BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,477,890	T→G	5.3%	I15S (ATC→AGC)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,890	0	T	G	5.3%	340.3 / 7.2	113	I15S (ATC→AGC)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand): ref base T (54/52); new base G (4/2); total (59/54)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 114 total aligned reads displayed.

CATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGT  >  NC_000913/2477743‑2478039

cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCg                                                                                                                                                     <  1:1700123/150‑1 (MQ=255)

cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCg                                                                                                                                                     >  1:990159/1‑150 (MQ=255)

   tGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGATGCTGATAAGCAGCgtgc                                                                                                                                                  <  1:1670583/150‑2 (MQ=255)

    gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCACATCT‑GGGTTGTGAGCACGCTGGTTATCAGCAACGTGtt                                                                                                                                                 >  2:313062/1‑150 (MQ=255)

    gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGtt                                                                                                                                                 <  1:531146/150‑1 (MQ=255)

    gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGtt                                                                                                                                                 >  1:143386/1‑150 (MQ=255)

    gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGtt                                                                                                                                                 <  2:729166/150‑1 (MQ=255)

       gCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAAt                                                                                                                                              <  1:412406/150‑1 (MQ=255)

        cAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCTTTGTTTTTTTGTCCAATATCATCAGGTTAATAACAGGGGAAGGTGAGATATGACATCTCAAATCT‑GCGTTGTGAGCACGCTGCTTATCAGCATCCTGATAAtt                                                                                                                                             >  2:838583/1‑150 (MQ=255)

         aaTTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATAACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAAttt                                                                                                                                            >  1:645753/1‑149 (MQ=255)

         aaTTTACCTCATGCGAAAGGCAAATTTTATCGTATGGCAGCCTCCCTTGTTTTTTTGTCCAATATCATCAGGGTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTGGTGAGCACGCTGCTTATCAGCATCGTGTTAATTg                                                                                                                                            <  2:1596245/150‑1 (MQ=255)

               ccTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGa                                                                                                                                      >  2:1111079/1‑150 (MQ=255)

                 tCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGAcc                                                                                                                                    >  1:1628099/1‑150 (MQ=255)

                     gtgAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTAAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCg                                                                                                                                <  2:1010486/150‑1 (MQ=255)

                      tgATAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGt                                                                                                                               <  1:1443313/150‑1 (MQ=255)

                       gAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTg                                                                                                                              >  2:528262/1‑150 (MQ=255)

                          aGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAg                                                                                                                           >  1:497726/1‑150 (MQ=255)

                           ggCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGt                                                                                                                          >  1:690951/1‑150 (MQ=255)

                            gCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                                                                         >  2:1340905/1‑150 (MQ=255)

                            gCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                                                                         >  1:216036/1‑150 (MQ=255)

                            gCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCAAAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGAACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                                                                         >  2:6272/1‑150 (MQ=255)

                                  tttATCGTTAGTCAGCCTGCGTTGTGTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAAtt                                                                                                                   <  2:1216826/150‑1 (MQ=255)

                                    tATCGTTTGTCAGCATGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTcc                                                                                                                 <  2:1394512/150‑1 (MQ=255)

                                      tCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                                                                               <  2:143386/150‑1 (MQ=255)

                                      tCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                                                                               >  1:1379958/1‑150 (MQ=255)

                                      tCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                                                                               <  1:1338792/150‑1 (MQ=255)

                                           tGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTTATCACAGGGGAAGGTGAGATATGGACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTTTCAGCATCGTGTTAATTGTTCTGACCATCGTGAAGTTCTAATTCCACCCGtt                                                                                                          >  1:860014/1‑150 (MQ=255)

                                             tCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATCTGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACTATCGTGAAGTTCAAATTCCACCCGTTTc                                                                                                        <  1:771769/150‑1 (MQ=255)

                                             tCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTc                                                                                                        >  1:1706639/1‑150 (MQ=255)

                                                gCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGATGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTgg                                                                                                     >  1:921517/1‑150 (MQ=255)

                                                  cTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGgcg                                                                                                   <  1:998461/150‑1 (MQ=255)

                                                       ttgttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACAATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGtt                                                                                              >  2:883928/1‑150 (MQ=255)

                                                         gttTTTTTGTCGAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGc                                                                                            <  2:1488201/150‑1 (MQ=255)

                                                             ttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGc                                                                                        <  1:920566/150‑1 (MQ=255)

                                                              tttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGcc                                                                                       <  2:1601213/150‑1 (MQ=255)

                                                                 gTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGc                                                                                    <  2:1628099/150‑1 (MQ=255)

                                                                  tCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGct                                                                                   <  2:235291/150‑1 (MQ=255)

                                                                  tCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGct                                                                                   <  2:439451/150‑1 (MQ=255)

                                                                   ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                                                                                  >  2:415895/1‑150 (MQ=255)

                                                                   ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                                                                                  >  2:632692/1‑150 (MQ=255)

                                                                   ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                                                                                  >  1:787259/1‑150 (MQ=255)

                                                                    cAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGcttc                                                                                 <  2:161900/150‑1 (MQ=255)

                                                                        atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                             <  2:473012/150‑1 (MQ=255)

                                                                        atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                             <  2:645753/150‑1 (MQ=255)

                                                                        atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                             <  2:954364/150‑1 (MQ=255)

                                                                        atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCg                                                                             <  1:1541926/150‑1 (MQ=255)

                                                                          tatcaGGTTTATCACTGGGGAAGGTGAGATTTGCACTCTCAAATCT‑GGGTTGTGAGCACTCTGCTTATCAGCATCGTGTTAATTGTACTGAACATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTg                                                                           <  2:156194/149‑1 (MQ=255)

                                                                           atcaGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTgg                                                                          >  2:11300/1‑150 (MQ=255)

                                                                             caGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCCTCGTGGGa                                                                        >  2:743436/1‑150 (MQ=255)

                                                                              aGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCGGGCCAGCTTCTTCGTGGGaa                                                                       <  1:311524/150‑1 (MQ=255)

                                                                                  tAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgat                                                                   >  1:442614/1‑150 (MQ=255)

                                                                                   aaTCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGCACgatg                                                                  >  2:855624/1‑150 (MQ=255)

                                                                                         aGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGc                                                            >  2:1028165/1‑150 (MQ=255)

                                                                                          ggggAAGGTGAGATATGCACTCTCAAATCT‑GGGGTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCa                                                           <  2:1531815/150‑1 (MQ=255)

                                                                                              aaGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTTCTGACCATCGTGAAGTTCTAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATggg                                                       <  1:1150512/150‑1 (MQ=255)

                                                                                               aGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATgggg                                                      <  2:527912/150‑1 (MQ=255)

                                                                                               aGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATgggg                                                      <  1:1245236/150‑1 (MQ=255)

                                                                                                 gTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGcc                                                    >  1:552432/1‑150 (MQ=255)

                                                                                                  tGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCa                                                   >  1:853731/1‑150 (MQ=255)

                                                                                                      atatGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTgg                                               >  2:1704703/1‑150 (MQ=255)

                                                                                                      atatGCACTCTCAAATCT‑GGGTGGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTgg                                               <  2:1323768/150‑1 (MQ=255)

                                                                                                        atGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGat                                             >  2:785193/1‑150 (MQ=255)

                                                                                                        atGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGat                                             >  2:1527719/1‑150 (MQ=255)

                                                                                                         tGCACTCTCAAATCTGGGGTTGTGAGCCCGCTGCTTATCAGCATCGTGTTAATTGTACTGACCAGCGTGAAGTGCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGat                                             <  2:1176610/150‑1 (MQ=255)

                                                                                                          gCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGATGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGatat                                           >  1:250983/1‑150 (MQ=255)

                                                                                                           cACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATg                                          >  1:1180164/1‑150 (MQ=255)

                                                                                                            aCTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATgg                                         <  1:1068572/150‑1 (MQ=255)

                                                                                                             ctctCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGt                                        >  2:1054565/1‑150 (MQ=255)

                                                                                                             ctctCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGATTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGt                                        >  1:692766/1‑150 (MQ=255)

                                                                                                                   aactcggggTTGTGAGCCCGCAGCTTATCAGCATCGTGTTAATTCTACTGACCCTCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCGGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATg                                   <  1:854573/144‑1 (MQ=255)

                                                                                                                      cT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGATGCTGGCCAGATTCTTAGTGGGAACGATGATTGGCATGGGGACACTGGATATGGTAAATGCTAt                               >  2:1045719/1‑150 (MQ=255)

                                                                                                                         gggTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTg                             >  2:1198558/1‑150 (MQ=255)

                                                                                                                          ggTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGa                            >  1:1292593/1‑150 (MQ=255)

                                                                                                                           gTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCAAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGaa                           >  1:1070766/1‑150 (MQ=255)

                                                                                                                             tgtgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAg                         <  1:528262/150‑1 (MQ=255)

                                                                                                                             aattaacACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAg                         >  2:408803/7‑150 (MQ=255)

                                                                                                                              gtgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGt                        <  1:1340905/150‑1 (MQ=255)

                                                                                                                               ttaacACGATGCTGATAAGCAGCGTGGTAATTGTACTGACCATCGTGAAGTTCAAATTCCACACGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGAGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg                       >  1:899378/5‑150 (MQ=255)

                                                                                                                               tgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg                       >  1:1462973/1‑150 (MQ=255)

                                                                                                                                 cgCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACGATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGTTAAATGCTATTGAAAGTTGa                     <  1:6272/149‑1 (MQ=255)

                                                                                                                                   cACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATAAGGTAAATGCTATTGAAAGTGGAAt                   >  1:932018/1‑150 (MQ=255)

                                                                                                                                   cACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg                       <  1:1379290/146‑1 (MQ=255)

                                                                                                                                   cACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg                       >  2:1379290/1‑146 (MQ=255)

                                                                                                                                   cACGATGCTGATAAGCAGCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAAt                   >  1:320982/1‑150 (MQ=255)

                                                                                                                                     cGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTg                 >  1:164607/1‑150 (MQ=255)

                                                                                                                                      gctgctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTgg                <  1:1011993/150‑1 (MQ=255)

                                                                                                                                       ctgctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGc               <  2:818741/150‑1 (MQ=255)

                                                                                                                                       ctgctTATCAGCATCGTGTTAATGGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTCCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGc               <  2:818738/150‑1 (MQ=255)

                                                                                                                                         gctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCgg             <  2:1243658/150‑1 (MQ=255)

                                                                                                                                         gctTATCAGCATCGGGGTCATTGTACTGACCATCGTGAAGTTCACATGCCACCCGTTTCTGGCGCTGGTGCTGGCCAGCTTCTTCGTGGGAACGATGAGGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCgg             <  2:830339/150‑1 (MQ=255)

                                                                                                                                            tATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAAc          >  1:1187844/1‑150 (MQ=255)

                                                                                                                                            tATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAAc          >  1:1530242/1‑150 (MQ=255)

                                                                                                                                            tATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCAGGCTCTTTTGCTGGCAAGCTTCTTCGTGGTAACGATGATGTGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAAc          >  1:1070805/1‑150 (MQ=255)

                                                                                                                                                aGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTg      <  1:472067/150‑1 (MQ=255)

                                                                                                                                                aGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTg      >  2:1214444/1‑150 (MQ=255)

                                                                                                                                                aGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTg      >  1:1309130/1‑150 (MQ=255)

                                                                                                                                                 gCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTgg     <  1:311147/150‑1 (MQ=255)

                                                                                                                                                 gCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTgg     <  1:632692/150‑1 (MQ=255)

                                                                                                                                                 gCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGAGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTgg     <  2:216036/150‑1 (MQ=255)

                                                                                                                                                  cATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTggg    <  2:788678/150‑1 (MQ=255)

CATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCT‑GGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGT  >  NC_000913/2477743‑2478039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`