/1‑150 (MQ=255)
                                                                                                                                         gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGAGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg      >  2:1116009/1‑150 (MQ=255)
                                                                                                                                         gTGAAATCCACGAGCGCGCCGAGGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg      <  1:1165511/150‑1 (MQ=255)
                                                                                                                                             aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg  <  2:167621/150‑1 (MQ=255)
                                                                                                                                             aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTGCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCTATTCCGCGTGATCAACCAGGGTGAAgg  <  1:899083/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGG  >  NC_000913/4428986‑4429275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: