Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,441,661 C→A 5.9% intergenic (‑56/‑103) fabB ← / → mnmC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,441,6610CA 5.9% 310.8 / 4.7 104intergenic (‑56/‑103)fabB/mnmC3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base C (49/48);  new base A (2/5);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TGAACGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCT  >  NC_000913/2441513‑2441808
                                                                                                                                                    |                                                                                                                                                   
tGAACGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGAACAATAGCCTACACt                                                                                                                                                    >  2:1564381/1‑150 (MQ=255)
    cGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTa                                                                                                                                                >  1:1291210/1‑150 (MQ=255)
      tCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACg                                                                                                                                              <  1:1439858/150‑1 (MQ=255)
        cTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGct                                                                                                                                            >  1:261035/1‑149 (MQ=255)
          tCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGa                                                                                                                                          >  1:731467/1‑150 (MQ=255)
          tCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctat                                                                                                                                          <  2:1216793/150‑4 (MQ=255)
          tCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctat                                                                                                                                          <  1:1601610/150‑4 (MQ=255)
            aCGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAAc                                                                                                                                        <  1:295194/150‑1 (MQ=255)
            aCGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctattg                                                                                                                                        <  2:261035/150‑6 (MQ=255)
              gCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACAAGTGTACGctattgtg                                                                                                                                      <  2:824509/150‑8 (MQ=255)
               cAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAg                                                                                                                                     <  2:585007/150‑1 (MQ=255)
                 gagaTGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTc                                                                                                                                   <  1:1144991/150‑1 (MQ=255)
                   gaTGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCg                                                                                                                                 <  1:937393/150‑1 (MQ=255)
                     tGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGAt                                                                                                                               <  1:565523/150‑1 (MQ=255)
                      gCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTTCGGCGTACAAGtgtacgc                                                                                                                              <  1:1731976/150‑7 (MQ=255)
                      gCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATc                                                                                                                              >  2:1655943/1‑150 (MQ=255)
                      gCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATc                                                                                                                              <  2:1241011/150‑1 (MQ=255)
                      gCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAAAGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATc                                                                                                                              <  2:10842/150‑1 (MQ=255)
                       ccAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCAAAATAGCGTACACTTGTACGCCGAACAAGTCCGATCa                                                                                                                             >  1:1295978/1‑150 (MQ=255)
                         aGGACTTCCTGCTGGTTATTACCGATGCTGGTAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGc                                                                                                                           <  2:1371118/150‑1 (MQ=255)
                            aCTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCAt                                                                                                                        >  2:1289659/1‑150 (MQ=255)
                            aCTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCAGTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGACCAAGGCCGATCAGCCAt                                                                                                                        >  2:1066807/1‑150 (MQ=255)
                              ttCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCAttt                                                                                                                      <  2:559352/150‑1 (MQ=255)
                               tcgtgctgGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTa                                                                                                                     <  1:970389/147‑1 (MQ=255)
                                  tgctgGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATa                                                                                                                  >  2:246195/1‑150 (MQ=255)
                                  tgctgGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATa                                                                                                                  >  1:702483/1‑150 (MQ=255)
                                  tgctgGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATa                                                                                                                  >  1:1110565/1‑150 (MQ=255)
                                   gctgGTTATTACCGATGCTGGAAACAATCCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATag                                                                                                                 <  2:295149/150‑1 (MQ=255)
                                   gctgGTTATTACCGATGATGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATag                                                                                                                 >  1:103978/1‑150 (MQ=255)
                                              ccGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTgcg                                                                                                      <  2:1483139/150‑1 (MQ=255)
                                               cGATGCTGGAAACAATGCACAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTCCGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTgcgc                                                                                                     >  2:70168/1‑150 (MQ=255)
                                                    cTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCt                                                                                                >  2:1528315/1‑150 (MQ=255)
                                                    cTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCt                                                                                                <  1:1640058/150‑1 (MQ=255)
                                                    cTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCt                                                                                                >  2:429307/1‑150 (MQ=255)
                                                      ggAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTa                                                                                              <  2:1213712/150‑1 (MQ=255)
                                                      ggAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTa                                                                                              >  2:1145397/1‑150 (MQ=255)
                                                       taaaCAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTac                                                                                             <  1:1297832/149‑1 (MQ=255)
                                                        aaaCAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACATCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTaca                                                                                            >  2:17261/1‑150 (MQ=255)
                                                         aaCAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTacac                                                                                           <  2:578315/150‑1 (MQ=255)
                                                         aaCAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTacac                                                                                           <  1:1164058/150‑1 (MQ=255)
                                                            aaTGCCCAGGCCAGTAATCACTGCACGTTTCATTCAAAACCTCTGTAAGTCGCACATAGAGTTAGTTTCGAAAGCACAAAAGCGTACACTTGTACGCCGAACAAGTCCGATCAGACATTTAATAGAGAAATTTGCTCAGCCTTAcacaca                                                                                        >  2:518018/1‑150 (MQ=255)
                                                                cccAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGATAAATTTGCGCAGCCTTACACACATCGc                                                                                    <  1:1086709/150‑1 (MQ=255)
                                                                  cAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACCCATCGCTa                                                                                  >  2:322496/1‑150 (MQ=255)
                                                                    ggCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAg                                                                                <  2:1525421/150‑1 (MQ=255)
                                                                      ccAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGAt                                                                              <  2:416441/150‑1 (MQ=255)
                                                                      ccAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGAt                                                                              <  1:4804/150‑1 (MQ=255)
                                                                      ccAGTAATCACTGCACGATTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAAAGCACAAAAGCGTACACTTATACGCCGAACAAGACCGATCAACCAATTAATAGAGAAATTATCGCAGCATTACAAACATCGCAAAGaa                                                                              >  1:1628895/1‑149 (MQ=255)
                                                                        aGTAATCACTGCACGTTTCATTAAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTATAACGCCGAACAAGTCCGATCAGCCATTAAATAGAGAAATTTGCTCAGCCTTACACACTTCGCTAAGATCg                                                                            >  1:254603/1‑150 (MQ=255)
                                                                         gTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGa                                                                           <  2:997031/150‑1 (MQ=255)
                                                                          tAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAg                                                                          <  1:1330998/150‑1 (MQ=255)
                                                                             tCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCa                                                                       <  1:1609150/150‑1 (MQ=255)
                                                                                  gCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGTTCGAGCCACCGcc                                                                  <  2:1291210/150‑1 (MQ=255)
                                                                                  gCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGcc                                                                  <  1:544879/150‑1 (MQ=255)
                                                                                      gTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTa                                                              >  2:1469076/1‑150 (MQ=255)
                                                                                        ttcattcaATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTCCACACATCGCTAACATCGAGCCACCGCCTGTAAg                                                            <  2:1398098/150‑1 (MQ=255)
                                                                                          cattcaATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGAc                                                          >  2:17040/1‑150 (MQ=255)
                                                                                           attcaATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACg                                                         >  2:1267696/1‑150 (MQ=255)
                                                                                           attcaATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACg                                                         <  1:246195/150‑1 (MQ=255)
                                                                                           attcaATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACg                                                         <  2:731467/150‑1 (MQ=255)
                                                                                            ttcaATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGa                                                        >  1:308231/1‑150 (MQ=255)
                                                                                              caATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGCCGAGt                                                      >  2:1069374/1‑150 (MQ=255)
                                                                                              caATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGt                                                      <  2:1610918/150‑1 (MQ=255)
                                                                                              caATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGt                                                      >  2:826063/1‑150 (MQ=255)
                                                                                                 tACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAAc                                                   <  1:457267/150‑1 (MQ=255)
                                                                                                    ctctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTa                                                <  1:12441/150‑1 (MQ=255)
                                                                                                    ctctGTAAGTCGCACAAAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTa                                                <  1:1114545/150‑1 (MQ=255)
                                                                                                        gTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTg                                            <  2:1032082/150‑1 (MQ=255)
                                                                                                        gTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTg                                            <  2:51488/150‑1 (MQ=255)
                                                                                                        gTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGACTGTAAGACGAGTAACTTACGTg                                            >  2:1301640/1‑150 (MQ=255)
                                                                                                         tAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGa                                           >  1:84059/1‑150 (MQ=255)
                                                                                                            gacGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCTATCAGCAATTTAATAGAGAAATTTGCGCATCCTTACACAAATAGCTAAGATCGCGCATCAGCCTGTAAGAGGAGTAACTTACGTGAAac                                        >  2:85860/3‑150 (MQ=255)
                                                                                                            gTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAac                                        >  1:967504/1‑150 (MQ=255)
                                                                                                              cGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAacac                                      <  2:990450/150‑1 (MQ=255)
                                                                                                              cGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAacac                                      >  1:384605/1‑150 (MQ=255)
                                                                                                                 acaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACactac                                   >  1:846750/1‑150 (MQ=255)
                                                                                                                    tAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTcc                                >  1:167843/1‑150 (MQ=255)
                                                                                                                     agagTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCa                               >  2:419332/1‑150 (MQ=255)
                                                                                                                      gagTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCAt                              >  2:1468820/1‑150 (MQ=255)
                                                                                                                       agTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATa                             >  2:623961/1‑150 (MQ=255)
                                                                                                                       agTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATa                             >  2:1349892/1‑150 (MQ=255)
                                                                                                                           aGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACAGCCTGTAAGACGAGTAACTTACGTGAAAAACTAATCCATACaca                         >  1:335418/1‑148 (MQ=255)
                                                                                                                                cGAATGCACAATAGCGTACACTTGTACGCCGTACAAGTCCGCTAAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGcc                    <  1:315594/150‑1 (MQ=255)
                                                                                                                                cGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGcc                    >  1:1512372/1‑150 (MQ=255)
                                                                                                                                cGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGcc                    >  1:488852/1‑150 (MQ=255)
                                                                                                                                   aTGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAAc                 >  1:384019/1‑150 (MQ=255)
                                                                                                                                    tGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAAcc                <  2:1263911/150‑1 (MQ=255)
                                                                                                                                    tGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAAcc                >  1:403084/1‑150 (MQ=255)
                                                                                                                                          aTAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCAGTTTATAGAGAAATTTGAGCAGCCGGACACACATCGCTAAGCTCGCGCCCCAGCCTGTAAGACGAGTAAATTACCTCAAACACTACTCCAAACAACCACCCAACCTCGAAt          >  1:104104/1‑150 (MQ=255)
                                                                                                                                           tAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAtt         <  2:1110565/150‑1 (MQ=255)
                                                                                                                                           cggCGTACAAGTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTCCTCCATACAACCTGCCACCCTCGAAtt         >  2:417435/3‑150 (MQ=255)
                                                                                                                                            aGCGTACAATTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAttt        <  2:254603/150‑1 (MQ=255)
                                                                                                                                             gCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTa       >  2:359745/1‑150 (MQ=255)
                                                                                                                                             gCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTa       <  2:1724366/150‑1 (MQ=255)
                                                                                                                                              cGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTaa      <  1:660245/150‑1 (MQ=255)
                                                                                                                                               gTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAAt     <  1:78025/150‑1 (MQ=255)
                                                                                                                                               gTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAAt     <  1:738452/150‑1 (MQ=255)
                                                                                                                                               gTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAAt     <  1:611330/150‑1 (MQ=255)
                                                                                                                                               gTACACTTGTACGCCGAACAAGTCCGATAAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAAt     >  2:196695/1‑150 (MQ=255)
                                                                                                                                                tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAGTTAATg    >  2:522563/1‑150 (MQ=255)
                                                                                                                                                  cacTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt  >  2:28690/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGAACGTCCTTCACGCAGAGATGCCAGGACTTCCTGCTGGTTATTACCGATGCTGGAAACAATGCCCAGGCCAGTAATCACTGCACGTTTCATTCAATACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCT  >  NC_000913/2441513‑2441808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: