Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,526,255 C→A 6.4% G201V (GGC→GTC) ‡ yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,526,2550CA 6.4% 276.7 / 7.0 94G201V (GGC→GTC) ‡yfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (42/46);  new base A (3/3);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

AGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTGGGCC  >  NC_000913/2526107‑2526403
                                                                                                                                                    |                                                                                                                                                    
aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                                     >  2:228561/1‑150 (MQ=255)
aGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGcc                                                                                                                                                     >  2:1217936/1‑150 (MQ=255)
 ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCCGCGCTGCGTCAATCAGCGGTCGGCGGccg                                                                                                                                                    >  1:1478138/1‑149 (MQ=255)
 ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                                    <  1:438154/150‑1 (MQ=255)
 ggCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCt                                                                                                                                                    >  1:1293410/1‑150 (MQ=255)
    aaTGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAc                                                                                                                                                 <  1:1108192/150‑1 (MQ=255)
        gTAGCGCCAGTGCGGGAAGAATACTGATGCCGCTGCCTGCGGCTACCAGCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGACTACCATTGCGCGCCAGCGCTGCGGCAGGCAGCCGACCGCTGATTGAcgcag                                                                                                                                             <  1:1506656/150‑3 (MQ=255)
         tAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAGGCAGCCGACCGCTGATTGAcgcagc                                                                                                                                            <  1:1660870/150‑4 (MQ=255)
           gcgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAAt                                                                                                                                          <  2:118099/150‑1 (MQ=255)
            cgcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATc                                                                                                                                         >  1:1414106/1‑150 (MQ=255)
             gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                                        <  2:1243763/150‑1 (MQ=255)
             gcCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATcc                                                                                                                                        <  2:1243765/150‑1 (MQ=255)
                   gCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAac                                                                                                                                  <  1:603126/150‑1 (MQ=255)
                     gggAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGGCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAacac                                                                                                                                <  1:312482/150‑1 (MQ=255)
                        aagaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCaa                                                                                                                             >  2:1507251/1‑150 (MQ=255)
                          gaaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAAGCTCCTGCCCAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAtt                                                                                                                           >  2:302323/1‑150 (MQ=255)
                           aaTACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGACGGATGTCCAAACTCCTGCACAATATTCGCATGAATACCATTGAGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAttt                                                                                                                          >  1:668704/1‑150 (MQ=255)
                             tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                                        <  2:149769/150‑1 (MQ=255)
                             tACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCg                                                                                                                        <  2:1205243/150‑1 (MQ=255)
                              aCTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTTAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGc                                                                                                                       <  2:1397517/150‑1 (MQ=255)
                               cTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCt                                                                                                                      >  1:461522/1‑150 (MQ=255)
                                    gccgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTg                                                                                                                 <  1:1731234/150‑1 (MQ=255)
                                      cgatgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTa                                                                                                               >  2:1400305/1‑150 (MQ=255)
                                        ctgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCTGCTGCCTGACGCGTAAGCCAGCAACACCAATTTCGCTCCCTGTAAt                                                                                                             <  1:266127/149‑1 (MQ=255)
                                        atgccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAt                                                                                                             <  1:852093/150‑1 (MQ=255)
                                           ccTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCAAAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcc                                                                                                          >  2:495927/1‑150 (MQ=255)
                                            cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                         <  1:109868/150‑1 (MQ=255)
                                            cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                         <  1:1231835/150‑1 (MQ=255)
                                            cTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcct                                                                                                         <  1:525740/150‑1 (MQ=255)
                                              gCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgc                                                                                                       >  1:1645842/1‑150 (MQ=255)
                                               cGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAAtgcctgcc                                                                                                      <  2:1293410/150‑1 (MQ=255)
                                                 gCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAg                                                                                                    >  2:216998/1‑150 (MQ=255)
                                                    aCCATCTGGAACAGCTTAGCCGGATGTCCAATCTCCTGCACAATATTCGCATGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCCACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGt                                                                                                 <  2:470118/150‑1 (MQ=255)
                                                     ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                                >  2:604935/1‑150 (MQ=255)
                                                     ccATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTa                                                                                                >  2:749787/1‑150 (MQ=255)
                                                      cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                               <  1:1346843/150‑1 (MQ=255)
                                                      cATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTAc                                                                                               <  1:1006600/150‑1 (MQ=255)
                                                       aTCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACa                                                                                              <  1:1122635/150‑1 (MQ=255)
                                                          gggAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATaa                                                                                           <  1:1009359/150‑1 (MQ=255)
                                                            gAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATc                                                                                         >  2:1321290/1‑150 (MQ=255)
                                                               cAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTc                                                                                      <  1:599577/150‑1 (MQ=255)
                                                                aGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTcc                                                                                     <  1:862165/150‑1 (MQ=255)
                                                                           aTGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAgc                                                                          <  1:1246360/150‑1 (MQ=255)
                                                                              tCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCt                                                                       >  1:706520/1‑150 (MQ=255)
                                                                               ccAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTa                                                                      >  1:651769/1‑150 (MQ=255)
                                                                                   tctcCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTGAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCGTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATcgc                                                                  <  2:1155193/150‑1 (MQ=255)
                                                                                      ccTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGAt                                                               <  1:573309/150‑1 (MQ=255)
                                                                                        tGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATgc                                                             >  2:966116/1‑149 (MQ=255)
                                                                                         gCACAATATTCGCCTGAATTCCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCAGCCGCGTAATCCTGCAACTCCAATGTCGCTCCCTGTAATGCCGGCCAGGGGAGATTAGCTGACCCGGCCAAGGCGGTATCCCGATGGc                                                            >  2:940955/1‑150 (MQ=255)
                                                                                           acaATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCaa                                                          <  2:1143354/150‑1 (MQ=255)
                                                                                              atatTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGc                                                       >  1:1448188/1‑150 (MQ=255)
                                                                                                atTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCag                                                     <  1:604935/150‑1 (MQ=255)
                                                                                                    gCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCagaaag                                                 >  2:1492103/1‑150 (MQ=255)
                                                                                                       tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCCCGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                              >  1:1055322/1‑150 (MQ=255)
                                                                                                       tGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAgaaagaaa                                              <  1:1507251/150‑1 (MQ=255)
                                                                                                        gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTACTGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                                             >  1:140395/1‑150 (MQ=255)
                                                                                                        gAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGATCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGaaagaaag                                             >  2:85379/1‑150 (MQ=255)
                                                                                                        gAATAACATTGCGAGACAGCTCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCATGCAACACCAATTTCGCTCCCTGTAATGCCTGACAGGGTACATAATCTTCCACGTCCAAAGCGCTATCGCGATGGCAAATCAGaaagaaag                                             >  2:1565696/1‑150 (MQ=255)
                                                                                                          aTACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGc                                           <  1:978459/150‑1 (MQ=255)
                                                                                                             ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                        >  2:303458/1‑150 (MQ=255)
                                                                                                             ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                        >  2:676034/1‑150 (MQ=255)
                                                                                                             ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                        >  2:1539357/1‑150 (MQ=255)
                                                                                                             ccATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCg                                        <  1:92226/150‑1 (MQ=255)
                                                                                                              cATTGCGCGCCAGCGCTGCGGCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCgg                                       <  1:1400305/150‑1 (MQ=255)
                                                                                                               aTTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGa                                      >  1:62131/1‑150 (MQ=255)
                                                                                                                     cgcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAAt                                >  1:1253385/1‑150 (MQ=255)
                                                                                                                      gcCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATc                               <  2:1600212/150‑1 (MQ=255)
                                                                                                                       ccAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCg                              >  2:1600875/1‑150 (MQ=255)
                                                                                                                         aGCGCTGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt                            <  2:1259251/150‑1 (MQ=255)
                                                                                                                         aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCACCACCAATTTCGCTCGCTGTAATGCCTACCAGGGTACATAATCTTCCACGGCAAAAGCGCTATCGCGATGGCAAACCAAAAAGAAAGGATACGAAAGaaactct                            >  2:93807/1‑145 (MQ=255)
                                                                                                                         aGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGATAGGCTCGGAAAGAATCGCt                            <  1:155147/150‑1 (MQ=255)
                                                                                                                         aGCGCGGCGTCAATCAGCTGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAACGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCt                            <  2:864583/150‑1 (MQ=255)
                                                                                                                             acgCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcac                        >  2:1642149/3‑150 (MQ=255)
                                                                                                                              tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTTTTATGCATGCGTGAGTACATAATCTTCAACTGACAAAGAGCTATCTCGATGGCAAAGCTGAAAGAATGGCTCGGAATGAATCGGTTcaca                       >  2:1148810/1‑150 (MQ=255)
                                                                                                                              tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAACGGCTCGGAAATAATCGCTTcaca                       >  2:1589904/1‑150 (MQ=255)
                                                                                                                              tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca                       <  2:461522/150‑1 (MQ=255)
                                                                                                                              tGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTcaca                       >  2:1025216/1‑150 (MQ=255)
                                                                                                                               gCGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGGGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAt                      >  2:279429/1‑150 (MQ=255)
                                                                                                                                cGTCAGGCAGCCGACCGCTGATTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt                     >  1:372793/1‑150 (MQ=255)
                                                                                                                                cGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACAtt                     >  2:1519648/1‑150 (MQ=255)
                                                                                                                                 gTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTg                    >  2:218915/1‑150 (MQ=255)
                                                                                                                                  tCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCTCTCCCTGTAATGCCTTCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATTGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGc                   <  2:668704/150‑1 (MQ=255)
                                                                                                                                   cAATCAGCGGTCGGCTGCCTGACGCGTAGTCCTGCCAGCCCAGTTTCGCTGCCTGTAATGCCTGCCAGGGTGCATACTCTGCCACGGCCAAAGCGCTATCGCGAGGGCAAAGCAGAAAGAAAGGCTCGGAAAGCATCGCTTCACATTGcc                  >  2:979273/1‑149 (MQ=255)
                                                                                                                                   cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa                  >  1:722391/1‑150 (MQ=255)
                                                                                                                                   cAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAAGTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTACCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCa                  <  1:1040305/150‑1 (MQ=255)
                                                                                                                                     aTCAGCGGTCCGCTGCCTGACGCGTAATCCTGCATCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACAAAATCTTCCACGGCCAAAGCGCTATCGCGAAGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCATCACATTGCAGa                >  1:228165/1‑150 (MQ=255)
                                                                                                                                       cAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATc              <  2:1478138/150‑1 (MQ=255)
                                                                                                                                       cAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATc              <  1:1217936/150‑1 (MQ=255)
                                                                                                                                        aGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCg             >  1:1573936/1‑150 (MQ=255)
                                                                                                                                         gCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGc            >  2:254484/1‑150 (MQ=255)
                                                                                                                                          cGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGcc           <  2:1085445/150‑1 (MQ=255)
                                                                                                                                               ggCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCTATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTg      >  2:845110/1‑150 (MQ=255)
                                                                                                                                                 cTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTggg    >  2:731890/1‑150 (MQ=255)
                                                                                                                                                 cTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTggg    <  2:1301295/150‑1 (MQ=255)
                                                                                                                                                   gCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCCCGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTGGGcc  <  1:967661/150‑1 (MQ=255)
                                                                                                                                                   gCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCAGTGGGcg  <  1:454645/150‑2 (MQ=255)
                                                                                                                                                   gCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTGGGcc  <  1:1600875/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGCAATGGTAGCGCCAGTGCGGGAAGAATACTGATGCCGATGCCTGCGGCTACCATCGGGAACAGCGTCGCCGGATGTCCAATCTCCTGCACAATATTCGCCTGAATACCATTGCGCGCCAGCGCTGCGTCAATCAGCGGTCGGCTGCCTGACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTGGGCC  >  NC_000913/2526107‑2526403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: