Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,614 C→T 7.3% intergenic (‑145/+36) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,6140CT 7.3% 332.7 / 9.4 108intergenic (‑145/+36)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base C (62/38);  new base T (4/4);  total (66/42)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 112 total aligned reads displayed.

CCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGG  >  NC_000913/2460465‑2460763
                                                                                                                                                     |                                                                                                                                                     
cccATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGc                                                                                                                                                       >  1:839142/1‑150 (MQ=255)
 ccttAACACTCTCTTGTAAAAAAATAAATAAAGTGTTTTGACCTTATTTTAGTCTTAACAATTTTTTTAATTTTAGCCATGTTGATAAAAGGTAACTTGGGAGATTTTACAAATAGAGAAAATAAGAAGAGAAAAGAAAACGCCCCTGcc                                                                                                                                                      <  2:1469999/147‑1 (MQ=255)
 ccATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATAATAGTATTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGcc                                                                                                                                                      <  1:45881/150‑1 (MQ=255)
  cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCa                                                                                                                                                     >  2:571661/1‑150 (MQ=255)
  cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                                                                                     >  1:424292/1‑150 (MQ=255)
  cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                                                                                     >  2:154528/1‑150 (MQ=255)
    tAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGc                                                                                                                                                   <  1:947464/150‑1 (MQ=255)
     aaCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATAATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATTACACAGAGAGAAAAGAAGTAGAGAAAAGAAAACGCCCCTGCCAGCa                                                                                                                                                  >  2:1040990/1‑150 (MQ=255)
      acacTCCCCTGTAAACAAATAAATAAAGTGGTCTGACCTGATCATATTCTTAACAATTTTTTTACATTTAGCCACGTGGAGAAACGGGAATGTGGGAGCTATGACACAGAGCGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCaa                                                                                                                                                 <  2:1382460/150‑1 (MQ=255)
         cTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTg                                                                                                                                              >  2:339122/1‑150 (MQ=255)
                ttAAAAAAATAAATAAAGTGGTCTTACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGACAAAAGGCAAAGGGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                       <  2:1513830/149‑1 (MQ=255)
                gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                       <  1:493013/150‑1 (MQ=255)
                 tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGc                                                                                                                                      <  1:18314/150‑1 (MQ=255)
                 tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                                                                                      <  1:1412065/150‑1 (MQ=255)
                  aaaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGCAGAAAAGGGAAAGTGGTAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcg                                                                                                                                     <  2:742928/150‑1 (MQ=255)
                  aaaaaaaTAAATAAAGTGGTCTGACCTGATAATAGTCTTANCAATTTTTTTAAATTTAGAAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGCAGAGAAAAGAAAACGACCATGCCAGCAACTGACAGTGGcg                                                                                                                                     >  2:894472/1‑150 (MQ=255)
                    aaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAACGGCATAAAAGGGAATGTGTGAGCTATGACCCAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgta                                                                                                                                   <  1:870091/150‑1 (MQ=255)
                     aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTACCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACCCCCCTGCCAGCAACTGACAGGGGcgtac                                                                                                                                  >  2:140215/1‑150 (MQ=255)
                       aataaataAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacgt                                                                                                                                >  1:1500451/1‑150 (MQ=255)
                          aaataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcc                                                                                                                             >  2:908116/1‑150 (MQ=255)
                           aataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                                                                            >  2:1581328/1‑150 (MQ=255)
                            ataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCg                                                                                                                           <  2:722857/150‑1 (MQ=255)
                               aaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAGACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACAGCCAGGGGCGTACGTACCCgaga                                                                                                                        >  2:286031/1‑150 (MQ=255)
                                aGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                       >  2:1719324/1‑150 (MQ=255)
                                 gttgTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAACAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAgg                                                                                                                      <  1:132070/147‑1 (MQ=255)
                                    gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGATAGAAAAGAAGAAGAGAAAAGAAAACGCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                  <  1:1385329/150‑1 (MQ=255)
                                    gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAt                                                                                                                   <  2:1168917/150‑1 (MQ=255)
                                         aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                                                                              >  1:975996/1‑150 (MQ=255)
                                         aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAAAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGTCGCTTACGTACCCGAGAGAAATTAAcc                                                                                                              >  1:338257/1‑149 (MQ=255)
                                          ccTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGTGCGTACGTACCCGAGAGGAATTAACGc                                                                                                             >  1:745620/1‑150 (MQ=255)
                                           cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                                            >  1:425694/1‑150 (MQ=255)
                                               tCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                                                                        <  1:76582/150‑1 (MQ=255)
                                                    gTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                                                                   >  2:1067986/1‑150 (MQ=255)
                                                     tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGATCAAATAACGCAGACCCAGCTg                                                                                                  >  1:324676/1‑150 (MQ=255)
                                                     tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                                                  <  2:1338224/150‑1 (MQ=255)
                                                       ttACCCATTTTTTTACATTTAGCCAAGTGGAGTAAAGGGAAAGTGCTAGCTATGACAAAGATACAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGTATTAACGCAGACCCAGCTGGa                                                                                                <  1:1148495/150‑1 (MQ=255)
                                                       ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                <  2:1383127/150‑1 (MQ=255)
                                                       ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                <  2:757620/150‑1 (MQ=255)
                                                        tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                                                                               <  1:339122/150‑1 (MQ=255)
                                                         aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                                                                              <  2:1236660/150‑1 (MQ=255)
                                                         aaCCATTTTTTTACATTTAGACAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGTGCGTACGTACCCGAGAGGAAATAACGCAGGCCCAGCTGGaaa                                                                                              >  1:395636/1‑150 (MQ=255)
                                                            cATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                                                                           >  2:1034004/1‑150 (MQ=255)
                                                                tttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                                                                       <  1:1034004/150‑1 (MQ=255)
                                                                    tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                   <  2:1500046/150‑1 (MQ=255)
                                                                     aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                  <  1:1596429/150‑1 (MQ=255)
                                                                     aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                  >  2:1582003/1‑150 (MQ=255)
                                                                     aCATTTAGCAAAGTGGATAAAATGGAAAGTGGTAGCTATGAAACATAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATAAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                  <  2:503206/150‑1 (MQ=255)
                                                                      cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                                                                 >  1:435789/1‑150 (MQ=255)
                                                                       aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                                                                >  2:1184267/1‑150 (MQ=255)
                                                                        tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                                               <  2:839142/150‑1 (MQ=255)
                                                                           aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                            >  2:1680798/1‑150 (MQ=255)
                                                                           aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                            >  1:578343/1‑150 (MQ=255)
                                                                            gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCgt                                                                           >  1:1317468/1‑149 (MQ=255)
                                                                            gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                                           >  1:1444255/1‑150 (MQ=255)
                                                                             ccAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                                          >  2:1013645/1‑150 (MQ=255)
                                                                              cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                                                                         <  1:945208/150‑1 (MQ=255)
                                                                               aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                        >  1:865515/1‑150 (MQ=255)
                                                                               aaGTGGAGAAAAGGGAAAGTGGCAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                        <  2:111008/150‑1 (MQ=255)
                                                                                  tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                                                                     >  2:747686/1‑150 (MQ=255)
                                                                                   ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaa                                                                    >  2:232686/1‑150 (MQ=255)
                                                                                     agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                  >  2:768869/1‑150 (MQ=255)
                                                                                     agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                  >  1:1269838/1‑150 (MQ=255)
                                                                                     agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACATACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                  >  1:1017825/1‑150 (MQ=255)
                                                                                        aaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAACCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa                                                               <  2:1232584/150‑1 (MQ=255)
                                                                                           gggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt                                                            >  2:397974/1‑150 (MQ=255)
                                                                                              aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                                         <  2:874248/150‑1 (MQ=255)
                                                                                                 gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat                                                      <  1:1683855/150‑1 (MQ=255)
                                                                                                  tGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc                                                     >  1:1389077/1‑150 (MQ=255)
                                                                                                   gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                    >  2:756394/1‑150 (MQ=255)
                                                                                                   gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                    <  1:1607932/150‑1 (MQ=255)
                                                                                                        cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                               >  1:558187/1‑150 (MQ=255)
                                                                                                          aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                                             >  1:1586799/1‑150 (MQ=255)
                                                                                                                 ccccagaAACGAACCACAGACAAGATAACGCCCCTGCCAGCACCTCTCAGGGGAGTACGTACCCGAGAGGACTGAACCCAGTCCCAGCTCGAAACACCCCATATCCCCTTCGCAGGCACCAGTCCCTTCGATATCCAGGCGCACACCGTc                                      <  2:280092/146‑1 (MQ=255)
                                                                                                                  gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCa                                     <  1:978082/150‑1 (MQ=255)
                                                                                                                    gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                                   >  2:692209/1‑150 (MQ=255)
                                                                                                                     agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAc                                  >  2:1156532/1‑150 (MQ=255)
                                                                                                                        aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCTCAGGCAAAAGTGAACTCGATTTCCAGGCGCACACCGTCAGAgtct                               >  2:54588/1‑150 (MQ=255)
                                                                                                                           gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCa                            >  1:1141779/1‑150 (MQ=255)
                                                                                                                             agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGAGGAAGTAACGCAGACCCAGCTGGAAAATCAGGATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCCGGCGCACACCGTCAGAATCTTCAGt                          >  2:1168939/1‑150 (MQ=255)
                                                                                                                             agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                          <  1:645979/150‑1 (MQ=255)
                                                                                                                             agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                          >  2:436588/1‑150 (MQ=255)
                                                                                                                              gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTg                         <  1:130147/150‑1 (MQ=255)
                                                                                                                                 gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                      <  2:578343/150‑1 (MQ=255)
                                                                                                                                  agaaaagaaaACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAg                     >  2:374072/1‑150 (MQ=255)
                                                                                                                                   gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                    >  1:683423/1‑150 (MQ=255)
                                                                                                                                   gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                    >  2:1353821/1‑150 (MQ=255)
                                                                                                                                    aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACACAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCAAGGCGCACACCGTCAGACTCTTCAGAGAAAGTc                   >  2:1014531/1‑150 (MQ=255)
                                                                                                                                     aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTCAGTGAAAGTCg                  >  1:410708/1‑150 (MQ=255)
                                                                                                                                     aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTGCGCAGGCAAAAGTGAAATCGATATCCAGGCGCCCACCGTCAGCCTCTTCAGTGAAAGTCg                  >  1:1033050/1‑150 (MQ=255)
                                                                                                                                      aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                 <  2:1141779/150‑1 (MQ=255)
                                                                                                                                      aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGCACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                 >  2:1365049/1‑150 (MQ=255)
                                                                                                                                       agaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCggg                >  2:1718312/1‑150 (MQ=255)
                                                                                                                                        gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg               >  2:1190645/1‑150 (MQ=255)
                                                                                                                                              gCCCCTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTAAGTGAAAGTCGGGGTGAttt         >  1:25603/1‑150 (MQ=255)
                                                                                                                                              gCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAttt         >  2:1143875/1‑150 (MQ=255)
                                                                                                                                               ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCTGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt        >  1:348659/1‑150 (MQ=255)
                                                                                                                                                cccTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGCCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGGGAttttt       >  2:813998/1‑149 (MQ=255)
                                                                                                                                                 ccTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc      >  1:1467348/1‑150 (MQ=255)
                                                                                                                                                  cTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCa     <  2:1269838/150‑1 (MQ=255)
                                                                                                                                                     ccAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAgg  <  1:747686/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGG  >  NC_000913/2460465‑2460763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: