Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,016:1 +C 5.8% intergenic (+52/+32) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0161.C 5.8% 430.0 / 19.3 104intergenic (+52/+32)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base . (40/58);  new base C (2/4);  total (42/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

Only 100 of 105 total aligned reads displayed.

TCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCA  >  NC_000913/2389868‑2390164
                                                                                                                                                     |                                                                                                                                                     
tCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGt                                                                                                                                                      >  2:150328/1‑150 (MQ=255)
   ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCGTTCTTTTTCAGAAAAACCGCCGAGCATGTcgg                                                                                                                                                   >  2:42748/1‑150 (MQ=255)
    gAACGCTATGATTTTAATATCGATGAAAGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGTCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcggc                                                                                                                                                  <  2:343483/150‑1 (MQ=255)
       cGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGACATGCTcggcggt                                                                                                                                               <  1:195891/150‑2 (MQ=255)
         cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                            >  2:603209/1‑150 (MQ=255)
           aTGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTc                                                                                                                                          <  2:947152/150‑1 (MQ=255)
           aTGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTc                                                                                                                                          <  1:633649/150‑1 (MQ=255)
           aTGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTc                                                                                                                                          <  1:1596407/150‑1 (MQ=255)
            tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                         <  2:1270895/150‑1 (MQ=255)
            tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                         <  1:1032866/150‑1 (MQ=255)
            tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGGTATGTTTAGAGTATTTTGGCGATCGGCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGACATGCTCGGCGGtttttc                                                                                                                                         <  1:1490322/150‑3 (MQ=255)
             taTTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTg                                                                                                                                        <  1:170636/149‑1 (MQ=255)
             gATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTg                                                                                                                                        <  1:860755/150‑1 (MQ=255)
                tttAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAGCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAGCCGCCGACATGCTCGGCGGtttttctgaaa                                                                                                                                    <  2:1556058/150‑8 (MQ=38)
                tttAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACt                                                                                                                                     <  2:861339/150‑1 (MQ=255)
                tttAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACt                                                                                                                                     <  2:656794/150‑1 (MQ=255)
                tttAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAGCCGCCGACATGCTCGGCGGtttttctgaaa                                                                                                                                    <  2:1588824/150‑8 (MQ=38)
                 ttAATATCGATGAATGCCGGGCAAAATTCAGGCTGGTATGTTTAGAGTCTTTTGGCGCTAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACtt                                                                                                                                    <  2:825315/150‑1 (MQ=255)
                  tAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTa                                                                                                                                   <  1:14152/150‑1 (MQ=255)
                     tatCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                                >  2:342064/1‑150 (MQ=255)
                     aatCGATGAATGGCGGGCATAATTCAGTCAGTTATGTTTAGAGTAGTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                                <  1:870696/149‑1 (MQ=255)
                       tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAg                                                                                                                              >  2:1428213/1‑150 (MQ=255)
                       tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAg                                                                                                                              >  2:1729660/1‑150 (MQ=255)
                        ggATGAATCGCGGGCAAACTTAAGGCAGTTCTGGGTAGAGTATTTTGGCGAAAGTCGTACAGAGTTCACTTAACGCTCTGCGTCTATCAACAATCCCTTCTTTTTCAGAAAACCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                             <  2:276956/149‑1 (MQ=255)
                        cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGCATTACAAAGt                                                                                                                             >  2:1688674/1‑150 (MQ=255)
                        cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                             <  2:1430796/150‑1 (MQ=255)
                        cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                             <  1:483153/150‑1 (MQ=255)
                         gATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTa                                                                                                                            >  1:1681210/1‑150 (MQ=255)
                         gATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTa                                                                                                                            >  1:455320/1‑150 (MQ=255)
                          aTGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCATCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                           <  1:489671/150‑1 (MQ=255)
                             aaTGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAg                                                                                                                        >  2:390855/1‑150 (MQ=255)
                                    ggCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAt                                                                                                                 <  1:1043503/150‑1 (MQ=255)
                                     gCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                                >  1:1049740/1‑150 (MQ=255)
                                      cAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCa                                                                                                               >  2:200110/1‑150 (MQ=255)
                                       aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAg                                                                                                              <  2:1681210/150‑1 (MQ=255)
                                       aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAg                                                                                                              <  2:455320/150‑1 (MQ=255)
                                        aaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGc                                                                                                             >  1:1433361/1‑150 (MQ=255)
                                        aaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATATACTATACCTTCTTTTTCAGAAAAACCGCAGAGCATGTAGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGc                                                                                                             >  1:191136/1‑150 (MQ=255)
                                          aTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                           <  1:703181/150‑1 (MQ=255)
                                           ttCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGc                                                                                                          <  1:82553/150‑1 (MQ=255)
                                             cAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCAt                                                                                                        >  1:1123111/1‑150 (MQ=255)
                                              aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCAtt                                                                                                       >  2:1683465/1‑150 (MQ=255)
                                               gggaGTTATGTTTAGAGTATTATGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCTGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTg                                                                                                      <  2:623217/147‑1 (MQ=255)
                                                ggaGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGc                                                                                                     <  1:923195/148‑1 (MQ=255)
                                                gCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGc                                                                                                     <  2:1356110/150‑1 (MQ=255)
                                                 cAGTTTTGTTTAGAGTATTTTGGAGATAGTCGTTCACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACTTCAGCGGCATTGcg                                                                                                    <  2:1613149/150‑2 (MQ=255)
                                                 cAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGcc                                                                                                    <  1:1687897/150‑1 (MQ=255)
                                                   gTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTATGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCGGACTTAAAAAGTAACAGATTACATCATCGGAATTGCCaa                                                                                                  >  2:733219/1‑150 (MQ=255)
                                                     tATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATAAGCGGCATTGCCAAAc                                                                                                >  2:1085126/1‑150 (MQ=255)
                                                       tGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                              <  1:1289796/150‑1 (MQ=255)
                                                       tGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                              <  1:825378/150‑1 (MQ=255)
                                                       tGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                              >  2:1453670/1‑150 (MQ=255)
                                                       tGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                              <  1:1550091/150‑1 (MQ=255)
                                                         tttAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCACTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCGTTCTGACTTACAAAGTAACAGATTACATCCGCGGCATTGCCAAACGcac                                                                                            >  1:252162/1‑150 (MQ=255)
                                                            agagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAAt                                                                                         >  1:1539881/1‑150 (MQ=255)
                                                             gagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACt                                                                                                                                     >  1:1673144/1‑105 (MQ=255)
                                                             gagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACt                                                                                                                                     <  2:1673144/105‑1 (MQ=255)
                                                              agTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGc                                                                                       <  1:1325073/150‑1 (MQ=255)
                                                                tATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTa                                                                                     <  2:217948/150‑1 (MQ=255)
                                                                     tGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAg                                                                                <  2:1339247/150‑1 (MQ=255)
                                                                      ggCGATAGTCGTACTCAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                               <  2:942888/150‑1 (MQ=255)
                                                                        cGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCgg                                                                             >  1:302831/1‑150 (MQ=255)
                                                                             gTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                        >  2:1163532/1‑150 (MQ=255)
                                                                              tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                       <  1:390855/150‑1 (MQ=255)
                                                                               cGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGc                                                                      <  1:965365/150‑1 (MQ=255)
                                                                                   cacaGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATa                                                                  <  1:384166/150‑1 (MQ=255)
                                                                                     caGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATaca                                                                >  1:76554/1‑150 (MQ=255)
                                                                                      tgTTCACTTAACGCTCTGCGTCTATCTACTATACCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTTAGAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTTGCCTTactc                                                               <  2:487392/149‑3 (MQ=255)
                                                                                         tCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCa                                                            <  1:42748/150‑1 (MQ=255)
                                                                                          cACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAg                                                           <  2:103497/150‑1 (MQ=255)
                                                                                           aCTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGc                                                          >  2:1436973/1‑150 (MQ=255)
                                                                                              tAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                       <  1:1353310/150‑1 (MQ=255)
                                                                                              tAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                       <  2:577190/150‑1 (MQ=255)
                                                                                                   ctctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTAc                                                  <  2:1020609/150‑1 (MQ=255)
                                                                                                    tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTAcc                                                 <  1:84790/150‑1 (MQ=255)
                                                                                                    tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTAcc                                                 <  1:401954/150‑1 (MQ=255)
                                                                                                       gCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAAc                                              <  1:1428213/150‑1 (MQ=255)
                                                                                                       gCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAAc                                              <  1:1729660/150‑1 (MQ=255)
                                                                                                        cGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACa                                             >  1:1604310/1‑150 (MQ=255)
                                                                                                               ctactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCaga                                      >  2:581357/1‑150 (MQ=255)
                                                                                                                tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATTTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCagag                                     >  2:824985/1‑150 (MQ=255)
                                                                                                                tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCCAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTCCCAAAAGTGCagag                                     >  2:80166/1‑150 (MQ=255)
                                                                                                                      cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                               >  1:1043248/1‑150 (MQ=255)
                                                                                                                              tttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGAt                       <  1:1453670/150‑1 (MQ=255)
                                                                                                                               agtcagaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGAt                       >  1:1699044/7‑150 (MQ=38)
                                                                                                                                tCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAc                     <  2:1433361/150‑1 (MQ=255)
                                                                                                                                  aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACGCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                   >  1:1200470/1‑150 (MQ=255)
                                                                                                                                  aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACACCGATAccg                   >  1:407486/1‑150 (MQ=255)
                                                                                                                                  aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                   <  2:689584/150‑1 (MQ=255)
                                                                                                                                  aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                   >  1:127375/1‑150 (MQ=255)
                                                                                                                                   gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgc                  <  1:200110/150‑1 (MQ=255)
                                                                                                                                   agaaagCCGCCGACATGCTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgc                  >  2:1154672/3‑150 (MQ=255)
                                                                                                                                      aaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCCGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccg               >  1:1526074/1‑150 (MQ=255)
                                                                                                                                         ccgccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCgcgc            <  2:76554/150‑1 (MQ=255)
                                                                                                                                             cgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACCATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa        >  1:1271646/1‑150 (MQ=255)
                                                                                                                                                gCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTg     <  2:1024404/150‑1 (MQ=255)
                                                                                                                                                 cATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGc    <  1:419212/150‑1 (MQ=255)
                                                                                                                                                  aTGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGcc   <  1:1422294/150‑1 (MQ=255)
                                                                                                                                                   tGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACCGTGCAGAGATCAGCACACCGATACCGCCCGCGCTGTACTGCCa  >  1:545855/1‑150 (MQ=255)
                                                                                                                                                   tGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCa  >  1:552795/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCCA  >  NC_000913/2389868‑2390164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: