Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,615 C→G 9.9% G145G (GGG→GGC) ‡ yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6150CG 9.9% 324.5 / 22.1 110G145G (GGG→GGC) ‡yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (47/51);  new base G (4/7);  total (52/58)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

Only 100 of 115 total aligned reads displayed.

TCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACT  >  NC_000913/2449467‑2449764
                                                                                                                                                     |                                                                                                                                                     
tcatcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGAcc                                                                                                                                                      <  1:996467/150‑1 (MQ=255)
tcatcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGGCGTGACTATCAGGcc                                                                                                                                                      <  2:1243839/150‑1 (MQ=255)
 catcaAATACGTTGAAAGCGTTTTCTCAGCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACTTAATTCTCTTTCCATGAGAGGTCAGGAGGGTGATCGGAGACGGATGGACATATCGTGTCGTGGGTAGCAGGccc                                                                                                                                                     >  2:1598415/1‑150 (MQ=255)
  atcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCATGGTGAGCAGTTTTGCCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTAGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcccc                                                                                                                                                    >  2:255224/1‑150 (MQ=255)
   tcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCt                                                                                                                                                   >  1:540851/1‑150 (MQ=255)
    caAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTg                                                                                                                                                  >  1:1300806/1‑150 (MQ=255)
        tACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATAc                                                                                                                                              <  1:1536635/150‑1 (MQ=255)
        tACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATAc                                                                                                                                              <  1:133829/150‑1 (MQ=255)
           gattaaaGCGTTTTCTCAACCACACCGCCCGTATACTGCATGGGGAGCAGTTTTGACTTACTGACATAATTGTCATTCCATGAAACGTCATAATGTTGAACATAAACGGATGGACAACTCGTGTCGTGACGTATCAGGGGCCTGATagtc                                                                                                                                            <  1:1072645/146‑4 (MQ=255)
           gTTCAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtc                                                                                                                                            <  1:887442/150‑4 (MQ=255)
            ttGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGGGCCTGATAGTCAc                                                                                                                                         <  1:1207757/150‑1 (MQ=255)
             tGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCAc                                                                                                                                         <  1:1376728/150‑1 (MQ=255)
             tGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAACGTGACGTGACTATAAGGCCCCTGATACGTAAc                                                                                                                                         >  2:334563/1‑150 (MQ=255)
             tGAAAGCGTTTTCTCAACCACACCGCCCGGCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCAc                                                                                                                                         <  1:1370043/150‑1 (MQ=255)
              gAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                                                                                        >  1:1170038/1‑150 (MQ=255)
                aaGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGcc                                                                                                                                      >  1:1581997/1‑150 (MQ=255)
                 aGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGccc                                                                                                                                     >  2:1402592/1‑150 (MQ=255)
                  gCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGcccc                                                                                                                                    <  2:793768/150‑1 (MQ=255)
                   cGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGccccc                                                                                                                                   >  1:1095889/1‑150 (MQ=255)
                    gTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCt                                                                                                                                  >  1:124523/1‑150 (MQ=255)
                     ttttCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg                                                                                                                                 <  1:188447/150‑1 (MQ=255)
                          tcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                                                                            >  1:880274/1‑150 (MQ=255)
                            aaCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGc                                                                                                                          <  1:1642446/150‑1 (MQ=255)
                             aCCACACCGCCCGGCGACTGCAGGGTGATCAGTTTTGAATTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                         <  2:683340/150‑1 (MQ=255)
                                  acCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCaga                                                                                                                    >  1:705577/1‑150 (MQ=255)
                                  acCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACATCACGCCCCCTGGAGAGGCTTCaga                                                                                                                    <  1:38697/150‑1 (MQ=255)
                                   ccGCCCGTCGTCTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                   <  2:162046/150‑1 (MQ=255)
                                   ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                   >  1:175012/1‑150 (MQ=255)
                                   ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                   <  2:1624841/150‑1 (MQ=255)
                                    cGCCCGGCGAATTCAGGCTGAGCAGTTTTGACTTAATGACATAATTCGCATTCCATGAAACGTCATAAGGTTGAACCGACACGGATGGACAAATGGGGTCGTGACTATCAGGCCCATGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                                                                  <  2:289366/150‑1 (MQ=255)
                                       ccGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGCACCCTGCAACGTCACGCCCCCGGGAGAGGCTTCAGAGtcct                                                                                                               >  1:461199/1‑148 (MQ=255)
                                         gTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattc                                                                                                             <  1:1648071/150‑1 (MQ=255)
                                           cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                           >  1:1383415/1‑150 (MQ=255)
                                            gACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCata                                                                                                          <  1:448792/150‑1 (MQ=255)
                                              cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATTAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                        <  1:931323/150‑1 (MQ=255)
                                              cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                        >  1:359173/1‑150 (MQ=255)
                                              cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                        <  1:975799/150‑1 (MQ=255)
                                              cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                        <  2:185021/150‑1 (MQ=255)
                                                  aGGGTGAGCAGTTTTGACTTACTGCCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGACCCCTGGAGAGGATTCAGAGTCATTCATAATaacc                                                                                                    >  1:268842/1‑150 (MQ=255)
                                                  aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                                                                    <  2:880274/150‑1 (MQ=255)
                                                    ggTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaa                                                                                                  <  2:1345806/150‑1 (MQ=255)
                                                     gTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaac                                                                                                 <  2:673052/150‑1 (MQ=255)
                                                         gCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATg                                                                                             >  2:465322/1‑150 (MQ=255)
                                                                tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATCACCAACCATGGACAAGa                                                                                      >  2:486396/1‑150 (MQ=255)
                                                                tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGa                                                                                      >  2:1023422/1‑150 (MQ=255)
                                                                  aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCACCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaaa                                                                                    <  1:220887/150‑1 (MQ=255)
                                                                    ttACTGACCTAGTTGTCATGCCATGAACAGTGATCAGGTTGATCCGAACCGGCGGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTGCAGAGTCTTTGATATTAACCTACCATGGACAAGAAAAg                                                                                  <  2:131530/150‑1 (MQ=255)
                                                                    ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                                                                  <  1:1314856/150‑1 (MQ=255)
                                                                      aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                                                                                <  1:1412246/150‑1 (MQ=255)
                                                                         gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                             >  1:460674/1‑150 (MQ=255)
                                                                           cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACTCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                                                                           >  1:569540/1‑150 (MQ=255)
                                                                             tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                                                                         <  2:603266/150‑1 (MQ=255)
                                                                              aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCaa                                                                        >  1:749594/1‑150 (MQ=255)
                                                                                    cATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATAAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCAATCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                                                                  >  1:131213/1‑150 (MQ=255)
                                                                                    cATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGGGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                                                                  <  2:1242356/150‑1 (MQ=255)
                                                                                     aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                                                                 >  1:366820/1‑150 (MQ=255)
                                                                                      ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                                                                <  1:930700/150‑1 (MQ=255)
                                                                                       tCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag                                                               <  1:373393/150‑1 (MQ=255)
                                                                                        ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGTCCACTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCata                                                              >  2:174697/1‑148 (MQ=255)
                                                                                        ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTAATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga                                                              >  1:859563/1‑150 (MQ=255)
                                                                                         cctGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                                                             <  2:1435488/148‑1 (MQ=255)
                                                                                         cctGAAACGTCATAACGTGCCACCTACACGGACGTACAACTCGGGGCGTGACTATCAGGCCCCGGATACGTCACGCCCCCGGTAGAGGCTGCAGAGTCATTCATAATAACCAACCATGGACAAGAAATGTAAGATTCAATCTCTGCagcg                                                             <  2:120733/148‑3 (MQ=255)
                                                                                         cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                                                             <  1:477923/150‑1 (MQ=255)
                                                                                          aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAgaga                                                            >  2:928517/1‑150 (MQ=255)
                                                                                           tGAAACGTCATAAGGTTTAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGGCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                           <  1:1072671/150‑1 (MQ=255)
                                                                                           tGAAACGTCATAAGGATGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCATCACAGTCATTCATAATAACCAACCAAGGACAAGAAAAATAATATACAATCTCTGCAGacac                                                           >  1:351443/1‑150 (MQ=255)
                                                                                             aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACaa                                                         <  1:1550585/150‑1 (MQ=255)
                                                                                                 gTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAc                                                     <  2:546270/150‑1 (MQ=255)
                                                                                                  tCATAAGGTTGAAGAGAATCTGATTGACAAATCGTGTCGTGACTATCAGGCACCTGATACGTCACGCACCCTGTAGAGGCTTAAGAGTCATTCTTAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc                                                    <  1:255224/150‑1 (MQ=255)
                                                                                                  tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc                                                    <  1:1636805/150‑1 (MQ=255)
                                                                                                  tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc                                                    >  2:707630/1‑150 (MQ=255)
                                                                                                    aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTc                                                  <  2:175012/150‑1 (MQ=255)
                                                                                                     aaaGGTTGAACATAAACGGAATGTAAAATCGTGTAGTTTCTAATATGGCCCTGATACCTCACGGCCCCTGGAGATGCTTCACATTGATTTAAAAAAACCAAACATTGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCa                                                 <  2:882532/149‑1 (MQ=255)
                                                                                                          ttGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGt                                            >  1:203768/1‑150 (MQ=255)
                                                                                                           tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGta                                           >  1:417676/1‑150 (MQ=255)
                                                                                                           tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGta                                           >  1:1176550/1‑150 (MQ=255)
                                                                                                            gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCGTGCATAATAACCCACCATGGACAAGAAAAGTAAGATTAAATCTCTGCAGAGACAATTAACTCAAATGtct                                          >  2:320663/1‑148 (MQ=255)
                                                                                                              aCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTataa                                        >  1:172192/1‑150 (MQ=255)
                                                                                                               cAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTataat                                       <  2:898811/150‑1 (MQ=255)
                                                                                                               cAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGAGACGTCACGCCCCCTGGAGAGGCTGCAGAGTCATTAATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAACTCAAATGTataat                                       >  2:1455826/1‑150 (MQ=255)
                                                                                                                   aaCGGATGGACATATCGTGTCGTGACTATCAGGCACCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATCGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAccc                                   <  1:334563/150‑1 (MQ=255)
                                                                                                                     cGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGc                                 <  1:664651/150‑1 (MQ=255)
                                                                                                                          ggACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTc                            <  2:1170038/150‑1 (MQ=255)
                                                                                                                           gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTcc                           >  2:1026294/1‑150 (MQ=255)
                                                                                                                             cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCAc                         >  2:467843/1‑150 (MQ=255)
                                                                                                                                aTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACAAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCa                      <  2:1581997/150‑1 (MQ=255)
                                                                                                                                       cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTa               >  1:1627113/1‑150 (MQ=255)
                                                                                                                                       cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTa               >  1:1373630/1‑150 (MQ=255)
                                                                                                                                       cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTa               >  1:1370314/1‑150 (MQ=255)
                                                                                                                                        gtgacgtATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCCCCCAGACGGTaa              >  2:920667/7‑150 (MQ=255)
                                                                                                                                        gtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTaa              <  2:1095889/150‑1 (MQ=255)
                                                                                                                                          gACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATc            <  2:203768/150‑1 (MQ=255)
                                                                                                                                              tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATCCCCGCAGTTCCACCAAGACGGTAATCCAg         >  1:1142239/1‑150 (MQ=255)
                                                                                                                                              tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCCGACGGTAATCCAg         >  1:1105073/1‑150 (MQ=255)
                                                                                                                                               aTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg        >  2:326403/1‑150 (MQ=255)
                                                                                                                                               aTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg        >  2:248026/1‑150 (MQ=255)
                                                                                                                                                 cAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACATGAAAAGTAAGATTCAATCTCTGCAGAGAGAATAAGCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGAc      <  1:323180/150‑1 (MQ=255)
                                                                                                                                                  aGGCCCCTGATACGTCACTCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACa     <  2:569540/150‑1 (MQ=255)
                                                                                                                                                   ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAAGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAACTCAAATGTATTATAACAGCAGTTACACCCAGACGGTTATGCAGGACaa    >  2:936887/1‑150 (MQ=255)
                                                                                                                                                     ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACGATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACt  >  1:101827/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACT  >  NC_000913/2449467‑2449764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: