Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,619 A→T 6.5% intergenic (‑150/+31) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,6190AT 6.5% 305.4 / 9.1 108intergenic (‑150/+31)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base A (60/39);  new base T (3/4);  total (64/44)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 110 total aligned reads displayed.

AACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCA  >  NC_000913/2460470‑2460767
                                                                                                                                                     |                                                                                                                                                    
aaCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATAATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATTACACAGAGAGAAAAGAAGTAGAGAAAAGAAAACGCCCCTGCCAGCa                                                                                                                                                      >  2:1040990/1‑150 (MQ=255)
 acacTCCCCTGTAAACAAATAAATAAAGTGGTCTGACCTGATCATATTCTTAACAATTTTTTTACATTTAGCCACGTGGAGAAACGGGAATGTGGGAGCTATGACACAGAGCGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCaa                                                                                                                                                     <  2:1382460/150‑1 (MQ=255)
           ttAAAAAAATAAATAACGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACAATACGCCAAGTGGAGAAACGGGAAAGTGGGAGCTATGACAAAGAGATAAAAGAACAATAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAgggg                                                                                                                                           <  1:595599/149‑1 (MQ=255)
           gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                           <  1:493013/150‑1 (MQ=255)
            tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGc                                                                                                                                          <  1:18314/150‑1 (MQ=255)
            tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                                                                                          <  1:1412065/150‑1 (MQ=255)
             aaaaaaaTAAATAAAGTGGTCTGACCTGATAATAGTCTTANCAATTTTTTTAAATTTAGAAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGCAGAGAAAAGAAAACGACCATGCCAGCAACTGACAGTGGcg                                                                                                                                         >  2:894472/1‑150 (MQ=255)
               aaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAACGGCATAAAAGGGAATGTGTGAGCTATGACCCAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgta                                                                                                                                       <  1:870091/150‑1 (MQ=255)
                aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcgttt                                                                                                                                      <  2:1704955/150‑3 (MQ=255)
                aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTACCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACCCCCCTGCCAGCAACTGACAGGGGcgtac                                                                                                                                      >  2:140215/1‑150 (MQ=255)
                  aataaataAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacgt                                                                                                                                    >  1:1500451/1‑150 (MQ=255)
                     aaataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcc                                                                                                                                 >  2:908116/1‑150 (MQ=255)
                      aataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                                                                                >  2:1581328/1‑150 (MQ=255)
                       ataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCg                                                                                                                               <  2:722857/150‑1 (MQ=255)
                          aaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAGACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACAGCCAGGGGCGTACGTACCCgaga                                                                                                                            >  2:286031/1‑150 (MQ=255)
                           cgTGGTCTGACCTCATCCTCGTCCTACCCATTTTTTACCATTTCCCCCCGTGCAGCAACGCGAAAGCGGGACCAATGACACAGAGACAAAACAAGACGAGAAAAGAAACCGCCCCTGCAAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                           <  2:1045842/149‑1 (MQ=255)
                           aGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                           >  2:1719324/1‑150 (MQ=255)
                            gttgTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAACAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAgg                                                                                                                          <  1:132070/147‑1 (MQ=255)
                               gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGATAGAAAAGAAGAAGAGAAAAGAAAACG‑CCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                      <  1:1385329/150‑1 (MQ=255)
                               gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAt                                                                                                                       <  2:1168917/150‑1 (MQ=255)
                               gTCTGACCTGATCATAGTCTTAACCA‑TTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                      >  2:1401928/1‑150 (MQ=255)
                                    aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                                                                                  >  1:975996/1‑150 (MQ=255)
                                    aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAAAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGTCGCTTACGTACCCGAGAGAAATTAAcc                                                                                                                  >  1:338257/1‑149 (MQ=255)
                                     ccTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGTGCGTACGTACCCGAGAGGAATTAACGc                                                                                                                 >  1:745620/1‑150 (MQ=255)
                                          tCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                                                                            <  1:76582/150‑1 (MQ=255)
                                                tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGATCAAATAACGCAGACCCAGCTg                                                                                                      >  1:324676/1‑150 (MQ=255)
                                                tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                                                      <  2:1338224/150‑1 (MQ=255)
                                                  ttACCCATTTTTTTACATTTAGCCAAGTGGAGTAAAGGGAAAGTGCTAGCTATGACAAAGATACAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGTATTAACGCAGACCCAGCTGGa                                                                                                    <  1:1148495/150‑1 (MQ=255)
                                                  ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                    <  2:757620/150‑1 (MQ=255)
                                                  ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                    <  2:1383127/150‑1 (MQ=255)
                                                   tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                                                                                   <  1:339122/150‑1 (MQ=255)
                                                    aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                                                                                  <  2:1236660/150‑1 (MQ=255)
                                                    aaCCATTTTTTTACATTTAGACAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGTGCGTACGTACCCGAGAGGAAATAACGCAGGCCCAGCTGGaaa                                                                                                  >  1:395636/1‑150 (MQ=255)
                                                       cATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                                                                               >  2:1034004/1‑150 (MQ=255)
                                                           tttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                                                                           <  1:1034004/150‑1 (MQ=255)
                                                               tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                       <  2:751152/150‑1 (MQ=255)
                                                               tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                       <  2:1500046/150‑1 (MQ=255)
                                                                aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                      <  1:1596429/150‑1 (MQ=255)
                                                                aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                      >  2:1582003/1‑150 (MQ=255)
                                                                aCATTTAGCAAAGTGGATAAAATGGAAAGTGGTAGCTATGAAACATAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATAAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                      <  2:503206/150‑1 (MQ=255)
                                                                 cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                                                                     >  1:435789/1‑150 (MQ=255)
                                                                  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                                                                    >  2:1184267/1‑150 (MQ=255)
                                                                   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                                                   <  2:839142/150‑1 (MQ=255)
                                                                    ttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCtt                                                                                  <  2:1215694/150‑1 (MQ=255)
                                                                      aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                                >  1:578343/1‑150 (MQ=255)
                                                                      aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                                >  2:1680798/1‑150 (MQ=255)
                                                                       gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCgt                                                                               >  1:1317468/1‑149 (MQ=255)
                                                                       gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                                               >  1:1444255/1‑150 (MQ=255)
                                                                        ccAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                                              >  2:1013645/1‑150 (MQ=255)
                                                                         cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                                                                             <  1:945208/150‑1 (MQ=255)
                                                                          aaGTGGAGAAAAGGGAAAGTGGCAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                            <  2:111008/150‑1 (MQ=255)
                                                                             tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                                                                         >  2:747686/1‑150 (MQ=255)
                                                                              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTGCGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCCGCTTCGCAGGCaaa                                                                        >  1:935496/1‑150 (MQ=255)
                                                                              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaa                                                                        >  2:232686/1‑150 (MQ=255)
                                                                                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                      >  2:768869/1‑150 (MQ=255)
                                                                                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                      >  1:1269838/1‑150 (MQ=255)
                                                                                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACATACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                      >  1:1017825/1‑150 (MQ=255)
                                                                                      gggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt                                                                >  2:397974/1‑150 (MQ=255)
                                                                                         aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                                             <  2:874248/150‑1 (MQ=255)
                                                                                            gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat                                                          <  1:1683855/150‑1 (MQ=255)
                                                                                             tGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc                                                         >  1:1389077/1‑150 (MQ=255)
                                                                                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                        <  1:1607932/150‑1 (MQ=255)
                                                                                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                        >  2:756394/1‑150 (MQ=255)
                                                                                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATca                                                        >  2:490060/1‑149 (MQ=255)
                                                                                                   cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                                   >  1:558187/1‑150 (MQ=255)
                                                                                                     aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                                                 >  1:1586799/1‑150 (MQ=255)
                                                                                                           caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCCGCAACTGCCAGGGGCGTACGTACCCGAGAGGAATTACCGCAGACCCAGCTGGAAAATCAGCATTACAGCTTCGCACGCAAACGTGAAAACGATAGCCAGGCGCCCCCCgg                                           >  1:356069/1‑149 (MQ=255)
                                                                                                            ccccagaAACGAACCACAGACAAGATAACGCCCCTGCCAGCACCTCTCAGGGGAGTACGTACCCGAGAGGACTGAACCCAGTCCCAGCTCGAAACACCCCATATCCCCTTCGCAGGCACCAGTCCCTTCGATATCCAGGCGCACACCGTc                                          <  2:280092/146‑1 (MQ=255)
                                                                                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCa                                         <  1:978082/150‑1 (MQ=255)
                                                                                                               gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                                       >  2:692209/1‑150 (MQ=255)
                                                                                                                agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAc                                      >  2:1156532/1‑150 (MQ=255)
                                                                                                                   aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCTCAGGCAAAAGTGAACTCGATTTCCAGGCGCACACCGTCAGAgtct                                   >  2:54588/1‑150 (MQ=255)
                                                                                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCa                                >  1:1141779/1‑150 (MQ=255)
                                                                                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGAGGAAGTAACGCAGACCCAGCTGGAAAATCAGGATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCCGGCGCACACCGTCAGAATCTTCAGt                              >  2:1168939/1‑150 (MQ=255)
                                                                                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                              <  1:645979/150‑1 (MQ=255)
                                                                                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                              >  2:436588/1‑150 (MQ=255)
                                                                                                                         gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTg                             <  1:130147/150‑1 (MQ=255)
                                                                                                                            gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                          <  2:578343/150‑1 (MQ=255)
                                                                                                                             agaaaagaaaACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAg                         >  2:374072/1‑150 (MQ=255)
                                                                                                                              gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                        >  1:683423/1‑150 (MQ=255)
                                                                                                                              gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                        >  2:1353821/1‑150 (MQ=255)
                                                                                                                               aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACACAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCAAGGCGCACACCGTCAGACTCTTCAGAGAAAGTc                       >  2:1014531/1‑150 (MQ=255)
                                                                                                                                aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTCAGTGAAAGTCg                      >  1:410708/1‑150 (MQ=255)
                                                                                                                                aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTGCGCAGGCAAAAGTGAAATCGATATCCAGGCGCCCACCGTCAGCCTCTTCAGTGAAAGTCg                      >  1:1033050/1‑150 (MQ=255)
                                                                                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                     <  2:1141779/150‑1 (MQ=255)
                                                                                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGCACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                     >  2:1365049/1‑150 (MQ=255)
                                                                                                                                  agaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCggg                    >  2:1718312/1‑150 (MQ=255)
                                                                                                                                   gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg                   >  1:1605530/1‑150 (MQ=255)
                                                                                                                                   gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg                   >  2:1190645/1‑150 (MQ=255)
                                                                                                                                         gCCCCTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTAAGTGAAAGTCGGGGTGAttt             >  1:25603/1‑150 (MQ=255)
                                                                                                                                         gCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAttt             >  2:1143875/1‑150 (MQ=255)
                                                                                                                                          ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCTGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt            >  1:348659/1‑150 (MQ=255)
                                                                                                                                           cccTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGCCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGGGAttttt           >  2:813998/1‑149 (MQ=255)
                                                                                                                                            ccTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc          >  1:1467348/1‑150 (MQ=255)
                                                                                                                                             cTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCa         <  2:1269838/150‑1 (MQ=255)
                                                                                                                                                ccAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAgg      <  1:747686/150‑1 (MQ=255)
                                                                                                                                                  aGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGtt    >  2:1558815/1‑150 (MQ=255)
                                                                                                                                                   gCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTc   <  2:424292/150‑1 (MQ=255)
                                                                                                                                                   gCAACTGACAGGGGCGTAAGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTc   <  2:1131076/150‑1 (MQ=255)
                                                                                                                                                    cAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCa  <  1:1067986/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCA  >  NC_000913/2460470‑2460767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑