Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,568,260 T→G 7.7% intergenic (‑153/+64) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,568,2600TG 7.7% 268.9 / 9.7 107intergenic (‑153/+64)eutH/eutGethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (48/49);  new base G (5/4);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 111 total aligned reads displayed.

CTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTCCAGCGC  >  NC_000913/2568111‑2568407
                                                                                                                                                     |                                                                                                                                                   
cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt                                                                                                                                                     >  2:534581/1‑150 (MQ=255)
cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt                                                                                                                                                     <  2:768812/150‑1 (MQ=255)
 tttACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGCACGCCtt                                                                                                                                                    >  2:716758/1‑150 (MQ=255)
 tttACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCtt                                                                                                                                                    >  2:63904/1‑150 (MQ=255)
    aCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                 <  1:1027171/150‑1 (MQ=255)
    aCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                 >  2:1480135/1‑150 (MQ=255)
      tCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                               <  2:1210438/150‑1 (MQ=18)
       cccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc                                                                                                                                              >  2:467958/1‑150 (MQ=255)
        ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACTTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGAAATTGCACCGCGCCACTGGCGTATAAGGCGTTCACGCCGCATCCGcc                                                                                                                                             <  1:1683142/150‑1 (MQ=18)
        ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGcc                                                                                                                                             >  1:1645589/1‑150 (MQ=255)
         cTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                                                                            <  2:1435063/150‑1 (MQ=255)
          tggtAGTTGATATTTATTGGCGGAGGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATAAGGCGTTCACGCCGCATCCGccag                                                                                                                                           <  1:65312/147‑3 (MQ=18)
          tGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCATTTGCACCGCGCCACTGTCGGATGCGGCGTGAACGCCTTATCAGCCCt                                                                                                                                           >  1:294608/1‑150 (MQ=255)
          tGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCt                                                                                                                                           <  2:1063499/150‑1 (MQ=255)
                 tgttATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg                                                                                                                                    <  1:557824/150‑1 (MQ=255)
                  gttATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc                                                                                                                                   <  2:1617943/150‑1 (MQ=255)
                   ttatttatTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCa                                                                                                                                  <  2:482910/150‑1 (MQ=255)
                    tatttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTTCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTTAACCGCGCCACAGGCGAATCCGACTTGAACGACTAATCCGACCTAAATCTGCaa                                                                                                                                 >  2:301216/1‑150 (MQ=17)
                     atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCGTATCCGCCCTACATGTGCAAt                                                                                                                                >  1:640492/1‑150 (MQ=255)
                         attGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg                                                                                                                            <  1:410633/150‑1 (MQ=255)
                          agggCGGATAAGGCGTTTACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGAGCAATCCCgg                                                                                                                           >  2:262570/3‑149 (MQ=17)
                            ggCGGATGCGGCGTAAACGCCTTATCCTCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGATCGCCTTATCCGCCCTACATGAGCAATCCCGTAg                                                                                                                         >  2:1297043/1‑150 (MQ=255)
                            ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGCACGCCTTATCCGCCCTACATGTGCAATCCCGGAg                                                                                                                         >  1:29588/1‑150 (MQ=255)
                             gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg                                                                                                                        >  1:1477946/1‑150 (MQ=255)
                              cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc                                                                                                                       >  2:737353/1‑150 (MQ=255)
                               ggATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt                                                                                                                      >  2:791813/1‑150 (MQ=255)
                                tattcggcgTAAACGCCTTATCCGCCGTACTTGTGCATGCCCGTAGGCTGGATAAGACGCGTCAAGCGTCGGATCCGGCAATTGCACGGCGCGACTGGCGGATGCGGCTTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg                                                                                                                     <  1:211421/146‑1 (MQ=17)
                                gATGCGGCGTAAACGCCGTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg                                                                                                                     <  2:1198967/150‑1 (MQ=255)
                                 aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATACGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg                                                                                                                    <  1:1303250/150‑1 (MQ=255)
                                    cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa                                                                                                                 <  1:1111145/150‑1 (MQ=255)
                                    cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa                                                                                                                 <  2:1001191/150‑1 (MQ=255)
                                      gcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCGTACATGTGCAATCCCCTAGGCTGGAGaac                                                                                                               >  1:123579/1‑149 (MQ=255)
                                       cgTAAACGCCTTATCCGCCCTACATGTACAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa                                                                                                              <  1:482485/150‑1 (MQ=255)
                                          aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTTGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGc                                                                                                           <  1:988283/150‑1 (MQ=255)
                                          aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCTTAGGCTGGATAAGaatc                                                                                                           >  2:24959/1‑147 (MQ=255)
                                          aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGc                                                                                                           >  2:416303/1‑150 (MQ=255)
                                          aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGc                                                                                                           <  1:590816/150‑1 (MQ=255)
                                          aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGAACCGCGCCACAGGCCGATGCGGCATGAACGCCGAATCCGCCCTACATGTGCAATCCCGTAGGATCGCTAAGAAGc                                                                                                           >  2:284762/1‑150 (MQ=17)
                                            aCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGTAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCgg                                                                                                         >  2:667585/1‑150 (MQ=255)
                                             cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGc                                                                                                        >  2:1117511/1‑150 (MQ=255)
                                             cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGc                                                                                                        >  2:271270/1‑150 (MQ=255)
                                              gacgtATCCGCACTACATGTTCAATCCCGTCGGCTGGATCAGACGCCGCAAGCGTCGAATCCGGTTATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCa                                                                                                       <  2:1050057/146‑1 (MQ=18)
                                              gCCTTATCCGCCCTACATGTGCAATCACGTAGGCTGTATAAGACGCGGCAAGCGTCGCATCCGGCTTTTGGACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCa                                                                                                       <  2:270188/150‑1 (MQ=18)
                                                   aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                                                                                  <  1:886068/150‑1 (MQ=255)
                                                     ccGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCATTTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCg                                                                                                <  2:860390/150‑1 (MQ=255)
                                                     ccGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACAGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCATTCCCGTAGGCTTGATAAGATGCGGCAAGCGTCg                                                                                                <  1:1555289/150‑1 (MQ=255)
                                                      cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACGGGCGGCTGCGGCGTGCACGCCTTATCAGCCCTACATGTGCAATCCCGTAGGCTAGATAACATGCGGCACGCCTCGc                                                                                               >  2:1124124/1‑150 (MQ=18)
                                                           tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACAGGAGGATGCGGCGTGAACGACTTATCAACCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCTTAGCATCCg                                                                                          >  2:270931/1‑150 (MQ=37)
                                                            aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa                                                                                              <  1:780487/145‑1 (MQ=255)
                                                            aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa                                                                                              >  2:780487/1‑145 (MQ=255)
                                                            aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg                                                                                         >  2:1021265/1‑150 (MQ=255)
                                                               tgtgCATTCACGTATGCTGGATAAGACTCGGCAAGCGTCGCATCCGGCAATTGCACCGATCAACTGGCGGATGCGGCGTTAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt                                                                                      <  2:212203/150‑1 (MQ=18)
                                                               tgtgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGACCGCCTTATCCGCCCTTCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt                                                                                      >  1:1177840/1‑150 (MQ=255)
                                                                 tgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAttt                                                                                    <  2:1677588/150‑1 (MQ=255)
                                                                   cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAt                                                                                  <  1:1680038/150‑1 (MQ=255)
                                                                   cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCCTAGGCTGGATAACATGCGCCAATCGTCGCATCCGGCATTTAt                                                                                  >  2:297956/1‑150 (MQ=255)
                                                                       cccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcg                                                                              >  1:1673516/1‑150 (MQ=255)
                                                                        ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcgc                                                                             >  2:832050/1‑150 (MQ=255)
                                                                                 tgATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGAGTGAACGCCTTATACGCCCTACATGTGCAATCACCTAGGCTGGATAAGATGCGGCAAGCGTAGCAACCGGCATTTATTGCGCCCATGCGTa                                                                    >  1:153547/2‑150 (MQ=18)
                                                                                 ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTa                                                                    >  1:910384/1‑150 (MQ=255)
                                                                                    tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATGTATTGCGCCGCTGCGTACAg                                                                 >  1:63582/1‑150 (MQ=255)
                                                                                      aGACGCGGCAAGCGTCGCATCCGGCTATTGCTCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGc                                                               <  2:1451255/150‑1 (MQ=255)
                                                                                      aGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGTATAAGATGCGGCAAGCGTCGCATCCGGCATGTATTGCGCCGCTGCGTCCAGGc                                                               >  1:312707/1‑150 (MQ=255)
                                                                                       gACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGcc                                                              >  2:868546/1‑150 (MQ=255)
                                                                                           cgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACa                                                          <  2:345960/150‑1 (MQ=255)
                                                                                               aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt                                                      <  2:1473129/150‑1 (MQ=255)
                                                                                               aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt                                                      >  2:1254089/1‑150 (MQ=255)
                                                                                               aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt                                                      <  1:737353/150‑1 (MQ=255)
                                                                                                 gCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGc                                                    <  1:1395215/150‑1 (MQ=255)
                                                                                                  cGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCt                                                   >  2:1188667/1‑150 (MQ=255)
                                                                                                    tCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTcc                                                 >  2:1019352/1‑150 (MQ=255)
                                                                                                    tCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTcc                                                 <  2:445622/150‑1 (MQ=255)
                                                                                                     cGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCa                                                >  2:913230/1‑150 (MQ=255)
                                                                                                        aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACTCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGc                                             <  1:778048/150‑1 (MQ=255)
                                                                                                          ccGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTg                                           <  1:1001683/150‑1 (MQ=255)
                                                                                                           cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTgg                                          >  2:83410/1‑150 (MQ=255)
                                                                                                           cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTgg                                          >  2:919151/1‑150 (MQ=255)
                                                                                                           cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTgg                                          >  2:862014/1‑150 (MQ=255)
                                                                                                            ggCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGc                                         >  1:1489195/1‑150 (MQ=255)
                                                                                                             gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCg                                        >  1:352381/1‑150 (MQ=255)
                                                                                                                 ttGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTAc                                    <  1:791813/150‑1 (MQ=255)
                                                                                                                    cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTAcgcg                                 <  1:1123664/150‑1 (MQ=255)
                                                                                                                      ccGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGAGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGAATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCggg                               <  1:1297043/150‑1 (MQ=255)
                                                                                                                          gcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTAc                           <  2:411894/150‑1 (MQ=255)
                                                                                                                              agggCGGATAAGGCGTTCACGCCGCATCCGCCCGACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGCCAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcg                       >  2:708048/3‑150 (MQ=25)
                                                                                                                               tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTCGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcgc                      <  1:284762/150‑1 (MQ=255)
                                                                                                                               tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTgcgc                      <  1:680296/150‑1 (MQ=255)
                                                                                                                                ggCGGATAAGGCGTTCACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCa                     >  1:1125962/1‑150 (MQ=37)
                                                                                                                                 gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAg                    <  1:726784/150‑1 (MQ=255)
                                                                                                                                 gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAg                    >  1:640214/1‑150 (MQ=255)
                                                                                                                                 gCGGATGCGGCGTGAACGCCGTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAg                    <  1:63904/150‑1 (MQ=255)
                                                                                                                                   ggataaggcgTTCACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGAc                  >  2:455251/7‑150 (MQ=37)
                                                                                                                                   ggATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGAc                  <  1:1309454/150‑1 (MQ=255)
                                                                                                                                      tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATATGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGCCaaa               >  1:53185/1‑150 (MQ=255)
                                                                                                                                       gcggcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAat              <  2:901783/150‑1 (MQ=255)
                                                                                                                                          gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATc           <  1:467958/150‑1 (MQ=255)
                                                                                                                                              gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTcc       <  2:82387/150‑1 (MQ=255)
                                                                                                                                              gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTcc       <  1:411311/150‑1 (MQ=255)
                                                                                                                                                 cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGACGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTCCAgc    >  2:1027845/1‑150 (MQ=255)
                                                                                                                                                   ccTTATCCGCCCTACATGTACAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTCCAgcgc  >  1:707624/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTCCAGGCTGGCGGTACGCGGGTTACTGCGCAGACAAATATCTTCCAGCGC  >  NC_000913/2568111‑2568407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: