Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,568,253 G→T 6.9% intergenic (‑146/+71) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,568,2530GT 6.9% 309.1 / 8.0 110intergenic (‑146/+71)eutH/eutGethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (54/47);  new base T (3/6);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTCC  >  NC_000913/2568104‑2568402
                                                                                                                                                     |                                                                                                                                                     
ccATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGTCGTg                                                                                                                                                       >  2:778048/1‑150 (MQ=255)
ccATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg                                                                                                                                                       >  1:1677588/1‑150 (MQ=255)
ccATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg                                                                                                                                                       >  2:411311/1‑150 (MQ=255)
 cATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGa                                                                                                                                                      >  1:1498817/1‑150 (MQ=255)
    cGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACg                                                                                                                                                   >  2:1395215/1‑150 (MQ=255)
     gCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc                                                                                                                                                  >  2:1123664/1‑150 (MQ=255)
      ccTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc                                                                                                                                                 <  2:863230/150‑1 (MQ=255)
       cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt                                                                                                                                                >  2:534581/1‑150 (MQ=255)
        tttACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGCACGCCtt                                                                                                                                               >  2:716758/1‑150 (MQ=255)
        tttACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCtt                                                                                                                                               >  2:63904/1‑150 (MQ=255)
           aCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                                                            <  1:1027171/150‑1 (MQ=255)
           aCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                                                            >  2:1480135/1‑150 (MQ=255)
             tCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                          <  2:1210438/150‑1 (MQ=18)
              cccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc                                                                                                                                         >  2:467958/1‑150 (MQ=255)
               ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACTTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGAAATTGCACCGCGCCACTGGCGTATAAGGCGTTCACGCCGCATCCGcc                                                                                                                                        <  1:1683142/150‑1 (MQ=18)
               ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGcc                                                                                                                                        >  1:1645589/1‑150 (MQ=255)
                cTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                                                                       <  2:1435063/150‑1 (MQ=255)
                 tggtAGTTGATATTTATTGGCGGAGGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATAAGGCGTTCACGCCGCATCCGccag                                                                                                                                      <  1:65312/147‑3 (MQ=18)
                 tGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCATTTGCACCGCGCCACTGTCGGATGCGGCGTGAACGCCTTATCAGCCCt                                                                                                                                      >  1:294608/1‑150 (MQ=255)
                 tGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCt                                                                                                                                      <  2:1063499/150‑1 (MQ=255)
                        tgttATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg                                                                                                                               <  1:557824/150‑1 (MQ=255)
                         gttATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc                                                                                                                              <  2:1617943/150‑1 (MQ=255)
                           tatttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTTCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTTAACCGCGCCACAGGCGAATCCGACTTGAACGACTAATCCGACCTAAATCTGCaa                                                                                                                            >  2:301216/1‑150 (MQ=17)
                            atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCGTATCCGCCCTACATGTGCAAt                                                                                                                           >  1:640492/1‑150 (MQ=255)
                                attGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg                                                                                                                       <  1:410633/150‑1 (MQ=255)
                                   ggCGGATGCGGCGTAAACGCCTTATCCTCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGATCGCCTTATCCGCCCTACATGAGCAATCCCGTAg                                                                                                                    >  2:1297043/1‑150 (MQ=255)
                                   ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGCACGCCTTATCCGCCCTACATGTGCAATCCCGGAg                                                                                                                    >  1:29588/1‑150 (MQ=255)
                                    gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg                                                                                                                   >  1:1477946/1‑150 (MQ=255)
                                     cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc                                                                                                                  >  2:737353/1‑150 (MQ=255)
                                      ggATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt                                                                                                                 >  2:791813/1‑150 (MQ=255)
                                       tattcggcgTAAACGCCTTATCCGCCGTACTTGTGCATGCCCGTAGGCTGGATAAGACGCGTCAAGCGTCGGATCCGGCAATTGCACGGCGCGACTGGCGGATGCGGCTTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg                                                                                                                <  1:211421/146‑1 (MQ=17)
                                       gATGCGGCGTAAACGCCGTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg                                                                                                                <  2:1198967/150‑1 (MQ=255)
                                        aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATACGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg                                                                                                               <  1:1303250/150‑1 (MQ=255)
                                           cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa                                                                                                            <  1:1111145/150‑1 (MQ=255)
                                           cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa                                                                                                            <  2:1001191/150‑1 (MQ=255)
                                             gcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCGTACATGTGCAATCCCCTAGGCTGGAGaac                                                                                                          >  1:123579/1‑149 (MQ=255)
                                              cgTAAACGCCTTATCCGCCCTACATGTACAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa                                                                                                         <  1:482485/150‑1 (MQ=255)
                                                 aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTTGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGc                                                                                                      <  1:988283/150‑1 (MQ=255)
                                                 aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCTTAGGCTGGATAAGaatc                                                                                                      >  2:24959/1‑147 (MQ=255)
                                                 aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGc                                                                                                      <  1:590816/150‑1 (MQ=255)
                                                 aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGc                                                                                                      >  2:416303/1‑150 (MQ=255)
                                                 aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGAACCGCGCCACAGGCCGATGCGGCATGAACGCCGAATCCGCCCTACATGTGCAATCCCGTAGGATCGCTAAGAAGc                                                                                                      >  2:284762/1‑150 (MQ=17)
                                                   aCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGTAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCgg                                                                                                    >  2:667585/1‑150 (MQ=255)
                                                    cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGc                                                                                                   >  2:271270/1‑150 (MQ=255)
                                                    cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGc                                                                                                   >  2:1117511/1‑150 (MQ=255)
                                                     gacgtATCCGCACTACATGTTCAATCCCGTCGGCTGGATCAGACGCCGCAAGCGTCGAATCCGGTTATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCa                                                                                                  <  2:1050057/146‑1 (MQ=18)
                                                     gCCTTATCCGCCCTACATGTGCAATCACGTAGGCTGTATAAGACGCGGCAAGCGTCGCATCCGGCTTTTGGACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCa                                                                                                  <  2:270188/150‑1 (MQ=18)
                                                          aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                                                                             <  1:886068/150‑1 (MQ=255)
                                                            ccGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACAGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCATTCCCGTAGGCTTGATAAGATGCGGCAAGCGTCg                                                                                           <  1:1555289/150‑1 (MQ=255)
                                                             cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACGGGCGGCTGCGGCGTGCACGCCTTATCAGCCCTACATGTGCAATCCCGTAGGCTAGATAACATGCGGCACGCCTCGc                                                                                          >  2:1124124/1‑150 (MQ=18)
                                                                  tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACAGGAGGATGCGGCGTGAACGACTTATCAACCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCTTAGCATCCg                                                                                     >  2:270931/1‑150 (MQ=37)
                                                                   aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa                                                                                         >  2:780487/1‑145 (MQ=255)
                                                                   aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa                                                                                         <  1:780487/145‑1 (MQ=255)
                                                                   aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg                                                                                    >  2:1021265/1‑150 (MQ=255)
                                                                      tgtgCATTCACGTATGCTGGATAAGACTCGGCAAGCGTCGCATCCGGCAATTGCACCGATCAACTGGCGGATGCGGCGTTAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt                                                                                 <  2:212203/150‑1 (MQ=18)
                                                                      tgtgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGACCGCCTTATCCGCCCTTCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt                                                                                 >  1:1177840/1‑150 (MQ=255)
                                                                        tgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAttt                                                                               <  2:1677588/150‑1 (MQ=255)
                                                                          cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAt                                                                             <  1:1680038/150‑1 (MQ=255)
                                                                          cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCCTAGGCTGGATAACATGCGCCAATCGTCGCATCCGGCATTTAt                                                                             >  2:297956/1‑150 (MQ=255)
                                                                              cccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcg                                                                         >  1:1673516/1‑150 (MQ=255)
                                                                               ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcgc                                                                        <  1:667965/150‑1 (MQ=255)
                                                                               ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcgc                                                                        >  2:832050/1‑150 (MQ=255)
                                                                                        ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTa                                                               >  1:910384/1‑150 (MQ=255)
                                                                                           tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATGTATTGCGCCGCTGCGTACAg                                                            >  1:63582/1‑150 (MQ=255)
                                                                                             aGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGTATAAGATGCGGCAAGCGTCGCATCCGGCATGTATTGCGCCGCTGCGTCCAGGc                                                          >  1:312707/1‑150 (MQ=255)
                                                                                              gACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGcc                                                         >  2:868546/1‑150 (MQ=255)
                                                                                                      aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt                                                 <  2:1473129/150‑1 (MQ=255)
                                                                                                      aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt                                                 <  1:737353/150‑1 (MQ=255)
                                                                                                      aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt                                                 >  2:1254089/1‑150 (MQ=255)
                                                                                                        gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGc                                               <  1:1395215/150‑1 (MQ=255)
                                                                                                           tCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTcc                                            <  2:445622/150‑1 (MQ=255)
                                                                                                            cGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCa                                           >  2:913230/1‑150 (MQ=255)
                                                                                                             gCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAg                                          >  2:494552/1‑150 (MQ=255)
                                                                                                               aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTTAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGc                                        <  2:1145280/150‑1 (MQ=255)
                                                                                                                 ccGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTg                                      <  1:1001683/150‑1 (MQ=255)
                                                                                                                  cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTgg                                     >  2:919151/1‑150 (MQ=255)
                                                                                                                  cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTgg                                     >  2:862014/1‑150 (MQ=255)
                                                                                                                  cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTgg                                     >  2:83410/1‑150 (MQ=255)
                                                                                                                   ggCAATTGCACCGCGCCACTGGCGGATGCGGCGTTAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGc                                    <  2:294608/150‑1 (MQ=255)
                                                                                                                   ggCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGc                                    >  1:1489195/1‑150 (MQ=255)
                                                                                                                    gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCg                                   >  1:352381/1‑150 (MQ=255)
                                                                                                                     cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCgg                                  >  2:80393/1‑150 (MQ=255)
                                                                                                                        ttGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTAc                               <  1:791813/150‑1 (MQ=255)
                                                                                                                           cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTAcgcg                            <  1:1123664/150‑1 (MQ=255)
                                                                                                                                 gcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTAc                      <  2:411894/150‑1 (MQ=255)
                                                                                                                                     cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcg                  >  2:1591233/1‑150 (MQ=255)
                                                                                                                                     agggCGGATAAGGCGTTCACGCCGCATCCGCCCGACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGCCAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcg                  >  2:708048/3‑150 (MQ=25)
                                                                                                                                      tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTCGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcgc                 <  1:284762/150‑1 (MQ=255)
                                                                                                                                      tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcgc                 <  1:680296/150‑1 (MQ=255)
                                                                                                                                       ggCGGATAAGGCGTTCACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCa                >  1:1125962/1‑150 (MQ=37)
                                                                                                                                        gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAg               <  1:726784/150‑1 (MQ=255)
                                                                                                                                        gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAg               <  1:919151/150‑1 (MQ=255)
                                                                                                                                        gCGGATGCGGCGTGAACGCCGTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAg               <  1:63904/150‑1 (MQ=255)
                                                                                                                                          ggataaggcgTTCACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGAc             >  2:455251/7‑150 (MQ=37)
                                                                                                                                          ggATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGAc             <  1:1309454/150‑1 (MQ=255)
                                                                                                                                             tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATATGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGCCaaa          >  1:53185/1‑150 (MQ=255)
                                                                                                                                              gcggcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAat         >  1:1357039/1‑150 (MQ=255)
                                                                                                                                              gcggcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAat         <  2:901783/150‑1 (MQ=255)
                                                                                                                                                 gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATc      <  1:467958/150‑1 (MQ=255)
                                                                                                                                                     gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTcc  <  2:82387/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTCC  >  NC_000913/2568104‑2568402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: