Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,477,018 T→A 15.8% R204S (AGA→AGT) ‡ dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,0180TA15.8% 140.9 / 22.9 77R204S (AGA→AGT) ‡dsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (18/44);  new base A (2/10);  total (22/55)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01

TCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTA  >  NC_000913/2476873‑2477167
                                                                                                                                                 |                                                                                                                                                      
tCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgtctgt                                                                                                                                                    <  1:375055/150‑1 (MQ=255)
  gCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCa                                                                                                                                                  <  1:1447864/150‑1 (MQ=255)
   ccACCCCATTATGATTAATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGTAGATGTCGGCAAATTAACCGCATAATTCTGCGCCCAACTATGCCATTCATCCGAACCGGAGTCGTTGCTCCATGCCTGTCTGTCAt                                                                                                                                                 <  2:70203/150‑1 (MQ=255)
   ccACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGCTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCctgtcggtgca                                                                                                                                                 >  1:111513/1‑147 (MQ=255)
      ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCTTACCGGAGTCGTTGATCCAGGGCTGGCTGTGAttgg                                                                                                                                              >  2:301556/1‑147 (MQ=255)
      ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGa                                                                                                                                              <  2:846675/150‑1 (MQ=255)
      ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGa                                                                                                                                              <  2:375047/150‑1 (MQ=255)
          aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                          <  1:1634749/150‑1 (MQ=255)
          aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                          <  2:479662/150‑1 (MQ=255)
           atatGATTCATCGCGGCAATAACAGCTAAATCAGATCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                         <  1:1703771/150‑1 (MQ=255)
           atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCCGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                         <  2:1157004/150‑1 (MQ=255)
           atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAAGCGCATATTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAc                                                                                                                                         <  1:208638/150‑1 (MQ=255)
           atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                         <  1:979411/150‑1 (MQ=255)
           atatGAATCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGACAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCGCCATGACAGACAGGCATGGAGCAAc                                                                                                                                         <  2:127759/150‑1 (MQ=255)
            tatGATTCATAGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAACg                                                                                                                                        <  2:821138/150‑1 (MQ=255)
              tGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTa                                                                                                                                      <  1:480599/150‑1 (MQ=255)
                   cATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGTGCAACGTACAGTg                                                                                                                                 <  1:873292/150‑1 (MQ=255)
                     tCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCTGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGac                                                                                                                               <  1:1012691/150‑1 (MQ=255)
                         ggCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAg                                                                                                                          <  2:1553453/150‑1 (MQ=255)
                           cAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGACTGTCATGGAGCAACGTACAGTGACACAGGt                                                                                                                        <  1:301556/150‑1 (MQ=255)
                              tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTaa                                                                                                                     <  2:1536714/150‑1 (MQ=255)
                               aaCAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCACCCAGGCTGTCGGTACTGTATCAACCTTCAATGACACATGTTTAt                                                                                                                    >  2:1177468/1‑150 (MQ=255)
                                   gCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCAGTCTGTCAATGAGCAACCTAAAGTGACACAGGGTTAATAAc                                                                                                                 >  1:6322/1‑150 (MQ=255)
                                         tCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTg                                                                                                          <  1:767576/150‑1 (MQ=255)
                                          cAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTgg                                                                                                         <  2:1363879/150‑1 (MQ=255)
                                          cAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCAGGCAGTCATGGAGCAACGTACAAGGACACCGGGTAATTACCGGGTTgg                                                                                                         >  1:1175469/1‑150 (MQ=255)
                                           aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCAACAAGCCTGTCTGTCATGGAGCAACGTTCATTGACACAGGTTTATTACCCTGTTggg                                                                                                        >  2:1132101/1‑149 (MQ=255)
                                           aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTTCACCAATCCAGTATGTAAAGGTGCAACGGTAAGGTCCACCGGTTAATTAACctgtgtgg                                                                                                        >  2:130901/1‑146 (MQ=255)
                                            gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGGCTGTCTGTGATGGAGCAACGTACAGTGGCACAGGGTTATTAACGtggtgggt                                                                                                       >  1:1137557/1‑148 (MQ=255)
                                            gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTAACGTGTTGGtt                                                                                                       >  2:916011/1‑150 (MQ=255)
                                            gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                       <  1:1506145/150‑1 (MQ=255)
                                            aaaCGATCAAAGCCAATTCCAGACCATGTCGGCAAATAAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                       <  2:1570206/149‑1 (MQ=255)
                                              aCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCAATCATCCGTACCGGAGTCGTTGCACCATGGCAGTCTGTCATGGTGCAACGTACAGTGACACAGGTTTAATACCGGGTTGGTTaa                                                                                                     >  1:224075/1‑150 (MQ=255)
                                                gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCGGTCTGTCATGGTGCAACGTACAGTGTCCCCGGGTTATTACCGTGTTGGGTAAAg                                                                                                   >  1:243565/1‑150 (MQ=255)
                                                    aaaGACAATTCCAGAAGTTGTCGGCAAATTAACCGATTAATGTTGCGCCCATCTATGCCATTCATACGTTCGGGTGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACTGTGACACAGGTTAATTACCGTGTTGGTTATAGGATg                                                                                               <  2:104537/150‑1 (MQ=255)
                                                     aaGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGt                                                                                              <  1:664356/150‑1 (MQ=255)
                                                        ccAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAAGTACAGTGACACAGGTTAATTAACGTGTTGGTTTAAGCATGTcat                                                                                           >  2:18632/1‑148 (MQ=255)
                                                            ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                       <  2:1170195/150‑1 (MQ=255)
                                                            ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                       <  1:873322/150‑1 (MQ=255)
                                                              ccAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCg                                                                                     <  2:957323/150‑1 (MQ=255)
                                                               cAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGt                                                                                    <  2:367886/150‑1 (MQ=255)
                                                                      tGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGATCAAAGCCTGTCTGTCATGGAGGAAAGTACAGTGACAAAGGGTTATTAAAGTGTTGGGTAAAGCATGTATTTGAGCGttatcagg                                                                             >  1:1108807/1‑143 (MQ=255)
                                                                        tCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                           <  2:421862/150‑1 (MQ=255)
                                                                        gcGGCAAATTACCCGCATAATGGTGCGCCCAACTATGCCATTCTTCCGTACCGGAGTCGTTGCTGCATGCCTGGCTGTCAAGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                           <  1:366750/149‑1 (MQ=255)
                                                                        gcGGCAAATTAAACGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                           <  2:1076105/149‑1 (MQ=255)
                                                                           gCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCATATTCCGGGCTAc                                                                        <  2:280551/150‑1 (MQ=255)
                                                                                ttAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGGCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACt                                                                   <  1:1432519/150‑1 (MQ=255)
                                                                                   aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATTCCTGTCTGTCATGGTGCAACGTAAAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGATCCGATTACGGGCTAAATACTGGc                                                                >  2:1242495/1‑150 (MQ=255)
                                                                                   aCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGc                                                                <  2:29020/150‑1 (MQ=255)
                                                                                         tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                          <  2:127227/150‑1 (MQ=255)
                                                                                           aTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttc                                                        <  1:330515/150‑1 (MQ=255)
                                                                                              ttGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttcttc                                                     <  1:1110112/150‑1 (MQ=255)
                                                                                              ttGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttcttc                                                     <  2:1401923/150‑1 (MQ=255)
                                                                                               tGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTca                                                    <  2:392955/150‑1 (MQ=255)
                                                                                                  gcCCAACTCTGCCATTCATCCGTCCCGGAGTCGTGGCTCCATGCCTGTCTGTCATGGAGGAACGTACAGTGACACAGGTTAATTACCTTGTTGGTTAAATCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcc                                                 <  1:1223270/150‑1 (MQ=255)
                                                                                                      aaCTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCa                                             <  1:437764/150‑1 (MQ=255)
                                                                                                         tATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCTGTATGGCAAGGAGCCACGTACAGTGCCCCAAGTAAATTACCGTTTTTGTTTAAGCATGGCTTTGAGCGTATTCAGGGCGACATACTGTCAGGATTTATTaatacataacaa                                          >  2:912835/1‑145 (MQ=255)
                                                                                                           tGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAg                                        <  1:832487/150‑1 (MQ=255)
                                                                                                             ccATTCCTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtg                                      <  2:209213/150‑1 (MQ=255)
                                                                                                                  aaTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgag                                 <  2:1348507/149‑1 (MQ=255)
                                                                                                                   aTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgagt                                <  2:6322/150‑1 (MQ=255)
                                                                                                                    tCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGTAGGATTTCTTCATCCATCAGAAAGTGAtgagtg                               <  2:232869/150‑1 (MQ=255)
                                                                                                                             gAGGCGTTGCTCCATCCCTGTCTGTCATGGAGCAACGTACAGTGCCACAGGTTAATTACCGTGTAGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGGTGCGCt                      <  2:339815/150‑1 (MQ=255)
                                                                                                                              aGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTg                     <  1:1437515/150‑1 (MQ=255)
                                                                                                                                tCGTTGCTCCATGCCTGACTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGAc                   <  2:827819/150‑1 (MQ=255)
                                                                                                                                 cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg                  >  1:462468/1‑150 (MQ=255)
                                                                                                                                 cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg                  >  2:370198/1‑150 (MQ=255)
                                                                                                                                   ttGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc                <  1:552228/150‑1 (MQ=255)
                                                                                                                                   ttGCTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgc                >  2:29976/1‑150 (MQ=255)
                                                                                                                                     gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc              >  1:1193442/1‑150 (MQ=255)
                                                                                                                                     gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc              >  1:1085421/1‑150 (MQ=255)
                                                                                                                                      cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca             <  2:1506956/150‑1 (MQ=255)
                                                                                                                                      cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGAGTTGGTTAAAGAATGACTTTGAGCGTATTCCGGGCTACATACTGGAAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGATGCGCTGACGGCGca             >  1:667304/1‑150 (MQ=255)
                                                                                                                                      cTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca             >  1:1604082/1‑150 (MQ=255)
                                                                                                                                         cATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatca          <  1:1132101/150‑1 (MQ=255)
                                                                                                                                            gCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTACCGTGTTGGGTAAAGCCTGTCTTTGAGCGTCTTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGCAAGTGCTGAGTGAGTTGCGCCGTCAGCGcaactcac       <  1:784712/150‑7 (MQ=255)
                                                                                                                                            gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCAtcatca       >  1:1124170/1‑150 (MQ=255)
                                                                                                                                            gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGGTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcat       <  1:130901/150‑7 (MQ=255)
                                                                                                                                             ccTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcact      <  1:1729434/150‑8 (MQ=255)
                                                                                                                                              ctgtctgtCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCaaa     >  1:332001/1‑150 (MQ=255)
                                                                                                                                                gtctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGt   <  1:1507229/150‑1 (MQ=255)
                                                                                                                                                 tctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTa  <  1:1664849/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
TCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTA  >  NC_000913/2476873‑2477167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: