Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,618 C→T 6.4% intergenic (‑149/+32) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,6180CT 6.4% 347.7 / 8.2 109intergenic (‑149/+32)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base C (62/40);  new base T (3/4);  total (65/44)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 111 total aligned reads displayed.

TAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCA  >  NC_000913/2460469‑2460767
                                                                                                                                                     |                                                                                                                                                     
tAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGc                                                                                                                                                       <  1:947464/150‑1 (MQ=255)
 aaCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATAATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATTACACAGAGAGAAAAGAAGTAGAGAAAAGAAAACGCCCCTGCCAGCa                                                                                                                                                      >  2:1040990/1‑150 (MQ=255)
  acacTCCCCTGTAAACAAATAAATAAAGTGGTCTGACCTGATCATATTCTTAACAATTTTTTTACATTTAGCCACGTGGAGAAACGGGAATGTGGGAGCTATGACACAGAGCGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCaa                                                                                                                                                     <  2:1382460/150‑1 (MQ=255)
     cTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTg                                                                                                                                                  >  2:339122/1‑150 (MQ=255)
            ttAAAAAAATAAATAACGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACAATACGCCAAGTGGAGAAACGGGAAAGTGGGAGCTATGACAAAGAGATAAAAGAACAATAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAgggg                                                                                                                                           <  1:595599/149‑1 (MQ=255)
            ttAAAAAAATAAATAAAGTGGTCTTACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGACAAAAGGCAAAGGGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                           <  2:1513830/149‑1 (MQ=255)
            gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                           <  1:493013/150‑1 (MQ=255)
             tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGc                                                                                                                                          <  1:18314/150‑1 (MQ=255)
             tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                                                                                          <  1:1412065/150‑1 (MQ=255)
              aaaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGCAGAAAAGGGAAAGTGGTAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcg                                                                                                                                         <  2:742928/150‑1 (MQ=255)
              aaaaaaaTAAATAAAGTGGTCTGACCTGATAATAGTCTTANCAATTTTTTTAAATTTAGAAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGCAGAGAAAAGAAAACGACCATGCCAGCAACTGACAGTGGcg                                                                                                                                         >  2:894472/1‑150 (MQ=255)
                aaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAACGGCATAAAAGGGAATGTGTGAGCTATGACCCAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgta                                                                                                                                       <  1:870091/150‑1 (MQ=255)
                 aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcgttt                                                                                                                                      <  2:1704955/150‑3 (MQ=255)
                 aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTACCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACCCCCCTGCCAGCAACTGACAGGGGcgtac                                                                                                                                      >  2:140215/1‑150 (MQ=255)
                   aataaataAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacgt                                                                                                                                    >  1:1500451/1‑150 (MQ=255)
                      aaataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcc                                                                                                                                 >  2:908116/1‑150 (MQ=255)
                       aataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                                                                                >  2:1581328/1‑150 (MQ=255)
                        ataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCg                                                                                                                               <  2:722857/150‑1 (MQ=255)
                           aaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAGACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACAGCCAGGGGCGTACGTACCCgaga                                                                                                                            >  2:286031/1‑150 (MQ=255)
                            cgTGGTCTGACCTCATCCTCGTCCTACCCATTTTTTACCATTTCCCCCCGTGCAGCAACGCGAAAGCGGGACCAATGACACAGAGACAAAACAAGACGAGAAAAGAAACCGCCCCTGCAAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                           <  2:1045842/149‑1 (MQ=255)
                            aGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                           >  2:1719324/1‑150 (MQ=255)
                             gttgTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAACAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAgg                                                                                                                          <  1:132070/147‑1 (MQ=255)
                                gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGATAGAAAAGAAGAAGAGAAAAGAAAACGCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                      <  1:1385329/150‑1 (MQ=255)
                                gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAt                                                                                                                       <  2:1168917/150‑1 (MQ=255)
                                gTCTGACCTGATCATAGTCTTAACCATTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                      >  2:1401928/1‑150 (MQ=255)
                                     aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                                                                                  >  1:975996/1‑150 (MQ=255)
                                     aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAAAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGTCGCTTACGTACCCGAGAGAAATTAAcc                                                                                                                  >  1:338257/1‑149 (MQ=255)
                                       cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                                                >  1:425694/1‑150 (MQ=255)
                                           tCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                                                                            <  1:76582/150‑1 (MQ=255)
                                                gTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                                                                       >  2:1067986/1‑150 (MQ=255)
                                                 tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGATCAAATAACGCAGACCCAGCTg                                                                                                      >  1:324676/1‑150 (MQ=255)
                                                 tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                                                      <  2:1338224/150‑1 (MQ=255)
                                                   ttACCCATTTTTTTACATTTAGCCAAGTGGAGTAAAGGGAAAGTGCTAGCTATGACAAAGATACAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGTATTAACGCAGACCCAGCTGGa                                                                                                    <  1:1148495/150‑1 (MQ=255)
                                                   ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                    <  2:1383127/150‑1 (MQ=255)
                                                   ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                    <  2:757620/150‑1 (MQ=255)
                                                    tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                                                                                   <  1:339122/150‑1 (MQ=255)
                                                     aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                                                                                  <  2:1236660/150‑1 (MQ=255)
                                                        cATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                                                                               >  2:1034004/1‑150 (MQ=255)
                                                            tttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                                                                           <  1:1034004/150‑1 (MQ=255)
                                                                tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                       <  2:1500046/150‑1 (MQ=255)
                                                                tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                       <  2:751152/150‑1 (MQ=255)
                                                                 aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                      <  1:1596429/150‑1 (MQ=255)
                                                                 aCATTTAGCAAAGTGGATAAAATGGAAAGTGGTAGCTATGAAACATAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATAAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                      <  2:503206/150‑1 (MQ=255)
                                                                  cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                                                                     >  1:435789/1‑150 (MQ=255)
                                                                   aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                                                                    >  2:1184267/1‑150 (MQ=255)
                                                                    tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                                                   <  2:839142/150‑1 (MQ=255)
                                                                     ttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCtt                                                                                  <  2:1215694/150‑1 (MQ=255)
                                                                       aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                                >  2:1680798/1‑150 (MQ=255)
                                                                       aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                                >  1:578343/1‑150 (MQ=255)
                                                                        gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCgt                                                                               >  1:1317468/1‑149 (MQ=255)
                                                                        gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                                               >  1:1444255/1‑150 (MQ=255)
                                                                          cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                                                                             <  1:945208/150‑1 (MQ=255)
                                                                           aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                            >  1:865515/1‑150 (MQ=255)
                                                                           aaGTGGAGAAAAGGGAAAGTGGCAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                            <  2:111008/150‑1 (MQ=255)
                                                                               ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTGCGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCCGCTTCGCAGGCaaa                                                                        >  1:935496/1‑150 (MQ=255)
                                                                               ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaa                                                                        >  2:232686/1‑150 (MQ=255)
                                                                                 agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                      >  1:1269838/1‑150 (MQ=255)
                                                                                 agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                      >  2:768869/1‑150 (MQ=255)
                                                                                 agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACATACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                      >  1:1017825/1‑150 (MQ=255)
                                                                                    aaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAACCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa                                                                   <  2:1232584/150‑1 (MQ=255)
                                                                                          aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                                             <  2:874248/150‑1 (MQ=255)
                                                                                             gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat                                                          <  1:1683855/150‑1 (MQ=255)
                                                                                              tGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc                                                         >  1:1389077/1‑150 (MQ=255)
                                                                                               gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                        >  2:756394/1‑150 (MQ=255)
                                                                                               gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATca                                                        >  2:490060/1‑149 (MQ=255)
                                                                                                    cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                                   >  1:558187/1‑150 (MQ=255)
                                                                                                      aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                                                 >  1:1586799/1‑150 (MQ=255)
                                                                                                            caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCCGCAACTGCCAGGGGCGTACGTACCCGAGAGGAATTACCGCAGACCCAGCTGGAAAATCAGCATTACAGCTTCGCACGCAAACGTGAAAACGATAGCCAGGCGCCCCCCgg                                           >  1:356069/1‑149 (MQ=255)
                                                                                                             ccccagaAACGAACCACAGACAAGATAACGCCCCTGCCAGCACCTCTCAGGGGAGTACGTACCCGAGAGGACTGAACCCAGTCCCAGCTCGAAACACCCCATATCCCCTTCGCAGGCACCAGTCCCTTCGATATCCAGGCGCACACCGTc                                          <  2:280092/146‑1 (MQ=255)
                                                                                                              gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCa                                         <  1:978082/150‑1 (MQ=255)
                                                                                                                gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                                       >  2:692209/1‑150 (MQ=255)
                                                                                                                    aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCTCAGGCAAAAGTGAACTCGATTTCCAGGCGCACACCGTCAGAgtct                                   >  2:54588/1‑150 (MQ=255)
                                                                                                                       gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCa                                >  1:1141779/1‑150 (MQ=255)
                                                                                                                         agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGAGGAAGTAACGCAGACCCAGCTGGAAAATCAGGATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCCGGCGCACACCGTCAGAATCTTCAGt                              >  2:1168939/1‑150 (MQ=255)
                                                                                                                         agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                              >  2:436588/1‑150 (MQ=255)
                                                                                                                         agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                              <  1:645979/150‑1 (MQ=255)
                                                                                                                          gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTg                             <  1:130147/150‑1 (MQ=255)
                                                                                                                             gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                          <  2:578343/150‑1 (MQ=255)
                                                                                                                              agaaaagaaaACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAg                         >  2:374072/1‑150 (MQ=255)
                                                                                                                               gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                        >  1:683423/1‑150 (MQ=255)
                                                                                                                                aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACACAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCAAGGCGCACACCGTCAGACTCTTCAGAGAAAGTc                       >  2:1014531/1‑150 (MQ=255)
                                                                                                                                 aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTCAGTGAAAGTCg                      >  1:410708/1‑150 (MQ=255)
                                                                                                                                 aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTGCGCAGGCAAAAGTGAAATCGATATCCAGGCGCCCACCGTCAGCCTCTTCAGTGAAAGTCg                      >  1:1033050/1‑150 (MQ=255)
                                                                                                                                  aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                     <  2:1141779/150‑1 (MQ=255)
                                                                                                                                  aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGCACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                     >  2:1365049/1‑150 (MQ=255)
                                                                                                                                   agaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCggg                    >  2:1718312/1‑150 (MQ=255)
                                                                                                                                    gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg                   >  2:1190645/1‑150 (MQ=255)
                                                                                                                                    gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg                   >  1:1605530/1‑150 (MQ=255)
                                                                                                                                          gCCCCTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTAAGTGAAAGTCGGGGTGAttt             >  1:25603/1‑150 (MQ=255)
                                                                                                                                          gCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAttt             >  2:1143875/1‑150 (MQ=255)
                                                                                                                                           ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCTGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt            >  1:348659/1‑150 (MQ=255)
                                                                                                                                            cccTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGCCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGGGAttttt           >  2:813998/1‑149 (MQ=255)
                                                                                                                                             ccTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc          >  1:1467348/1‑150 (MQ=255)
                                                                                                                                              cTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCa         <  2:1269838/150‑1 (MQ=255)
                                                                                                                                                 ccAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAgg      <  1:747686/150‑1 (MQ=255)
                                                                                                                                                   aGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGtt    >  2:1558815/1‑150 (MQ=255)
                                                                                                                                                   aGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGtt    >  1:165397/1‑150 (MQ=255)
                                                                                                                                                    gCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTc   <  2:424292/150‑1 (MQ=255)
                                                                                                                                                    gCAACTGACAGGGGCGTAAGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTc   <  2:1131076/150‑1 (MQ=255)
                                                                                                                                                     cAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCa  <  1:1067986/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCA  >  NC_000913/2460469‑2460767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: