Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,228 C→A 5.7% intergenic (+23/+29) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2280CA 5.7% 363.7 / 3.6 125intergenic (+23/+29)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (50/64);  new base A (3/6);  total (54/71)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGT  >  NC_000913/2519080‑2519376
                                                                                                                                                    |                                                                                                                                                    
   cGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCt                                                                                                                                                  <  1:1616428/150‑1 (MQ=255)
    gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                                 >  2:344753/1‑150 (MQ=255)
        aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATATTAGTCACGTGAAGGAATTCGCAATTACCTCTCCCGTCTCGATATTGCCGAATCAGCATATTTAAAAAACGGCAGGATAGTATCTGCTGCGGt                                                                                                                                             >  1:226435/1‑150 (MQ=255)
           catcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGtttt                                                                                                                                          <  2:34715/150‑2 (MQ=255)
           catcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGACGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTa                                                                                                                                          >  1:1613572/1‑150 (MQ=255)
            atcaAGGGGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCATGAGATAATATCCTGCCGttttt                                                                                                                                         <  1:685784/150‑3 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTAt                                                                                                                                         >  2:816340/1‑150 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                         <  2:10588/150‑3 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                         <  1:1172189/150‑3 (MQ=255)
            atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                         <  2:1667282/150‑3 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                                    <  2:1374819/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                                    <  1:395454/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                                    <  2:381261/150‑1 (MQ=255)
                 cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                                    <  2:507329/150‑1 (MQ=255)
                      tagataAAATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTGTATCTTTTTaaac                                                                                                                               >  1:806566/1‑150 (MQ=255)
                      tagaaaATATGACAGACATTGAGCACGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATACGCAATATCCTCGCCCGTCTCGATATTGACGAATAAGCATAAATAAAAAACGGCAGGATATAATCACCTGACGTTTATATTTTTacaa                                                                                                                               >  1:806568/1‑149 (MQ=255)
                          taataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTa                                                                                                                           <  1:1088228/150‑1 (MQ=255)
                               tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTTAATGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                                      <  1:92380/150‑1 (MQ=255)
                               tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTaa                                                                                                                      >  2:7142/1‑149 (MQ=255)
                                 acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActg                                                                                                                    <  2:808264/150‑1 (MQ=255)
                                 acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActg                                                                                                                    >  1:485840/1‑150 (MQ=255)
                                   agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                                  >  2:433396/1‑150 (MQ=255)
                                       aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGAAGGATATTATTTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtt                                                                                                              >  1:444998/1‑150 (MQ=255)
                                        ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAttt                                                                                                             <  1:435081/150‑1 (MQ=255)
                                         tGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt                                                                                                            <  2:909082/150‑1 (MQ=255)
                                         tGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt                                                                                                            <  1:514096/150‑1 (MQ=255)
                                          gAGCAGGAAAAAGTCGCGAAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                                                                           <  1:1350415/150‑1 (MQ=255)
                                           aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCTCAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                          <  2:1544204/150‑1 (MQ=255)
                                           aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                          <  1:1724143/150‑1 (MQ=255)
                                             cAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                        <  1:344753/150‑1 (MQ=255)
                                             cAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                        <  1:991301/150‑1 (MQ=255)
                                             cAGGAAAAAGTCGCGCAATTTTTGTACCGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                        <  1:1272778/150‑1 (MQ=255)
                                               ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                                                                                      >  2:1537128/1‑150 (MQ=255)
                                                  aaaaGTCGCTCAATTTTTGTCACGTTAAGGAATTCTCAATTTCCTCGCCCGTGTCGATATTTACGAATCAGCATAAATAAAACACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCTTTCAg                                                                                                   <  2:1551641/150‑1 (MQ=255)
                                                  aaaaGTCGCGAAATTTTTGTCACTTTATGTAATTCGCAATTTCCTCGCCGTTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAg                                                                                                   <  1:1457118/150‑1 (MQ=255)
                                                   aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAACAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                                  <  2:825847/150‑1 (MQ=255)
                                                   aaaGTCGCGCAATTTTTGACACGTGAAGGAATTCGAAATTTCCTCGCCGGTCTCGATATTGACGAATCAGCATGAATAAAAAACGGCAGGATATTTTCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                                  <  1:1728052/150‑1 (MQ=255)
                                                      gTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCaaa                                                                                               >  2:1322901/1‑149 (MQ=255)
                                                       tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATa                                                                                              <  2:1368287/150‑1 (MQ=255)
                                                       tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATa                                                                                              <  1:528209/150‑1 (MQ=255)
                                                        cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa                                                                                             <  1:894129/150‑1 (MQ=255)
                                                          cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAAACAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                                           >  2:245137/1‑150 (MQ=255)
                                                           gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt                                                                                          >  1:1345890/1‑150 (MQ=255)
                                                           gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt                                                                                          >  1:1621906/1‑150 (MQ=255)
                                                            cAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAta                                                                                         >  2:1318772/1‑149 (MQ=255)
                                                             aaTTTTTGTCACGTGAAGGAATTCGAAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATcc                                                                                        <  1:1112746/150‑1 (MQ=255)
                                                               tttttGTCACGAGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACCAATCAGCATAAATAAAAAACGTCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCAAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg                                                                                      <  1:250657/150‑1 (MQ=255)
                                                                 tttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg                                                                                    <  1:1587240/150‑1 (MQ=255)
                                                                        cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATATATAAAAAACGGCGGGATATTATCTCCTGCCGTTTATATTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                             >  2:836403/1‑150 (MQ=255)
                                                                        cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATAGTGACGAATCAGCATAAATAAAATACGGCAGGATATGAGCGGCGGCCGTTGCTCTTTTTACACGCAAATTACTGCTGATTTTCGCGGTCAGCAATCAACTCCCGCGCgttgtt                                                                             >  2:1041678/1‑150 (MQ=255)
                                                                          ttaaGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa                                                                           <  1:819139/148‑1 (MQ=255)
                                                                           gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                          >  1:40665/1‑150 (MQ=255)
                                                                            aaGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGAAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGCTa                                                                         >  1:69257/1‑150 (MQ=255)
                                                                            aaGGAATTCGCAATTTCCTCGACCGTCTCGATATTGAAGAATCATCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa                                                                         <  1:405266/150‑1 (MQ=255)
                                                                             aGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATAc                                                                        >  1:540784/1‑150 (MQ=255)
                                                                             aGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATAc                                                                        >  1:1093577/1‑150 (MQ=255)
                                                                              ggAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACg                                                                       >  2:453834/1‑150 (MQ=255)
                                                                                aaTTCGCAATTTCCTCGCCCGCCTCGATATTCACGAATCAGCATAACTAACAACCGGCAGGATATTATCTCCTGCCGATTATCTTTTTACACGCTACTTACTGCTGATTTTCGCGGTCAGCAATAAAATCCAGCGCTTTGTTGATACGcc                                                                     <  1:172821/150‑2 (MQ=255)
                                                                                 aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACAAGATAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                                                                    >  1:1412744/1‑150 (MQ=255)
                                                                                  ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                                   >  2:398894/1‑150 (MQ=255)
                                                                                  ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                                   <  2:888934/150‑1 (MQ=255)
                                                                                  ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                                   <  2:55127/150‑1 (MQ=255)
                                                                                  ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                                   >  2:702450/1‑150 (MQ=255)
                                                                                    cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                                 >  2:1127021/1‑150 (MQ=255)
                                                                                    cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                                 >  1:527022/1‑150 (MQ=255)
                                                                                    cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTGTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTAAGCAATAAAATCCAGCCCGTTGTTTATACGCTCGAt                                                                 >  1:1085160/1‑150 (MQ=255)
                                                                                     gCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTATTTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATa                                                                >  1:433246/1‑150 (MQ=255)
                                                                                       aaTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACt                                                              >  1:1665537/1‑150 (MQ=255)
                                                                                         tttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGc                                                            <  1:1147177/150‑1 (MQ=255)
                                                                                         tttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGc                                                            >  2:1293569/1‑150 (MQ=255)
                                                                                          ttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                                           <  2:1621906/150‑1 (MQ=255)
                                                                                          ttCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                                           <  2:1345890/150‑1 (MQ=255)
                                                                                             cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                        >  1:629967/1‑150 (MQ=255)
                                                                                             cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                        >  1:408897/1‑150 (MQ=255)
                                                                                               cGCCCGTCTCGATATTGACGAATCAGCAAAAATAAAAAACGGCAGGAAATTATATCCAGCCGTTTATCATTTTACACGCAAATTACTGCTGATTTTCGCGTTCAGCAATAAAATACAACGCTTTGTATATACGCTCGATACAGAGGGGCt                                                      >  2:285373/1‑150 (MQ=255)
                                                                                                gCCCGTCTCGATATTGACGAATCAGCATAATTAAAAAACGGCAGGATATTATCTCCTGGCGTATATCTTTTTACACGCTAATTACTTCTGATTTTCGCGTTCAGCAATAATATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCtt                                                     <  2:1388200/150‑1 (MQ=255)
                                                                                                gCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCtt                                                     <  2:1073607/150‑1 (MQ=255)
                                                                                                        cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTGCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                             <  2:713062/150‑1 (MQ=255)
                                                                                                            atTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCAAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                         >  2:1733517/1‑150 (MQ=255)
                                                                                                             ttGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGa                                        >  2:155748/1‑150 (MQ=255)
                                                                                                               gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAAc                                      <  1:1127021/150‑1 (MQ=255)
                                                                                                               gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAAc                                      >  2:465875/1‑150 (MQ=255)
                                                                                                                aCGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACg                                     <  1:970912/150‑1 (MQ=255)
                                                                                                                      cAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACa                               >  2:529976/1‑150 (MQ=255)
                                                                                                                       aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACAt                              <  2:1613572/150‑1 (MQ=255)
                                                                                                                         aataaataaAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATcc                            <  1:642593/149‑1 (MQ=255)
                                                                                                                          ataaataaAAACCGGGATGATCTTATATCCTGCCGTTTATCTTTTTACACGCTAAATACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTTACATCCa                           <  2:226435/150‑1 (MQ=255)
                                                                                                                            aaataaaAAACGGCAGGATATTATCTCCTGCCGATTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCCGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGt                         >  2:913470/1‑150 (MQ=255)
                                                                                                                              ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTga                       >  1:875941/1‑149 (MQ=255)
                                                                                                                                aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg                     <  2:427677/150‑1 (MQ=255)
                                                                                                                                 aaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTgg                    >  1:1350879/1‑150 (MQ=255)
                                                                                                                                 aaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTgg                    <  2:629967/150‑1 (MQ=255)
                                                                                                                                   aaaCGGCAGGATATTATCTCGTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag                  <  1:645142/150‑1 (MQ=255)
                                                                                                                                    aaCGGCAGGAGATAATATCCTGCCGTTTTTCTTTTTACACGCAAATTACTGCGGAATATCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACCCTCGATAATGCCGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga                 >  1:845191/1‑150 (MQ=255)
                                                                                                                                        gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGc             >  2:491550/1‑150 (MQ=255)
                                                                                                                                        gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGc             <  1:1537128/150‑1 (MQ=255)
                                                                                                                                           ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcccc          >  2:1603548/1‑150 (MQ=255)
                                                                                                                                                  atCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCCGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCgg   >  2:44254/1‑150 (MQ=255)
                                                                                                                                                  atCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCgg   <  2:527022/150‑1 (MQ=255)
                                                                                                                                                   tctcCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGt  <  1:1094277/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGT  >  NC_000913/2519080‑2519376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: