Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,225 T→A 6.1% intergenic (+20/+32) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2250TA 6.1% 349.7 / 8.7 124intergenic (+20/+32)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (49/66);  new base A (3/5);  total (53/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 124 total aligned reads displayed.

TATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCC  >  NC_000913/2519080‑2519368
                                                                                                                                                 |                                                                                                                                               
tATCGACAAATCATCAACGCGATAGATAATATGACTGACATTGATCAGGAAAAAGTCGCGCAATTTTTGTCACGTGATGGAATTCGCAATTTCCTCGAACGTCTCGATATTGAAGAATCAGCATAAATAAAAAACGGCAGGATATTAtct                                                                                                                                             <  2:1027437/150‑1 (MQ=255)
  tctaaaaaTCATCAACGCTATATATAATATGACAGCCATTGAGCAGGAAAACGTCGCGCAATTTTTGTCACGTGAAGCAATTCTCAATTTCCTCGCCCGTCTCTATATTCACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTcc                                                                                                                                           <  2:1695605/145‑1 (MQ=255)
    gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                         >  2:344753/1‑150 (MQ=255)
        aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGt                                                                                                                                     >  2:1568034/1‑150 (MQ=255)
        aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATATTAGTCACGTGAAGGAATTCGCAATTACCTCTCCCGTCTCGATATTGCCGAATCAGCATATTTAAAAAACGGCAGGATAGTATCTGCTGCGGt                                                                                                                                     >  1:226435/1‑150 (MQ=255)
           catcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGtttt                                                                                                                                  <  2:34715/150‑2 (MQ=255)
           catcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGACGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTa                                                                                                                                  >  1:1613572/1‑150 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTAt                                                                                                                                 >  2:816340/1‑150 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                 <  1:1172189/150‑3 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                 <  2:10588/150‑3 (MQ=255)
                aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                             >  1:1368287/1‑150 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                            <  2:1374819/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                            <  2:507329/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                            <  1:395454/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                            <  2:381261/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTACCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                            <  1:897938/150‑1 (MQ=255)
                  gcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                                                           <  2:1363535/150‑1 (MQ=255)
                      tagataAAATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTGTATCTTTTTaaac                                                                                                                       >  1:806566/1‑150 (MQ=255)
                      tagaaaATATGACAGACATTGAGCACGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATACGCAATATCCTCGCCCGTCTCGATATTGACGAATAAGCATAAATAAAAAACGGCAGGATATAATCACCTGACGTTTATATTTTTacaa                                                                                                                       >  1:806568/1‑149 (MQ=255)
                          taataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTa                                                                                                                   <  1:1088228/150‑1 (MQ=255)
                             tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAAtt                                                                                                                <  2:297845/150‑1 (MQ=255)
                                gacagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAct                                                                                                             >  2:517488/1‑150 (MQ=255)
                                 acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActg                                                                                                            <  2:808264/150‑1 (MQ=255)
                                 acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActg                                                                                                            >  1:485840/1‑150 (MQ=255)
                                   agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                          >  2:433396/1‑150 (MQ=255)
                                       aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGAAGGATATTATTTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtt                                                                                                      >  1:444998/1‑150 (MQ=255)
                                        ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAttt                                                                                                     <  1:435081/150‑1 (MQ=255)
                                         tGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt                                                                                                    <  2:909082/150‑1 (MQ=255)
                                         tGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt                                                                                                    <  1:514096/150‑1 (MQ=255)
                                          gAGCAGGAAAAAGTCGCGAAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                                                                   <  1:1350415/150‑1 (MQ=255)
                                           aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCTCAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                  <  2:1544204/150‑1 (MQ=255)
                                           aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                  <  1:1724143/150‑1 (MQ=255)
                                             cAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                <  1:991301/150‑1 (MQ=255)
                                             cAGGAAAAAGTCGCGCAATTTTTGTACCGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                <  1:1272778/150‑1 (MQ=255)
                                               ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                                                                              >  2:1537128/1‑150 (MQ=255)
                                                  aaaaGTCGCTCAATTTTTGTCACGTTAAGGAATTCTCAATTTCCTCGCCCGTGTCGATATTTACGAATCAGCATAAATAAAACACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCTTTCAg                                                                                           <  2:1551641/150‑1 (MQ=255)
                                                   aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAACAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                          <  2:825847/150‑1 (MQ=255)
                                                       tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATa                                                                                      <  1:528209/150‑1 (MQ=255)
                                                       tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATa                                                                                      <  2:1368287/150‑1 (MQ=255)
                                                       tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCAAAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATa                                                                                      <  1:163544/150‑1 (MQ=255)
                                                        cgcgCATTTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTATCTATTTTGAAGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa                                                                                     <  1:1097018/150‑1 (MQ=255)
                                                        cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa                                                                                     <  1:894129/150‑1 (MQ=255)
                                                          cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAAACAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                                   >  2:245137/1‑150 (MQ=255)
                                                           gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt                                                                                  >  1:1621906/1‑150 (MQ=255)
                                                            cAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAta                                                                                 >  2:1318772/1‑149 (MQ=255)
                                                             aaTTTTTGTCACGTGAAGGAATTCGAAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATcc                                                                                <  1:1112746/150‑1 (MQ=255)
                                                               tttttGTCACGAGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACCAATCAGCATAAATAAAAAACGTCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCAAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg                                                                              <  1:250657/150‑1 (MQ=255)
                                                                 tttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                                                                            <  1:1587240/150‑1 (MQ=255)
                                                                       aCGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgt                                                                      >  2:1147177/1‑150 (MQ=255)
                                                                        cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATAGTGACGAATCAGCATAAATAAAATACGGCAGGATATGAGCGGCGGCCGTTGCTCTTTTTACACGCAAATTACTGCTGATTTTCGCGGTCAGCAATCAACTCCCGCGCgttgtt                                                                     >  2:1041678/1‑150 (MQ=255)
                                                                           gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                  >  2:38451/1‑150 (MQ=255)
                                                                           gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                  >  1:40665/1‑150 (MQ=255)
                                                                            aaGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGAAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGCTa                                                                 >  1:69257/1‑150 (MQ=255)
                                                                            aaGGAATTCGCAATTTCCTCGACCGTCTCGATATTGAAGAATCATCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa                                                                 <  1:405266/150‑1 (MQ=255)
                                                                             aGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATAc                                                                >  1:540784/1‑150 (MQ=255)
                                                                             aGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATAc                                                                >  1:1093577/1‑150 (MQ=255)
                                                                              ggAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACg                                                               >  2:453834/1‑150 (MQ=255)
                                                                                aaTTCGCAATTTCCTCGCCCGCCTCGATATTCACGAATCAGCATAACTAACAACCGGCAGGATATTATCTCCTGCCGATTATCTTTTTACACGCTACTTACTGCTGATTTTCGCGGTCAGCAATAAAATCCAGCGCTTTGTTGATACGcc                                                             <  1:172821/150‑2 (MQ=255)
                                                                                 aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACAAGATAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                                                            >  1:1412744/1‑150 (MQ=255)
                                                                                  ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                           >  2:702450/1‑150 (MQ=255)
                                                                                  ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                           <  2:888934/150‑1 (MQ=255)
                                                                                    cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                         >  2:1127021/1‑150 (MQ=255)
                                                                                    cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                         >  1:527022/1‑150 (MQ=255)
                                                                                    cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTGTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTAAGCAATAAAATCCAGCCCGTTGTTTATACGCTCGAt                                                         >  1:1085160/1‑150 (MQ=255)
                                                                                     gCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTATTTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATa                                                        >  1:433246/1‑150 (MQ=255)
                                                                                       aaTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACt                                                      >  1:1665537/1‑150 (MQ=255)
                                                                                         tttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGc                                                    <  1:1147177/150‑1 (MQ=255)
                                                                                         tttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGc                                                    >  2:1293569/1‑150 (MQ=255)
                                                                                          ttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                                   <  2:1621906/150‑1 (MQ=255)
                                                                                          ttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                                   <  2:1345890/150‑1 (MQ=255)
                                                                                             cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                >  1:629967/1‑150 (MQ=255)
                                                                                             cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                >  1:408897/1‑150 (MQ=255)
                                                                                              gcGCCCGTCTCTAAATTTAAGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTc                                               <  1:813432/149‑1 (MQ=255)
                                                                                                gCCCGTCACGAATTTGCAGTATCAGCAAAAATAAAACACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCtt                                             <  1:677605/150‑1 (MQ=255)
                                                                                                   cGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAcc                                          <  2:1390662/150‑1 (MQ=255)
                                                                                                    gTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCa                                         <  2:621162/150‑1 (MQ=255)
                                                                                                        cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTGCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                     <  2:713062/150‑1 (MQ=255)
                                                                                                           tatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGt                                  <  1:1568034/150‑1 (MQ=255)
                                                                                                            ttagACGAATCAGAATAAATAAAAAACGGCAGGATATTATTTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                 <  2:444998/147‑1 (MQ=255)
                                                                                                            atTGACGAATCAGCATAAATAAAAAACGGCATGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                 <  2:862395/150‑1 (MQ=255)
                                                                                                            atTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCAAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                 >  2:1733517/1‑150 (MQ=255)
                                                                                                                aCGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACg                             <  1:970912/150‑1 (MQ=255)
                                                                                                                    aTCCGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTaa                         <  1:182096/150‑1 (MQ=255)
                                                                                                                    aTCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTaa                         <  1:1200667/150‑1 (MQ=255)
                                                                                                                      cAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACa                       >  2:529976/1‑150 (MQ=255)
                                                                                                                       aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACAt                      <  2:1613572/150‑1 (MQ=255)
                                                                                                                         cATAAATAAAAAACGTCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATcc                    <  2:796955/150‑1 (MQ=255)
                                                                                                                         aataaataaAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATcc                    <  1:642593/149‑1 (MQ=255)
                                                                                                                          ataaataaAAACCGGGATGATCTTATATCCTGCCGTTTATCTTTTTACACGCTAAATACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTTACATCCa                   <  2:226435/150‑1 (MQ=255)
                                                                                                                            aaataaaAAACGGCAGGATATTATCTCCTGCCGATTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCCGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGt                 >  2:913470/1‑150 (MQ=255)
                                                                                                                              ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTga               >  1:875941/1‑149 (MQ=255)
                                                                                                                                aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg             <  2:427677/150‑1 (MQ=255)
                                                                                                                                 aaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTgg            <  2:629967/150‑1 (MQ=255)
                                                                                                                                 aaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTgg            >  1:1350879/1‑150 (MQ=255)
                                                                                                                                   aaaCGGCAGGATATTATCTCGTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag          <  1:645142/150‑1 (MQ=255)
                                                                                                                                    aaCGGCAGGAGATAATATCCTGCCGTTTTTCTTTTTACACGCAAATTACTGCGGAATATCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACCCTCGATAATGCCGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga         >  1:845191/1‑150 (MQ=255)
                                                                                                                                      cGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGAca       >  1:348739/1‑149 (MQ=255)
                                                                                                                                        gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGc     <  1:1537128/150‑1 (MQ=255)
                                                                                                                                        gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGc     >  2:491550/1‑150 (MQ=255)
                                                                                                                                           ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcccc  >  2:1603548/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCC  >  NC_000913/2519080‑2519368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: