Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,526,250 G→C 7.4% R203G (CGA→GGA) ‡ yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,526,2500GC 7.4% 284.9 / 10.0 92R203G (CGA→GGA) ‡yfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base G (42/42);  new base C (4/3);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTG  >  NC_000913/2526101‑2526399
                                                                                                                                                     |                                                                                                                                                     
aCCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCg                                                                                                                                                       <  1:1048633/150‑1 (MQ=255)
 ccTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCgg                                                                                                                                                      >  1:1301295/1‑150 (MQ=255)
  cTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGc                                                                                                                                                     <  1:969666/150‑1 (MQ=255)
      aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                                 >  2:1217936/1‑150 (MQ=255)
      aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                                 >  2:228561/1‑150 (MQ=255)
       ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCCGCGCTGCGTCAATCAGCGGTCGGCGGccg                                                                                                                                                >  1:1478138/1‑149 (MQ=255)
       ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                                <  1:438154/150‑1 (MQ=255)
       ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                                >  1:1293410/1‑150 (MQ=255)
          aaTGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAc                                                                                                                                             <  1:1108192/150‑1 (MQ=255)
              gTAGCGCCAGTGCGGGAAGAATACTGATGCCGCTGCCTGCGGCTACCAGCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGACTACCATTGCGCGCCAGCGCTGCGGCAGGCAGCCGACCGCTGATTGAcgcag                                                                                                                                         <  1:1506656/150‑3 (MQ=255)
               tAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAcgcagc                                                                                                                                        <  1:1660870/150‑4 (MQ=255)
                 gcgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAAt                                                                                                                                      <  2:118099/150‑1 (MQ=255)
                  cgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATc                                                                                                                                     >  1:1414106/1‑150 (MQ=255)
                   gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                                    <  2:1243765/150‑1 (MQ=255)
                   gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                                    <  2:1243763/150‑1 (MQ=255)
                         gCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAac                                                                                                                              <  1:603126/150‑1 (MQ=255)
                           gggAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAacac                                                                                                                            <  1:312482/150‑1 (MQ=255)
                              aagaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCaa                                                                                                                         >  2:1507251/1‑150 (MQ=255)
                                gaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAAGCTCCTGCCCAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAtt                                                                                                                       >  2:302323/1‑150 (MQ=255)
                                 aaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGACGGATGTCCAAACTCCTGCACAATATTCGCATGAATACCATTGAGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAttt                                                                                                                      >  1:668704/1‑150 (MQ=255)
                                   tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                                    <  2:149769/150‑1 (MQ=255)
                                   tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                                    <  2:1205243/150‑1 (MQ=255)
                                    aCTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTTAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGc                                                                                                                   <  2:1397517/150‑1 (MQ=255)
                                     cTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCt                                                                                                                  >  1:461522/1‑150 (MQ=255)
                                          gccgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTg                                                                                                             <  1:1731234/150‑1 (MQ=255)
                                            cgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTa                                                                                                           >  2:1400305/1‑150 (MQ=255)
                                              ctgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCTGCTGCCTGACGCGTAAGCCAGCAACACCAATTTCGCTCCCTGTAAt                                                                                                         <  1:266127/149‑1 (MQ=255)
                                              atgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAt                                                                                                         <  1:852093/150‑1 (MQ=255)
                                                 ccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCAAAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcc                                                                                                      >  2:495927/1‑150 (MQ=255)
                                                  cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                     <  1:109868/150‑1 (MQ=255)
                                                  cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                     <  1:1231835/150‑1 (MQ=255)
                                                  cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                     <  1:525740/150‑1 (MQ=255)
                                                    gCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgc                                                                                                   >  1:1645842/1‑150 (MQ=255)
                                                     cGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgcc                                                                                                  <  2:1293410/150‑1 (MQ=255)
                                                       gCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAg                                                                                                >  2:216998/1‑150 (MQ=255)
                                                          aCCATCTGGAACAGCTTAGCCGGATGTCCAATCTCCTGCACAATATTCGCATGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCCACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGt                                                                                             <  2:470118/150‑1 (MQ=255)
                                                           ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                            >  2:749787/1‑150 (MQ=255)
                                                           ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                            >  2:604935/1‑150 (MQ=255)
                                                            cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                           <  1:1006600/150‑1 (MQ=255)
                                                            cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                           <  1:1346843/150‑1 (MQ=255)
                                                             aTCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACa                                                                                          <  1:1122635/150‑1 (MQ=255)
                                                                gggAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATaa                                                                                       <  1:1009359/150‑1 (MQ=255)
                                                                  gAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATc                                                                                     >  2:1321290/1‑150 (MQ=255)
                                                                     cAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTc                                                                                  <  1:599577/150‑1 (MQ=255)
                                                                      aGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTcc                                                                                 <  1:862165/150‑1 (MQ=255)
                                                                                 aTGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAgc                                                                      <  1:1246360/150‑1 (MQ=255)
                                                                                    tCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCt                                                                   >  1:706520/1‑150 (MQ=255)
                                                                                     ccAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTa                                                                  >  1:651769/1‑150 (MQ=255)
                                                                                         tctcCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTGAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCGTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATcgc                                                              <  2:1155193/150‑1 (MQ=255)
                                                                                            ccTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGAt                                                           <  1:573309/150‑1 (MQ=255)
                                                                                              tGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATgc                                                         >  2:966116/1‑149 (MQ=255)
                                                                                               gCACAATATTCGCCTGAATTCCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCAGCCGCGTAATCCTGCAACTCCAATGTCGCTCCCTGTAATGCCGGCCAGGGGAGATTAGCTGACCCGGCCAAGGCGGTATCCCGATGGc                                                        >  2:940955/1‑150 (MQ=255)
                                                                                                 acaATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCaa                                                      <  2:1143354/150‑1 (MQ=255)
                                                                                                    atatTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGc                                                   >  1:1448188/1‑150 (MQ=255)
                                                                                                      atTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCag                                                 <  1:604935/150‑1 (MQ=255)
                                                                                                          gCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCagaaag                                             >  2:1492103/1‑150 (MQ=255)
                                                                                                             tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCCCGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                          >  1:1055322/1‑150 (MQ=255)
                                                                                                             tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                          <  1:1507251/150‑1 (MQ=255)
                                                                                                              gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTACTGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                                         >  1:140395/1‑150 (MQ=255)
                                                                                                              gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGATCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                                         >  2:85379/1‑150 (MQ=255)
                                                                                                              gAATAACATTGCGAGACAGCTCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCATGCAACACCAATTTCGCTCCCTGTAATGCCTGACAGGGTACATAATCTTCCACGTCCAAAGCGCTATCGCGATGGCAAATCAGaaagaaag                                         >  2:1565696/1‑150 (MQ=255)
                                                                                                                aTACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGc                                       <  1:978459/150‑1 (MQ=255)
                                                                                                                   ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                    >  2:303458/1‑150 (MQ=255)
                                                                                                                   ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                    >  2:676034/1‑150 (MQ=255)
                                                                                                                   ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                    >  2:1539357/1‑150 (MQ=255)
                                                                                                                   ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                    <  1:92226/150‑1 (MQ=255)
                                                                                                                    cATTGCGCGCCAGCGCTGCGGCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCgg                                   <  1:1400305/150‑1 (MQ=255)
                                                                                                                     aTTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGa                                  >  1:62131/1‑150 (MQ=255)
                                                                                                                           cgcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAAt                            >  1:1253385/1‑150 (MQ=255)
                                                                                                                            gcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATc                           <  2:1600212/150‑1 (MQ=255)
                                                                                                                             ccAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCg                          >  2:1600875/1‑150 (MQ=255)
                                                                                                                               aGCGCTGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt                        <  2:1259251/150‑1 (MQ=255)
                                                                                                                               aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCACCACCAATTTCGCTCGCTGTAATGCCTACCAGGGTACATAATCTTCCACGGCAAAAGCGCTATCGCGATGGCAAACCAAAAAGAAAGGATACGAAAGaaactct                        >  2:93807/1‑145 (MQ=255)
                                                                                                                               aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGATAGGCTCGGAAAGAATCGCt                        <  1:155147/150‑1 (MQ=255)
                                                                                                                               aGCGCGGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt                        <  2:864583/150‑1 (MQ=255)
                                                                                                                                   acgCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcac                    >  2:1642149/3‑150 (MQ=255)
                                                                                                                                    tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTTTTATGCATGCGTGAGTACATAATCTTCAACTGACAAAGAGCTATCTCGATGGCAAAGCTGAAAGAATGGCTCGGAATGAATCGGTTcaca                   >  2:1148810/1‑150 (MQ=255)
                                                                                                                                    tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAACGGCTCGGAAATAATCGCTTcaca                   >  2:1589904/1‑150 (MQ=255)
                                                                                                                                    tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca                   <  2:461522/150‑1 (MQ=255)
                                                                                                                                    tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca                   >  2:1025216/1‑150 (MQ=255)
                                                                                                                                     gCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGGGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAt                  >  2:279429/1‑150 (MQ=255)
                                                                                                                                      cGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt                 >  1:372793/1‑150 (MQ=255)
                                                                                                                                      cGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt                 >  2:1519648/1‑150 (MQ=255)
                                                                                                                                       gTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTg                >  2:218915/1‑150 (MQ=255)
                                                                                                                                        tCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCTCTCCCTGTAATGCCTTCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATTGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGc               <  2:668704/150‑1 (MQ=255)
                                                                                                                                         cAATCAGCGGTCGGCTGCCTGACGCGTAGTCCTGCCAGCCCAGTTTCGCTGCCTGTAATGCCTGCCAGGGTGCATACTCTGCCACGGCCAAAGCGCTATCGCGAGGGCAAAGCAGAAAGAAAGGCTCGGAAAGCATCGCTTCACATTGcc              >  2:979273/1‑149 (MQ=255)
                                                                                                                                         cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa              >  1:722391/1‑150 (MQ=255)
                                                                                                                                         cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAGTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTACCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa              <  1:1040305/150‑1 (MQ=255)
                                                                                                                                           aTCAGCGGTCCGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACAAAATCTTCCACGGCCAAAGCGCTATCGCGAAGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCATCACATTGCAGa            >  1:228165/1‑150 (MQ=255)
                                                                                                                                             cAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATc          <  1:1217936/150‑1 (MQ=255)
                                                                                                                                             cAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATc          <  2:1478138/150‑1 (MQ=255)
                                                                                                                                              aGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCg         >  1:1573936/1‑150 (MQ=255)
                                                                                                                                               gCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGc        >  2:254484/1‑150 (MQ=255)
                                                                                                                                                cGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGcc       <  2:1085445/150‑1 (MQ=255)
                                                                                                                                                     ggCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCTATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTg  >  2:845110/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCTTCAGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTG  >  NC_000913/2526101‑2526399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: