Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,625,312:1 +G 6.9% intergenic (+21/+23) ydeJ → / ← dcp inactive PncC family protein/dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,625,3121.G 6.9% 404.6 / 24.5 101intergenic (+21/+23)ydeJ/dcpinactive PncC family protein/dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base . (45/49);  new base G (3/4);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01

Only 100 of 103 total aligned reads displayed.

GTCTGGTTTGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAAC  >  NC_000913/1625163‑1625456
                                                                                                                                                      |                                                                                                                                                 
gTCTGGTTTGCGTGGCATATTAAAGGCCAGACCTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCATTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATAtc                                                                                                                                                    >  1:1448789/1‑150 (MQ=255)
gTCTGGTTTGCGGGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATAtc                                                                                                                                                    <  2:1207007/150‑1 (MQ=255)
   tGGTTTGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTCGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCa                                                                                                                                               <  1:1311822/150‑1 (MQ=255)
    ggTTTGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAg                                                                                                                                              >  2:1019796/1‑150 (MQ=255)
    ggTTTGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAg                                                                                                                                              >  2:1165453/1‑150 (MQ=255)
    ggTTTGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAg                                                                                                                                              >  1:193864/1‑150 (MQ=255)
      tttGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGgc                                                                                                                                            <  2:810613/150‑1 (MQ=255)
         gCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGACCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGAGATATCGccaggccag                                                                                                                                         <  1:122446/150‑1 (MQ=255)
           gtttcATATATAAGGCCAGATCTACACTGCGGTTATGCATTTTGCTGGCGACTGCGATACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGAGATATCGCCAGgccagcc                                                                                                                                       <  1:146488/146‑1 (MQ=255)
           gTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGATACTGTATTATCTTTAGCGGTGAGGTTTGCACTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGgccagcc                                                                                                                                       <  2:857120/150‑1 (MQ=255)
           gTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGAGATATCGCCAGgccagcc                                                                                                                                       <  1:316056/150‑1 (MQ=255)
            tGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGgccagcca                                                                                                                                      >  1:1337006/1‑150 (MQ=255)
            tGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGAGATATCGCCAGgccagcct                                                                                                                                      <  1:1634317/150‑2 (MQ=255)
             ggCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCAt                                                                                                                                     >  1:201307/1‑150 (MQ=255)
                atatTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATtgg                                                                                                                                  >  1:1525464/1‑150 (MQ=255)
                atatTAAAGGCCAGAACTACACTGCGGGTAGGCATTTTGCTGCCGACTGCGAAACGGTCTTAGCTTTAGCGGCGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATtgg                                                                                                                                  <  2:1638979/150‑1 (MQ=255)
                  atTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATtggtg                                                                                                                                <  2:1182006/150‑1 (MQ=255)
                   ttAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATtggtgg                                                                                                                               <  2:1064100/150‑1 (MQ=255)
                     aaaGGCCAGAACTACACTGCGGTTATGCATTTTGCTGTCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCGGCTGCAATTACTGCTATATCACGGATGGCCTGGCGATATCTCAGGCCAGCCATTGgtggtg                                                                                                                             >  2:73358/1‑150 (MQ=255)
                     aaaGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGTCTGGCCTGGCGATATCTCAGGCCAGCCATTggtggtt                                                                                                                             >  2:1579414/1‑149 (MQ=255)
                     aaaGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGgtggtg                                                                                                                             >  2:1411348/1‑150 (MQ=255)
                      aaGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGTGTTTTTTTTTTATCTCGCCGAGCTGATGGATTTATTGATATAAAATGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGgtgt                                                                                                                            <  2:445778/150‑1 (MQ=255)
                       aGGCCAGAACTACACTGCGGTTATGCATGTTGCTGGCGACTGCGAAACGGTATTAGCGTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCGGCAATTACTGCTATAACCAGGCGGGCCTGGCGATATCTCCGGCCAGCCATTGGTGGTGtt                                                                                                                           <  2:1624408/150‑1 (MQ=255)
                        ggCCAGATCTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGttt                                                                                                                          <  2:1162863/150‑1 (MQ=255)
                         gCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTta                                                                                                                         >  2:1546687/1‑150 (MQ=255)
                          ccAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGGGTTtat                                                                                                                        >  2:847168/1‑150 (MQ=255)
                             gatcTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATg                                                                                                                     <  1:958219/147‑1 (MQ=255)
                                cTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGtta                                                                                                                  >  1:996121/1‑149 (MQ=255)
                                cTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTc                                                                                                                  <  2:1514993/150‑1 (MQ=255)
                                cTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTc                                                                                                                  >  2:504577/1‑150 (MQ=255)
                                 tACACTTCGGTTATGCATTTTGCGGGCGACTGCGCAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCACAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCa                                                                                                                 <  2:917933/150‑1 (MQ=255)
                                  acacTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGGGTTTATATGTTCaa                                                                                                                >  1:830776/1‑150 (MQ=255)
                                     cTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGcc                                                                                                             <  1:945039/150‑1 (MQ=255)
                                     cTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGcc                                                                                                             >  1:170404/1‑150 (MQ=255)
                                        cGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGGGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACg                                                                                                          <  1:64208/150‑1 (MQ=255)
                                               gCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTgc                                                                                                   >  2:1612769/1‑150 (MQ=255)
                                               gCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTgc                                                                                                   >  1:668348/1‑150 (MQ=255)
                                                 aTTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTgcag                                                                                                 <  1:1451628/150‑1 (MQ=255)
                                                      gCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCg                                                                                            >  2:862442/1‑150 (MQ=255)
                                                      gCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCg                                                                                            >  1:1304183/1‑150 (MQ=255)
                                                      gCTGGCGACTGCGAAACGGTATTAGCTTTAACGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCg                                                                                            >  1:659051/1‑150 (MQ=255)
                                                        tGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGc                                                                                          <  1:1655719/150‑1 (MQ=255)
                                                           cGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATa                                                                                       >  2:963569/1‑150 (MQ=255)
                                                           cGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGCTGCAACATCgtggct                                                                                       <  1:339349/150‑6 (MQ=255)
                                                                gCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCtt                                                                                  >  2:1308662/1‑150 (MQ=255)
                                                                 cGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTa                                                                                 <  2:1607677/150‑1 (MQ=255)
                                                                     aCGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTg                                                                             <  2:1337006/150‑1 (MQ=255)
                                                                      cGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGc                                                                            >  1:1075031/1‑150 (MQ=255)
                                                                       ggTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGcc                                                                           <  1:238507/150‑1 (MQ=255)
                                                                       ggTATTAGCTTTAGCGGTGAGGTTTGCCCGCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGcc                                                                           <  2:1066760/150‑1 (MQ=255)
                                                                       ggTATTAGCTTTAGCGGTGAGGCTTGCCCTCGCCCAGCGGCTGCAATTACTGCAATACCCAGGCCGGCCGGGCGATAGCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTAAGGTGcc                                                                           <  2:229211/150‑1 (MQ=255)
                                                                        gTATTAGCTTTAGCGGTGAGGGTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCt                                                                          <  2:831070/150‑1 (MQ=255)
                                                                             aGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCTTTGGTGGTGTTTATATGTTCTAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACcg                                                                     >  2:684378/1‑150 (MQ=255)
                                                                             aGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACcg                                                                     >  2:1155928/1‑150 (MQ=255)
                                                                             aGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACcg                                                                     <  2:326827/150‑1 (MQ=255)
                                                                                 ttAGCGGGGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc                                                                 <  2:387855/150‑1 (MQ=255)
                                                                                   aGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCAt                                                               <  1:1633692/150‑1 (MQ=255)
                                                                                      ggTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCTGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATATTCTTAGGTGCCTTACCGCGCCATTGt                                                            >  1:689734/1‑150 (MQ=255)
                                                                                      ggTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACAGCGCCATTGt                                                            >  1:618006/1‑150 (MQ=255)
                                                                                       gTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTTCTATAACCAGGCTGGCCTGGAGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc                                                           <  2:467617/150‑1 (MQ=255)
                                                                                       gTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCGATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc                                                           <  1:208433/150‑1 (MQ=255)
                                                                                       gTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc                                                           <  2:337181/150‑1 (MQ=255)
                                                                                       gTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc                                                           >  1:1651094/1‑150 (MQ=255)
                                                                                       gTGAGGTTAGCCCTCGCACAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGAAATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTAACCGCGCCATTGTc                                                           <  1:684348/150‑1 (MQ=255)
                                                                                        tGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCg                                                          <  1:567743/150‑1 (MQ=255)
                                                                                          aGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGAt                                                        <  1:640772/150‑1 (MQ=255)
                                                                                           ggTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATa                                                       <  1:924670/150‑1 (MQ=255)
                                                                                                gCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGc                                                  <  1:862442/150‑1 (MQ=255)
                                                                                                   cTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGtt                                               >  1:1522774/1‑150 (MQ=255)
                                                                                                      gCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCa                                            >  1:1143700/1‑150 (MQ=255)
                                                                                                       cccAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTTGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAg                                           <  1:837399/150‑1 (MQ=255)
                                                                                                           gctgctGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCt                                       <  1:1292773/150‑1 (MQ=255)
                                                                                                              gctgcAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCg                                    <  1:1579414/150‑1 (MQ=255)
                                                                                                              gctgcAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCg                                    <  1:1165453/150‑1 (MQ=255)
                                                                                                               ctgcAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGATCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGc                                   <  2:1075031/150‑1 (MQ=255)
                                                                                                                  cAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGt                                <  2:1562023/150‑1 (MQ=255)
                                                                                                                     ttACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTAc                             >  1:1428738/1‑150 (MQ=255)
                                                                                                                       aCTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACct                           <  1:552314/150‑1 (MQ=255)
                                                                                                                            tataACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGa                      >  1:964008/1‑150 (MQ=255)
                                                                                                                            tataACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGa                      >  1:1666893/1‑150 (MQ=255)
                                                                                                                            tataACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGa                      >  1:1673352/1‑150 (MQ=255)
                                                                                                                            tataACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATCTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCTCTGTTACCTCTGGa                      >  2:741243/1‑150 (MQ=255)
                                                                                                                              taACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTTGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGaaa                    <  1:1455404/150‑1 (MQ=255)
                                                                                                                              taACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGaaa                    <  1:1474485/150‑1 (MQ=255)
                                                                                                                                aCCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAATCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg                  <  2:907637/150‑1 (MQ=255)
                                                                                                                                 aatggCTGGCCTGAGATATCGCCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGa                 >  2:242814/4‑150 (MQ=255)
                                                                                                                                 aatggCTGGCCTGAGATATCGCCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGa                 >  2:756524/4‑150 (MQ=255)
                                                                                                                                  cAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAAATCGTCGCTGTTACCTCTGGAAAGGAt                >  2:668967/1‑150 (MQ=255)
                                                                                                                                   aGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATc               <  2:668348/150‑1 (MQ=255)
                                                                                                                                   aGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATc               <  1:1546687/150‑1 (MQ=255)
                                                                                                                                    ggCTGGCCTGAGATATCGCCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCg              >  2:145453/1‑150 (MQ=255)
                                                                                                                                      ctggcctggcGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGcc            <  1:937437/150‑1 (MQ=255)
                                                                                                                                      ctggcctggcGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGcc            >  1:1414415/1‑150 (MQ=255)
                                                                                                                                      ctggcctgagATATCGCCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCGTCGGCGTAATCTTAGGTGCCTTACCGCTCCATTGTCGATACAGGCGTTCCTTATCGGCGCTGTTTGCTCTGGAAAGGATAGcc            >  2:297071/1‑150 (MQ=255)
                                                                                                                                       tggcctggcGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCt           >  2:442071/1‑150 (MQ=255)
                                                                                                                                         gcctggcGATATCTCAGGACAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTcg         <  2:1178093/150‑1 (MQ=255)
                                                                                                                                          cctggcGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTcgc        >  1:998738/1‑150 (MQ=255)
                                                                                                                                             ggcGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGaa     >  1:1504184/1‑150 (MQ=255)
                                                                                                                                               cGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGaaaa   >  1:1380622/1‑150 (MQ=255)
                                                                                                                                                gATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAAc  >  2:21567/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
GTCTGGTTTGCGTGGCATATTAAAGGCCAGAACTACACTGCGGTTATGCATTTTGCTGGCGACTGCGAAACGGTATTAGCTTTAGCGGTGAGGTTTGCCCTCGCCCAGCTGCTGCAATTACTGCTATAACCAGGCTGGCCTGGCGATATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAAC  >  NC_000913/1625163‑1625456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: