Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,477,014 C→A 12.0% A206S (GCA→TCA)  dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,0140CA12.0% 182.7 / 16.8 82A206S (GCA→TCA) dsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (18/50);  new base A (2/8);  total (24/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

TCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAA  >  NC_000913/2476865‑2477163
                                                                                                                                                     |                                                                                                                                                      
tcttcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGc                                                                                                                                                        >  2:1041414/1‑150 (MQ=255)
 cttcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGcc                                                                                                                                                       <  2:1442880/150‑1 (MQ=255)
  ttcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCct                                                                                                                                                      >  1:421862/1‑150 (MQ=255)
   tcCCATCGCCACCCCAATATGTTTCATCGCGGCAATAACAGTTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAAACGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctg                                                                                                                                                     <  1:1088264/150‑1 (MQ=255)
   tcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctg                                                                                                                                                     <  1:1041414/150‑1 (MQ=255)
   tcCCATCGCCACCCCAATATGATTCATCGAGGCAATAACAGCTAAAGCAGAACGATCAATGCCAATTCCAGAAGATGTCGGCAACTTAACCGCATAATGTTGCGCGCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCGATGCctg                                                                                                                                                     <  1:261911/150‑1 (MQ=255)
        tCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgtctgt                                                                                                                                                <  1:375055/150‑1 (MQ=255)
          gCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCa                                                                                                                                              <  1:1447864/150‑1 (MQ=255)
           ccACCCCATTATGATTAATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGTAGATGTCGGCAAATTAACCGCATAATTCTGCGCCCAACTATGCCATTCATCCGAACCGGAGTCGTTGCTCCATGCCTGTCTGTCAt                                                                                                                                             <  2:70203/150‑1 (MQ=255)
           ccACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGCTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCctgtcggtgca                                                                                                                                             >  1:111513/1‑147 (MQ=255)
              ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCTTACCGGAGTCGTTGATCCAGGGCTGGCTGTGAttgg                                                                                                                                          >  2:301556/1‑147 (MQ=255)
              ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGa                                                                                                                                          <  2:846675/150‑1 (MQ=255)
              ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGa                                                                                                                                          <  2:375047/150‑1 (MQ=255)
                  aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                      <  2:479662/150‑1 (MQ=255)
                  aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                      <  1:1634749/150‑1 (MQ=255)
                   atatGATTCATCGCGGCAATAACAGCTAAATCAGATCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                     <  1:1703771/150‑1 (MQ=255)
                   atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCCGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                     <  2:1157004/150‑1 (MQ=255)
                   atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAAGCGCATATTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAc                                                                                                                                     <  1:208638/150‑1 (MQ=255)
                   atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                     <  1:979411/150‑1 (MQ=255)
                   atatGAATCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGACAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCGCCATGACAGACAGGCATGGAGCAAc                                                                                                                                     <  2:127759/150‑1 (MQ=255)
                    tatGATTCATAGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAACg                                                                                                                                    <  2:821138/150‑1 (MQ=255)
                      tGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTa                                                                                                                                  <  1:480599/150‑1 (MQ=255)
                           cATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGTGCAACGTACAGTg                                                                                                                             <  1:873292/150‑1 (MQ=255)
                             tCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCTGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGac                                                                                                                           <  1:1012691/150‑1 (MQ=255)
                                 ggCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAg                                                                                                                      <  2:1553453/150‑1 (MQ=255)
                                   cAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGACTGTCATGGAGCAACGTACAGTGACACAGGt                                                                                                                    <  1:301556/150‑1 (MQ=255)
                                      tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTaa                                                                                                                 <  2:1536714/150‑1 (MQ=255)
                                       aaCAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCACCCAGGCTGTCGGTACTGTATCAACCTTCAATGACACATGTTTAt                                                                                                                >  2:1177468/1‑150 (MQ=255)
                                           gCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCAGTCTGTCAATGAGCAACCTAAAGTGACACAGGGTTAATAAc                                                                                                             >  1:6322/1‑150 (MQ=255)
                                                 tCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTg                                                                                                      <  1:767576/150‑1 (MQ=255)
                                                  cAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTgg                                                                                                     <  2:1363879/150‑1 (MQ=255)
                                                  cAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCAGGCAGTCATGGAGCAACGTACAAGGACACCGGGTAATTACCGGGTTgg                                                                                                     >  1:1175469/1‑150 (MQ=255)
                                                   aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCAACAAGCCTGTCTGTCATGGAGCAACGTTCATTGACACAGGTTTATTACCCTGTTggg                                                                                                    >  2:1132101/1‑149 (MQ=255)
                                                   aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTTCACCAATCCAGTATGTAAAGGTGCAACGGTAAGGTCCACCGGTTAATTAACctgtgtgg                                                                                                    >  2:130901/1‑146 (MQ=255)
                                                    gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGGCTGTCTGTGATGGAGCAACGTACAGTGGCACAGGGTTATTAACGtggtgggt                                                                                                   >  1:1137557/1‑148 (MQ=255)
                                                    gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTAACGTGTTGGtt                                                                                                   >  2:916011/1‑150 (MQ=255)
                                                    gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                   <  1:1506145/150‑1 (MQ=255)
                                                    aaaCGATCAAAGCCAATTCCAGACCATGTCGGCAAATAAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                   <  2:1570206/149‑1 (MQ=255)
                                                      aCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCAATCATCCGTACCGGAGTCGTTGCACCATGGCAGTCTGTCATGGTGCAACGTACAGTGACACAGGTTTAATACCGGGTTGGTTaa                                                                                                 >  1:224075/1‑150 (MQ=255)
                                                        gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCGGTCTGTCATGGTGCAACGTACAGTGTCCCCGGGTTATTACCGTGTTGGGTAAAg                                                                                               >  1:243565/1‑150 (MQ=255)
                                                            aaaGACAATTCCAGAAGTTGTCGGCAAATTAACCGATTAATGTTGCGCCCATCTATGCCATTCATACGTTCGGGTGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACTGTGACACAGGTTAATTACCGTGTTGGTTATAGGATg                                                                                           <  2:104537/150‑1 (MQ=255)
                                                             aaGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGt                                                                                          <  1:664356/150‑1 (MQ=255)
                                                                ccAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAAGTACAGTGACACAGGTTAATTAACGTGTTGGTTTAAGCATGTcat                                                                                       >  2:18632/1‑148 (MQ=255)
                                                                    ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                   <  2:1170195/150‑1 (MQ=255)
                                                                    ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                   <  1:873322/150‑1 (MQ=255)
                                                                      ccAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCg                                                                                 <  2:957323/150‑1 (MQ=255)
                                                                       cAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGt                                                                                <  2:367886/150‑1 (MQ=255)
                                                                              tGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGATCAAAGCCTGTCTGTCATGGAGGAAAGTACAGTGACAAAGGGTTATTAAAGTGTTGGGTAAAGCATGTATTTGAGCGttatcagg                                                                         >  1:1108807/1‑143 (MQ=255)
                                                                                tCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                       <  2:421862/150‑1 (MQ=255)
                                                                                gcGGCAAATTACCCGCATAATGGTGCGCCCAACTATGCCATTCTTCCGTACCGGAGTCGTTGCTGCATGCCTGGCTGTCAAGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                       <  1:366750/149‑1 (MQ=255)
                                                                                gcGGCAAATTAAACGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                       <  2:1076105/149‑1 (MQ=255)
                                                                                   gCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCATATTCCGGGCTAc                                                                    <  2:280551/150‑1 (MQ=255)
                                                                                        ttAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGGCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACt                                                               <  1:1432519/150‑1 (MQ=255)
                                                                                           aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATTCCTGTCTGTCATGGTGCAACGTAAAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGATCCGATTACGGGCTAAATACTGGc                                                            >  2:1242495/1‑150 (MQ=255)
                                                                                           aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGc                                                            <  2:29020/150‑1 (MQ=255)
                                                                                                 tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                      <  2:127227/150‑1 (MQ=255)
                                                                                                   aTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttc                                                    <  1:330515/150‑1 (MQ=255)
                                                                                                      ttGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttcttc                                                 <  2:1401923/150‑1 (MQ=255)
                                                                                                      ttGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttcttc                                                 <  1:1110112/150‑1 (MQ=255)
                                                                                                       tGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTca                                                <  2:392955/150‑1 (MQ=255)
                                                                                                          gcCCAACTCTGCCATTCATCCGTCCCGGAGTCGTGGCTCCATGCCTGTCTGTCATGGAGGAACGTACAGTGACACAGGTTAATTACCTTGTTGGTTAAATCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcc                                             <  1:1223270/150‑1 (MQ=255)
                                                                                                              aaCTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCa                                         <  1:437764/150‑1 (MQ=255)
                                                                                                                 tATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCTGTATGGCAAGGAGCCACGTACAGTGCCCCAAGTAAATTACCGTTTTTGTTTAAGCATGGCTTTGAGCGTATTCAGGGCGACATACTGTCAGGATTTATTaatacataacaa                                      >  2:912835/1‑145 (MQ=255)
                                                                                                                   tGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAg                                    <  1:832487/150‑1 (MQ=255)
                                                                                                                     ccATTCCTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtg                                  <  2:209213/150‑1 (MQ=255)
                                                                                                                          aaTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgag                             <  2:1348507/149‑1 (MQ=255)
                                                                                                                           aTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgagt                            <  2:6322/150‑1 (MQ=255)
                                                                                                                            tCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGTAGGATTTCTTCATCCATCAGAAAGTGAtgagtg                           <  2:232869/150‑1 (MQ=255)
                                                                                                                                     gAGGCGTTGCTCCATCCCTGTCTGTCATGGAGCAACGTACAGTGCCACAGGTTAATTACCGTGTAGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGGTGCGCt                  <  2:339815/150‑1 (MQ=255)
                                                                                                                                      aGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTg                 <  1:1437515/150‑1 (MQ=255)
                                                                                                                                        tCGTTGCTCCATGCCTGACTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGAc               <  2:827819/150‑1 (MQ=255)
                                                                                                                                         cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg              >  1:462468/1‑150 (MQ=255)
                                                                                                                                         cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg              >  2:370198/1‑150 (MQ=255)
                                                                                                                                           ttGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc            <  1:552228/150‑1 (MQ=255)
                                                                                                                                           ttGCTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc            >  2:29976/1‑150 (MQ=255)
                                                                                                                                             gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc          >  1:1193442/1‑150 (MQ=255)
                                                                                                                                             gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc          >  1:1085421/1‑150 (MQ=255)
                                                                                                                                              cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca         <  2:1506956/150‑1 (MQ=255)
                                                                                                                                              cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGAGTTGGTTAAAGAATGACTTTGAGCGTATTCCGGGCTACATACTGGAAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGATGCGCTGACGGCGca         >  1:667304/1‑150 (MQ=255)
                                                                                                                                              cTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca         >  1:1604082/1‑150 (MQ=255)
                                                                                                                                                 cATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatca      <  1:1132101/150‑1 (MQ=255)
                                                                                                                                                    gCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTACCGTGTTGGGTAAAGCCTGTCTTTGAGCGTCTTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGCAAGTGCTGAGTGAGTTGCGCCGTCAGCGcaactcac   <  1:784712/150‑7 (MQ=255)
                                                                                                                                                    gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCAtcatca   >  1:1124170/1‑150 (MQ=255)
                                                                                                                                                    gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGGTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcat   <  1:130901/150‑7 (MQ=255)
                                                                                                                                                     ccTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcact  <  1:1729434/150‑8 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAA  >  NC_000913/2476865‑2477163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: