Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 292,302 C→G 7.0% intergenic (+71/+20) yagJ → / ← yagK CP4‑6 prophage; uncharacterized protein;Phage or Prophage Related/CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913292,3020CG 7.0% 411.7 / 17.3 129intergenic (+71/+20)yagJ/yagKCP4‑6 prophage; uncharacterized protein;Phage or Prophage Related/CP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base C (60/60);  new base G (4/5);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 131 total aligned reads displayed.

ATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTG  >  NC_000913/292155‑292451
                                                                                                                                                    |                                                                                                                                                     
ttgtgCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAAAGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATAGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATCAGGCCTTCTCACTg                                                                                                                                                     <  1:1042766/149‑1 (MQ=255)
aTGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTTGAAACAATACAAATAAGGCCTTCTCACt                                                                                                                                                      <  2:394648/150‑1 (MQ=255)
aTGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTg                                                                                                                                                     <  2:1631783/150‑1 (MQ=255)
aTGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTg                                                                                                                                                     >  1:680804/1‑150 (MQ=255)
  gtgCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATACGGCCTTCGCACTgag                                                                                                                                                   >  2:887140/1‑150 (MQ=255)
  gtgCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTgag                                                                                                                                                   >  1:1073098/1‑150 (MQ=255)
  gtgCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTgag                                                                                                                                                   <  1:686427/150‑1 (MQ=255)
    gCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGaa                                                                                                                                                 >  1:622839/1‑150 (MQ=255)
    gCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGaa                                                                                                                                                 >  1:455466/1‑150 (MQ=255)
    gCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAAAGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTTCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACATTACAAATAAGGCCTTCTCACTGAGaa                                                                                                                                                 <  1:1209575/150‑1 (MQ=255)
     cAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAg                                                                                                                                                <  1:1683921/150‑1 (MQ=255)
     cAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGACTTCTCACTGAGAAg                                                                                                                                                >  2:583941/1‑150 (MQ=255)
       ggATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGc                                                                                                                                              >  2:1329277/1‑150 (MQ=255)
        gATGCTGTTTCTACCGCTTAAAATGCAGAAACGATATTTAGTAGCGGACATGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCAGTGAGAAGGcc                                                                                                                                             <  2:90376/150‑1 (MQ=255)
         aTGCTGTTGCTACCGCTGAAAATGCATAAACTTTATTTAGTAGAGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCAGTGAGAAGGcct                                                                                                                                            <  1:926056/150‑2 (MQ=255)
         aTGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCAGTGAGAAGGcct                                                                                                                                            <  1:1326204/150‑2 (MQ=255)
         aTGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCa                                                                                                                                            >  1:653126/1‑150 (MQ=255)
          tGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACTAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCAGTGAGAAGGcctt                                                                                                                                           <  2:941240/150‑3 (MQ=255)
           gCTGTTGCTACCGCTGAAAATGCAGAAACTTTATTTAGTAGCGTACTTGATGATGAATATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGATACAATACAAATAAGGCCTTCTCACTGAGAAGGCCAtt                                                                                                                                          <  1:254152/150‑1 (MQ=255)
           gCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCAtt                                                                                                                                          <  2:847178/150‑1 (MQ=255)
             tGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTTTCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTAc                                                                                                                                        <  1:555403/150‑1 (MQ=255)
                tGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGa                                                                                                                                     <  1:171770/150‑1 (MQ=255)
                 gCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGTACTTGCTGATGACTATCAGTAATCAATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGATAAGGCCATTACCGAc                                                                                                                                    <  2:864294/150‑1 (MQ=255)
                   tACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACtt                                                                                                                                  >  2:799403/1‑150 (MQ=255)
                   aaCCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGAAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACtt                                                                                                                                  <  2:846805/149‑1 (MQ=255)
                     ccGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTAc                                                                                                                                >  2:819212/1‑150 (MQ=255)
                       gCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAg                                                                                                                              >  2:1541671/1‑150 (MQ=255)
                       gCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCAATTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAg                                                                                                                              >  1:931699/1‑150 (MQ=255)
                        cTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGt                                                                                                                             <  1:1642196/150‑1 (MQ=255)
                         tGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGtt                                                                                                                            <  1:571250/150‑1 (MQ=255)
                           aaaaTGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCg                                                                                                                          >  2:1621158/1‑150 (MQ=255)
                           aaaaTGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAAAACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGATCg                                                                                                                          <  2:1067298/150‑1 (MQ=255)
                                 cAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTgg                                                                                                                    >  1:1541843/1‑150 (MQ=255)
                                    aaaCGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCAGTg                                                                                                                                                     >  2:419740/1‑114 (MQ=255)
                                         ttATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTATTAATTTTTTGAAACAATACAAATTATGCCTTCTCACTGAGTAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGcc                                                                                                            <  2:1013605/150‑1 (MQ=255)
                                             ttAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAg                                                                                                        <  2:511450/150‑1 (MQ=255)
                                                  agCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTAACAGTTCGATTTGGCTACAGCCGAAGTTACg                                                                                                   >  1:1047287/1‑150 (MQ=255)
                                                   gCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACgg                                                                                                  <  2:373594/150‑1 (MQ=255)
                                                      gACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAGACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGtct                                                                                               <  1:1296252/150‑1 (MQ=255)
                                                       aCTTGCTGCTGACTATCAGTAATCTATAGACAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACACTTCGATTTGGCTACAGACGAAGTTACGGtctc                                                                                              >  1:688905/1‑150 (MQ=255)
                                                        cTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGTCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTcc                                                                                             >  2:146296/1‑150 (MQ=255)
                                                        cTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTcc                                                                                             <  1:411645/150‑1 (MQ=255)
                                                        cTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTAAAGTTCGATTTGGCTACATCCGAAGTTACTGTCTcc                                                                                             >  2:1286722/1‑150 (MQ=255)
                                                          tGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCtt                                                                                           >  2:883575/1‑150 (MQ=255)
                                                           gCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTc                                                                                          <  1:750551/150‑1 (MQ=255)
                                                            cTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCa                                                                                         >  2:1295762/1‑150 (MQ=255)
                                                              gatgaCTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACTAATAAGTCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCAcc                                                                                       <  2:719294/150‑1 (MQ=255)
                                                              gatgaCTATAAGTAATCTATAGAAATTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCAcc                                                                                       <  2:1718436/150‑1 (MQ=255)
                                                                 gaCTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCaaa                                                                                    >  2:146726/1‑150 (MQ=255)
                                                                  aCTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCTAac                                                                                   >  2:750178/1‑150 (MQ=255)
                                                                    tATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAccac                                                                                 >  2:519669/1‑150 (MQ=255)
                                                                       cAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACttt                                                                              <  2:712587/150‑1 (MQ=255)
                                                                        aGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTg                                                                             <  2:1433607/150‑1 (MQ=255)
                                                                         gTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGc                                                                            <  2:1143975/150‑1 (MQ=255)
                                                                                ataGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAAc                                                                     >  2:338963/1‑150 (MQ=255)
                                                                                 taGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACt                                                                    >  1:36645/1‑150 (MQ=255)
                                                                                   taaaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACttt                                                                  <  1:1252629/149‑1 (MQ=255)
                                                                                   gAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACttt                                                                  >  2:1282167/1‑150 (MQ=255)
                                                                                    aaaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTg                                                                 <  1:12541/150‑1 (MQ=255)
                                                                                    aaaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTg                                                                 <  1:176230/150‑1 (MQ=255)
                                                                                    aaaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAAAACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTg                                                                 <  2:319095/150‑1 (MQ=255)
                                                                                     aaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTgg                                                                <  1:1486324/150‑1 (MQ=255)
                                                                                     aaaTTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTgg                                                                <  1:1526582/150‑1 (MQ=255)
                                                                                           cGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGt                                                          >  1:1370101/1‑150 (MQ=255)
                                                                                                cGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTcc                                                     <  2:1709704/150‑1 (MQ=255)
                                                                                                  aaaCTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAg                                                   <  1:312071/150‑1 (MQ=255)
                                                                                                    aCTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTc                                                 <  2:1151374/150‑1 (MQ=255)
                                                                                                       gCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCggg                                              <  2:382514/150‑1 (MQ=255)
                                                                                                        cATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCTAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGt                                             <  1:750178/150‑1 (MQ=255)
                                                                                                        cATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGt                                             >  2:1523695/1‑150 (MQ=255)
                                                                                                        cATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGt                                             <  1:764358/150‑1 (MQ=255)
                                                                                                         aTTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGtt                                            <  1:1528489/150‑1 (MQ=255)
                                                                                                               gTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAgc                                      >  2:857155/1‑150 (MQ=255)
                                                                                                               gTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAgc                                      >  2:376867/1‑150 (MQ=255)
                                                                                                               gTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAgc                                      >  2:977453/1‑150 (MQ=255)
                                                                                                                tAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAgcg                                     >  1:1277957/1‑150 (MQ=255)
                                                                                                                 aaTTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCTGGTTAACAgcgc                                    >  1:615023/1‑150 (MQ=255)
                                                                                                                 aaTTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAgcgc                                    >  1:1098905/1‑150 (MQ=255)
                                                                                                                     tttttGAAACAATACAAATAAGGCATTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGTTCTCATTAACCAAACACTTTGCTTTCCACTTTGGTCAGGTAGTCAAGGCGGGTTAACAGCGCCTg                                 >  2:31864/1‑150 (MQ=255)
                                                                                                                         tGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAg                             <  1:1300416/150‑1 (MQ=255)
                                                                                                                         tGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAg                             >  2:1468476/1‑150 (MQ=255)
                                                                                                                               aaTACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTATCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTctgc                       >  2:1454251/1‑150 (MQ=255)
                                                                                                                               aaTACAAATAAGGCCTTCTCACTGAGAAGGCCATTAAAGACTTACAGTTAGATATCGCAAAATCCGAAAATACTATATACTTCACAAAACACTTTGCTTTCAAATATGGTAAGGAAGTCAAGTCGTGTTAACAGCGCCTGGTAGttatta                       >  2:330764/1‑146 (MQ=255)
                                                                                                                                aTACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCACACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTctgct                      >  1:1509152/1‑150 (MQ=255)
                                                                                                                                aTACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTctgct                      <  2:1025951/150‑1 (MQ=255)
                                                                                                                                    cggtAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCGTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGaa                  >  2:1152749/4‑150 (MQ=255)
                                                                                                                                     aaTAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAg                 >  1:909506/1‑150 (MQ=255)
                                                                                                                                     aaTAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAg                 >  1:445198/1‑150 (MQ=255)
                                                                                                                                         tggCCTTCTCAGTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAg             >  1:1199030/2‑150 (MQ=255)
                                                                                                                                         tggCCTTCTCAGTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAg             >  1:1390400/2‑150 (MQ=255)
                                                                                                                                            ccTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTAc          >  1:1015955/1‑150 (MQ=255)
                                                                                                                                            ccTTCTCACTGAGAACGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGAAGTCCAGTCGGGATAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTAc          >  1:357864/1‑150 (MQ=255)
                                                                                                                                               tctcACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTAt       >  1:1681097/1‑150 (MQ=255)
                                                                                                                                                  cACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTgg    >  1:583856/1‑150 (MQ=255)
                                                                                                                                                  cACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTgg    <  2:622839/150‑1 (MQ=255)
                                                                                                                                                  cACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTgg    <  2:421189/150‑1 (MQ=255)
                                                                                                                                                   aCTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAATCACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTgtt   >  1:805768/1‑148 (MQ=255)
                                                                                                                                                   aCTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGgt   >  1:424997/1‑150 (MQ=255)
                                                                                                                                                   aCTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGgt   <  2:1710873/150‑1 (MQ=255)
                                                                                                                                                   aCTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACATTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGgt   >  2:554264/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAGCCGAAGTTACGGTCTCCTTCACCAAACACTTTGCTTTCAACTTTGGTCAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTG  >  NC_000913/292155‑292451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: