Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,299,267:1 +A 5.8% intergenic (+22/‑1915) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,299,2671.A 5.8% 469.5 / 21.7 120intergenic (+22/‑1915)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (67/46);  new base A (5/2);  total (72/48)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 122 total aligned reads displayed.

TGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCG  >  NC_000913/1299118‑1299416
                                                                                                                                                      |                                                                                                                                                     
    aCGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGcc                                                                                                                                                   >  1:350167/1‑150 (MQ=255)
     cGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCa                                                                                                                                                  >  2:487776/1‑150 (MQ=255)
        gTTTTACGCCGGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGc                                                                                                                                               >  1:66161/1‑150 (MQ=255)
        gTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTTATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGc                                                                                                                                               >  2:1548020/1‑150 (MQ=255)
          tttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTc                                                                                                                                             <  1:161261/150‑1 (MQ=255)
             aCGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGt                                                                                                                                          <  2:789155/150‑1 (MQ=255)
              cGCCATACCGGCATCAAAGTGATTACGCGTATTATGGGGCTATTGCTGATGGAATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGtt                                                                                                                                         <  1:205985/150‑1 (MQ=255)
              cGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGATGCCATGCTCCGtt                                                                                                                                         >  1:131427/1‑150 (MQ=255)
              cGCCAGACCGGCATCAACGTGATTAAGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAAATTATCGTTACTGGTATTAAGGGGATTTTCCC‑‑‑ACTGATTAATTAATACCATTCAAATGAAACGGAGCTGCCATGCTCCGTTTaa                                                                                                                                      >  1:408093/1‑149 (MQ=255)
               gCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGttt                                                                                                                                        >  1:677066/1‑150 (MQ=255)
               gCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGttt                                                                                                                                        >  1:1627742/1‑150 (MQ=255)
               gCCAGACCGGCATAATCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCTTTGGGTATTGAATTTATCGTTACTGATATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGttt                                                                                                                                        <  2:1549693/150‑1 (MQ=255)
                ccAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTa                                                                                                                                       <  2:665995/150‑1 (MQ=255)
                 cAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtttca                                                                                                                                      <  1:1348532/150‑3 (MQ=255)
                     ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTTCTTCg                                                                                                                                  >  1:780792/1‑150 (MQ=255)
                     ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCg                                                                                                                                  <  1:446023/150‑1 (MQ=255)
                            cAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattat                                                                                                                           >  1:996482/1‑150 (MQ=255)
                            cAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattat                                                                                                                           >  2:1612563/1‑150 (MQ=255)
                             aaCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattatt                                                                                                                          >  1:553283/1‑150 (MQ=255)
                              aCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTAttt                                                                                                                         >  1:1180762/1‑150 (MQ=255)
                               cGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTAtttt                                                                                                                        <  2:136883/150‑1 (MQ=255)
                                 tGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTa                                                                                                                      >  2:1426047/1‑150 (MQ=255)
                                        gcttattatGTGGATATTTATGATGGAATTGGGGATTGAATTTATAGTTACTGGTATTAAGGGGATTTTCACCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGtt                                                                                                               <  2:1142682/147‑1 (MQ=255)
                                         cgTATTATTGGGCTATTGCTGATGGCATTGGTGATTGAATTTCTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTAAATTAATTCCTTTCAAATGAAACTGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTc                                                                                                              <  1:266802/150‑1 (MQ=255)
                                         cgTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTc                                                                                                              >  2:1562629/1‑150 (MQ=255)
                                          gTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTcc                                                                                                             >  1:1377522/1‑150 (MQ=255)
                                               atGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTTTTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCgcgc                                                                                                        <  2:1594916/150‑1 (MQ=255)
                                                tGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCa                                                                                                       >  2:476809/1‑150 (MQ=255)
                                                tGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCa                                                                                                       >  2:1475346/1‑150 (MQ=255)
                                                 ggggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAg                                                                                                      <  2:39421/150‑1 (MQ=255)
                                                  gggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGt                                                                                                     >  2:401837/1‑150 (MQ=255)
                                                       aTTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATca                                                                                                <  2:553283/150‑1 (MQ=255)
                                                          gCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaa                                                                                             >  1:158281/1‑150 (MQ=255)
                                                           cTGATGGCATTGTGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaag                                                                                            <  1:376856/150‑1 (MQ=255)
                                                               tGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaa                                                                                        <  1:945472/150‑1 (MQ=255)
                                                               tGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaa                                                                                        <  2:1428110/150‑1 (MQ=255)
                                                                ggCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaaa                                                                                       <  1:546359/150‑1 (MQ=255)
                                                                      ggggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATa                                                                                 <  1:48371/150‑1 (MQ=255)
                                                                      ggggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATa                                                                                 <  1:1424958/150‑1 (MQ=255)
                                                                         gATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGt                                                                              >  2:500627/1‑150 (MQ=255)
                                                                          aTTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTa                                                                             <  1:1134859/150‑1 (MQ=255)
                                                                             gAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTaaaa                                                                          >  1:958281/1‑150 (MQ=255)
                                                                                  tATCGTTACTGGTATTAAGGTGATTTTCCCCGGACTGCTTATTTAATTCCTTTCTAATGAAACGGAGCTGCCATTCTCCGTTTACTTCTTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAACGGAATAGGTAAAAATAtt                                                                     <  1:46169/150‑1 (MQ=255)
                                                                                   aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTTTCAAAAGCAAAAGGAATAGGTAAAAATAttc                                                                    >  2:841287/1‑150 (MQ=255)
                                                                                   aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaaag                                                                                      <  1:20536/132‑1 (MQ=255)
                                                                                   aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaaag                                                                                      >  2:20536/1‑132 (MQ=255)
                                                                                     cGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTAGTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttctt                                                                  >  2:276734/1‑150 (MQ=255)
                                                                                      gTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttcttc                                                                 >  2:559282/1‑150 (MQ=255)
                                                                                      gTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttcttc                                                                 <  2:1269959/150‑1 (MQ=255)
                                                                                         aCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCa                                                              <  2:1627742/150‑1 (MQ=255)
                                                                                         aCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCa                                                              <  2:677066/150‑1 (MQ=255)
                                                                                            ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                           >  1:537711/1‑150 (MQ=255)
                                                                                            ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                           >  1:504570/1‑150 (MQ=255)
                                                                                                 tAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACag                                                      <  2:1507064/150‑1 (MQ=255)
                                                                                                  aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACTGAGGTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAACAGCTAAAGGAATAGGTTAAAATATTCTTCTCAAATTActct                                                     >  2:265165/1‑147 (MQ=255)
                                                                                                  aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagt                                                     >  2:1244116/1‑150 (MQ=255)
                                                                                                  aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGAGAAGTTATAAAAAACAAAAGAAATAGTTAAATAAATTATTATAAAATTACatt                                                     >  1:293307/1‑148 (MQ=255)
                                                                                                   aGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtt                                                    <  2:904353/150‑1 (MQ=255)
                                                                                                    ggggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtta                                                   >  2:738381/1‑150 (MQ=255)
                                                                                                     gggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTGATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttat                                                  >  1:122586/1‑149 (MQ=255)
                                                                                                       gATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttagtt                                                >  1:307354/1‑150 (MQ=255)
                                                                                                       gATTTTCCCCGGCCTGCTTAATTAAATCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCAATATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAAAAGGTAAAAATATTCTTCTCAAATTACagttagtt                                                >  2:315072/1‑150 (MQ=255)
                                                                                                        aTTTTCCCCGGCCTGCTTAATTACTTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAgttagtta                                               <  2:183671/150‑1 (MQ=255)
                                                                                                        aTTTTCACCGGCATGATTAATTAATTCCTTTCATATGATACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAGGTAATAGGTAAAAATATTCTTCTCAAATTACAgttagtta                                               <  2:133705/150‑1 (MQ=255)
                                                                                                          tttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAAAGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTtata                                             <  1:1051657/150‑1 (MQ=255)
                                                                                                           ttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATaa                                            >  2:163015/1‑150 (MQ=255)
                                                                                                            tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                           <  1:1475346/150‑1 (MQ=255)
                                                                                                            tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                           <  1:466629/150‑1 (MQ=255)
                                                                                                            tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                           >  1:870502/1‑150 (MQ=255)
                                                                                                            tCCCCGGCCTGCTTAATTAATTACTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                           <  2:1172116/150‑1 (MQ=255)
                                                                                                             ccccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGAAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAgg                                          >  2:1692664/1‑150 (MQ=255)
                                                                                                              cccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGa                                         >  1:488461/1‑150 (MQ=255)
                                                                                                               ccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGAt                                        >  2:1414904/1‑150 (MQ=255)
                                                                                                                    cTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGAAATCCt                                   <  1:1089080/150‑1 (MQ=255)
                                                                                                                          aattaattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTg                             <  2:1099811/150‑1 (MQ=255)
                                                                                                                          aattaattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTg                             <  1:1548020/150‑1 (MQ=255)
                                                                                                                             taattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCtt                          >  1:610221/1‑150 (MQ=255)
                                                                                                                               attCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtct                        >  2:860524/1‑150 (MQ=255)
                                                                                                                                  ccTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTATAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcct                     >  1:305027/1‑150 (MQ=255)
                                                                                                                                  ccTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAACTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcct                     >  1:338962/1‑150 (MQ=255)
                                                                                                                                  ccTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcct                     <  2:1355260/150‑1 (MQ=255)
                                                                                                                                   cTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcctc                    >  1:598336/1‑150 (MQ=255)
                                                                                                                                    tttCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCa                   <  1:163015/150‑1 (MQ=255)
                                                                                                                                       cAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACca                >  2:383301/1‑150 (MQ=255)
                                                                                                                                       cAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACca                <  1:100763/150‑1 (MQ=255)
                                                                                                                                        aaaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcat               >  1:588062/1‑150 (MQ=255)
                                                                                                                                        aaaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcat               >  2:113796/1‑150 (MQ=255)
                                                                                                                                        aaaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcat               >  1:811545/1‑150 (MQ=255)
                                                                                                                                         aaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatc              >  1:596552/1‑150 (MQ=255)
                                                                                                                                          aTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca             >  1:357027/1‑150 (MQ=255)
                                                                                                                                          aTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTATGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCATTAACTGCTTCTCATCACcatca             >  2:1525771/1‑150 (MQ=255)
                                                                                                                                            taaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg           >  2:678468/2‑150 (MQ=255)
                                                                                                                                            gAAACGGAGCTGCCATTCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg           <  2:771572/150‑1 (MQ=255)
                                                                                                                                            gAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg           >  2:740423/1‑150 (MQ=255)
                                                                                                                                             aaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt          >  2:622685/1‑150 (MQ=255)
                                                                                                                                             aaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt          >  2:907233/1‑150 (MQ=255)
                                                                                                                                             aaaCGGAGCATGGCAGCTCCGTTTACTTCCTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTATTCTCAAATTACAGTTAGTTATAAGGATATCCTTAAATGCTTATCCTCACCATCATGt          >  1:66151/1‑150 (MQ=255)
                                                                                                                                              aaCGGAGCTGACATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTACTTAACTGCTTCTCCTCACCATCATGtt         >  1:94363/1‑150 (MQ=255)
                                                                                                                                                    gCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTAtttt  >  1:1449029/1‑149 (MQ=255)
                                                                                                                                                     cTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCg  >  2:570963/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCG  >  NC_000913/1299118‑1299416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: