Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,624 A→G 7.3% intergenic (‑155/+26) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,6240AG 7.3% 301.8 / 12.2 112intergenic (‑155/+26)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base A (59/40);  new base G (4/4);  total (67/45)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01

Only 100 of 113 total aligned reads displayed.

GTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCC  >  NC_000913/2460481‑2460773
                                                                                                                                               |                                                                                                                                                     
ttAAAAAAATAAATAACGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACAATACGCCAAGTGGAGAAACGGGAAAGTGGGAGCTATGACAAAGAGATAAAAGAACAATAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAgggg                                                                                                                                                 <  1:595599/149‑1 (MQ=255)
gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                                 <  1:493013/150‑1 (MQ=255)
 tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGc                                                                                                                                                <  1:18314/150‑1 (MQ=255)
 tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                                                                                                <  1:1412065/150‑1 (MQ=255)
  aaaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGCAGAAAAGGGAAAGTGGTAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcg                                                                                                                                               <  2:742928/150‑1 (MQ=255)
  aaaaaaaTAAATAAAGTGGTCTGACCTGATAATAGTCTTANCAATTTTTTTAAATTTAGAAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGCAGAGAAAAGAAAACGACCATGCCAGCAACTGACAGTGGcg                                                                                                                                               >  2:894472/1‑150 (MQ=255)
    aaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAACGGCATAAAAGGGAATGTGTGAGCTATGACCCAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgta                                                                                                                                             <  1:870091/150‑1 (MQ=255)
     aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcgttt                                                                                                                                            <  2:1704955/150‑3 (MQ=255)
     aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTACCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACCCCCCTGCCAGCAACTGACAGGGGcgtac                                                                                                                                            >  2:140215/1‑150 (MQ=255)
          aaataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcc                                                                                                                                       >  2:908116/1‑150 (MQ=255)
           aataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                                                                                      >  2:1581328/1‑150 (MQ=255)
            ataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCg                                                                                                                                     <  2:722857/150‑1 (MQ=255)
               aaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAGACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACAGCCAGGGGCGTACGTACCCgaga                                                                                                                                  >  2:286031/1‑150 (MQ=255)
                cgTGGTCTGACCTCATCCTCGTCCTACCCATTTTTTACCATTTCCCCCCGTGCAGCAACGCGAAAGCGGGACCAATGACACAGAGACAAAACAAGACGAGAAAAGAAACCGCCCCTGCAAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                                 <  2:1045842/149‑1 (MQ=255)
                aGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                                 >  2:1719324/1‑150 (MQ=255)
                 gttgTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAACAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAgg                                                                                                                                <  1:132070/147‑1 (MQ=255)
                    gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGATAGAAAAGAAGAAGAGAAAAGAAAACGCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                            <  1:1385329/150‑1 (MQ=255)
                    gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAt                                                                                                                             <  2:1168917/150‑1 (MQ=255)
                    gTCTGACCTGATCATAGTCTTAACCATTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                                            >  2:1401928/1‑150 (MQ=255)
                         aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                                                                                        >  1:975996/1‑150 (MQ=255)
                         aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAAAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGTCGCTTACGTACCCGAGAGAAATTAAcc                                                                                                                        >  1:338257/1‑149 (MQ=255)
                               tCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                                                                                  <  1:76582/150‑1 (MQ=255)
                                    gTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                                                                             >  2:1067986/1‑150 (MQ=255)
                                     tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGATCAAATAACGCAGACCCAGCTg                                                                                                            >  1:324676/1‑150 (MQ=255)
                                     tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                                                            <  2:1338224/150‑1 (MQ=255)
                                       ttACCCATTTTTTTACATTTAGCCAAGTGGAGTAAAGGGAAAGTGCTAGCTATGACAAAGATACAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGTATTAACGCAGACCCAGCTGGa                                                                                                          <  1:1148495/150‑1 (MQ=255)
                                       ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                          <  2:1383127/150‑1 (MQ=255)
                                       ttAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                                                          <  2:757620/150‑1 (MQ=255)
                                        tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                                                                                         <  1:339122/150‑1 (MQ=255)
                                         aaCCATTTTTTTACATTTAGACAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGTGCGTACGTACCCGAGAGGAAATAACGCAGGCCCAGCTGGaaa                                                                                                        >  1:395636/1‑150 (MQ=255)
                                            cATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                                                                                     >  2:1034004/1‑150 (MQ=255)
                                                tttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                                                                                 <  1:1034004/150‑1 (MQ=255)
                                                    tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                             <  2:1500046/150‑1 (MQ=255)
                                                    tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                                             <  2:751152/150‑1 (MQ=255)
                                                     aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                            <  1:1596429/150‑1 (MQ=255)
                                                     aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                                            >  2:1582003/1‑150 (MQ=255)
                                                      cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                                                                           >  1:435789/1‑150 (MQ=255)
                                                       aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                                                                          >  2:1184267/1‑150 (MQ=255)
                                                        tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                                                         <  2:839142/150‑1 (MQ=255)
                                                         ttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCtt                                                                                        <  2:1215694/150‑1 (MQ=255)
                                                           aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                                      >  1:578343/1‑150 (MQ=255)
                                                           aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                                      >  2:1680798/1‑150 (MQ=255)
                                                            gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCgt                                                                                     >  1:1317468/1‑149 (MQ=255)
                                                            gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                                                     >  1:1444255/1‑150 (MQ=255)
                                                             ccAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                                                    >  2:1013645/1‑150 (MQ=255)
                                                              cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                                                                                   <  1:945208/150‑1 (MQ=255)
                                                               aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                                  >  1:865515/1‑150 (MQ=255)
                                                               aaGTGGAGAAAAGGGAAAGTGGCAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                                                  <  2:111008/150‑1 (MQ=255)
                                                                  tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                                                                               >  2:747686/1‑150 (MQ=255)
                                                                   ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTGCGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCCGCTTCGCAGGCaaa                                                                              >  1:935496/1‑150 (MQ=255)
                                                                     agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                            >  2:768869/1‑150 (MQ=255)
                                                                     agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                            >  1:1269838/1‑150 (MQ=255)
                                                                     agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACATACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg                                                                            >  1:1017825/1‑150 (MQ=255)
                                                                        aaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAACCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa                                                                         <  2:1232584/150‑1 (MQ=255)
                                                                           gggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt                                                                      >  2:397974/1‑150 (MQ=255)
                                                                              aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                                                   <  2:874248/150‑1 (MQ=255)
                                                                                 gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat                                                                <  1:1683855/150‑1 (MQ=255)
                                                                                  tGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc                                                               >  1:1389077/1‑150 (MQ=255)
                                                                                   gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                              <  1:1607932/150‑1 (MQ=255)
                                                                                   gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATca                                                              >  2:490060/1‑149 (MQ=255)
                                                                                        cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                                         >  1:558187/1‑150 (MQ=255)
                                                                                          aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                                                       >  1:1586799/1‑150 (MQ=255)
                                                                                                caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCCGCAACTGCCAGGGGCGTACGTACCCGAGAGGAATTACCGCAGACCCAGCTGGAAAATCAGCATTACAGCTTCGCACGCAAACGTGAAAACGATAGCCAGGCGCCCCCCgg                                                 >  1:356069/1‑149 (MQ=255)
                                                                                                 ccccagaAACGAACCACAGACAAGATAACGCCCCTGCCAGCACCTCTCAGGGGAGTACGTACCCGAGAGGACTGAACCCAGTCCCAGCTCGAAACACCCCATATCCCCTTCGCAGGCACCAGTCCCTTCGATATCCAGGCGCACACCGTc                                                <  2:280092/146‑1 (MQ=255)
                                                                                                    gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                                             >  2:692209/1‑150 (MQ=255)
                                                                                                     agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAc                                            >  2:1156532/1‑150 (MQ=255)
                                                                                                        aaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCTCAGGCAAAAGTGAACTCGATTTCCAGGCGCACACCGTCAGAgtct                                         >  2:54588/1‑150 (MQ=255)
                                                                                                           gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCa                                      >  1:1141779/1‑150 (MQ=255)
                                                                                                             agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGAGGAAGTAACGCAGACCCAGCTGGAAAATCAGGATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCCGGCGCACACCGTCAGAATCTTCAGt                                    >  2:1168939/1‑150 (MQ=255)
                                                                                                             agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                                    >  2:436588/1‑150 (MQ=255)
                                                                                                             agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                                    <  1:645979/150‑1 (MQ=255)
                                                                                                                 gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa                                <  2:578343/150‑1 (MQ=255)
                                                                                                                  agaaaagaaaACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAg                               >  2:374072/1‑150 (MQ=255)
                                                                                                                   gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                              >  1:683423/1‑150 (MQ=255)
                                                                                                                   gaaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGt                              >  2:1353821/1‑150 (MQ=255)
                                                                                                                    aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACACAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCAAGGCGCACACCGTCAGACTCTTCAGAGAAAGTc                             >  2:1014531/1‑150 (MQ=255)
                                                                                                                     aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTCAGTGAAAGTCg                            >  1:410708/1‑150 (MQ=255)
                                                                                                                     aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTGCGCAGGCAAAAGTGAAATCGATATCCAGGCGCCCACCGTCAGCCTCTTCAGTGAAAGTCg                            >  1:1033050/1‑150 (MQ=255)
                                                                                                                      aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGCACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                           >  2:1365049/1‑150 (MQ=255)
                                                                                                                       agaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCggg                          >  2:1718312/1‑150 (MQ=255)
                                                                                                                        gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg                         >  1:1605530/1‑150 (MQ=255)
                                                                                                                        gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg                         >  2:1190645/1‑150 (MQ=255)
                                                                                                                              gCCCCTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCGTAAGTGAAAGTCGGGGTGAttt                   >  1:25603/1‑150 (MQ=255)
                                                                                                                              gCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAttt                   >  2:1143875/1‑150 (MQ=255)
                                                                                                                               ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCTGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt                  >  1:348659/1‑150 (MQ=255)
                                                                                                                               ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt                  >  1:1380149/1‑150 (MQ=255)
                                                                                                                                cccTGCCAGCCACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGCCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGGGAttttt                 >  2:813998/1‑149 (MQ=255)
                                                                                                                                 ccTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGc                >  1:1467348/1‑150 (MQ=255)
                                                                                                                                  cTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCa               <  2:1269838/150‑1 (MQ=255)
                                                                                                                                     ccAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAgg            <  1:747686/150‑1 (MQ=255)
                                                                                                                                       agttgcTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGtt          >  2:67779/6‑150 (MQ=255)
                                                                                                                                       aGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGtt          >  2:1558815/1‑150 (MQ=255)
                                                                                                                                       aGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGtt          >  1:165397/1‑150 (MQ=255)
                                                                                                                                        gCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTc         <  2:424292/150‑1 (MQ=255)
                                                                                                                                        gCAACTGACAGGGGCGTAAGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTc         <  2:1131076/150‑1 (MQ=255)
                                                                                                                                         cAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCa        <  1:1067986/150‑1 (MQ=255)
                                                                                                                                           aCTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGCCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCATCAGTGAAAGTCGGGGTGATTTTAAAGGATCAGAt     >  2:269937/1‑150 (MQ=255)
                                                                                                                                            cTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGAt     <  1:1156532/150‑1 (MQ=255)
                                                                                                                                             tGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCCAGGTTCAGAtt    >  1:597944/1‑150 (MQ=255)
                                                                                                                                               aCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTcc  <  1:970002/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCC  >  NC_000913/2460481‑2460773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: