Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,511,432 C→T 6.0% intergenic (+105/+36) ypeC → / ← mntH DUF2502 family putative periplasmic protein/manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,4320CT 6.0% 251.5 / 4.0 83intergenic (+105/+36)ypeC/mntHDUF2502 family putative periplasmic protein/manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base C (38/40);  new base T (3/2);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

GACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCA  >  NC_000913/2511283‑2511580
                                                                                                                                                     |                                                                                                                                                    
gACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTATTATCAGGCCTACAAACCGGCTCTTTAACGCCTAGGGCGGATAAGGCGTTTAcgacgc                                                                                                                                                      >  1:1516981/1‑150 (MQ=255)
    atcatcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                  <  2:23721/150‑1 (MQ=39)
    atcatcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                                  <  1:1311020/150‑1 (MQ=255)
      catcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCCTCCGc                                                                                                                                                >  2:1525276/1‑150 (MQ=255)
      catcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGc                                                                                                                                                <  2:80715/150‑1 (MQ=255)
      aatcGCGGAAATTGCCGGGGTCATTTGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGAGGCTAGTTCGTGTTATCAGGCCTACAAACCGGCTCTTTAACGCGTATTGCTGATAATGCGTTTACGCCGCATCCGc                                                                                                                                                <  2:1282914/149‑1 (MQ=255)
       atcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGCCGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGcc                                                                                                                                               >  1:296042/1‑150 (MQ=255)
          gcgGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAAt                                                                                                                                            <  2:1238431/150‑1 (MQ=255)
            ggAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAg                                                                                                                                          >  1:673493/1‑150 (MQ=255)
              aaaaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTg                                                                                                                                        <  2:95477/150‑1 (MQ=255)
              aaaaGGCCGGGGTCATGGGCACCGCCAGTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGTCGTATGCGGCGTAAACGCCTTATCCGccctacgcg                                                                                                                                        <  1:1379485/150‑8 (MQ=37)
               aaaGGCCGGGGTCCTGGGCACCGCCATTACACGCGTCAGCCAATGGAGCAGAATGCCTGATGCGACGCTAGAGCGACTTATCAGGCCTACAAACAGTCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACTCCGCATCCGCCAATAGTGc                                                                                                                                       <  1:292421/150‑1 (MQ=37)
                aaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGcc                                                                                                                                      >  1:81566/1‑150 (MQ=255)
                aaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGcc                                                                                                                                      >  1:1204541/1‑150 (MQ=255)
                     cGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATg                                                                                                                                 >  1:69799/1‑150 (MQ=255)
                       gggTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCg                                                                                                                               >  2:1511986/1‑150 (MQ=255)
                         gTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGAc                                                                                                                             <  1:1627716/150‑1 (MQ=255)
                            aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCt                                                                                                                          <  2:1637828/150‑1 (MQ=255)
                            aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCt                                                                                                                          <  2:1214603/150‑1 (MQ=255)
                               ggCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCAt                                                                                                                       >  1:491558/1‑150 (MQ=255)
                                 cACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTc                                                                                                                     <  2:1103032/150‑1 (MQ=255)
                                    cGCCATAAAACGCGGCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTATACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAAc                                                                                                                  <  1:307558/150‑1 (MQ=255)
                                     gCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACt                                                                                                                 >  1:491942/1‑150 (MQ=255)
                                     gCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACt                                                                                                                 <  1:323345/150‑1 (MQ=255)
                                     gCCATTAAACGCGTCCGCCAAAGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTATGGCGGATAAGGCGTTTAAGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACt                                                                                                                 <  2:915043/150‑1 (MQ=255)
                                           aaaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATc                                                                                                           >  1:57647/1‑150 (MQ=255)
                                            aaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATcc                                                                                                          <  1:1484594/150‑1 (MQ=255)
                                                  tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAgcgc                                                                                                    <  2:151278/150‑1 (MQ=255)
                                                    cGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACCATCCCAGCGCCg                                                                                                  >  1:1487440/1‑150 (MQ=255)
                                                     gCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCACAGCGCCGt                                                                                                 >  2:530253/1‑150 (MQ=255)
                                                          tGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                            <  1:1336472/150‑1 (MQ=255)
                                                          tGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCcca                                                                                            <  2:330965/150‑1 (MQ=255)
                                                            gAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCccacc                                                                                          <  2:369124/150‑1 (MQ=255)
                                                            gAGCACAATACATGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCccacc                                                                                          >  2:636135/1‑150 (MQ=255)
                                                             aGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCccacca                                                                                         >  2:136261/1‑150 (MQ=255)
                                                               cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCACAGCGCCGTCCCCACcaac                                                                                       >  2:979120/1‑150 (MQ=255)
                                                                acaATGCCTGATGCGACGCTAGTGCGTCTTATCATGCCTACAAACCGTCTCTTTAACGCGTAGGGCGTATAAGGCGTTTACGCCGCATCCGACAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaaca                                                                                      <  2:1190676/150‑1 (MQ=255)
                                                                 caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaacaa                                                                                     >  2:1714316/1‑150 (MQ=255)
                                                                 caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaacaa                                                                                     >  2:544266/1‑150 (MQ=255)
                                                                   aTGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAAcc                                                                                   <  2:602926/150‑1 (MQ=255)
                                                                     gCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAg                                                                                 <  1:1443346/150‑1 (MQ=255)
                                                                     gCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGAGGATAAGGCGATTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACACTCCCAGCGCCGTCCCCACCAACAACCAg                                                                                 >  1:535427/1‑150 (MQ=255)
                                                                      ccTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGa                                                                                <  1:1245313/150‑1 (MQ=255)
                                                                        tGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata                                                                              >  1:1423476/1‑150 (MQ=255)
                                                                                  cTAGTGCGTCTTATCAGTCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCTTTTACGCCGCATACGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCa                                                                    <  1:1402960/150‑1 (MQ=255)
                                                                                    aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                  <  2:1204541/150‑1 (MQ=255)
                                                                                    aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                  >  2:1264198/1‑150 (MQ=255)
                                                                                    aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                  <  2:81566/150‑1 (MQ=255)
                                                                                         gTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGCTATTCAGCGCCACGACCAg                                                             >  1:3319/1‑150 (MQ=255)
                                                                                         gTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGTGCCACGACCAg                                                             <  1:881235/150‑1 (MQ=255)
                                                                                          tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                            >  1:1622750/1‑150 (MQ=255)
                                                                                          tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                            >  2:298440/1‑150 (MQ=255)
                                                                                           cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGCCCAGcc                                                           >  2:1447416/1‑149 (MQ=255)
                                                                                           cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                           <  2:1577863/150‑1 (MQ=255)
                                                                                           cTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                           >  2:1454156/1‑150 (MQ=255)
                                                                                             tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                                                         <  2:1355962/150‑1 (MQ=255)
                                                                                             tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAATCAGCGCCACGACCAGcacc                                                         <  1:258931/150‑1 (MQ=255)
                                                                                                cAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGACGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                      >  2:1627312/1‑150 (MQ=255)
                                                                                                 aGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACaa                                                     <  2:528317/150‑1 (MQ=255)
                                                                                                   gCCTACAAACCGGCTCTTTAACTCTTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATGCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATc                                                   <  2:535427/150‑1 (MQ=255)
                                                                                                   gCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATc                                                   >  1:583625/1‑150 (MQ=255)
                                                                                                     cTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCACCAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc                                                 >  2:1495704/1‑150 (MQ=255)
                                                                                                      tACAAACCGGCTCTTTAACGCGTAGGGCTGATAAGGCGTTTACGCCGCCTCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGGTATTAAGCGCCCCGCCCCGCACAACAATCAcc                                                >  1:53586/1‑150 (MQ=255)
                                                                                                                ctctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtct                                      <  2:583082/150‑1 (MQ=255)
                                                                                                                ctctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACAATAGTGCCAGATGCGACGCACATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgacg                                      >  1:1382324/1‑147 (MQ=255)
                                                                                                                 tctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc                                                 <  1:1220472/138‑1 (MQ=255)
                                                                                                                 tctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc                                                 >  2:1220472/1‑138 (MQ=255)
                                                                                                                    ttAACGAGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGAAGCTCATTCAACTACAATCCCAGCGCAGTCCCCACCAACATCCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGttt                                  <  1:204585/150‑1 (MQ=255)
                                                                                                                      aaCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCCCCACCATCACCCAGCCTGTCTGTTTTa                                >  1:63375/1‑150 (MQ=255)
                                                                                                                      aaCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACAACAATCACGCAGCCTGTCGGTTTTa                                >  2:981888/1‑150 (MQ=255)
                                                                                                                            attggCGGATGCGGCGTAAACGCCTTATCCGCCAATAGTGCCAGATGCGCCGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTGTTACGCGtt                          >  2:929504/4‑150 (MQ=255)
                                                                                                                             ttggCGGATGCGGCGTAAACGCCTTATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACACCCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGttt                         >  1:685056/3‑150 (MQ=255)
                                                                                                                             aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGttt                         >  2:165128/1‑150 (MQ=255)
                                                                                                                             aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGAAGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCACCAACCAGATATTCAGCGCCACGACCCGCACCACCATCACCCAGCCTGTCTGATTTACGCGttt                         >  1:203307/1‑150 (MQ=39)
                                                                                                                               ggCGGATGCGGCGTAAACGCCTTATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGc                       >  1:1464596/1‑150 (MQ=255)
                                                                                                                               ggCGGATAAGGCGTTTACGGCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGc                       <  2:1516981/150‑1 (MQ=255)
                                                                                                                                gCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGACCAGCACCACCATCCCCCGGCCTGTCTGTTTTACGCGTTTGCt                      >  1:1511130/1‑150 (MQ=255)
                                                                                                                                 cgtaTAAGGCGTTTACGCAGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTg                     <  2:1382324/147‑1 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCAcc               <  2:491558/150‑1 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCACACCAACAACCAGATATTCAGCTCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCAcc               >  2:33092/1‑150 (MQ=255)
                                                                                                                                        ggCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAAGCAGATATTCAGCGCCACGCCCAGCCCCCCAATCCCCCAGCCTGGCTGGTTTACGCGTGTGCTGtaccccc              >  1:344987/1‑144 (MQ=255)
                                                                                                                                         gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAg             <  1:1597713/150‑1 (MQ=255)
                                                                                                                                         gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAg             <  1:136261/150‑1 (MQ=255)
                                                                                                                                              tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGc        <  1:1728131/150‑1 (MQ=255)
                                                                                                                                                cgcctcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTTCAATCCCAGCGCCGTCCCCACCAACAACAAGATATTCTGCGACACGACCAGCACCACAATCCCCCAGCCTGTCTGTTTTACCGGTTTGCTGTTCACCAGCTCGccc      >  2:843502/1‑150 (MQ=255)
                                                                                                                                                cgccgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGccc      <  2:891021/150‑1 (MQ=255)
                                                                                                                                                    gcATCTGGCACTATTGGCGGATGCGGCGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCAACAACCAGATATTCAGCGCCACGCCCAGCACCACCATCACCCAGCCGGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCa  >  1:1596247/1‑150 (MQ=255)
                                                                                                                                                    gcATCTGGCACTATTGGCGGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCa  >  1:492815/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCA  >  NC_000913/2511283‑2511580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: