Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,477,016 T→A 17.4% Q205L (CAG→CTG)  dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,0160TA17.4% 137.2 / 20.8 77Q205L (CAG→CTG) dsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (15/47);  new base A (6/8);  total (22/55)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

TTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAA  >  NC_000913/2476867‑2477164
                                                                                                                                                     |                                                                                                                                                     
ttcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCct                                                                                                                                                       >  1:421862/1‑150 (MQ=255)
 tcCCATCGCCACCCCAATATGTTTCATCGCGGCAATAACAGTTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAAACGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctg                                                                                                                                                      <  1:1088264/150‑1 (MQ=255)
 tcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctg                                                                                                                                                      <  1:1041414/150‑1 (MQ=255)
 tcCCATCGCCACCCCAATATGATTCATCGAGGCAATAACAGCTAAAGCAGAACGATCAATGCCAATTCCAGAAGATGTCGGCAACTTAACCGCATAATGTTGCGCGCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCGATGCctg                                                                                                                                                      <  1:261911/150‑1 (MQ=255)
      tCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgtctgt                                                                                                                                                 <  1:375055/150‑1 (MQ=255)
        gCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCa                                                                                                                                               <  1:1447864/150‑1 (MQ=255)
         ccACCCCATTATGATTAATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGTAGATGTCGGCAAATTAACCGCATAATTCTGCGCCCAACTATGCCATTCATCCGAACCGGAGTCGTTGCTCCATGCCTGTCTGTCAt                                                                                                                                              <  2:70203/150‑1 (MQ=255)
         ccACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGCTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCctgtcggtgca                                                                                                                                              >  1:111513/1‑147 (MQ=255)
            ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCTTACCGGAGTCGTTGATCCAGGGCTGGCTGTGAttgg                                                                                                                                           >  2:301556/1‑147 (MQ=255)
            ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGa                                                                                                                                           <  2:846675/150‑1 (MQ=255)
            ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGa                                                                                                                                           <  2:375047/150‑1 (MQ=255)
                aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                       <  1:1634749/150‑1 (MQ=255)
                aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                       <  2:479662/150‑1 (MQ=255)
                 atatGATTCATCGCGGCAATAACAGCTAAATCAGATCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                      <  1:1703771/150‑1 (MQ=255)
                 atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCCGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                      <  2:1157004/150‑1 (MQ=255)
                 atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAAGCGCATATTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAc                                                                                                                                      <  1:208638/150‑1 (MQ=255)
                 atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                      <  1:979411/150‑1 (MQ=255)
                 atatGAATCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGACAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCGCCATGACAGACAGGCATGGAGCAAc                                                                                                                                      <  2:127759/150‑1 (MQ=255)
                  tatGATTCATAGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAACg                                                                                                                                     <  2:821138/150‑1 (MQ=255)
                    tGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTa                                                                                                                                   <  1:480599/150‑1 (MQ=255)
                         cATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGTGCAACGTACAGTg                                                                                                                              <  1:873292/150‑1 (MQ=255)
                           tCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCTGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGac                                                                                                                            <  1:1012691/150‑1 (MQ=255)
                               ggCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAg                                                                                                                       <  2:1553453/150‑1 (MQ=255)
                                 cAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGACTGTCATGGAGCAACGTACAGTGACACAGGt                                                                                                                     <  1:301556/150‑1 (MQ=255)
                                    tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTaa                                                                                                                  <  2:1536714/150‑1 (MQ=255)
                                     aaCAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCACCCAGGCTGTCGGTACTGTATCAACCTTCAATGACACATGTTTAt                                                                                                                 >  2:1177468/1‑150 (MQ=255)
                                         gCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCAGTCTGTCAATGAGCAACCTAAAGTGACACAGGGTTAATAAc                                                                                                              >  1:6322/1‑150 (MQ=255)
                                               tCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTg                                                                                                       <  1:767576/150‑1 (MQ=255)
                                                cAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTgg                                                                                                      <  2:1363879/150‑1 (MQ=255)
                                                cAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCAGGCAGTCATGGAGCAACGTACAAGGACACCGGGTAATTACCGGGTTgg                                                                                                      >  1:1175469/1‑150 (MQ=255)
                                                 aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCAACAAGCCTGTCTGTCATGGAGCAACGTTCATTGACACAGGTTTATTACCCTGTTggg                                                                                                     >  2:1132101/1‑149 (MQ=255)
                                                 aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTTCACCAATCCAGTATGTAAAGGTGCAACGGTAAGGTCCACCGGTTAATTAACctgtgtgg                                                                                                     >  2:130901/1‑146 (MQ=255)
                                                  gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGGCTGTCTGTGATGGAGCAACGTACAGTGGCACAGGGTTATTAACGtggtgggt                                                                                                    >  1:1137557/1‑148 (MQ=255)
                                                  gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTAACGTGTTGGtt                                                                                                    >  2:916011/1‑150 (MQ=255)
                                                  gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                    <  1:1506145/150‑1 (MQ=255)
                                                  aaaCGATCAAAGCCAATTCCAGACCATGTCGGCAAATAAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                    <  2:1570206/149‑1 (MQ=255)
                                                    aCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCAATCATCCGTACCGGAGTCGTTGCACCATGGCAGTCTGTCATGGTGCAACGTACAGTGACACAGGTTTAATACCGGGTTGGTTaa                                                                                                  >  1:224075/1‑150 (MQ=255)
                                                      gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCGGTCTGTCATGGTGCAACGTACAGTGTCCCCGGGTTATTACCGTGTTGGGTAAAg                                                                                                >  1:243565/1‑150 (MQ=255)
                                                          aaaGACAATTCCAGAAGTTGTCGGCAAATTAACCGATTAATGTTGCGCCCATCTATGCCATTCATACGTTCGGGTGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACTGTGACACAGGTTAATTACCGTGTTGGTTATAGGATg                                                                                            <  2:104537/150‑1 (MQ=255)
                                                           aaGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGt                                                                                           <  1:664356/150‑1 (MQ=255)
                                                              ccAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAAGTACAGTGACACAGGTTAATTAACGTGTTGGTTTAAGCATGTcat                                                                                        >  2:18632/1‑148 (MQ=255)
                                                                  ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                    <  1:873322/150‑1 (MQ=255)
                                                                  ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                    <  2:1170195/150‑1 (MQ=255)
                                                                    ccAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCg                                                                                  <  2:957323/150‑1 (MQ=255)
                                                                     cAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGt                                                                                 <  2:367886/150‑1 (MQ=255)
                                                                            tGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGATCAAAGCCTGTCTGTCATGGAGGAAAGTACAGTGACAAAGGGTTATTAAAGTGTTGGGTAAAGCATGTATTTGAGCGttatcagg                                                                          >  1:1108807/1‑143 (MQ=255)
                                                                              tCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                        <  2:421862/150‑1 (MQ=255)
                                                                              gcGGCAAATTACCCGCATAATGGTGCGCCCAACTATGCCATTCTTCCGTACCGGAGTCGTTGCTGCATGCCTGGCTGTCAAGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                        <  1:366750/149‑1 (MQ=255)
                                                                              gcGGCAAATTAAACGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                        <  2:1076105/149‑1 (MQ=255)
                                                                                 gCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCATATTCCGGGCTAc                                                                     <  2:280551/150‑1 (MQ=255)
                                                                                      ttAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGGCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACt                                                                <  1:1432519/150‑1 (MQ=255)
                                                                                         aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATTCCTGTCTGTCATGGTGCAACGTAAAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGATCCGATTACGGGCTAAATACTGGc                                                             >  2:1242495/1‑150 (MQ=255)
                                                                                         aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGc                                                             <  2:29020/150‑1 (MQ=255)
                                                                                               tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                       <  2:127227/150‑1 (MQ=255)
                                                                                                 aTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttc                                                     <  1:330515/150‑1 (MQ=255)
                                                                                                    ttGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttcttc                                                  <  2:1401923/150‑1 (MQ=255)
                                                                                                    ttGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttcttc                                                  <  1:1110112/150‑1 (MQ=255)
                                                                                                     tGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTca                                                 <  2:392955/150‑1 (MQ=255)
                                                                                                        gcCCAACTCTGCCATTCATCCGTCCCGGAGTCGTGGCTCCATGCCTGTCTGTCATGGAGGAACGTACAGTGACACAGGTTAATTACCTTGTTGGTTAAATCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcc                                              <  1:1223270/150‑1 (MQ=255)
                                                                                                            aaCTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCa                                          <  1:437764/150‑1 (MQ=255)
                                                                                                               tATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCTGTATGGCAAGGAGCCACGTACAGTGCCCCAAGTAAATTACCGTTTTTGTTTAAGCATGGCTTTGAGCGTATTCAGGGCGACATACTGTCAGGATTTATTaatacataacaa                                       >  2:912835/1‑145 (MQ=255)
                                                                                                                 tGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAg                                     <  1:832487/150‑1 (MQ=255)
                                                                                                                   ccATTCCTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtg                                   <  2:209213/150‑1 (MQ=255)
                                                                                                                        aaTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgag                              <  2:1348507/149‑1 (MQ=255)
                                                                                                                         aTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgagt                             <  2:6322/150‑1 (MQ=255)
                                                                                                                          tCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGTAGGATTTCTTCATCCATCAGAAAGTGAtgagtg                            <  2:232869/150‑1 (MQ=255)
                                                                                                                                   gAGGCGTTGCTCCATCCCTGTCTGTCATGGAGCAACGTACAGTGCCACAGGTTAATTACCGTGTAGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGGTGCGCt                   <  2:339815/150‑1 (MQ=255)
                                                                                                                                    aGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTg                  <  1:1437515/150‑1 (MQ=255)
                                                                                                                                      tCGTTGCTCCATGCCTGACTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGAc                <  2:827819/150‑1 (MQ=255)
                                                                                                                                       cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg               >  1:462468/1‑150 (MQ=255)
                                                                                                                                       cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg               >  2:370198/1‑150 (MQ=255)
                                                                                                                                         ttGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc             <  1:552228/150‑1 (MQ=255)
                                                                                                                                         ttGCTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc             >  2:29976/1‑150 (MQ=255)
                                                                                                                                           gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc           >  1:1193442/1‑150 (MQ=255)
                                                                                                                                           gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc           >  1:1085421/1‑150 (MQ=255)
                                                                                                                                            cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca          <  2:1506956/150‑1 (MQ=255)
                                                                                                                                            cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGAGTTGGTTAAAGAATGACTTTGAGCGTATTCCGGGCTACATACTGGAAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGATGCGCTGACGGCGca          >  1:667304/1‑150 (MQ=255)
                                                                                                                                            cTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca          >  1:1604082/1‑150 (MQ=255)
                                                                                                                                               cATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatca       <  1:1132101/150‑1 (MQ=255)
                                                                                                                                                  gCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTACCGTGTTGGGTAAAGCCTGTCTTTGAGCGTCTTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGCAAGTGCTGAGTGAGTTGCGCCGTCAGCGcaactcac    <  1:784712/150‑7 (MQ=255)
                                                                                                                                                  gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCAtcatca    >  1:1124170/1‑150 (MQ=255)
                                                                                                                                                  gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGGTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcat    <  1:130901/150‑7 (MQ=255)
                                                                                                                                                   ccTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcact   <  1:1729434/150‑8 (MQ=255)
                                                                                                                                                    ctgtctgtCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCaaa  >  1:332001/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAA  >  NC_000913/2476867‑2477164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: