BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	297,212	C→A	5.3%	pseudogene (7/49 nt)	ptwF →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	297,212	0	C	A	5.3%	384.8 / 5.7	129	pseudogene (7/49 nt)	ptwF	tRNA‑OTHER
Reads supporting (aligned to +/- strand): ref base C (58/63); new base A (5/3); total (63/66)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CTGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACA  >  NC_000913/297064‑297357

ctgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCg                                                                                                                                                  <  2:381233/150‑1 (MQ=255)

 tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGt                                                                                                                                                 <  1:743570/150‑1 (MQ=255)

 tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGt                                                                                                                                                 <  1:1226135/150‑1 (MQ=255)

     ggTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATg                                                                                                                                             >  2:1578908/1‑150 (MQ=255)

      gtgtAAGTATATGGAGGGAATTAACATCAACTTTTGTTAATTTCAATCTACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGTATTAACATCGCATTCGTAATga                                                                                                                                            >  2:715078/1‑149 (MQ=255)

       tgtAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCg                                                                                                                                           <  1:223509/150‑1 (MQ=255)

       tgtAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCg                                                                                                                                           <  1:1470204/150‑1 (MQ=255)

         taagtaaATGTAGTGAATAAACATAAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATTACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGATCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGat                                                                                                                                         <  1:1027454/150‑2 (MQ=255)

          aagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGAATTAACATCGCATTCGTAATGCGAAg                                                                                                                                        >  1:344239/1‑150 (MQ=255)

           agtaaATGGAGGGAATAAACATCATCTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGTTTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                       <  2:1645619/150‑1 (MQ=255)

           agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGTTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                       >  2:35900/1‑150 (MQ=255)

           agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                       >  1:1348361/1‑150 (MQ=255)

           agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                       >  2:1276431/1‑150 (MQ=255)

            gtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGatgtt                                                                                                                                      <  2:489227/150‑5 (MQ=255)

               aaTGGAGGGAATAAACATCAAGTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGt                                                                                                                                   <  1:1429726/150‑1 (MQ=255)

               aaTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGTTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGt                                                                                                                                   <  1:117786/150‑1 (MQ=255)

               aaTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGt                                                                                                                                   <  1:1304861/150‑1 (MQ=255)

               aaTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGatgttaat                                                                                                                                   <  1:392824/150‑8 (MQ=255)

                aTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTa                                                                                                                                  <  1:798073/150‑1 (MQ=255)

                    aGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGTGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                              <  2:1575126/150‑1 (MQ=255)

                     gggAATAAACATAAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATTACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGAATTAGTAATGCGAAGGTCTTAGGTTc                                                                                                                             >  2:463447/1‑150 (MQ=255)

                      ggAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCg                                                                                                                            <  1:469057/150‑1 (MQ=255)

                      ggAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCg                                                                                                                            <  1:609745/150‑1 (MQ=255)

                      ggAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTT‑AAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTcta                                                                                                                           >  2:1044468/1‑148 (MQ=255)

                        aaTAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGaa                                                                                                                          >  1:131193/1‑149 (MQ=255)

                        aaTAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGAc                                                                                                                          <  1:353630/150‑1 (MQ=255)

                         aTAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACt                                                                                                                         <  1:588336/150‑1 (MQ=255)

                          tAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTc                                                                                                                        <  1:1638247/150‑1 (MQ=255)

                          tAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTc                                                                                                                        >  2:107461/1‑150 (MQ=255)

                           aaaCATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTcc                                                                                                                       <  2:927071/150‑1 (MQ=255)

                           aaaCATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTcc                                                                                                                       <  2:981374/150‑1 (MQ=255)

                              cATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtat                                                                                                                    >  2:1295230/1‑150 (MQ=255)

                                tCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGAATCCtatta                                                                                                                  >  1:355252/1‑150 (MQ=255)

                                 cAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAAAATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtattat                                                                                                                 >  2:1280021/1‑150 (MQ=255)

                                    cTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCgg                                                                                                              >  2:698932/1‑150 (MQ=255)

                                         gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                         >  2:611913/1‑150 (MQ=255)

                                             aTTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaa                                                                                                     >  1:1474728/1‑150 (MQ=255)

                                             aTTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaa                                                                                                     >  1:1574437/1‑150 (MQ=255)

                                               ttCAATCAACGGGTTTATTGCTATAACGTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaaaa                                                                                                   <  1:1311867/150‑1 (MQ=255)

                                                tcaatcaaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCTCATTAGTAATGCGAAGGTCGTAGGTTCGACACCTATTATCGGCACCATTaaaaa                                                                                                  >  1:696459/1‑149 (MQ=255)

                                                       aCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCTGCACCATTAAAATCAAagag                                                                                           >  1:105641/1‑150 (MQ=255)

                                                       aCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAagag                                                                                           >  1:1401403/1‑150 (MQ=255)

                                                        cGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCTAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGt                                                                                          >  1:896569/1‑150 (MQ=255)

                                                         gggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGAATTCGTAATGCGAAGGTAGTAGGTTCGACTCATATTATCGGCACCATTAAAATCAAAGAGtt                                                                                         >  1:822258/1‑150 (MQ=255)

                                                         gggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGGTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGAATTAACATCTAATTCGTATTGAGAAGGTCTTAGGTTCGACTCCTATTATCGTAACAATTATAATAAAAGAGtt                                                                                         >  1:330701/1‑150 (MQ=255)

                                                             ttATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                     >  1:640609/1‑150 (MQ=255)

                                                              tATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAcccc                                                                                    <  2:476898/150‑1 (MQ=255)

                                                                ttGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCat                                                                                  <  2:1051963/150‑1 (MQ=255)

                                                                   cTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATAtt                                                                               >  2:1654983/1‑150 (MQ=255)

                                                                     ataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAATGAGTTACCCCATATTTa                                                                             >  2:10408/1‑150 (MQ=255)

                                                                     ataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTa                                                                             >  2:566639/1‑150 (MQ=255)

                                                                        aCTTGGTGATTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATAATCGGCACCATTAACATCAAAGAGTTACCCCATATTTAAAt                                                                          <  2:1269078/150‑1 (MQ=255)

                                                                          ttGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATac                                                                        <  1:1177653/150‑1 (MQ=255)

                                                                          ttGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATac                                                                        <  1:1664853/150‑1 (MQ=255)

                                                                           tGGTGTTTTATAATATAAAGTATGTTGTTTTGATAGATAGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAAAGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATaca                                                                       >  2:967012/1‑150 (MQ=255)

                                                                            ggagtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                      <  1:241838/147‑1 (MQ=255)

                                                                            ggTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                      >  2:1156661/1‑150 (MQ=255)

                                                                            ggTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                      <  1:1118346/150‑1 (MQ=255)

                                                                             gtgtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcacc                                                                     >  1:1034533/1‑150 (MQ=255)

                                                                              tgtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcacca                                                                    >  1:1339448/1‑150 (MQ=255)

                                                                              tgtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcacca                                                                    <  1:877597/150‑1 (MQ=255)

                                                                                   tataatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGttt                                                               <  2:1401403/150‑1 (MQ=255)

                                                                                     taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                             >  1:922953/1‑150 (MQ=255)

                                                                                     taatataaAGTATGATGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                             <  1:947639/150‑1 (MQ=255)

                                                                                      aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcct                                                            >  2:1376194/1‑150 (MQ=255)

                                                                                      aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGGTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcct                                                            <  2:1223002/150‑1 (MQ=255)

                                                                                       atataaAGTATGTTGGTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcctc                                                           <  1:941142/150‑1 (MQ=255)

                                                                                           aaaGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTCCCCCATATTTAAATACACCACGTTTCCTCCtgt                                                       >  1:1089213/1‑150 (MQ=255)

                                                                                                 tgttgtTTTGATTGATTGCTCAAGTAGTTAAAATTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTa                                                 <  2:350701/150‑1 (MQ=255)

                                                                                                  gttgttTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTAt                                                >  2:1583488/1‑150 (MQ=255)

                                                                                                   ttgttTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTAtt                                               <  1:482981/150‑1 (MQ=255)

                                                                                                    tgttTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTTAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTAttt                                              <  1:668283/150‑1 (MQ=255)

                                                                                                      ttttGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTtgt                                            <  2:814808/150‑1 (MQ=255)

                                                                                                          gattgattGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCa                                        >  2:79706/1‑150 (MQ=255)

                                                                                                           attgattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCAt                                       <  1:1024897/150‑1 (MQ=255)

                                                                                                             tgattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTg                                     <  1:1578908/150‑1 (MQ=255)

                                                                                                                   cTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCt                               <  1:474926/150‑1 (MQ=255)

                                                                                                                   cTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCt                               >  1:985797/1‑150 (MQ=255)

                                                                                                                       agtagtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGc                           <  1:1698322/150‑1 (MQ=255)

                                                                                                                          agtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAATTACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCGTGGCAAt                        >  2:873900/1‑150 (MQ=255)

                                                                                                                          agtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAAt                        >  1:274168/1‑150 (MQ=255)

                                                                                                                             tAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAACCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtca                     >  1:1543386/1‑150 (MQ=255)

                                                                                                                               aaaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatc                   <  2:186245/150‑1 (MQ=255)

                                                                                                                                aaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatca                  <  1:1228140/150‑1 (MQ=255)

                                                                                                                                aaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatca                  <  2:537412/150‑1 (MQ=255)

                                                                                                                                 aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                 >  1:552698/1‑150 (MQ=255)

                                                                                                                                    gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt              >  1:680710/1‑150 (MQ=255)

                                                                                                                                    gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt              <  1:222467/150‑1 (MQ=255)

                                                                                                                                    gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt              <  1:42366/150‑1 (MQ=255)

                                                                                                                                    gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt              <  2:1339448/150‑1 (MQ=255)

                                                                                                                                     cATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATa             >  2:532329/1‑150 (MQ=255)

                                                                                                                                     cATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATa             >  2:1713612/1‑150 (MQ=255)

                                                                                                                                         aaCATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt         <  1:79706/150‑1 (MQ=255)

                                                                                                                                          ccttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTAATATCGGCACCAAAAAAATCAAAGAGTTACCCCATATTTAAATACACCACGATTCCTCCAGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGtt        >  2:499136/4‑150 (MQ=38)

                                                                                                                                           cttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTc       >  2:638684/3‑150 (MQ=255)

                                                                                                                                            aTCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATAAAAGAGTTACCCCATATTTAAATACAACACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATCCTGTTCt      <  1:231951/150‑1 (MQ=255)

                                                                                                                                             tCGCATTCGTAATGCGAATGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTAAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACATTGGCAATTCATCAAAATACTGTTCTg     <  1:463447/150‑1 (MQ=255)

                                                                                                                                             tCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTg     >  1:422762/1‑150 (MQ=255)

                                                                                                                                              cGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCAAATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAGTGTTCTGa    >  2:1135472/1‑150 (MQ=255)

                                                                                                                                               gCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGAc   >  1:1128328/1‑150 (MQ=255)

CTGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACA  >  NC_000913/297064‑297357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`