Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,446,443 A→G 6.3% L344P (CTG→CCG)  aroC ← chorismate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,446,4430AG 6.3% 332.8 / 9.5 113L344P (CTG→CCG) aroCchorismate synthase
Reads supporting (aligned to +/- strand):  ref base A (46/59);  new base G (3/4);  total (49/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 116 total aligned reads displayed.

GCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGT  >  NC_000913/2446294‑2446591
                                                                                                                                                     |                                                                                                                                                    
gCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCCAGCCGAGCCAGCAGCGCAATCGCGGTTTTATTAATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCAATTTTCTCACGATGCCGTAACa                                                                                                                                                      >  1:412734/1‑150 (MQ=255)
gCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACa                                                                                                                                                      <  1:986297/150‑1 (MQ=255)
   caGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGt                                                                                                                                                   <  1:45489/150‑1 (MQ=255)
   caGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGt                                                                                                                                                   >  2:516484/1‑150 (MQ=255)
     ggTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGa                                                                                                                                                 >  1:1671229/1‑150 (MQ=255)
        tGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCATCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATcc                                                                                                                                              <  2:832321/150‑1 (MQ=255)
           gTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCAtt                                                                                                                                           >  2:937608/1‑150 (MQ=255)
           gTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCAta                                                                                                                                           >  2:243652/1‑149 (MQ=255)
            ttATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAAGCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTa                                                                                                                                          <  1:1046951/150‑1 (MQ=255)
             tATTTTTTGCCACGGCGGCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGGAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGTATATGAGTATTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTaa                                                                                                                                         <  1:79059/150‑1 (MQ=255)
                tttttGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGTTCCAGGAGAGCAATCTCGTTTTTATTCATTTTTTACCCGCGGGGAATATCAGTCTTCACCTCGGCATTATGCGCCCGTTGCCGTAACAGGTGATCCATTtaatc                                                                                                                                      <  2:134135/150‑3 (MQ=255)
                 ttttGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACg                                                                                                                                     >  1:538410/1‑150 (MQ=255)
                    tGCCACGGCGGCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATc                                                                                                                                  <  2:802050/150‑1 (MQ=255)
                     gCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGTCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCg                                                                                                                                 <  2:1390395/150‑1 (MQ=255)
                     gCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGAGGAAAATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAAGAGGTGATCCATGAAAACGATCg                                                                                                                                 >  1:180062/1‑150 (MQ=255)
                      ccACGGCGTCGCTGCCAGGCTGGCGCTACAGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAACCGATCGc                                                                                                                                >  2:325232/1‑150 (MQ=255)
                              tCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCg                                                                                                                        <  1:1588951/150‑1 (MQ=255)
                                 cTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGAGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCtt                                                                                                                     >  2:556262/1‑150 (MQ=255)
                                   gCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCt                                                                                                                   >  1:343077/1‑150 (MQ=255)
                                   gCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCt                                                                                                                   >  1:105801/1‑150 (MQ=255)
                                   gCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTAGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCt                                                                                                                   >  1:343074/1‑150 (MQ=255)
                                    ccAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTg                                                                                                                  >  1:996133/1‑150 (MQ=255)
                                     cAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGc                                                                                                                 >  2:1515411/1‑150 (MQ=255)
                                     cAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGc                                                                                                                 <  1:747518/150‑1 (MQ=255)
                                        gCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGAt                                                                                                              <  2:1149075/150‑1 (MQ=255)
                                         cTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATc                                                                                                             <  2:413763/150‑1 (MQ=255)
                                           ggCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGATTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCTCCCGTGGCCGTAACAGGTGATACATTAAAACGATCGCCAGCATCGCTTCTGCGATCgg                                                                                                           >  1:1569114/1‑150 (MQ=255)
                                            gcgcTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGc                                                                                                          <  2:145584/150‑1 (MQ=255)
                                            gcgcTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGAGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGc                                                                                                          >  2:1226806/1‑150 (MQ=255)
                                             cgcTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCa                                                                                                         >  2:1154905/1‑150 (MQ=255)
                                                tACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTg                                                                                                      <  2:1301567/150‑1 (MQ=255)
                                                   tGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTgcgc                                                                                                   <  2:506661/150‑1 (MQ=255)
                                                    ggCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGcgcg                                                                                                  >  1:1126094/1‑150 (MQ=255)
                                                      cAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTAGCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCAGTTGCCGTAACAGGTGATCCATTAAAACGATCTCCAGCATCGCTTCTGCGATCTGCACTGAGCGGa                                                                                                >  2:12727/1‑150 (MQ=255)
                                                        aGCAGAGCCAGCAGCGCAATCGCTGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATc                                                                                              <  2:926338/150‑1 (MQ=255)
                                                         gCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATTGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATcc                                                                                             <  2:1973/150‑1 (MQ=255)
                                                           agagCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGGACTGGCCTGATCCCg                                                                                           >  2:180799/1‑150 (MQ=255)
                                                           agagCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCg                                                                                           >  2:1381284/1‑150 (MQ=255)
                                                           agagCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCg                                                                                           >  2:390102/1‑150 (MQ=255)
                                                            gagCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCgc                                                                                          >  1:655105/1‑149 (MQ=255)
                                                             agCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATccctgt                                                                                         >  2:1271683/1‑147 (MQ=255)
                                                                   cagcGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAggg                                                                                   <  2:1269159/150‑1 (MQ=255)
                                                                     gcgcAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGAt                                                                                 >  2:917075/1‑150 (MQ=255)
                                                                     gcgcAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGAt                                                                                 >  2:765279/1‑150 (MQ=255)
                                                                       gcAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCg                                                                               <  1:1390922/150‑1 (MQ=255)
                                                                       gcAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGAGAACGGCACTGCGCGGAACACTACACAGGGATCg                                                                               >  2:1616221/1‑150 (MQ=255)
                                                                        cAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGt                                                                              <  2:745719/150‑1 (MQ=255)
                                                                        cAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGt                                                                              >  1:1105902/1‑150 (MQ=255)
                                                                         aaTCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTg                                                                             <  2:105801/150‑1 (MQ=255)
                                                                         aaTCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTg                                                                             <  2:343077/150‑1 (MQ=255)
                                                                         aaTCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACGGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTg                                                                             <  2:343074/150‑1 (MQ=255)
                                                                           tagaggTTTTATTCATTTTTTACGAGCGTGGAATATGAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGAc                                                                           <  2:180062/146‑1 (MQ=255)
                                                                           tCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGAc                                                                           <  1:325232/150‑1 (MQ=255)
                                                                           tCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATAAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGAc                                                                           <  1:1102900/150‑1 (MQ=255)
                                                                                 ttttATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCtt                                                                     <  2:1126094/150‑1 (MQ=255)
                                                                                    tATTCATTTTTTACCCGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGCTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTgg                                                                  <  1:1074390/150‑1 (MQ=255)
                                                                                     aTTGATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCATCATCGATTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGt                                                                 <  1:744996/150‑1 (MQ=255)
                                                                                     aTTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGTGATCGTGACGGCCTTTGGt                                                                 <  2:1393452/150‑1 (MQ=255)
                                                                                     aTTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGt                                                                 >  2:283502/1‑150 (MQ=255)
                                                                                     aTTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGt                                                                 <  1:1733299/150‑1 (MQ=255)
                                                                                     aTTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGt                                                                 >  1:1144120/1‑150 (MQ=255)
                                                                                     aTTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCCTTGGt                                                                 <  2:619496/150‑1 (MQ=255)
                                                                                     aTTCAATTTTTACCAGCGTGGATTATCTGTCTTCACAGCTGCTTTTTGCGCCCGTTGCGTTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGAGCGTGACGGCCTTTGGt                                                                 <  2:1092172/150‑1 (MQ=255)
                                                                                        cATTTTTTTCCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGat                                                              <  1:1147054/150‑1 (MQ=255)
                                                                                         aTTTTTTACCAGCGTGGATTATCAGTCTTCACAACGGCATTTTGCGCCCGTTGCCGTAACTGCTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGatc                                                             <  2:1417944/150‑1 (MQ=255)
                                                                                         aTTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGCTCGCCAGCATACCTTCTGCGATCGGAACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGctc                                                             >  2:1047145/1‑150 (MQ=255)
                                                                                         aTTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGatc                                                             <  1:1725703/150‑1 (MQ=255)
                                                                                          ttttttACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGatca                                                            <  2:577696/150‑1 (MQ=255)
                                                                                             tttACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCAtct                                                         <  1:937608/150‑1 (MQ=255)
                                                                                               tACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGCTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCa                                                       <  1:112326/150‑1 (MQ=255)
                                                                                                aCCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCaa                                                      <  2:1528280/150‑1 (MQ=255)
                                                                                                 ccAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAAc                                                     <  1:917075/150‑1 (MQ=255)
                                                                                                 ccAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAAc                                                     >  2:1372267/1‑150 (MQ=255)
                                                                                                   aGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGCTCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCACctt                                                   >  2:1281325/1‑150 (MQ=255)
                                                                                                        ggAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCg                                              >  1:1420272/1‑150 (MQ=255)
                                                                                                          aaTATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGcc                                            >  2:975119/1‑150 (MQ=255)
                                                                                                          aaTATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGcc                                            <  2:303774/150‑1 (MQ=255)
                                                                                                          aaTATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGcc                                            <  1:1381003/150‑1 (MQ=255)
                                                                                                             atCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCaaa                                         >  2:984919/1‑150 (MQ=255)
                                                                                                             atCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCaaa                                         >  1:1699159/1‑150 (MQ=255)
                                                                                                                   cTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGtt                                   <  2:538410/150‑1 (MQ=255)
                                                                                                                   cTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGtt                                   <  2:552973/150‑1 (MQ=255)
                                                                                                                     tCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCAGACACAGGGATCGTGACGGCCTTTTGTGATCATCTCAACTTCTTCGCCAAAGCGGTTaa                                 >  1:675175/1‑150 (MQ=255)
                                                                                                                      cacaTCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAAt                                <  1:350411/150‑1 (MQ=255)
                                                                                                                       acaTCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATg                               <  1:283502/150‑1 (MQ=255)
                                                                                                                        caTCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATgg                              <  2:534359/150‑1 (MQ=255)
                                                                                                                         ctCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGt                             <  2:218774/149‑1 (MQ=255)
                                                                                                                         aTCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGt                             >  2:1702832/1‑150 (MQ=255)
                                                                                                                         aTCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGt                             >  2:1132548/1‑150 (MQ=255)
                                                                                                                          tCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCTACTTCTTCGCCAAAGCGGTTAATGGTa                            <  1:1154905/150‑1 (MQ=255)
                                                                                                                           cGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTGTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTAc                           >  1:1001471/1‑150 (MQ=255)
                                                                                                                                     cgcCCGTTGCCGTAACATGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCAc                 <  2:1455150/150‑1 (MQ=255)
                                                                                                                                        ccGGTGCCGTAACAGGTGATCCATTAAAACGATAGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATGTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGt              <  1:116210/150‑1 (MQ=255)
                                                                                                                                         cGTTGCCGTAACAGGTGATCCATTAAACCGATCGCCTGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTa             <  1:1616221/150‑1 (MQ=255)
                                                                                                                                         cGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTa             >  2:1454352/1‑150 (MQ=255)
                                                                                                                                           ttGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAAt           <  1:1069836/150‑1 (MQ=255)
                                                                                                                                            tGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATg          <  1:135831/150‑1 (MQ=255)
                                                                                                                                               cGTAACAGGTGATCCATTAACACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATTGTACGACCCGGCACGGTAATGCTg       <  1:883160/150‑1 (MQ=255)
                                                                                                                                                gTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTgg      <  2:1420272/150‑1 (MQ=255)
                                                                                                                                                  aaCAGGTGATCCATTAAGACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAg    >  2:974399/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTTCACATCGGCATTTTGCGCCCGTTGCCGTAACAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGT  >  NC_000913/2446294‑2446591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑