Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,511,424 T→A 6.5% intergenic (+97/+44) ypeC → / ← mntH DUF2502 family putative periplasmic protein/manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,4240TA 6.5% 256.5 / 5.1 89intergenic (+97/+44)ypeC/mntHDUF2502 family putative periplasmic protein/manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base T (40/43);  new base A (3/3);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAG  >  NC_000913/2511275‑2511569
                                                                                                                                                     |                                                                                                                                                 
gcgATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGtt                                                                                                                                                   >  2:881235/1‑150 (MQ=255)
gcgAGCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGtt                                                                                                                                                   <  1:1244272/150‑1 (MQ=255)
   aTCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTACCGCGTAGGGCGGATAAGGCGTTTAc                                                                                                                                                >  2:1338432/1‑150 (MQ=255)
   aTCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTAc                                                                                                                                                <  1:851769/150‑1 (MQ=255)
   aTCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTAc                                                                                                                                                <  1:913921/150‑1 (MQ=255)
    tCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATCGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTAcg                                                                                                                                               <  1:1383010/150‑1 (MQ=255)
      gTGACTATCCTCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCTTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTAcgcc                                                                                                                                             <  1:1477190/150‑1 (MQ=255)
      gTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTAcgcc                                                                                                                                             <  2:17546/150‑1 (MQ=255)
        gACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTATTATCAGGCCTACAAACCGGCTCTTTAACGCCTAGGGCGGATAAGGCGTTTAcgacgc                                                                                                                                           >  1:1516981/1‑150 (MQ=255)
            atcatcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                       <  2:23721/150‑1 (MQ=39)
            atcatcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                       <  1:1311020/150‑1 (MQ=255)
              catcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCCTCCGc                                                                                                                                     >  2:1525276/1‑150 (MQ=255)
              catcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGc                                                                                                                                     <  2:80715/150‑1 (MQ=255)
              aatcGCGGAAATTGCCGGGGTCATTTGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGAGGCTAGTTCGTGTTATCAGGCCTACAAACCGGCTCTTTAACGCGTATTGCTGATAATGCGTTTACGCCGCATCCGc                                                                                                                                     <  2:1282914/149‑1 (MQ=255)
               atcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGCCGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGcc                                                                                                                                    >  1:296042/1‑150 (MQ=255)
                  gcgGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAAt                                                                                                                                 <  2:1238431/150‑1 (MQ=255)
                    ggAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAg                                                                                                                               >  1:673493/1‑150 (MQ=255)
                      aaaaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTg                                                                                                                             <  2:95477/150‑1 (MQ=255)
                      aaaaGGCCGGGGTCATGGGCACCGCCAGTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGTCGTATGCGGCGTAAACGCCTTATCCGccctacgcg                                                                                                                             <  1:1379485/150‑8 (MQ=37)
                       aaaGGCCGGGGTCCTGGGCACCGCCATTACACGCGTCAGCCAATGGAGCAGAATGCCTGATGCGACGCTAGAGCGACTTATCAGGCCTACAAACAGTCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACTCCGCATCCGCCAATAGTGc                                                                                                                            <  1:292421/150‑1 (MQ=37)
                        aaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGcc                                                                                                                           >  1:81566/1‑150 (MQ=255)
                        aaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGcc                                                                                                                           >  1:1204541/1‑150 (MQ=255)
                             cGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATg                                                                                                                      >  1:69799/1‑150 (MQ=255)
                               gggTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCg                                                                                                                    >  2:1511986/1‑150 (MQ=255)
                                 gTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGAc                                                                                                                  <  1:1627716/150‑1 (MQ=255)
                                    aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCt                                                                                                               <  2:1637828/150‑1 (MQ=255)
                                    aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCt                                                                                                               <  2:1214603/150‑1 (MQ=255)
                                       ggCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCAt                                                                                                            >  1:491558/1‑150 (MQ=255)
                                         cACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTc                                                                                                          <  2:1103032/150‑1 (MQ=255)
                                            cGCCATAAAACGCGGCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTATACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAAc                                                                                                       <  1:307558/150‑1 (MQ=255)
                                             gCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACt                                                                                                      <  1:323345/150‑1 (MQ=255)
                                             gCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACt                                                                                                      >  1:491942/1‑150 (MQ=255)
                                             gCCATTAAACGCGTCCGCCAAAGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTATGGCGGATAAGGCGTTTAAGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACt                                                                                                      <  2:915043/150‑1 (MQ=255)
                                                   aaaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATc                                                                                                >  1:57647/1‑150 (MQ=255)
                                                    aaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATcc                                                                                               <  1:1484594/150‑1 (MQ=255)
                                                          tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAgcgc                                                                                         <  2:151278/150‑1 (MQ=255)
                                                            cGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACCATCCCAGCGCCg                                                                                       >  1:1487440/1‑150 (MQ=255)
                                                             gCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCACAGCGCCGt                                                                                      >  2:530253/1‑150 (MQ=255)
                                                                  tGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                 <  2:330965/150‑1 (MQ=255)
                                                                  tGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                 <  1:1336472/150‑1 (MQ=255)
                                                                    gAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCccacc                                                                               <  2:369124/150‑1 (MQ=255)
                                                                    gAGCACAATACATGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCccacc                                                                               >  2:636135/1‑150 (MQ=255)
                                                                     aGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCccacca                                                                              >  2:136261/1‑150 (MQ=255)
                                                                       cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCACAGCGCCGTCCCCACcaac                                                                            >  2:979120/1‑150 (MQ=255)
                                                                        acaATGCCTGATGCGACGCTAGTGCGTCTTATCATGCCTACAAACCGTCTCTTTAACGCGTAGGGCGTATAAGGCGTTTACGCCGCATCCGACAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaaca                                                                           <  2:1190676/150‑1 (MQ=255)
                                                                         caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaacaa                                                                          >  2:544266/1‑150 (MQ=255)
                                                                         caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaacaa                                                                          >  2:1714316/1‑150 (MQ=255)
                                                                           aTGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAAcc                                                                        <  2:602926/150‑1 (MQ=255)
                                                                             gCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAg                                                                      <  1:1443346/150‑1 (MQ=255)
                                                                             gCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGAGGATAAGGCGATTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACACTCCCAGCGCCGTCCCCACCAACAACCAg                                                                      >  1:535427/1‑150 (MQ=255)
                                                                              ccTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGa                                                                     <  1:1245313/150‑1 (MQ=255)
                                                                                tGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata                                                                   >  1:1423476/1‑150 (MQ=255)
                                                                                          cTAGTGCGTCTTATCAGTCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCTTTTACGCCGCATACGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCa                                                         <  1:1402960/150‑1 (MQ=255)
                                                                                            aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                       <  2:1204541/150‑1 (MQ=255)
                                                                                            aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                       >  2:1264198/1‑150 (MQ=255)
                                                                                            aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                       <  2:81566/150‑1 (MQ=255)
                                                                                                 gTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGCTATTCAGCGCCACGACCAg                                                  >  1:3319/1‑150 (MQ=255)
                                                                                                 gTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGTGCCACGACCAg                                                  <  1:881235/150‑1 (MQ=255)
                                                                                                  tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                 >  1:1622750/1‑150 (MQ=255)
                                                                                                  tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                 >  2:298440/1‑150 (MQ=255)
                                                                                                   cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGCCCAGcc                                                >  2:1447416/1‑149 (MQ=255)
                                                                                                   cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                >  2:1454156/1‑150 (MQ=255)
                                                                                                   cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                <  2:1577863/150‑1 (MQ=255)
                                                                                                     tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                                              <  2:1355962/150‑1 (MQ=255)
                                                                                                     tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAATCAGCGCCACGACCAGcacc                                              <  1:258931/150‑1 (MQ=255)
                                                                                                        cAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGACGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                           >  2:1627312/1‑150 (MQ=255)
                                                                                                         aGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACaa                                          <  2:528317/150‑1 (MQ=255)
                                                                                                           gCCTACAAACCGGCTCTTTAACTCTTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATGCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATc                                        <  2:535427/150‑1 (MQ=255)
                                                                                                           gCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATc                                        >  1:583625/1‑150 (MQ=255)
                                                                                                             cTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCACCAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc                                      >  2:1495704/1‑150 (MQ=255)
                                                                                                              tACAAACCGGCTCTTTAACGCGTAGGGCTGATAAGGCGTTTACGCCGCCTCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGGTATTAAGCGCCCCGCCCCGCACAACAATCAcc                                     >  1:53586/1‑150 (MQ=255)
                                                                                                                        ctctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtct                           <  2:583082/150‑1 (MQ=255)
                                                                                                                        ctctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACAATAGTGCCAGATGCGACGCACATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgacg                           >  1:1382324/1‑147 (MQ=255)
                                                                                                                         tctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc                                      <  1:1220472/138‑1 (MQ=255)
                                                                                                                         tctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc                                      >  2:1220472/1‑138 (MQ=255)
                                                                                                                            ttAACGAGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGAAGCTCATTCAACTACAATCCCAGCGCAGTCCCCACCAACATCCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGttt                       <  1:204585/150‑1 (MQ=255)
                                                                                                                              aaCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCCCCACCATCACCCAGCCTGTCTGTTTTa                     >  1:63375/1‑150 (MQ=255)
                                                                                                                              aaCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACAACAATCACGCAGCCTGTCGGTTTTa                     >  2:981888/1‑150 (MQ=255)
                                                                                                                                    attggCGGATGCGGCGTAAACGCCTTATCCGCCAATAGTGCCAGATGCGCCGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTGTTACGCGtt               >  2:929504/4‑150 (MQ=255)
                                                                                                                                     ttggCGGATGCGGCGTAAACGCCTTATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACACCCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGttt              >  1:685056/3‑150 (MQ=255)
                                                                                                                                     aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGttt              >  2:165128/1‑150 (MQ=255)
                                                                                                                                     aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGAAGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCACCAACCAGATATTCAGCGCCACGACCCGCACCACCATCACCCAGCCTGTCTGATTTACGCGttt              >  1:203307/1‑150 (MQ=39)
                                                                                                                                       ggCGGATGCGGCGTAAACGCCTTATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGc            >  1:1464596/1‑150 (MQ=255)
                                                                                                                                       ggCGGATAAGGCGTTTACGGCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGc            <  2:1516981/150‑1 (MQ=255)
                                                                                                                                        gCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACCATCCCCCGGCCTGTCTGTTTTACGCGTTTGCt           >  1:1511130/1‑150 (MQ=255)
                                                                                                                                         cgtaTAAGGCGTTTACGCAGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTg          <  2:1382324/147‑1 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCAcc    <  2:491558/150‑1 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCACACCAACAACCAGATATTCAGCTCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCAcc    >  2:33092/1‑150 (MQ=255)
                                                                                                                                                ggCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAAGCAGATATTCAGCGCCACGCCCAGCCCCCCAATCCCCCAGCCTGGCTGGTTTACGCGTGTGCTGtaccccc   >  1:344987/1‑144 (MQ=255)
                                                                                                                                                 gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAg  <  1:1597713/150‑1 (MQ=255)
                                                                                                                                                 gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAg  <  1:136261/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAG  >  NC_000913/2511275‑2511569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: