Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,612,823 T→C 6.4% A308A (GCT→GCC hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,612,8230TC 6.4% 317.9 / 9.3 109A308A (GCT→GCChyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (53/49);  new base C (5/2);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

Only 100 of 111 total aligned reads displayed.

TGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCT  >  NC_000913/2612676‑2612972
                                                                                                                                                   |                                                                                                                                                     
tGCATCTAACGGATGCGAATCTGCGCACCTTATACCGCTTACCTTTGGCAACCATACACCGCGTGCATTGATGCTGGCGCATACCTCTACCACTCACTATAGTGAGGATAACTCCCAGCTACTACAACACAAAGCCGATCGCATCGCTAt                                                                                                                                                     >  1:349311/1‑150 (MQ=255)
 gCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCAAACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCCCTAtt                                                                                                                                                    >  2:1445212/1‑150 (MQ=255)
  cATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTg                                                                                                                                                   <  1:62260/150‑1 (MQ=255)
  cATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTg                                                                                                                                                   <  1:772494/150‑1 (MQ=255)
  cATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTg                                                                                                                                                   <  1:1246476/150‑1 (MQ=255)
   aTCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGc                                                                                                                                                  <  1:786187/150‑1 (MQ=255)
    tCTAACGGCTGTGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTCCTACAACCCATCGCCGATCGCATCGCTATTGcc                                                                                                                                                 >  1:260175/1‑150 (MQ=255)
    tCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGAAACCATACAACGGTTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACACCTCATAGCCGATCGCATCGCTATTGcg                                                                                                                                                 >  1:287923/1‑149 (MQ=255)
       aaCGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGtt                                                                                                                                              <  1:152484/150‑1 (MQ=255)
          ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCTCATACATCTTCCACTCTCTTTAGTGAGCAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                           <  2:346485/150‑1 (MQ=255)
          ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                           <  2:1669346/150‑1 (MQ=255)
          ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                           <  2:450957/150‑1 (MQ=255)
             tgagAATCTGCGCTCCTTATACCGCTTAACATTCGCAACCATACACCGGGTGCATTGTTGCTGGCTCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCGAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAAt                                                                                                                                        <  2:1091259/147‑1 (MQ=255)
             tGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTTGTGAGGTTAACTGCCAGCTACTACAACACATAGCCGCTCGCATCGCTATTGCCGTTGGCAAt                                                                                                                                        >  1:414302/1‑150 (MQ=255)
               cGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGc                                                                                                                                      >  1:366948/1‑150 (MQ=255)
                gAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCg                                                                                                                                    <  2:1364699/150‑1 (MQ=255)
                 aaTCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGccc                                                                                                                                    >  2:431151/1‑149 (MQ=255)
                    cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATg                                                                                                                                 <  1:549763/150‑1 (MQ=255)
                    cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATg                                                                                                                                 <  2:657722/150‑1 (MQ=255)
                     tGCGCTCCTTAAACCTCTTACCTTTGGCAACCATACACCGCGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATGc                                                                                                                                <  2:898110/150‑1 (MQ=255)
                      gcgcTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGcc                                                                                                                               >  1:756003/1‑150 (MQ=255)
                         cTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTgg                                                                                                                            <  1:992775/150‑1 (MQ=255)
                         cTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTgg                                                                                                                            >  2:150196/1‑150 (MQ=255)
                              tataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACTTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAg                                                                                                                       <  1:594317/150‑1 (MQ=255)
                               ataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGc                                                                                                                      <  1:1385986/150‑1 (MQ=255)
                                 aCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCAt                                                                                                                    <  2:774821/150‑1 (MQ=255)
                                     cTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCTGATGCCTGGCGTAGCATGAcc                                                                                                                <  2:281474/150‑1 (MQ=255)
                                         ccTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                            >  1:898816/1‑150 (MQ=255)
                                         ccTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                            >  2:586014/1‑150 (MQ=255)
                                          cTTTGGCAACCATACACCGGGTGCAGTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAttt                                                                                                           <  1:669379/150‑1 (MQ=255)
                                            ccagCAACAATGCACCCGGTGTATGGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGACGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAGTTGc                                                                                                         >  2:20773/4‑150 (MQ=255)
                                             tGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCa                                                                                                        <  1:734084/150‑1 (MQ=255)
                                             tGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCa                                                                                                        >  2:285294/1‑150 (MQ=255)
                                             cagCAACAATGCACCCGGTGTATGGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCa                                                                                                        >  1:1632285/3‑150 (MQ=255)
                                                cAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGa                                                                                                     >  1:375589/1‑150 (MQ=255)
                                                cAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGa                                                                                                     >  1:855017/1‑150 (MQ=255)
                                                cAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGa                                                                                                     >  2:635461/1‑150 (MQ=255)
                                                 aaCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACCACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaa                                                                                                    >  2:1572463/1‑150 (MQ=255)
                                                  aCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaaa                                                                                                   >  2:23298/1‑150 (MQ=255)
                                                  aCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaaa                                                                                                   >  2:17228/1‑150 (MQ=255)
                                                     aTACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGtt                                                                                                <  2:459799/150‑1 (MQ=255)
                                                             ggTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGGTCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCaa                                                                                        >  1:1450892/1‑150 (MQ=255)
                                                                 cATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaa                                                                                    >  2:267717/1‑150 (MQ=255)
                                                                   ttgttgCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAac                                                                                  <  2:1465010/150‑1 (MQ=255)
                                                                          tGGCGCATACCGCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGc                                                                           <  1:673534/150‑1 (MQ=255)
                                                                            gcgcATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCtt                                                                         <  2:570104/150‑1 (MQ=255)
                                                                             cgcATACATCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACTACCAGCTTa                                                                        >  1:1231261/1‑150 (MQ=255)
                                                                                aTACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                     <  1:431151/150‑1 (MQ=255)
                                                                                aTACCTCATCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                     <  2:457113/150‑1 (MQ=255)
                                                                                   ccTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGagc                                                                  >  2:738623/1‑150 (MQ=255)
                                                                                      ctTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGagcagc                                                               <  1:1213359/150‑1 (MQ=255)
                                                                                         ccACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAACCCACCAGCTTAGCGAGCAGCTcc                                                            >  2:707454/1‑150 (MQ=255)
                                                                                          cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCt                                                           >  2:49156/1‑150 (MQ=255)
                                                                                          cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCt                                                           >  2:1699305/1‑150 (MQ=255)
                                                                                              ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCg                                                       >  2:1337590/1‑150 (MQ=255)
                                                                                              ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCg                                                       >  2:782388/1‑150 (MQ=255)
                                                                                               tctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGa                                                      >  2:525115/1‑150 (MQ=255)
                                                                                                ctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                     >  1:503770/1‑134 (MQ=255)
                                                                                                ctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                     <  2:503770/134‑1 (MQ=255)
                                                                                                 tttAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAAt                                                    >  2:1259434/1‑150 (MQ=255)
                                                                                                    aGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTg                                                 >  2:614178/1‑150 (MQ=255)
                                                                                                    aGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTg                                                 >  2:596319/1‑150 (MQ=255)
                                                                                                      tGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTGCGAAGCAggg                                               >  2:830820/1‑150 (MQ=255)
                                                                                                       gAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGc                                              >  1:942511/1‑150 (MQ=255)
                                                                                                                    aGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATta                                 >  2:1221860/1‑150 (MQ=255)
                                                                                                                          tacAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATtatctatc                           <  2:470108/150‑1 (MQ=255)
                                                                                                                           acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                          <  2:364486/150‑1 (MQ=255)
                                                                                                                           acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                          >  2:1013627/1‑150 (MQ=255)
                                                                                                                           acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                          >  2:1425878/1‑150 (MQ=255)
                                                                                                                           acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                          >  2:1419242/1‑150 (MQ=255)
                                                                                                                           acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                          <  1:267717/150‑1 (MQ=255)
                                                                                                                           acaacaCATAGCCGATCGCATCGATATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCTGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                          <  2:1145237/150‑1 (MQ=255)
                                                                                                                            caacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCaa                         >  1:1626847/1‑150 (MQ=255)
                                                                                                                              acacATAGCCGATCGCATCGCTCTTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAACCCACCAGCGTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAg                       <  2:1054820/150‑1 (MQ=255)
                                                                                                                              acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTTTCTATCAAAg                       >  2:1583007/1‑150 (MQ=255)
                                                                                                                               cacaTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGc                      >  2:1640049/1‑150 (MQ=255)
                                                                                                                                 caTAGCCGCTCGCATCGCTATTGCCGTTGGCAATGCCTATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCa                    <  2:138108/150‑1 (MQ=255)
                                                                                                                                  aTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCGTAGGCCTGGCGTAGCATGAGCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAg                   <  2:88462/150‑1 (MQ=255)
                                                                                                                                  aTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAg                   >  1:1391565/1‑150 (MQ=255)
                                                                                                                                    cgCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCGGGCGTAGCATGACCGCTTTGCAGGAAAGTTTGCATCAAGAACACCACCAGCTTAGCGAGCAGCGCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                 <  1:1101545/149‑1 (MQ=255)
                                                                                                                                    aGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGCCCGATTTGCAGGAAAGTTTGCAGCAAGAAACCCACCAGCTTAGCGAGCAGCTCCTTTCGATTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                 >  1:1546103/1‑150 (MQ=255)
                                                                                                                                    aGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                 <  1:1178233/150‑1 (MQ=255)
                                                                                                                                       gccaggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGCCATTATCTATCACCGCCAGGCAAt              >  2:1010687/6‑150 (MQ=37)
                                                                                                                                        gATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATg             <  2:375589/150‑1 (MQ=255)
                                                                                                                                        gATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCACCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGTCATCGGTGACATTATCTCTCAAAGCCAGGCAATg             <  2:115163/150‑1 (MQ=255)
                                                                                                                                          tcgcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGa           <  2:366948/150‑1 (MQ=255)
                                                                                                                                           ggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa          >  2:588188/2‑150 (MQ=255)
                                                                                                                                            gcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAg         <  2:1003367/150‑1 (MQ=255)
                                                                                                                                            gcagcgaTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAg         <  1:286232/150‑1 (MQ=255)
                                                                                                                                             catcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa        >  2:568735/1‑150 (MQ=255)
                                                                                                                                             catcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa        >  2:1333394/1‑150 (MQ=255)
                                                                                                                                             catcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGAAATGGAAGa        >  2:919973/1‑150 (MQ=255)
                                                                                                                                              atcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAc       <  2:260175/150‑1 (MQ=255)
                                                                                                                                               tcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAcc      <  1:635461/150‑1 (MQ=255)
                                                                                                                                               tcgcTAATGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCAGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAcc      <  1:285294/150‑1 (MQ=255)
                                                                                                                                                 gcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg    <  1:23298/150‑1 (MQ=255)
                                                                                                                                                 gcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg    <  1:17228/150‑1 (MQ=255)
                                                                                                                                                  cTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGc   <  2:756003/150‑1 (MQ=255)
                                                                                                                                                   tATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTAGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCt  <  2:1615345/150‑1 (MQ=255)
                                                                                                                                                   tATTGCCGTGGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCt  <  2:1531237/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCT  >  NC_000913/2612676‑2612972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: