Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,524,458:1 +T 5.1% intergenic (+90/+23) yncE → / ← ansP ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,524,4581.T 5.1% 463.3 / 18.0 117intergenic (+90/+23)yncE/ansPATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base . (52/59);  new base T (2/4);  total (54/63)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TCCACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCA  >  NC_000913/1524312‑1524602
                                                                                                                                                   |                                                                                                                                                
tCCACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACg                                                                                                                                               >  1:410286/1‑150 (MQ=255)
accACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACg                                                                                                                                               <  1:1456961/149‑1 (MQ=255)
   aCTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAg                                                                                                                                            >  1:1535752/1‑150 (MQ=255)
      aaaCAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCg                                                                                                                                         >  2:341440/1‑150 (MQ=255)
        aCAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                                                                       <  1:1014126/150‑1 (MQ=255)
          agcagGAAGCTACCCAGCCAGACGCTGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCa                                                                                                                                     <  1:469415/150‑1 (MQ=255)
           cccggAAGCTCCCCAGCCAGACGCGGTGAGTCGTCTTGCGCGGTACTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaa                                                                                                                                    <  2:300787/147‑1 (MQ=255)
              ggAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCAAatt                                                                                                                                 <  1:677604/150‑1 (MQ=255)
                 aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGc                                                                                                                              >  1:294332/1‑150 (MQ=255)
                  gCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCa                                                                                                                             >  2:225436/1‑150 (MQ=255)
                   cTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                                                                            <  1:369771/150‑1 (MQ=255)
                      cccAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGTGCAATTTTGCAATTa                                                                                                                         >  1:1236171/1‑150 (MQ=255)
                           ccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGGCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCg                                                                                                                    <  1:818358/150‑1 (MQ=255)
                               ccGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGt                                                                                                                <  1:941301/149‑1 (MQ=255)
                               aCGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGt                                                                                                                <  2:1236171/150‑1 (MQ=255)
                                 gATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGttt                                                                                                              >  1:548764/1‑150 (MQ=255)
                                  aTGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGGGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc                                                                                                             <  1:485778/150‑1 (MQ=255)
                                    gtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAg                                                                                                           >  1:1492116/1‑150 (MQ=255)
                                      gATTCGGATTGCGCTGTAATAGAAATGGCGAGGGGGCAAACGGGGAGACTTTCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAAGCTGACTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                                                                         <  2:355639/150‑1 (MQ=255)
                                       aTTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCt                                                                                                        <  2:1421457/150‑1 (MQ=255)
                                          cggaTTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaa                                                                                                     <  1:876474/147‑1 (MQ=255)
                                          cGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaa                                                                                                     >  2:1080507/1‑150 (MQ=255)
                                           gTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaa                                                                                                    >  1:998100/1‑150 (MQ=255)
                                            tATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAac                                                                                                   >  1:606679/1‑150 (MQ=255)
                                             gttGCGCTGTAATAGATATGGCGAGGGTGAAAAGGGTGAGCCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTGGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaccc                                                                                                  >  1:482794/2‑147 (MQ=255)
                                             aTTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAaca                                                                                                  >  1:482790/1‑150 (MQ=255)
                                               tGCGCTGTAATAGATATGGCGAGGGTTAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAAcaca                                                                                                <  1:1648564/150‑1 (MQ=255)
                                                  gcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                                                             <  2:1411720/150‑1 (MQ=255)
                                                  gcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                                                             <  2:699436/150‑1 (MQ=255)
                                                   cTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGAAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAttt                                                                                            >  1:936330/1‑150 (MQ=255)
                                                    tGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTc                                                                                           <  1:1636609/150‑1 (MQ=255)
                                                     gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                                                          >  1:247568/1‑150 (MQ=255)
                                                     gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                                                          <  2:1359316/150‑1 (MQ=255)
                                                       aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCTTCAGTAATCTGAATTTTCCCGGCACGTAGACGGGCAATTTAGCAATTAACTCGCCGTTTCAGGCTTAAAAACAATTTCCtt                                                                                        >  2:1161966/1‑149 (MQ=255)
                                                       aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCTGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg                                                                                        >  2:1155937/1‑150 (MQ=255)
                                                       aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg                                                                                        <  2:1185422/150‑1 (MQ=255)
                                                       aaTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg                                                                                        >  1:638182/1‑150 (MQ=255)
                                                         tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtt                                                                                      <  2:960146/150‑1 (MQ=255)
                                                          aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttt                                                                                     >  2:401922/1‑150 (MQ=255)
                                                           gATATGGGGAGGGTGAAAACGGTGAGACTTGCCGGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTGGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                                                                    <  2:482794/150‑1 (MQ=255)
                                                           gATATGGCGAGGGTGAAAACGGTGATACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCCGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                                                                    <  2:1228973/150‑1 (MQ=255)
                                                           gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                                                                    <  2:482790/150‑1 (MQ=255)
                                                            atctgccGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGTCGTATCCGGCACTTTAGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGTGCAATTTTGCAATTAACTCGCCCTTTCAGGCTTAAACACAATTTCCTGttttt                                                                                   <  1:111667/144‑1 (MQ=255)
                                                              atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                                                                 >  2:1201173/1‑150 (MQ=255)
                                                                ggCGCGGGTGAAACCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                                                               <  2:1550823/150‑1 (MQ=255)
                                                                ggCGAGGGTGAAAACGGTTAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGGTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                                                               <  2:493107/150‑1 (MQ=255)
                                                                ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                                                               >  2:953521/1‑150 (MQ=255)
                                                                 gcgctgctGAAAACGGTCAGACTAGCCTGCCTTTTCTCACGGCGTAGCCGGCCCTTGCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTGCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                                                              <  2:169112/143‑1 (MQ=255)
                                                                 gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                                                              >  2:615376/1‑150 (MQ=255)
                                                                     gggTGAAAACGGTGAGACTTTCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCATTAATCTGAATTTGCACGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCtt                                                                          <  2:1082251/150‑1 (MQ=255)
                                                                       gTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTcc                                                                        <  1:554312/150‑1 (MQ=255)
                                                                        tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt                                                                       >  1:1267144/1‑150 (MQ=255)
                                                                        tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt                                                                       >  1:1226925/1‑150 (MQ=255)
                                                                         gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTc                                                                      >  2:1508455/1‑150 (MQ=255)
                                                                         gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGACACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTc                                                                      <  1:1244025/150‑1 (MQ=255)
                                                                          aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                                                                     <  2:576134/150‑1 (MQ=255)
                                                                          aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCAGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                                                                     >  1:1500780/1‑150 (MQ=255)
                                                                            aaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGTCACTTTCGACAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATATTGCAATTAACTCGCCGTTTCAGGCTTAATCACAGTTTCCTGTTTTTCTTCATCTTCCTcgac                                                                   >  1:277602/1‑150 (MQ=255)
                                                                             aCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                                                                  >  2:442343/1‑150 (MQ=255)
                                                                             aCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                                                                  <  2:669924/150‑1 (MQ=255)
                                                                               ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCgacgac                                                                >  1:1419395/1‑150 (MQ=255)
                                                                               ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCgacgac                                                                <  2:247568/150‑1 (MQ=255)
                                                                                gTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                                                               <  2:829127/150‑1 (MQ=255)
                                                                                gTGAGACTTGCCTGCATTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                                                               >  2:872101/1‑150 (MQ=255)
                                                                                 tGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                                                              <  2:1706648/150‑1 (MQ=255)
                                                                                   agaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc                                                            <  2:1514677/150‑1 (MQ=255)
                                                                                        tgcctgccTTTTATCACGGCGTATCAGGCACTTTCGTCAGTTATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTATTCATCTTCCTCTACGACTTGCGCAGt                                                       >  1:1558343/1‑150 (MQ=255)
                                                                                             gcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgt                                                  <  2:548764/150‑1 (MQ=255)
                                                                                             gcctTTTATCACGGCGTATCCGGCACTTTCGGCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgt                                                  <  2:1048924/150‑1 (MQ=255)
                                                                                              cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgtg                                                 <  2:1535752/150‑1 (MQ=255)
                                                                                               ctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAATAACTCGCCGTTTCAGGCTTAAACACAAATTCCTGTTTTTCTTCATAATCATCGACGACTGGCGCAGTGCTgaga                                                >  1:1044067/1‑150 (MQ=255)
                                                                                                ttttATCACGGCGGTTCCGGCACTTTCGTAAGTAAGCTGAATTTGCCCGGCACGTAGCCGTGAAATTTTTAAATTAACACGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGGGCTGTGaa                                               <  2:281754/150‑1 (MQ=255)
                                                                                                      cACGGCGTATCCGTCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg                                         <  1:1020241/150‑1 (MQ=255)
                                                                                                      cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg                                         >  1:898436/1‑150 (MQ=255)
                                                                                                      cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACCACTGGCGCAGTGCTGTGAATTTCAg                                         >  2:505253/1‑150 (MQ=255)
                                                                                                       aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        <  1:953521/150‑1 (MQ=255)
                                                                                                           cGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcg                                    <  1:1290925/150‑1 (MQ=255)
                                                                                                           cGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcg                                    <  1:1455815/150‑1 (MQ=255)
                                                                                                               tCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACTTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGtt                                <  1:640161/150‑1 (MQ=255)
                                                                                                                 cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        >  1:1152278/1‑140 (MQ=255)
                                                                                                                 cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGc                                        <  2:1152278/140‑1 (MQ=255)
                                                                                                                   gCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACACGCCGTTTCAGGCTTAAACACATTTTCCAGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTAATCAACGCGTTTgcg                            >  1:352532/1‑150 (MQ=255)
                                                                                                                      cTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcac                         >  2:204333/1‑150 (MQ=255)
                                                                                                                       tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcaca                        >  2:1184057/1‑150 (MQ=255)
                                                                                                                       tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcaca                        <  2:410286/150‑1 (MQ=255)
                                                                                                                                  aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc             >  1:1508214/1‑150 (MQ=255)
                                                                                                                                  aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc             >  1:648228/1‑150 (MQ=255)
                                                                                                                                   tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc                 >  2:1550525/1‑145 (MQ=255)
                                                                                                                                   tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAAcc                 <  1:1550525/145‑1 (MQ=255)
                                                                                                                                   tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACAGGCGAAGTGCTGTGAATTTCAGCAACGCGTTAGCGCACACCAAACCAGcca            >  2:883309/1‑149 (MQ=255)
                                                                                                                                     caaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCtat          >  2:903381/6‑150 (MQ=255)
                                                                                                                                      gAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCtata         <  2:1267144/150‑1 (MQ=255)
                                                                                                                                        aTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAc       <  1:718605/150‑1 (MQ=255)
                                                                                                                                         tttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAcc      <  1:1657690/150‑1 (MQ=255)
                                                                                                                                         tttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAcc      >  2:1526089/1‑150 (MQ=255)
                                                                                                                                         attGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCTCACACCAAACCAGCCTATAAcc      >  1:1138507/2‑150 (MQ=255)
                                                                                                                                          ttGCCCGGCACGTAGCCGGGCAATTTTGCAATTACCTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGGGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCa     <  2:1079334/150‑1 (MQ=255)
                                                                                                                                          ttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCATCCAAAAACCa     >  2:813234/1‑150 (MQ=255)
                                                                                                                                           tGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAg    <  2:240952/150‑1 (MQ=255)
                                                                                                                                             cccGGCACGTAGCCTGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCa  <  1:1252827/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCCACTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCA  >  NC_000913/1524312‑1524602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: