Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,483 A→T 5.7% intergenic (+21/‑332) gtrS → / → tfaS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage/pseudogene, CPS‑53 (KpLE1) prophage; tail fiber assembly protein fragment;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4830AT 5.7% 509.3 / 10.2 177intergenic (+21/‑332)gtrS/tfaSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage/pseudogene, CPS‑53 (KpLE1) prophage; tail fiber assembly protein fragment;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (76/90);  new base T (4/7);  total (80/97)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATGCA  >  NC_000913/2470334‑2470632
                                                                                                                                                      |                                                                                                                                                     
   agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                                                                    >  2:395951/1‑150 (MQ=255)
    gagaTTTAAGCAAGCATTTATTTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                                                                    <  2:929551/150‑1 (MQ=255)
    gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAGTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                                                                   >  2:1043089/1‑150 (MQ=255)
    gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                                                                   >  1:782000/1‑150 (MQ=255)
    gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                                                                   >  2:883556/1‑150 (MQ=255)
       aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACGGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                                                                                <  1:375928/150‑1 (MQ=255)
           aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaat                                                                                                                                            <  2:1163990/150‑3 (MQ=255)
           aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcaca                                                                                                                                            <  1:141095/150‑1 (MQ=255)
            atcaagcaTTTATTTGGAATGTATTTTTCGTTCGCCCTTTGCTTATACATAACAGTCGTATTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGAGATGTAAAGAACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                                                                           >  2:129634/3‑150 (MQ=255)
            agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAATAGCGATGTTAATAAATAATTTTAAATTGAGCGCCAATCATGGCGCGCACaa                                                                                                                                           >  1:1583944/1‑150 (MQ=255)
              caatcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                                                                         <  1:629499/150‑1 (MQ=255)
              caagcaTTTATTTTGAATGTATTTTTCGTTCTACCTTTGCTTATAAATAACATTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAGGTAACGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                                                                         <  2:1273742/150‑1 (MQ=255)
              caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                                                                         >  2:1334805/1‑150 (MQ=255)
              caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                                                                         <  1:898794/150‑1 (MQ=255)
              caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAGTGCGCGCCATGATTGGCGCGcaattta                                                                                                                                         <  2:1601159/150‑6 (MQ=255)
               aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                                                                                        >  2:1000570/1‑150 (MQ=255)
                agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaatttaaa                                                                                                                                       <  1:371473/150‑8 (MQ=255)
                agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCAAATCATGGCGCGCACAAGCta                                                                                                                                       >  1:303085/1‑150 (MQ=255)
                  attttatttGTATTTTATTTTTGGTTCGACCTTTGATTATACATAACATTCGTTTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACAATAGCTATTTAAAGCAATAAGTTTAATTTGCGCGCAAATCTTGGCGCGCACAAGCtata                                                                                                                                     <  2:1425430/148‑1 (MQ=255)
                    tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataat                                                                                                                                   <  1:722450/150‑1 (MQ=255)
                      tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAc                                                                                                                                 <  1:1281884/150‑1 (MQ=255)
                       atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                                                                                >  2:356564/1‑150 (MQ=255)
                            tgAATGTATTTTTCGTTCGAACTTTGCTTAAACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcc                                                                                                                            <  1:863854/149‑1 (MQ=255)
                              aaTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTa                                                                                                                          >  2:686472/1‑150 (MQ=255)
                                 gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                                                                       >  2:1111906/1‑150 (MQ=255)
                                 gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCCAAGTAAAGCACTAAGTTTAAATTTCTCGCCAATCATGGCTCGCACAATATATAATAACAACCTAAtt                                                                                                                       >  1:872149/1‑150 (MQ=255)
                                  tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                                                                                      >  2:41211/1‑150 (MQ=255)
                                      tttCGTTCTACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                                                                  <  1:1294356/150‑1 (MQ=255)
                                      tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                                                                  <  1:1002922/150‑1 (MQ=255)
                                      tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                                                                  <  1:1071586/150‑1 (MQ=255)
                                      tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                                                                  <  1:139336/150‑1 (MQ=255)
                                      tttCGTTCGACCTTTGCTTATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAACCACTAAGTTAAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                                                                  <  1:1287620/150‑1 (MQ=255)
                                                ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACATAATTTCTCCTCCTCTTaga                                                                                                        >  2:1133585/1‑150 (MQ=255)
                                                 cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                                                                                       <  2:1710900/150‑1 (MQ=255)
                                                   ttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                                                                                     <  2:309915/150‑1 (MQ=255)
                                                     gCTTATACATAACAGTCGTTTTTTTATTTATAAAATAATTCAAAGAAATCAAGACAATTGCGATGTAAAGCAGTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCGATAATACCAACATAATTTCTCCGCCTCTTAGAGTGACt                                                                                                  >  2:151280/1‑150 (MQ=255)
                                                      cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTAAAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                                                                                  <  2:863286/150‑1 (MQ=255)
                                                        tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCCCGCCAATCAAGGCGCGCACAAGCTAAAATACCAACATAATTTCTACTCCTCTTAGAGTGACtat                                                                                                >  1:1097699/1‑150 (MQ=255)
                                                          taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAATCACTAATTTTAAATTTAGAGCAAATCATGGCTCGCACAGGCTATAAAAAAAAACTAATTTATACTACTCTTAAAGTGACTatat                                                                                              >  1:226199/1‑150 (MQ=255)
                                                            aaTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtct                                                                                            <  1:52382/149‑1 (MQ=255)
                                                               aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                                                                         <  2:578091/150‑1 (MQ=255)
                                                                   ttCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                                                                     <  1:1275843/149‑1 (MQ=255)
                                                                   gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                                                                     <  1:1201649/150‑1 (MQ=255)
                                                                    tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                                                                    <  2:1378738/150‑1 (MQ=255)
                                                                       tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                                                                 <  2:1259820/150‑1 (MQ=255)
                                                                       tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                                                                 <  1:1732212/150‑1 (MQ=255)
                                                                           tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                                                             <  2:236268/150‑1 (MQ=255)
                                                                            ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                                                                            <  1:525996/150‑1 (MQ=255)
                                                                             tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                                                                           <  1:1127823/150‑1 (MQ=255)
                                                                             aaaTTTAAAAAATAATTCAAATAAATAAACACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                                                                           <  1:1523043/149‑1 (MQ=255)
                                                                                tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAGTTTAAATTGCGCGACATTCATGGCGCGCAAAAGCTATTATACCAACATTATTTCTCCTAGTCTTAGAGTGACTATATATCCTGATAGAATTGAGGTAtt                                                                        >  1:1147796/1‑150 (MQ=255)
                                                                                 ttATAAAATAATTCAAAGAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                                                                       <  1:1444661/150‑1 (MQ=255)
                                                                                  tataAAATAATTCAAATAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                                                                      <  1:1331924/150‑1 (MQ=255)
                                                                                  tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                                                                      >  2:978505/1‑150 (MQ=255)
                                                                                    taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACACGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                                                                    <  2:1681111/150‑1 (MQ=255)
                                                                                      aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                                                                  <  2:240306/150‑1 (MQ=255)
                                                                                      aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                                                                  <  1:249365/150‑1 (MQ=255)
                                                                                              cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                                                                     >  2:1676459/1‑139 (MQ=255)
                                                                                              cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc                                                          >  1:1243677/1‑150 (MQ=255)
                                                                                              cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc                                                          >  2:677032/1‑150 (MQ=255)
                                                                                               aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCACCCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATAGCGGTATTGACTATCAAATGcca                                                         >  2:1699200/1‑149 (MQ=255)
                                                                                               aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc                                                         >  1:1544676/1‑150 (MQ=255)
                                                                                               aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc                                                         >  1:1633197/1‑150 (MQ=255)
                                                                                               aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc                                                         >  2:1639299/1‑150 (MQ=255)
                                                                                               aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcca                                                         >  1:1537954/1‑149 (MQ=255)
                                                                                                aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt                                                        >  2:525286/1‑150 (MQ=255)
                                                                                                aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt                                                        <  2:612556/150‑1 (MQ=255)
                                                                                                aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt                                                        >  1:1061129/1‑150 (MQ=255)
                                                                                                aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt                                                        >  2:1539543/1‑150 (MQ=255)
                                                                                                    aaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAtt                                                    <  2:441835/150‑1 (MQ=255)
                                                                                                     aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc                                                   <  1:455366/150‑1 (MQ=255)
                                                                                                       cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtct                                                                                            >  1:401371/1‑107 (MQ=255)
                                                                                                        aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt                                                <  1:1138241/150‑1 (MQ=255)
                                                                                                         aGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttg                                               <  1:1000410/150‑1 (MQ=255)
                                                                                                           aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCACTGATTCgttgtt                                             >  2:726156/1‑150 (MQ=255)
                                                                                                            cAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGTTATTGACTATCAAATGCCCTGATTCGTTGttt                                            >  1:847444/1‑150 (MQ=255)
                                                                                                                aGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTg                                        <  2:623957/150‑1 (MQ=255)
                                                                                                                 tcGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGt                                       <  1:477463/149‑1 (MQ=255)
                                                                                                                    aTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAat                                    <  2:104278/150‑1 (MQ=255)
                                                                                                                    aTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCCCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAat                                    <  2:1468247/150‑1 (MQ=255)
                                                                                                                       tAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTTTATCTCCTGATAGAATTGCGGTATTGACTATCAAAGGCCCTGATTCGTTGTTTATTGTAatatg                                 >  2:823490/1‑149 (MQ=255)
                                                                                                                          aGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATctcc                              <  2:70353/150‑1 (MQ=255)
                                                                                                                          aGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATctcc                              <  1:1026880/150‑1 (MQ=255)
                                                                                                                            cACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATctcctc                            <  2:779811/150‑1 (MQ=255)
                                                                                                                             aCTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCctct                           <  1:717321/150‑1 (MQ=255)
                                                                                                                              cTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATctcctcaa                          >  2:489664/1‑148 (MQ=255)
                                                                                                                                aaGTTTAAATTGCGCTCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTTACTATATCTCCTGTTAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATc                        <  1:130572/150‑1 (MQ=255)
                                                                                                                                  gTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTGATTGTAATATCTCCTCTATCTg                      >  2:1647438/1‑150 (MQ=255)
                                                                                                                                    ttAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCa                    <  2:710560/150‑1 (MQ=255)
                                                                                                                                     gcttgtGCGCGCCATGATTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAg                   >  1:166118/6‑150 (MQ=255)
                                                                                                                                       aaTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGAc                 <  2:1720366/150‑1 (MQ=255)
                                                                                                                                        aTTGCGCGCCAATCATGGCGAGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACg                <  2:872149/150‑1 (MQ=255)
                                                                                                                                         ttGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGGGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGa               >  1:915453/1‑150 (MQ=255)
                                                                                                                                         gtGCGCGCCATGATTGGCGCGCACAATCTATAATACAAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGa               >  1:347698/2‑150 (MQ=255)
                                                                                                                                          tGCGCGCCATGATTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                                                                                     >  2:873161/1‑63 (MQ=37)
                                                                                                                                          tGCGCGCCATGATTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                                                                                     <  1:873161/63‑1 (MQ=37)
                                                                                                                                          tGCGCGCCATGATTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGAt              >  1:1368452/1‑150 (MQ=255)
                                                                                                                                             gcgcCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAAc           <  2:329207/150‑1 (MQ=255)
                                                                                                                                                  aaTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTaaa      <  1:1043089/150‑1 (MQ=255)
                                                                                                                                                    tCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATg    <  1:58869/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATGCA  >  NC_000913/2470334‑2470632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: