Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,526,241 T→G 6.1% I206L (ATT→CTT) ‡ yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,526,2410TG 6.1% 272.5 / 6.3 92I206L (ATT→CTT) ‡yfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base T (44/42);  new base G (3/3);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

AGTGGGCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGA  >  NC_000913/2526093‑2526389
                                                                                                                                                    |                                                                                                                                                    
aGTGGGCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCAACGGGAACAGCGTCGCCGGATGACCAAACTCCTGCACAAAATTCGCCTGAATACAATTGCGCGCCAGCGCTGCGTCAAta                                                                                                                                                     >  2:438154/1‑149 (MQ=255)
   gggCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGc                                                                                                                                                  <  1:1155113/150‑1 (MQ=255)
     gCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCgg                                                                                                                                                >  2:454645/1‑150 (MQ=255)
     gCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCgg                                                                                                                                                >  1:109750/1‑150 (MQ=255)
        aCCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCg                                                                                                                                             <  1:1048633/150‑1 (MQ=255)
         ccTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCgg                                                                                                                                            >  1:1301295/1‑150 (MQ=255)
          cTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGc                                                                                                                                           <  1:969666/150‑1 (MQ=255)
              aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                       >  2:228561/1‑150 (MQ=255)
              aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                       >  2:1217936/1‑150 (MQ=255)
               ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCCGCGCTGCGTCAATCAGCGGTCGGCGGccg                                                                                                                                      >  1:1478138/1‑149 (MQ=255)
               ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                      >  1:1293410/1‑150 (MQ=255)
               ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                      <  1:438154/150‑1 (MQ=255)
                  aaTGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAc                                                                                                                                   <  1:1108192/150‑1 (MQ=255)
                      gTAGCGCCAGTGCGGGAAGAATACTGATGCCGCTGCCTGCGGCTACCAGCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGACTACCATTGCGCGCCAGCGCTGCGGCAGGCAGCCGACCGCTGATTGAcgcag                                                                                                                               <  1:1506656/150‑3 (MQ=255)
                       tAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAcgcagc                                                                                                                              <  1:1660870/150‑4 (MQ=255)
                         gcgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAAt                                                                                                                            <  2:118099/150‑1 (MQ=255)
                          cgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATc                                                                                                                           >  1:1414106/1‑150 (MQ=255)
                           gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                          <  2:1243763/150‑1 (MQ=255)
                           gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                          <  2:1243765/150‑1 (MQ=255)
                                 gCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAac                                                                                                                    <  1:603126/150‑1 (MQ=255)
                                   gggAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAacac                                                                                                                  <  1:312482/150‑1 (MQ=255)
                                      aagaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCaa                                                                                                               >  2:1507251/1‑150 (MQ=255)
                                        gaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAAGCTCCTGCCCAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAtt                                                                                                             >  2:302323/1‑150 (MQ=255)
                                         aaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGACGGATGTCCAAACTCCTGCACAATATTCGCATGAATACCATTGAGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAttt                                                                                                            >  1:668704/1‑150 (MQ=255)
                                           tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                          <  2:1205243/150‑1 (MQ=255)
                                           tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                          <  2:149769/150‑1 (MQ=255)
                                            aCTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTTAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGc                                                                                                         <  2:1397517/150‑1 (MQ=255)
                                             cTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCt                                                                                                        >  1:461522/1‑150 (MQ=255)
                                                  gccgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTg                                                                                                   <  1:1731234/150‑1 (MQ=255)
                                                    cgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTa                                                                                                 >  2:1400305/1‑150 (MQ=255)
                                                      ctgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCTGCTGCCTGACGCGTAAGCCAGCAACACCAATTTCGCTCCCTGTAAt                                                                                               <  1:266127/149‑1 (MQ=255)
                                                      atgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAt                                                                                               <  1:852093/150‑1 (MQ=255)
                                                         ccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCAAAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcc                                                                                            >  2:495927/1‑150 (MQ=255)
                                                          cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                           <  1:109868/150‑1 (MQ=255)
                                                          cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                           <  1:1231835/150‑1 (MQ=255)
                                                          cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                           <  1:525740/150‑1 (MQ=255)
                                                            gCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgc                                                                                         >  1:1645842/1‑150 (MQ=255)
                                                             cGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgcc                                                                                        <  2:1293410/150‑1 (MQ=255)
                                                               gCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAg                                                                                      >  2:216998/1‑150 (MQ=255)
                                                                  aCCATCTGGAACAGCTTAGCCGGATGTCCAATCTCCTGCACAATATTCGCATGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCCACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGt                                                                                   <  2:470118/150‑1 (MQ=255)
                                                                   ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                  >  2:604935/1‑150 (MQ=255)
                                                                   ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                  >  2:749787/1‑150 (MQ=255)
                                                                    cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                 <  1:1346843/150‑1 (MQ=255)
                                                                    cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                 <  1:1006600/150‑1 (MQ=255)
                                                                     aTCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACa                                                                                <  1:1122635/150‑1 (MQ=255)
                                                                        gggAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATaa                                                                             <  1:1009359/150‑1 (MQ=255)
                                                                          gAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATc                                                                           >  2:1321290/1‑150 (MQ=255)
                                                                             cAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTc                                                                        <  1:599577/150‑1 (MQ=255)
                                                                              aGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTcc                                                                       <  1:862165/150‑1 (MQ=255)
                                                                                         aTGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAgc                                                            <  1:1246360/150‑1 (MQ=255)
                                                                                            tCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCt                                                         >  1:706520/1‑150 (MQ=255)
                                                                                             ccAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTa                                                        >  1:651769/1‑150 (MQ=255)
                                                                                                 tctcCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTGAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCGTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATcgc                                                    <  2:1155193/150‑1 (MQ=255)
                                                                                                    ccTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGAt                                                 <  1:573309/150‑1 (MQ=255)
                                                                                                      tGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATgc                                               >  2:966116/1‑149 (MQ=255)
                                                                                                       gCACAATATTCGCCTGAATTCCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCAGCCGCGTAATCCTGCAACTCCAATGTCGCTCCCTGTAATGCCGGCCAGGGGAGATTAGCTGACCCGGCCAAGGCGGTATCCCGATGGc                                              >  2:940955/1‑150 (MQ=255)
                                                                                                         acaATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCaa                                            <  2:1143354/150‑1 (MQ=255)
                                                                                                            atatTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGc                                         >  1:1448188/1‑150 (MQ=255)
                                                                                                              atTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCag                                       <  1:604935/150‑1 (MQ=255)
                                                                                                                  gCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCagaaag                                   >  2:1492103/1‑150 (MQ=255)
                                                                                                                     tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCCCGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                >  1:1055322/1‑150 (MQ=255)
                                                                                                                     tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                <  1:1507251/150‑1 (MQ=255)
                                                                                                                      gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTACTGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                               >  1:140395/1‑150 (MQ=255)
                                                                                                                      gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGATCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                               >  2:85379/1‑150 (MQ=255)
                                                                                                                      gAATAACATTGCGAGACAGCTCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCATGCAACACCAATTTCGCTCCCTGTAATGCCTGACAGGGTACATAATCTTCCACGTCCAAAGCGCTATCGCGATGGCAAATCAGaaagaaag                               >  2:1565696/1‑150 (MQ=255)
                                                                                                                        aTACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGc                             <  1:978459/150‑1 (MQ=255)
                                                                                                                           ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                          >  2:303458/1‑150 (MQ=255)
                                                                                                                           ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                          >  2:676034/1‑150 (MQ=255)
                                                                                                                           ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                          >  2:1539357/1‑150 (MQ=255)
                                                                                                                           ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                          <  1:92226/150‑1 (MQ=255)
                                                                                                                            cATTGCGCGCCAGCGCTGCGGCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCgg                         <  1:1400305/150‑1 (MQ=255)
                                                                                                                             aTTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGa                        >  1:62131/1‑150 (MQ=255)
                                                                                                                                   cgcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAAt                  >  1:1253385/1‑150 (MQ=255)
                                                                                                                                    gcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATc                 <  2:1600212/150‑1 (MQ=255)
                                                                                                                                     ccAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCg                >  2:1600875/1‑150 (MQ=255)
                                                                                                                                       aGCGCTGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt              <  2:1259251/150‑1 (MQ=255)
                                                                                                                                       aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCACCACCAATTTCGCTCGCTGTAATGCCTACCAGGGTACATAATCTTCCACGGCAAAAGCGCTATCGCGATGGCAAACCAAAAAGAAAGGATACGAAAGaaactct              >  2:93807/1‑145 (MQ=255)
                                                                                                                                       aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGATAGGCTCGGAAAGAATCGCt              <  1:155147/150‑1 (MQ=255)
                                                                                                                                       aGCGCGGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt              <  2:864583/150‑1 (MQ=255)
                                                                                                                                           acgCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcac          >  2:1642149/3‑150 (MQ=255)
                                                                                                                                            tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTTTTATGCATGCGTGAGTACATAATCTTCAACTGACAAAGAGCTATCTCGATGGCAAAGCTGAAAGAATGGCTCGGAATGAATCGGTTcaca         >  2:1148810/1‑150 (MQ=255)
                                                                                                                                            tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAACGGCTCGGAAATAATCGCTTcaca         >  2:1589904/1‑150 (MQ=255)
                                                                                                                                            tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca         <  2:461522/150‑1 (MQ=255)
                                                                                                                                            tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca         >  2:1025216/1‑150 (MQ=255)
                                                                                                                                             gCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGGGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAt        >  2:279429/1‑150 (MQ=255)
                                                                                                                                              cGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt       >  1:372793/1‑150 (MQ=255)
                                                                                                                                              cGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt       >  2:1519648/1‑150 (MQ=255)
                                                                                                                                               gTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTg      >  2:218915/1‑150 (MQ=255)
                                                                                                                                                tCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCTCTCCCTGTAATGCCTTCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATTGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGc     <  2:668704/150‑1 (MQ=255)
                                                                                                                                                 cAATCAGCGGTCGGCTGCCTGACGCGTAGTCCTGCCAGCCCAGTTTCGCTGCCTGTAATGCCTGCCAGGGTGCATACTCTGCCACGGCCAAAGCGCTATCGCGAGGGCAAAGCAGAAAGAAAGGCTCGGAAAGCATCGCTTCACATTGcc    >  2:979273/1‑149 (MQ=255)
                                                                                                                                                 cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa    >  1:722391/1‑150 (MQ=255)
                                                                                                                                                 cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAGTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTACCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa    <  1:1040305/150‑1 (MQ=255)
                                                                                                                                                   aTCAGCGGTCCGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACAAAATCTTCCACGGCCAAAGCGCTATCGCGAAGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCATCACATTGCAGa  >  1:228165/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGTGGGCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGA  >  NC_000913/2526093‑2526389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: