BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,477,877	C→A	5.4%	L11M (CTG→ATG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,877	0	C	A	5.4%	296.1 / 4.2	100	L11M (CTG→ATG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand): ref base C (50/44); new base A (4/2); total (54/46)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGC  >  NC_000913/2477731‑2478026

tGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACgctgc                                                                                                                                                    >  1:439451/1‑150 (MQ=255)

tGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAGTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCCCTCTCAAATCT‑GGGTTGTGAGCACgctgc                                                                                                                                                    >  2:311524/1‑150 (MQ=255)

     aTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATc                                                                                                                                               <  2:695897/150‑1 (MQ=255)

      tCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCa                                                                                                                                              <  1:1391833/150‑1 (MQ=255)

            cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCg                                                                                                                                        >  1:990159/1‑150 (MQ=255)

            cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCg                                                                                                                                        <  1:1700123/150‑1 (MQ=255)

                gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCACATCT‑GGGTTGTGAGCACGCTGGTTATCAGCAACGTGtt                                                                                                                                    >  2:313062/1‑150 (MQ=255)

                gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGtt                                                                                                                                    <  2:729166/150‑1 (MQ=255)

                gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGtt                                                                                                                                    <  1:531146/150‑1 (MQ=255)

                gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGtt                                                                                                                                    >  1:143386/1‑150 (MQ=255)

                   gCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAAt                                                                                                                                 <  1:412406/150‑1 (MQ=255)

                    cAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCTTTGTTTTTTTGTCCAATATCATCAGGTTAATAACAGGGGAAGGTGAGATATGACATCTCAAATCT‑GCGTTGTGAGCACGCTGCTTATCAGCATCCTGATAAtt                                                                                                                                >  2:838583/1‑150 (MQ=255)

                     aaTTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATAACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAAttt                                                                                                                               >  1:645753/1‑149 (MQ=255)

                     aaTTTACCTCATGCGAAAGGCAAATTTTATCGTATGGCAGCCTCCCTTGTTTTTTTGTCCAATATCATCAGGGTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTGGTGAGCACGCTGCTTATCAGCATCGTGTTAATTg                                                                                                                               <  2:1596245/150‑1 (MQ=255)

                           ccTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGa                                                                                                                         >  2:1111079/1‑150 (MQ=255)

                             tCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGAcc                                                                                                                       >  1:1628099/1‑150 (MQ=255)

                                 gtgAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTAAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCg                                                                                                                   <  2:1010486/150‑1 (MQ=255)

                                  tgATAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGt                                                                                                                  <  1:1443313/150‑1 (MQ=255)

                                   gAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTg                                                                                                                 <  1:950387/150‑1 (MQ=255)

                                   gAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTg                                                                                                                 >  2:528262/1‑150 (MQ=255)

                                    aaaGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGa                                                                                                                <  2:1592244/150‑1 (MQ=255)

                                      aGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAg                                                                                                              >  1:497726/1‑150 (MQ=255)

                                       ggCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGt                                                                                                             >  1:690951/1‑150 (MQ=255)

                                        gCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                                                            >  1:216036/1‑150 (MQ=255)

                                        gCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                                                            >  2:1340905/1‑150 (MQ=255)

                                        gCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCAAAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGAACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                                                            >  2:6272/1‑150 (MQ=255)

                                                tATCGTTTGTCAGCATGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTcc                                                                                                    <  2:1394512/150‑1 (MQ=255)

                                                  tCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                                                                  <  1:1338792/150‑1 (MQ=255)

                                                  tCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                                                                  >  1:1379958/1‑150 (MQ=255)

                                                  tCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                                                                  <  2:143386/150‑1 (MQ=255)

                                                       tGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTTATCACAGGGGAAGGTGAGATATGGACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTTTCAGCATCGTGTTAATTGTTCTGACCATCGTGAAGTTCTAATTCCACCCGtt                                                                                             >  1:860014/1‑150 (MQ=255)

                                                         tCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTc                                                                                           >  1:1706639/1‑150 (MQ=255)

                                                            gCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGATGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTgg                                                                                        >  1:921517/1‑150 (MQ=255)

                                                              cTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGgcg                                                                                      <  1:998461/150‑1 (MQ=255)

                                                                gCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTTATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCt                                                                                    >  1:1433944/1‑150 (MQ=255)

                                                                 cgatgttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGAACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTg                                                                                   >  2:1150512/4‑150 (MQ=255)

                                                                   ttgttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACAATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGtt                                                                                 >  2:883928/1‑150 (MQ=255)

                                                                     gttTTTTTGTCGAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGc                                                                               <  2:1488201/150‑1 (MQ=255)

                                                                         ttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGc                                                                           <  1:920566/150‑1 (MQ=255)

                                                                          tttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGcc                                                                          <  2:1601213/150‑1 (MQ=255)

                                                                             gTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGc                                                                       <  2:1628099/150‑1 (MQ=255)

                                                                              tCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGct                                                                      <  2:235291/150‑1 (MQ=255)

                                                                              tCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGct                                                                      <  2:439451/150‑1 (MQ=255)

                                                                               ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                                                                     >  1:787259/1‑150 (MQ=255)

                                                                               ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                                                                     >  2:415895/1‑150 (MQ=255)

                                                                               ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                                                                     >  2:632692/1‑150 (MQ=255)

                                                                                cAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGcttc                                                                    <  2:161900/150‑1 (MQ=255)

                                                                                 aaTATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGcttct                                                                   >  2:311147/1‑150 (MQ=255)

                                                                                    atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                <  2:1357773/150‑1 (MQ=255)

                                                                                    atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                <  2:473012/150‑1 (MQ=255)

                                                                                    atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                <  2:645753/150‑1 (MQ=255)

                                                                                    atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                <  2:954364/150‑1 (MQ=255)

                                                                                    atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                <  1:1541926/150‑1 (MQ=255)

                                                                                      tatcaGGTTTATCACTGGGGAAGGTGAGATTTGCACTCTCAAATCT‑GGGTTGTGAGCACTCTGCTTATCAGCATCGTGTTAATTGTACTGAACATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTg                                                              <  2:156194/149‑1 (MQ=255)

                                                                                      catcaGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAAACT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTTGCGCTGTTGCTGGCAAGATTCTTCGTg                                                              >  2:613280/1‑150 (MQ=255)

                                                                                       atcaGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTgg                                                             >  2:11300/1‑150 (MQ=255)

                                                                                         caGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCCTCGTGGGa                                                           >  2:743436/1‑150 (MQ=255)

                                                                                          aGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCGGGCCAGCTTCTTCGTGGGaa                                                          <  1:311524/150‑1 (MQ=255)

                                                                                              tAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgat                                                      >  1:442614/1‑150 (MQ=255)

                                                                                               aaTCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGCACgatg                                                     >  2:855624/1‑150 (MQ=255)

                                                                                                 tCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgatgat                                                   <  1:604866/150‑1 (MQ=255)

                                                                                                     aGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGc                                               >  2:1028165/1‑150 (MQ=255)

                                                                                                      ggggAAGGTGAGATATGCACTCTCAAATCT‑GGGGTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCa                                              <  2:1531815/150‑1 (MQ=255)

                                                                                                          aaGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTTCTGACCATCGTGAAGTTCTAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATggg                                          <  1:1150512/150‑1 (MQ=255)

                                                                                                           aGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATgggg                                         <  2:527912/150‑1 (MQ=255)

                                                                                                           aGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATgggg                                         <  1:1245236/150‑1 (MQ=255)

                                                                                                             gTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGcc                                       >  1:552432/1‑150 (MQ=255)

                                                                                                              tGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCa                                      >  1:853731/1‑150 (MQ=255)

                                                                                                                  atatGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTgg                                  >  2:1704703/1‑150 (MQ=255)

                                                                                                                  atatGCACTCTCAAATCT‑GGGTGGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTgg                                  <  2:1323768/150‑1 (MQ=255)

                                                                                                                    atGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGat                                >  2:1527719/1‑150 (MQ=255)

                                                                                                                    atGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGat                                >  2:785193/1‑150 (MQ=255)

                                                                                                                     tGCACTCTCAAATCTGGGGTTGTGAGCCCGCTGCTTATCAGCATCGTGTTAATTGTACTGACCAGCGTGAAGTGCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGat                                <  2:1176610/150‑1 (MQ=255)

                                                                                                                      gCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGATGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGatat                              >  1:250983/1‑150 (MQ=255)

                                                                                                                       cACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATg                             >  1:1180164/1‑150 (MQ=255)

                                                                                                                        aCTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATgg                            <  1:1068572/150‑1 (MQ=255)

                                                                                                                         ctctCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGt                           >  2:1054565/1‑150 (MQ=255)

                                                                                                                         ctctCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGATTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGt                           >  1:692766/1‑150 (MQ=255)

                                                                                                                          tctcAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTACTTCGTGGCAACGATGATGGGCATGGGGCCACTGGATATGGTa                          >  2:804948/1‑150 (MQ=255)

                                                                                                                               aactcggggTTGTGAGCCCGCAGCTTATCAGCATCGTGTTAATTCTACTGACCCTCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCGGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATg                      <  1:854573/144‑1 (MQ=255)

                                                                                                                                  cT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTAt                  >  1:1672513/1‑150 (MQ=255)

                                                                                                                                  cT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGATGCTGGCCAGATTCTTAGTGGGAACGATGATTGGCATGGGGACACTGGATATGGTAAATGCTAt                  >  2:1045719/1‑150 (MQ=255)

                                                                                                                                     gggTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTg                >  2:1198558/1‑150 (MQ=255)

                                                                                                                                      ggTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGa               >  1:1292593/1‑150 (MQ=255)

                                                                                                                                       gTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGaa              >  1:1265591/1‑150 (MQ=255)

                                                                                                                                       gTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCAAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGaa              >  1:1070766/1‑150 (MQ=255)

                                                                                                                                         tgtgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAg            <  1:528262/150‑1 (MQ=255)

                                                                                                                                          gtgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGt           <  1:1340905/150‑1 (MQ=255)

                                                                                                                                           ttaacACGATGCTGATAAGCAGCGTGGTAATTGTACTGACCATCGTGAAGTTCAAATTCCACACGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGAGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg          >  1:899378/5‑150 (MQ=255)

                                                                                                                                           tgAGCACGCTGCTTATCAGCATCGTGTTAATTGTCCTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg          <  1:360313/150‑1 (MQ=255)

                                                                                                                                           tgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg          >  1:1462973/1‑150 (MQ=255)

                                                                                                                                             cgCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACGATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGTTAAATGCTATTGAAAGTTGa        <  1:6272/149‑1 (MQ=255)

                                                                                                                                               cACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATAAGGTAAATGCTATTGAAAGTGGAAt      >  1:932018/1‑150 (MQ=255)

                                                                                                                                               cACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg          >  2:1379290/1‑146 (MQ=255)

                                                                                                                                               cACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg          <  1:1379290/146‑1 (MQ=255)

                                                                                                                                               cACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAAt      >  1:320982/1‑150 (MQ=255)

                                                                                                                                                 cGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTg    >  1:164607/1‑150 (MQ=255)

                                                                                                                                                  gctgctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTgg   <  1:1011993/150‑1 (MQ=255)

                                                                                                                                                   ctgctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGc  <  2:818741/150‑1 (MQ=255)

                                                                                                                                                   ctgctTATCAGCATCGTGTTAATGGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTCCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGc  <  2:818738/150‑1 (MQ=255)

TGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGC  >  NC_000913/2477731‑2478026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`