Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,526,246 G→C 6.4% P204R (CCG→CGG)  yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,526,2460GC 6.4% 288.1 / 9.8 94P204R (CCG→CGG) yfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base G (44/42);  new base C (3/3);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCC  >  NC_000913/2526098‑2526394
                                                                                                                                                    |                                                                                                                                                    
gCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCgg                                                                                                                                                     >  2:454645/1‑150 (MQ=255)
gCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCgg                                                                                                                                                     >  1:109750/1‑150 (MQ=255)
   aCCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCg                                                                                                                                                  <  1:1048633/150‑1 (MQ=255)
    ccTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCgg                                                                                                                                                 >  1:1301295/1‑150 (MQ=255)
     cTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGc                                                                                                                                                <  1:969666/150‑1 (MQ=255)
         aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                            >  2:1217936/1‑150 (MQ=255)
         aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                            >  2:228561/1‑150 (MQ=255)
          ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCCGCGCTGCGTCAATCAGCGGTCGGCGGccg                                                                                                                                           >  1:1478138/1‑149 (MQ=255)
          ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                           <  1:438154/150‑1 (MQ=255)
          ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                           >  1:1293410/1‑150 (MQ=255)
             aaTGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAc                                                                                                                                        <  1:1108192/150‑1 (MQ=255)
                 gTAGCGCCAGTGCGGGAAGAATACTGATGCCGCTGCCTGCGGCTACCAGCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGACTACCATTGCGCGCCAGCGCTGCGGCAGGCAGCCGACCGCTGATTGAcgcag                                                                                                                                    <  1:1506656/150‑3 (MQ=255)
                  tAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAcgcagc                                                                                                                                   <  1:1660870/150‑4 (MQ=255)
                    gcgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAAt                                                                                                                                 <  2:118099/150‑1 (MQ=255)
                     cgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATc                                                                                                                                >  1:1414106/1‑150 (MQ=255)
                      gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                               <  2:1243765/150‑1 (MQ=255)
                      gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                               <  2:1243763/150‑1 (MQ=255)
                            gCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAac                                                                                                                         <  1:603126/150‑1 (MQ=255)
                              gggAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAacac                                                                                                                       <  1:312482/150‑1 (MQ=255)
                                 aagaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCaa                                                                                                                    >  2:1507251/1‑150 (MQ=255)
                                   gaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAAGCTCCTGCCCAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAtt                                                                                                                  >  2:302323/1‑150 (MQ=255)
                                    aaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGACGGATGTCCAAACTCCTGCACAATATTCGCATGAATACCATTGAGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAttt                                                                                                                 >  1:668704/1‑150 (MQ=255)
                                      tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                               <  2:149769/150‑1 (MQ=255)
                                      tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                               <  2:1205243/150‑1 (MQ=255)
                                       aCTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTTAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGc                                                                                                              <  2:1397517/150‑1 (MQ=255)
                                        cTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCt                                                                                                             >  1:461522/1‑150 (MQ=255)
                                             gccgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTg                                                                                                        <  1:1731234/150‑1 (MQ=255)
                                               cgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTa                                                                                                      >  2:1400305/1‑150 (MQ=255)
                                                 ctgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCTGCTGCCTGACGCGTAAGCCAGCAACACCAATTTCGCTCCCTGTAAt                                                                                                    <  1:266127/149‑1 (MQ=255)
                                                 atgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAt                                                                                                    <  1:852093/150‑1 (MQ=255)
                                                    ccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCAAAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcc                                                                                                 >  2:495927/1‑150 (MQ=255)
                                                     cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                <  1:109868/150‑1 (MQ=255)
                                                     cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                <  1:525740/150‑1 (MQ=255)
                                                     cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                <  1:1231835/150‑1 (MQ=255)
                                                       gCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgc                                                                                              >  1:1645842/1‑150 (MQ=255)
                                                        cGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgcc                                                                                             <  2:1293410/150‑1 (MQ=255)
                                                          gCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAg                                                                                           >  2:216998/1‑150 (MQ=255)
                                                             aCCATCTGGAACAGCTTAGCCGGATGTCCAATCTCCTGCACAATATTCGCATGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCCACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGt                                                                                        <  2:470118/150‑1 (MQ=255)
                                                              ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                       >  2:749787/1‑150 (MQ=255)
                                                              ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                       >  2:604935/1‑150 (MQ=255)
                                                               cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                      <  1:1346843/150‑1 (MQ=255)
                                                               cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                      <  1:1006600/150‑1 (MQ=255)
                                                                aTCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACa                                                                                     <  1:1122635/150‑1 (MQ=255)
                                                                   gggAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATaa                                                                                  <  1:1009359/150‑1 (MQ=255)
                                                                     gAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATc                                                                                >  2:1321290/1‑150 (MQ=255)
                                                                        cAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTc                                                                             <  1:599577/150‑1 (MQ=255)
                                                                         aGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTcc                                                                            <  1:862165/150‑1 (MQ=255)
                                                                                    aTGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAgc                                                                 <  1:1246360/150‑1 (MQ=255)
                                                                                       tCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCt                                                              >  1:706520/1‑150 (MQ=255)
                                                                                        ccAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTa                                                             >  1:651769/1‑150 (MQ=255)
                                                                                            tctcCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTGAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCGTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATcgc                                                         <  2:1155193/150‑1 (MQ=255)
                                                                                               ccTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGAt                                                      <  1:573309/150‑1 (MQ=255)
                                                                                                 tGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATgc                                                    >  2:966116/1‑149 (MQ=255)
                                                                                                  gCACAATATTCGCCTGAATTCCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCAGCCGCGTAATCCTGCAACTCCAATGTCGCTCCCTGTAATGCCGGCCAGGGGAGATTAGCTGACCCGGCCAAGGCGGTATCCCGATGGc                                                   >  2:940955/1‑150 (MQ=255)
                                                                                                    acaATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCaa                                                 <  2:1143354/150‑1 (MQ=255)
                                                                                                       atatTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGc                                              >  1:1448188/1‑150 (MQ=255)
                                                                                                         atTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCag                                            <  1:604935/150‑1 (MQ=255)
                                                                                                             gCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCagaaag                                        >  2:1492103/1‑150 (MQ=255)
                                                                                                                tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCCCGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                     >  1:1055322/1‑150 (MQ=255)
                                                                                                                tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                     <  1:1507251/150‑1 (MQ=255)
                                                                                                                 gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTACTGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                                    >  1:140395/1‑150 (MQ=255)
                                                                                                                 gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGATCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                                    >  2:85379/1‑150 (MQ=255)
                                                                                                                 gAATAACATTGCGAGACAGCTCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCATGCAACACCAATTTCGCTCCCTGTAATGCCTGACAGGGTACATAATCTTCCACGTCCAAAGCGCTATCGCGATGGCAAATCAGaaagaaag                                    >  2:1565696/1‑150 (MQ=255)
                                                                                                                   aTACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGc                                  <  1:978459/150‑1 (MQ=255)
                                                                                                                      ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                               >  2:303458/1‑150 (MQ=255)
                                                                                                                      ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                               >  2:676034/1‑150 (MQ=255)
                                                                                                                      ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                               >  2:1539357/1‑150 (MQ=255)
                                                                                                                      ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                               <  1:92226/150‑1 (MQ=255)
                                                                                                                       cATTGCGCGCCAGCGCTGCGGCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCgg                              <  1:1400305/150‑1 (MQ=255)
                                                                                                                        aTTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGa                             >  1:62131/1‑150 (MQ=255)
                                                                                                                              cgcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAAt                       >  1:1253385/1‑150 (MQ=255)
                                                                                                                               gcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATc                      <  2:1600212/150‑1 (MQ=255)
                                                                                                                                ccAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCg                     >  2:1600875/1‑150 (MQ=255)
                                                                                                                                  aGCGCTGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt                   <  2:1259251/150‑1 (MQ=255)
                                                                                                                                  aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCACCACCAATTTCGCTCGCTGTAATGCCTACCAGGGTACATAATCTTCCACGGCAAAAGCGCTATCGCGATGGCAAACCAAAAAGAAAGGATACGAAAGaaactct                   >  2:93807/1‑145 (MQ=255)
                                                                                                                                  aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGATAGGCTCGGAAAGAATCGCt                   <  1:155147/150‑1 (MQ=255)
                                                                                                                                  aGCGCGGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt                   <  2:864583/150‑1 (MQ=255)
                                                                                                                                      acgCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcac               >  2:1642149/3‑150 (MQ=255)
                                                                                                                                       tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTTTTATGCATGCGTGAGTACATAATCTTCAACTGACAAAGAGCTATCTCGATGGCAAAGCTGAAAGAATGGCTCGGAATGAATCGGTTcaca              >  2:1148810/1‑150 (MQ=255)
                                                                                                                                       tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAACGGCTCGGAAATAATCGCTTcaca              >  2:1589904/1‑150 (MQ=255)
                                                                                                                                       tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca              >  2:1025216/1‑150 (MQ=255)
                                                                                                                                       tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca              <  2:461522/150‑1 (MQ=255)
                                                                                                                                        gCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGGGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAt             >  2:279429/1‑150 (MQ=255)
                                                                                                                                         cGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt            >  1:372793/1‑150 (MQ=255)
                                                                                                                                         cGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt            >  2:1519648/1‑150 (MQ=255)
                                                                                                                                          gTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTg           >  2:218915/1‑150 (MQ=255)
                                                                                                                                           tCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCTCTCCCTGTAATGCCTTCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATTGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGc          <  2:668704/150‑1 (MQ=255)
                                                                                                                                            cAATCAGCGGTCGGCTGCCTGACGCGTAGTCCTGCCAGCCCAGTTTCGCTGCCTGTAATGCCTGCCAGGGTGCATACTCTGCCACGGCCAAAGCGCTATCGCGAGGGCAAAGCAGAAAGAAAGGCTCGGAAAGCATCGCTTCACATTGcc         >  2:979273/1‑149 (MQ=255)
                                                                                                                                            cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa         >  1:722391/1‑150 (MQ=255)
                                                                                                                                            cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAGTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTACCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa         <  1:1040305/150‑1 (MQ=255)
                                                                                                                                              aTCAGCGGTCCGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACAAAATCTTCCACGGCCAAAGCGCTATCGCGAAGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCATCACATTGCAGa       >  1:228165/1‑150 (MQ=255)
                                                                                                                                                cAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATc     <  2:1478138/150‑1 (MQ=255)
                                                                                                                                                cAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATc     <  1:1217936/150‑1 (MQ=255)
                                                                                                                                                 aGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCg    >  1:1573936/1‑150 (MQ=255)
                                                                                                                                                  gCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGc   >  2:254484/1‑150 (MQ=255)
                                                                                                                                                   cGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGcc  <  2:1085445/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCC  >  NC_000913/2526098‑2526394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: