Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,612,831 T→A 7.1% V311D (GTT→GAT) ‡ hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,612,8310TA 7.1% 286.1 / 9.2 107V311D (GTT→GAT) ‡hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (52/47);  new base A (6/2);  total (58/49)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 108 total aligned reads displayed.

AACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAG  >  NC_000913/2612683‑2612979
                                                                                                                                                    |                                                                                                                                                    
aaCGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGtt                                                                                                                                                     <  1:152484/150‑1 (MQ=255)
   ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCTCATACATCTTCCACTCTCTTTAGTGAGCAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                                  <  2:346485/150‑1 (MQ=255)
   ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                                  <  2:450957/150‑1 (MQ=255)
   ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                                  <  2:1669346/150‑1 (MQ=255)
      tgagAATCTGCGCTCCTTATACCGCTTAACATTCGCAACCATACACCGGGTGCATTGTTGCTGGCTCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCGAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAAt                                                                                                                                               <  2:1091259/147‑1 (MQ=255)
      tGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTTGTGAGGTTAACTGCCAGCTACTACAACACATAGCCGCTCGCATCGCTATTGCCGTTGGCAAt                                                                                                                                               >  1:414302/1‑150 (MQ=255)
        cGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGc                                                                                                                                             >  1:630467/1‑150 (MQ=255)
        cGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGc                                                                                                                                             >  1:366948/1‑150 (MQ=255)
             cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATg                                                                                                                                        <  1:549763/150‑1 (MQ=255)
             cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATg                                                                                                                                        <  2:657722/150‑1 (MQ=255)
              tGCGCTCCTTAAACCTCTTACCTTTGGCAACCATACACCGCGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATGc                                                                                                                                       <  2:898110/150‑1 (MQ=255)
               gcgcTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGcc                                                                                                                                      >  1:756003/1‑150 (MQ=255)
                  cTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTgg                                                                                                                                   >  2:150196/1‑150 (MQ=255)
                       tatcCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAg                                                                                                                              <  1:1614623/150‑1 (MQ=255)
                       tataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACTTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAg                                                                                                                              <  1:594317/150‑1 (MQ=255)
                        ataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGc                                                                                                                             <  1:1385986/150‑1 (MQ=255)
                          aCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCAt                                                                                                                           <  2:774821/150‑1 (MQ=255)
                              cTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCTGATGCCTGGCGTAGCATGAcc                                                                                                                       <  2:281474/150‑1 (MQ=255)
                                  ccTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                                   >  1:898816/1‑150 (MQ=255)
                                  ccTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                                   >  2:586014/1‑150 (MQ=255)
                                   cTTTGGCAACCATACACCGGGTGCAGTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAttt                                                                                                                  <  1:669379/150‑1 (MQ=255)
                                     ccagCAACAATGCACCCGGTGTATGGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGACGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAGTTGc                                                                                                                >  2:20773/4‑150 (MQ=255)
                                      tGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCa                                                                                                               >  2:285294/1‑150 (MQ=255)
                                      tGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCa                                                                                                               <  1:734084/150‑1 (MQ=255)
                                      cagCAACAATGCACCCGGTGTATGGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCa                                                                                                               >  1:1632285/3‑150 (MQ=255)
                                         cAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGa                                                                                                            >  1:375589/1‑150 (MQ=255)
                                         cAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGa                                                                                                            >  2:635461/1‑150 (MQ=255)
                                         cAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGa                                                                                                            >  1:855017/1‑150 (MQ=255)
                                          aaCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACCACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaa                                                                                                           >  2:1572463/1‑150 (MQ=255)
                                           aCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaaa                                                                                                          >  2:17228/1‑150 (MQ=255)
                                           aCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaaa                                                                                                          >  2:23298/1‑150 (MQ=255)
                                              aTACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGtt                                                                                                       <  2:459799/150‑1 (MQ=255)
                                                      ggTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGGTCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCaa                                                                                               >  1:1450892/1‑150 (MQ=255)
                                                          cATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaa                                                                                           >  2:267717/1‑150 (MQ=255)
                                                          cATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaa                                                                                           >  2:365635/1‑150 (MQ=255)
                                                           aTTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGCTCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaaa                                                                                          <  2:1450892/150‑1 (MQ=255)
                                                            ttgttgCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAac                                                                                         <  2:1465010/150‑1 (MQ=255)
                                                                 gCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGACAACTGCCAGCTACTACACCACATAGCCGATCGCATCGCTATTGCCGATGGCAATGCCGATGCCAGGCGTAGCATTCCCGAATTGCAGGAAAGTTTGCAGCAAGAAAaccaccc                                                                                    >  1:938672/1‑149 (MQ=255)
                                                                   tGGCGCATACCGCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGc                                                                                  <  1:673534/150‑1 (MQ=255)
                                                                     gcgcATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCtt                                                                                <  2:570104/150‑1 (MQ=255)
                                                                      cgcATACATCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACTACCAGCTTa                                                                               >  1:1231261/1‑150 (MQ=255)
                                                                         aTACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                            <  1:431151/150‑1 (MQ=255)
                                                                         aTACCTCATCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                            <  2:457113/150‑1 (MQ=255)
                                                                            ccTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGagc                                                                         >  2:738623/1‑150 (MQ=255)
                                                                               ctTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGagcagc                                                                      <  1:1213359/150‑1 (MQ=255)
                                                                                  ccACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAACCCACCAGCTTAGCGAGCAGCTcc                                                                   >  2:707454/1‑150 (MQ=255)
                                                                                   cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCt                                                                  >  2:49156/1‑150 (MQ=255)
                                                                                   cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCt                                                                  >  2:1699305/1‑150 (MQ=255)
                                                                                       ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCg                                                              >  2:1337590/1‑150 (MQ=255)
                                                                                       ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCg                                                              >  2:782388/1‑150 (MQ=255)
                                                                                        tctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGa                                                             >  2:525115/1‑150 (MQ=255)
                                                                                         ctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCg                                                                            <  2:503770/134‑1 (MQ=255)
                                                                                          tttAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAAt                                                           >  2:1259434/1‑150 (MQ=255)
                                                                                             aGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTg                                                        >  2:596319/1‑150 (MQ=255)
                                                                                               tGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTGCGAAGCAggg                                                      >  2:830820/1‑150 (MQ=255)
                                                                                                gAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGc                                                     >  1:942511/1‑150 (MQ=255)
                                                                                                             aGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGCCCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCGTGTCGATTCTGGGCATCGGTGACATta                                        >  1:334096/1‑150 (MQ=255)
                                                                                                             aGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATta                                        >  2:1221860/1‑150 (MQ=255)
                                                                                                                   tacAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATtatctatc                                  <  2:470108/150‑1 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                 >  2:1419242/1‑150 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                 <  2:364486/150‑1 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                 <  1:267717/150‑1 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                 >  2:1013627/1‑150 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                 >  2:1425878/1‑150 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGATATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCTGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                 <  2:1145237/150‑1 (MQ=255)
                                                                                                                     caacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCaa                                >  1:1626847/1‑150 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTCTTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAACCCACCAGCGTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAg                              <  2:1054820/150‑1 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTTTCTATCAAAg                              >  2:1583007/1‑150 (MQ=255)
                                                                                                                        cacaTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGc                             >  2:1640049/1‑150 (MQ=255)
                                                                                                                          caTAGCCGCTCGCATCGCTATTGCCGTTGGCAATGCCTATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCa                           <  2:138108/150‑1 (MQ=255)
                                                                                                                          caTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGCGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCa                           >  2:513175/1‑150 (MQ=255)
                                                                                                                           aTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCGTAGGCCTGGCGTAGCATGAGCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAg                          <  2:88462/150‑1 (MQ=255)
                                                                                                                           aTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAg                          >  1:1391565/1‑150 (MQ=255)
                                                                                                                             cgCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCGGGCGTAGCATGACCGCTTTGCAGGAAAGTTTGCATCAAGAACACCACCAGCTTAGCGAGCAGCGCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                        <  1:1101545/149‑1 (MQ=255)
                                                                                                                             aGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGCCCGATTTGCAGGAAAGTTTGCAGCAAGAAACCCACCAGCTTAGCGAGCAGCTCCTTTCGATTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                        >  1:1546103/1‑150 (MQ=255)
                                                                                                                             aGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                        <  1:1178233/150‑1 (MQ=255)
                                                                                                                                gccaggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGCCATTATCTATCACCGCCAGGCAAt                     >  2:1010687/6‑150 (MQ=37)
                                                                                                                                 gATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATg                    <  2:375589/150‑1 (MQ=255)
                                                                                                                                 gATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCACCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGTCATCGGTGACATTATCTCTCAAAGCCAGGCAATg                    <  2:115163/150‑1 (MQ=255)
                                                                                                                                   tcgcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGa                  <  2:366948/150‑1 (MQ=255)
                                                                                                                                    ggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                 >  2:669879/2‑150 (MQ=255)
                                                                                                                                    ggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                 >  2:588188/2‑150 (MQ=255)
                                                                                                                                     gcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAg                <  2:1003367/150‑1 (MQ=255)
                                                                                                                                     gcagcgaTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAg                <  1:286232/150‑1 (MQ=255)
                                                                                                                                      catcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa               >  2:1333394/1‑150 (MQ=255)
                                                                                                                                      catcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa               >  2:568735/1‑150 (MQ=255)
                                                                                                                                      catcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGAAATGGAAGa               >  2:919973/1‑150 (MQ=255)
                                                                                                                                       atcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAc              <  2:260175/150‑1 (MQ=255)
                                                                                                                                        tcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAcc             <  1:635461/150‑1 (MQ=255)
                                                                                                                                        tcgcTAATGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCAGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAcc             <  1:285294/150‑1 (MQ=255)
                                                                                                                                          gcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg           <  1:23298/150‑1 (MQ=255)
                                                                                                                                          gcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg           <  1:17228/150‑1 (MQ=255)
                                                                                                                                           cTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGc          <  2:756003/150‑1 (MQ=255)
                                                                                                                                            tATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTAGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCt         <  2:1615345/150‑1 (MQ=255)
                                                                                                                                            tATTGCCGTGGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCt         <  2:1531237/150‑1 (MQ=255)
                                                                                                                                             aTTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCTATTTGCAGGAAAGTTTGCAGCAAGAAAACTACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTc        <  2:1231261/150‑1 (MQ=255)
                                                                                                                                             aTTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTc        >  1:43255/1‑150 (MQ=255)
                                                                                                                                                  cGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagca   <  1:1445212/150‑1 (MQ=255)
                                                                                                                                                   gTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagcag  >  1:662368/1‑150 (MQ=255)
                                                                                                                                                   gTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagcag  >  1:1700569/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAG  >  NC_000913/2612683‑2612979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: