Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,630,918 A→T 5.2% intergenic (+53/+40) yfgI → / ← guaA nalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,630,9180AT 5.2% 337.3 / 5.4 116intergenic (+53/+40)yfgI/guaAnalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base A (52/58);  new base T (2/4);  total (54/62)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 119 total aligned reads displayed.

GCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCG  >  NC_000913/2630770‑2631066
                                                                                                                                                    |                                                                                                                                                    
gCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTgtag                                                                                                                                                     >  2:450384/1‑150 (MQ=255)
  tGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTAGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTgtagta                                                                                                                                                   <  1:680093/150‑1 (MQ=255)
    aCGCCAACAGAAATATGACTGAATTCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGATAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAAc                                                                                                                                                 <  1:1622700/150‑1 (MQ=255)
     cGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACa                                                                                                                                                >  1:703979/1‑150 (MQ=255)
        cAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagag                                                                                                                                             <  2:1440358/150‑1 (MQ=255)
        cAACAGAAAAATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTATAATTAATCGATGATAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagac                                                                                                                                             >  1:1187235/1‑149 (MQ=255)
          aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAggg                                                                                                                                           <  1:804295/150‑1 (MQ=255)
          aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAggg                                                                                                                                           <  1:385760/150‑1 (MQ=255)
           cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                          <  1:780343/150‑1 (MQ=255)
           cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGt                                                                                                                                          <  2:801942/150‑1 (MQ=255)
           cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTCCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                          <  2:1561492/150‑1 (MQ=255)
             gAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGttt                                                                                                                                        <  1:462891/150‑1 (MQ=255)
               aaTATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTg                                                                                                                                      <  2:1424594/150‑1 (MQ=255)
                atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGt                                                                                                                                     <  1:44770/150‑1 (MQ=255)
                atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACCTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGt                                                                                                                                     <  1:1400851/150‑1 (MQ=255)
                  atGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc                                                                                                                                   >  1:86283/1‑150 (MQ=255)
                    gACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                 >  1:378289/1‑150 (MQ=255)
                    gACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                 >  1:1237015/1‑150 (MQ=255)
                     aCTGTAGGCGGTCGCGATCTGATTAAAAGGGTTGGCAAATCAGAAGAGAAGAAATCGGACAGCTTTTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGATGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcatt                                                                                                                                <  2:1307215/150‑1 (MQ=255)
                      cTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGt                                                                                                                                          >  1:1529143/1‑139 (MQ=255)
                       tGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcattca                                                                                                                              >  1:932916/1‑150 (MQ=255)
                        gAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTtcattcat                                                                                                                             >  1:183602/1‑150 (MQ=255)
                        gAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTACAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTtcattcat                                                                                                                             >  1:850919/1‑150 (MQ=255)
                         aaGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATa                                                                                                                            <  1:941944/150‑1 (MQ=255)
                          aGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAg                                                                                                                           <  2:47311/150‑1 (MQ=255)
                              ggTCGCGATCTGATGAAAAGCGTGTGCAAAGCAGAAGAGAACAAATCGTACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCa                                                                                                                       <  2:1630507/150‑1 (MQ=255)
                                   cgATCTTATGAAAAGCTTGTGAAAAGCAGAAGATAACAAATCGGACAGATGGTTTAATTAATGGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTc                                                                                                                  <  2:497386/150‑1 (MQ=255)
                                        tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                             <  2:1366792/150‑1 (MQ=255)
                                        tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                             >  1:902303/1‑150 (MQ=255)
                                          atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCAt                                                                                                           >  2:419906/1‑150 (MQ=255)
                                           tgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCAtt                                                                                                          >  1:1110899/1‑150 (MQ=255)
                                                aGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCAc                                                                                                     >  2:474161/1‑150 (MQ=255)
                                                 gCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACt                                                                                                    >  2:1948/1‑150 (MQ=255)
                                                  cGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTc                                                                                                   <  2:615259/150‑1 (MQ=255)
                                                     gggCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTAAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAATTCATTCCCACTCAAt                                                                                                >  2:1001108/1‑150 (MQ=255)
                                                      ggCAAAGCAGAAGAGAACAAATCGTACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAACTCATTCCCACTCAATg                                                                                               <  2:796912/150‑1 (MQ=255)
                                                      ggCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATg                                                                                               <  1:1644655/150‑1 (MQ=255)
                                                       gCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATgg                                                                                              >  2:453783/1‑150 (MQ=255)
                                                            gCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCt                                                                                         <  1:1550370/150‑1 (MQ=255)
                                                            gCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGGCAAATCATTCCCACTCAATGGTAGCt                                                                                         <  2:1240439/150‑1 (MQ=255)
                                                                  gagaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTggcggc                                                                                   <  1:394007/150‑1 (MQ=255)
                                                                     aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTg                                                                                >  1:1306538/1‑150 (MQ=255)
                                                                      aCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGc                                                                               <  2:967084/150‑1 (MQ=255)
                                                                        aaaTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCg                                                                             >  2:914285/1‑150 (MQ=255)
                                                                        aaaTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCg                                                                             >  2:1607060/1‑150 (MQ=255)
                                                                        aaaTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCg                                                                             >  2:906365/1‑150 (MQ=255)
                                                                        aaaTCGGACAGATGGTTTAATTCATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCg                                                                             <  2:1056195/150‑1 (MQ=255)
                                                                         aaTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGc                                                                            >  2:1140339/1‑150 (MQ=255)
                                                                          aTCGGACAGCTGGTTTAATTAAACGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCt                                                                           <  2:314255/150‑1 (MQ=255)
                                                                           tCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTg                                                                          <  2:465372/150‑1 (MQ=255)
                                                                            cGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGTGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGTTAGCTGGCGGCTTGCCGCTGa                                                                         >  1:1099335/1‑150 (MQ=255)
                                                                             ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAACACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAt                                                                        <  1:461612/150‑1 (MQ=255)
                                                                             ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAt                                                                        >  2:764809/1‑150 (MQ=255)
                                                                             ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAt                                                                        >  1:1236284/1‑150 (MQ=255)
                                                                                cAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTc                                                                     <  2:1567376/150‑1 (MQ=255)
                                                                                cAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTAAAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTAATTCATAGTGCAGGTTCAAATGATTCCCACTCAATGGTCGCTGGCGGCTTGCCGCTGATGTc                                                                     >  1:251799/1‑150 (MQ=255)
                                                                                 aGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCa                                                                    <  2:1481277/150‑1 (MQ=255)
                                                                                  gCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCAt                                                                   >  1:636381/1‑150 (MQ=255)
                                                                                    tGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATac                                                                 <  2:249090/150‑1 (MQ=255)
                                                                                    tGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTAGCGGCTTGCCGCTGATGTCATac                                                                 <  1:40505/150‑1 (MQ=255)
                                                                                            ttaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCAcgcg                                                         >  2:1510142/1‑150 (MQ=255)
                                                                                             taatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCgg                                                        >  2:387353/1‑150 (MQ=255)
                                                                                                 cGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGaaa                                                    >  2:1015279/1‑150 (MQ=255)
                                                                                                  gATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAAt                                                   >  2:1219851/1‑150 (MQ=255)
                                                                                                       tAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGt                                              >  1:598940/1‑150 (MQ=255)
                                                                                                       tAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGt                                              <  2:932916/150‑1 (MQ=255)
                                                                                                        aGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGtt                                             >  2:770032/1‑150 (MQ=255)
                                                                                                         gTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTc                                            <  2:313003/150‑1 (MQ=255)
                                                                                                           aaCTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCAc                                          <  2:1110899/150‑1 (MQ=255)
                                                                                                            aCTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGTGAAATACCGTTCACt                                         >  1:1451610/1‑150 (MQ=255)
                                                                                                               tCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAATTACCGTTCACTTCa                                      >  2:1147680/1‑150 (MQ=255)
                                                                                                                cAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCGGATGTCATACACCACGCGGGAAATACCGTTCACTTCAt                                     <  1:254264/150‑1 (MQ=255)
                                                                                                                cAATACTATAATTCTTGATGATAAAAAACCCTCTGTAGTAACATATGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCTACTCAATGGTAGCTGGAGGATTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAt                                     <  1:272442/150‑1 (MQ=255)
                                                                                                                 aaTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                    <  1:363240/150‑1 (MQ=255)
                                                                                                                  aTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTg                                   <  2:43661/150‑1 (MQ=255)
                                                                                                                    ccTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGTTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGAt                                 <  2:1187235/150‑1 (MQ=255)
                                                                                                                     cTATAATTCTTGAAGATAAAATACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGGCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATa                                <  1:163749/150‑1 (MQ=255)
                                                                                                                     cTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATa                                <  1:450384/150‑1 (MQ=255)
                                                                                                                     atataATTATTGAAGATAAAAAACACTCTGTAGTAACATTGGGTTTTGTTCATTCATAGTGCAGTGTCAAATCATTCCCACTCAATGGTAGCTGGCGGATTGCCGCTGATGTCATACACCACGCGTGAAATACCGTTCACTTCATTGATa                                <  2:406755/149‑1 (MQ=255)
                                                                                                                         aaTTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCAAGCGGGAAATACAGTTCACTTCATTGATAATGc                            >  2:13419/1‑150 (MQ=255)
                                                                                                                             cTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                        >  1:1243875/1‑150 (MQ=255)
                                                                                                                              ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTAAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTg                       <  1:1096652/150‑1 (MQ=255)
                                                                                                                                 aaGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCTTTCATAGTGCAGGGTCAAATCATTCCAACTCAATGGTAGCTGGCGGATTGCCGCTGATGTAATACACCACGCGGGAAATACCGTTCACTGCATTGATTATGCGGTTGGaa                    >  1:1584636/1‑150 (MQ=255)
                                                                                                                                 aaGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGCGAAATACCGTTCACTTCATTGATAATGCGGTTGGaa                    >  1:1036845/1‑150 (MQ=255)
                                                                                                                                  aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCg                                               <  1:1130616/122‑1 (MQ=255)
                                                                                                                                  aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCg                                               >  2:1130616/1‑122 (MQ=255)
                                                                                                                                  aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGaaa                   >  1:1581075/1‑150 (MQ=255)
                                                                                                                                  aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGaaa                   >  2:1729483/1‑150 (MQ=255)
                                                                                                                                  aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATACCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGATATACCGTTCACTTCATTGATAATGCGGTTGGaaa                   >  2:1398684/1‑150 (MQ=255)
                                                                                                                                   gATAAAAAACCCTCTGTAGTAACAGCGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAGGTCATACCACACGCGGGAAATACCGTTCACTTCATTGTTAATTCGGTTGGAAAc                  >  1:309050/1‑150 (MQ=255)
                                                                                                                                   gATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAc                  >  1:441238/1‑150 (MQ=255)
                                                                                                                                   gATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAc                  <  1:764809/150‑1 (MQ=255)
                                                                                                                                      aaaaaaCCCTCTGTTGTAACAGAGGGTTTTGTTCATTCATCGTGCAGGGTCAAATCATTCCCACTCTATGGTAGCTGGCGACTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg               <  2:842077/150‑1 (MQ=255)
                                                                                                                                      aaaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg               <  2:378289/150‑1 (MQ=255)
                                                                                                                                      aaaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg               <  2:1237015/150‑1 (MQ=255)
                                                                                                                                        aaaaCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcgcc             >  1:1251055/1‑148 (MQ=255)
                                                                                                                                          aaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCg           >  2:873376/1‑150 (MQ=255)
                                                                                                                                             ccTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAgg        >  1:283399/1‑150 (MQ=255)
                                                                                                                                                ctGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCGGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGaaa     <  1:764471/150‑1 (MQ=255)
                                                                                                                                                   tagtaACAGTGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGTAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCg  <  1:1419383/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCG  >  NC_000913/2630770‑2631066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑