Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,533,416 T→A 6.6% intergenic (+36/‑348) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,533,4160TA 6.6% 310.0 / 8.9 106intergenic (+36/‑348)cysK/ptsHcysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
Reads supporting (aligned to +/- strand):  ref base T (43/56);  new base A (3/4);  total (46/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 110 total aligned reads displayed.

ATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTT  >  NC_000913/2533268‑2533565
                                                                                                                                                    |                                                                                                                                                     
aTGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCtttt                                                                                                                                                      >  2:1659738/1‑150 (MQ=255)
 tGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCttttt                                                                                                                                                     >  1:1243137/1‑150 (MQ=255)
 tGAAAGCTATACCAACAAGAATATTCTGGTTATACTCCCATCATCGGGAGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGGGTGTTAAAAATGCGTAAAAAAGCACGtttta                                                                                                                                                     >  1:464070/1‑149 (MQ=255)
   aaaGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAg                                                                                                                                                   <  2:1575149/150‑1 (MQ=255)
    aaGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAgg                                                                                                                                                  <  1:465238/150‑1 (MQ=255)
      tcTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTg                                                                                                                                                <  1:746428/149‑1 (MQ=255)
         ttACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCtt                                                                                                                                             <  2:614399/150‑1 (MQ=255)
         ttACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACATTTTTAGGTGCtt                                                                                                                                             >  1:1146334/1‑150 (MQ=255)
          tACCCACAAGAAAATAGGGGTTACTCTACCCTCCTCGGGCGATCGTTATTTAAGCTCCGCATTGTTTTCAGATATATACACTGAGAAAGATTTGCTACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCttt                                                                                                                                            <  2:345958/150‑1 (MQ=255)
          tACCAACAAGAATATTGTGGTTATTCTACCATCATCGTGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTAAAAAGGTGCttt                                                                                                                                            <  2:1350604/150‑1 (MQ=255)
          tACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCttt                                                                                                                                            <  1:736767/150‑1 (MQ=255)
            ccAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCttttt                                                                                                                                          >  2:1715923/1‑150 (MQ=255)
            ccAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCttttt                                                                                                                                          <  2:781109/150‑1 (MQ=255)
              aacaaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTtg                                                                                                                                        <  2:1697163/150‑1 (MQ=255)
               acaaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTAAAAAGGTGCttttttac                                                                                                                                       <  1:115597/150‑3 (MQ=255)
               acaaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACAGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTAAAAAGGTGCttttttac                                                                                                                                       <  2:70513/150‑3 (MQ=255)
                 aaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTgg                                                                                                                                     <  1:122488/150‑1 (MQ=255)
                    aaTATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCt                                                                                                                                  <  2:373680/150‑1 (MQ=255)
                       atTGTGGTTATTCTACCATCCTCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCt                                                                                                                               <  1:1555560/150‑1 (MQ=255)
                       atTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCATTTTAGGTGCTTTTTTGTGGCCTGCt                                                                                                                               >  2:620264/1‑150 (MQ=255)
                        ttGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCtt                                                                                                                              <  1:1308384/150‑1 (MQ=255)
                        ttGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCtt                                                                                                                              >  2:1475279/1‑150 (MQ=255)
                           tgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCaa                                                                                                                           >  1:737148/1‑150 (MQ=255)
                            ggTTATTCTACCATCTTCGGGTGAGCGTTAGTTAAGCACCGCTTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTACTGCCAGCTTGTTAAAAATGAGTAAAAAATCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCaaa                                                                                                                          <  2:1227281/150‑1 (MQ=255)
                            ggTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCaaa                                                                                                                          >  1:1292909/1‑150 (MQ=255)
                                    tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAACAGGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                                                                                  <  2:1223866/150‑1 (MQ=255)
                                    tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                                                                                  <  2:581369/150‑1 (MQ=255)
                                      ccATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcccc                                                                                                                >  1:1558305/1‑150 (MQ=255)
                                        atcatcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCAGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCAcctc                                                                                                              >  1:174307/1‑150 (MQ=255)
                                             cGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGc                                                                                                         >  1:1190268/1‑150 (MQ=255)
                                                 tGATCGGTATTTAAGCACAGTATTTTTTGCCGATCTCTTCTCTGAGAAAGAATTGCCACATTAATGGCAGCTTGTTAATAATTCGTAAAAAAGCTCCTTTTTAGGTGCTTTTTTGTGGCCTGCTTGAAACTTTCGCCCCTCCTGGCATTg                                                                                                     <  2:852591/150‑1 (MQ=255)
                                                 tGAGGGGTATTTAACCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGATTTGCTACAGTAATGCCAGCTTGTTAAAAATGCGTATAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTg                                                                                                     <  2:326950/150‑1 (MQ=255)
                                                 tGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTg                                                                                                     <  2:852588/150‑1 (MQ=255)
                                                 tGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTg                                                                                                     <  1:1443221/150‑1 (MQ=255)
                                                  gAGCGTTATTTAAGCCCCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTATTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGa                                                                                                    >  1:1043769/1‑150 (MQ=255)
                                                   aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGAt                                                                                                   >  1:246501/1‑150 (MQ=255)
                                                     cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGGCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTc                                                                                                 <  2:1328639/150‑1 (MQ=255)
                                                      gTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCa                                                                                                >  2:692626/1‑150 (MQ=255)
                                                       ttATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                                                                                               <  1:737169/150‑1 (MQ=255)
                                                       ttATTTAAGCACCACATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                                                                                               <  1:813236/150‑1 (MQ=255)
                                                        tATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                                                                                              <  1:1729144/150‑1 (MQ=255)
                                                         aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                             <  1:478061/150‑1 (MQ=255)
                                                         aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                             <  2:1243137/150‑1 (MQ=255)
                                                         aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                             <  1:1475279/150‑1 (MQ=255)
                                                         aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTGAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                             >  2:844345/1‑150 (MQ=255)
                                                         aTTTAAGCAACGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCATCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                             <  2:868221/150‑1 (MQ=255)
                                                           ttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTg                                                                                           <  2:482024/150‑1 (MQ=255)
                                                             aaGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTATGTGATATTTTGTGGCATGCTTCAAACTTTCGCCCCTCCTGGAATTGATTCAGCCTGTc                                                                                         >  1:1462511/1‑150 (MQ=255)
                                                                 aCCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGaa                                                                                     >  2:1407648/1‑150 (MQ=255)
                                                                 aCCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGaa                                                                                     >  1:754466/1‑150 (MQ=255)
                                                                  ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                                                                                    <  1:615907/150‑1 (MQ=255)
                                                                     cATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                                                                                 >  2:488023/1‑150 (MQ=255)
                                                                     cATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                                                                                 <  1:1186848/150‑1 (MQ=255)
                                                                     cATTGTTTGACGATCTCATCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAATAATGCGTAAAAAAGGACCTTTTTAGGTGCTTTTTTGTGTCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                                                                                 <  1:277452/150‑1 (MQ=255)
                                                                      aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGt                                                                                <  1:779515/150‑1 (MQ=255)
                                                                        tGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAt                                                                              <  1:484014/150‑1 (MQ=255)
                                                                         gTTTGCCGATCTCTTCACTGAGAAATAATTGCAACAGTAATGCGAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAtt                                                                             <  1:326432/150‑1 (MQ=255)
                                                                              ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAcc                                                                        >  2:1528564/1‑150 (MQ=255)
                                                                              ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAcc                                                                        <  2:260413/150‑1 (MQ=255)
                                                                                gATCTCTTCACTGAGAAAGAATTGCAACATTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg                                                                      <  1:928718/150‑1 (MQ=255)
                                                                                gATCTCTTCACTGAGAAAGAATTGCAACAGTCATGCCATCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg                                                                      <  2:1323641/150‑1 (MQ=255)
                                                                                     ctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa                                                                 >  1:1549498/1‑150 (MQ=255)
                                                                                     ctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCGGGCGGAACTGGTATTTAACCAGACTaa                                                                 >  2:1714613/1‑150 (MQ=255)
                                                                                      ttCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAat                                                                >  1:1717963/1‑150 (MQ=255)
                                                                                       tCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCACCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAatt                                                               >  2:832753/1‑150 (MQ=255)
                                                                                           tGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAttt                                                           <  2:286227/150‑1 (MQ=255)
                                                                                                aagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATg                                                      <  1:1410362/150‑1 (MQ=255)
                                                                                                    aTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGcgcg                                                  >  2:373715/1‑150 (MQ=255)
                                                                                                      tGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaa                                                >  1:1080293/1‑150 (MQ=255)
                                                                                                       gCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                               >  1:992123/1‑150 (MQ=255)
                                                                                                        cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCGTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGGATTTAACCAGACTAATTAGTTTGATGCGCGAAAt                                              >  2:132195/1‑150 (MQ=255)
                                                                                                          aCAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTa                                            >  1:391443/1‑150 (MQ=255)
                                                                                                             ttAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGATTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTTGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATc                                         <  1:1636182/149‑1 (MQ=255)
                                                                                                             gTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATc                                         >  2:932917/1‑150 (MQ=255)
                                                                                                             gTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATc                                         >  1:1715351/1‑150 (MQ=255)
                                                                                                              tAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTAAACCAGACTAATTATTTTGATGCGCGAAATTAATCg                                        >  2:915608/1‑150 (MQ=255)
                                                                                                                  gCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTAc                                    >  2:540754/1‑150 (MQ=255)
                                                                                                                    cAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAg                                  <  1:160999/150‑1 (MQ=255)
                                                                                                                            tAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGTGCGAAATTAATCGTTACAGGAAAAGcc                          <  1:1414238/150‑1 (MQ=255)
                                                                                                                            tAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGcc                          <  1:488023/150‑1 (MQ=255)
                                                                                                                             aaaaaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTAGATGCGCGAAATTATTCGTTACAGGAAAAGCCa                         >  2:1351792/1‑150 (MQ=255)
                                                                                                                             aaaaaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGAGTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCa                         >  2:714477/1‑150 (MQ=255)
                                                                                                                               aaaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCaaa                       <  1:546332/150‑1 (MQ=255)
                                                                                                                                      tAAAATAGCACCTTTTTATTTGCTTTTTTGTGGCCTGATTCAAACTTTAGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTTGTATTTAAGCAGAGTAATTATTTTGATGCGCGTTATTTATCGTTACAGGAAAAGCCAAAGCTGAAt                <  1:1641285/150‑1 (MQ=255)
                                                                                                                                      tAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt                <  1:1280778/150‑1 (MQ=255)
                                                                                                                                      tAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt                >  2:938091/1‑150 (MQ=255)
                                                                                                                                        aaaaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCa                         <  1:1536066/139‑1 (MQ=255)
                                                                                                                                        aaaaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCa                         >  2:1536066/1‑139 (MQ=255)
                                                                                                                                         aaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa             >  1:1471495/1‑150 (MQ=255)
                                                                                                                                         aaaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa             >  1:452018/1‑150 (MQ=255)
                                                                                                                                          aaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAt            >  2:20766/1‑150 (MQ=255)
                                                                                                                                          aaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAGCCAAAGCTGAATCGAtt           >  2:782924/1‑150 (MQ=255)
                                                                                                                                            aGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAttt          <  1:1487177/150‑1 (MQ=255)
                                                                                                                                             gCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTATAGGAAAAGCCAAAGCTGAATCGAtttt         >  2:1683156/1‑150 (MQ=255)
                                                                                                                                             gCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtttt         <  2:515977/150‑1 (MQ=255)
                                                                                                                                             gCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtttt         >  2:866198/1‑150 (MQ=255)
                                                                                                                                             gCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtttt         <  2:895028/150‑1 (MQ=255)
                                                                                                                                                ccTTTTTAGGTGCTTTTTTGTGGCCTGCTGCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg      <  2:381225/150‑1 (MQ=255)
                                                                                                                                                 cTTTTTAGGTTCTTTTTTGTTGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGa     <  1:833719/150‑1 (MQ=255)
                                                                                                                                                    tttAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAttt  <  1:1506533/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTT  >  NC_000913/2533268‑2533565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: