Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,632,584 A→T 5.1% intergenic (‑49/+20) guaA ← / ← guaB GMP synthetase (glutamine aminotransferase)/IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,632,5840AT 5.1% 326.8 / 4.7 117intergenic (‑49/+20)guaA/guaBGMP synthetase (glutamine aminotransferase)/IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (51/59);  new base T (3/3);  total (54/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

Only 100 of 118 total aligned reads displayed.

CAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCA  >  NC_000913/2632435‑2632730
                                                                                                                                                     |                                                                                                                                                   
 aGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACAt                                                                                                                                                    <  2:987432/150‑1 (MQ=255)
  gTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGAAAGTGAAACAGATAATATAAATCGCCCGACATg                                                                                                                                                   >  1:436895/1‑150 (MQ=255)
    aaaCACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGaa                                                                                                                                                 >  1:1038146/1‑150 (MQ=255)
         cccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                                                                           >  2:603916/1‑150 (MQ=255)
         cccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                                                                           >  2:1599241/1‑150 (MQ=255)
           cAGCGCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGc                                                                                                                                         <  1:1043999/150‑1 (MQ=255)
            aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTCTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                        <  1:231735/150‑1 (MQ=255)
              cTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGaa                                                                                                                                      >  2:137103/1‑150 (MQ=255)
               tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatt                                                                                                                                     <  1:1633064/150‑3 (MQ=255)
               tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatt                                                                                                                                     <  1:540803/150‑3 (MQ=255)
               tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAag                                                                                                                                     <  2:810953/150‑1 (MQ=255)
                cacgcaccCGGCGCGCAACCAGGTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAaga                                                                                                                                    <  1:1064337/150‑1 (MQ=255)
                   gcacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGaa                                                                                                                                 <  1:170436/150‑1 (MQ=255)
                    cacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAt                                                                                                                                >  2:1085296/1‑150 (MQ=255)
                     acgcGGCGCGCAACCATTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATc                                                                                                                               <  2:712364/150‑1 (MQ=255)
                      cgcgGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCa                                                                                                                              >  1:32188/1‑150 (MQ=255)
                        cggcgCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAgg                                                                                                                            <  1:862496/150‑1 (MQ=255)
                              gcAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGCCGAAGAGAATCAGGAGCCCa                                                                                                                      >  1:1615894/1‑150 (MQ=255)
                                 aCCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGAc                                                                                                                   >  1:723607/1‑150 (MQ=255)
                                       tGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACTGTAGt                                                                                                             >  1:196341/1‑150 (MQ=255)
                                        gAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGCCGGTAGtt                                                                                                            >  1:392810/1‑150 (MQ=255)
                                             tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAg                                                                                                              <  2:668477/143‑1 (MQ=255)
                                             tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                                                                       <  2:1685259/150‑1 (MQ=255)
                                                tGAGAACCGAAGTCCATAATGAGCATGCGAGGCATATGAATGTTTTCCGTCATTTACGCTTATTCCGAGGCAAGTTAAACAGATAATATAAATCGCCCGACATGACCTCGGGCGAAGAGAATAAGTAGCCCAGACGGTAGTTCTGGGAct                                                                                                    <  2:183326/150‑1 (MQ=255)
                                                tGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGAct                                                                                                    >  1:666540/1‑150 (MQ=255)
                                                   gaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCtt                                                                                                 >  2:148495/1‑150 (MQ=255)
                                                    aaCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                                                                <  2:195195/150‑1 (MQ=255)
                                                    aaCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                                                                <  2:511171/150‑1 (MQ=255)
                                                      ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAATTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                              <  2:1077921/150‑1 (MQ=255)
                                                      ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGGAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                              <  1:396130/150‑1 (MQ=255)
                                                      ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGAGGACTCTTTAg                                                                                              <  2:156217/150‑1 (MQ=255)
                                                        gAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGCCGGTAGTTCGGGGACTCTTTAGTa                                                                                            >  1:92011/1‑150 (MQ=255)
                                                         aaGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTaa                                                                                           <  1:1326546/150‑1 (MQ=255)
                                                         aaGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTaa                                                                                           <  1:1319444/150‑1 (MQ=255)
                                                           gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                         <  2:1655503/150‑1 (MQ=255)
                                                           gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                         <  2:1600919/150‑1 (MQ=255)
                                                           gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                         >  2:74356/1‑150 (MQ=255)
                                                            tCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                                                                        <  1:74356/150‑1 (MQ=255)
                                                             ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGt                                                                                       >  2:795667/1‑150 (MQ=255)
                                                              cAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTc                                                                                      >  2:653272/1‑150 (MQ=255)
                                                               aGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCa                                                                                     >  2:1621719/1‑150 (MQ=255)
                                                                 aaTGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGTGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcg                                                                                   <  2:896743/150‑1 (MQ=255)
                                                                     aGGATGCGATGCATATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                                                               <  2:1412782/150‑1 (MQ=255)
                                                                      ggATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAAAATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGTGACTCTTTAGTAATGGTCACGTCGTg                                                                              <  2:469838/150‑1 (MQ=255)
                                                                       gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCATGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGATTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                             <  2:583968/150‑1 (MQ=255)
                                                                          gcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACg                                                                          <  2:1636194/150‑1 (MQ=255)
                                                                           cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTGTTTAGTAATGGTCACGTCGTGAACGt                                                                         >  2:1536967/1‑150 (MQ=255)
                                                                           cgatgcATATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGt                                                                         <  2:351349/150‑1 (MQ=255)
                                                                            gatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTg                                                                        <  1:1621719/150‑1 (MQ=255)
                                                                             atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTgg                                                                       <  2:417941/150‑1 (MQ=255)
                                                                               gcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCACAGACGGTAGTTCTGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCt                                                                     >  1:148295/1‑150 (MQ=255)
                                                                                  tATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTc                                                                  >  2:747026/1‑150 (MQ=255)
                                                                                     taaTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                                                               <  2:740750/149‑1 (MQ=255)
                                                                                       aTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                                             >  1:1042481/1‑150 (MQ=255)
                                                                                        ggTTTTCCGGCATTGACGCTTATTCCGAGGCCAGTGACACAGATAATATAATTCGCCCGACCTGTAGTCGGGCGAAGAGAATCAGGAGCCCAGTCGGTAGTTCTTGTACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAAt                                                            <  1:254767/149‑1 (MQ=255)
                                                                                              ccGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCATTCAGGAAAGccacg                                                      <  2:1258142/150‑4 (MQ=255)
                                                                                                    gtgtcGCTTATTCCGACGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTGCGGGGACTCTCTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGcg                                                <  1:137178/146‑2 (MQ=255)
                                                                                                      gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCTGGCGAAGAGAATCAGGAGCCAAGACGGTAGTTCGGTGACTCTTTAGTAATTGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGa                                              >  1:1043394/1‑150 (MQ=255)
                                                                                                      gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAACAGGAGCCCAGCCGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTGACTGAATGACCGCACCGCTGa                                              >  1:857536/1‑150 (MQ=255)
                                                                                                       aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                             <  1:747026/150‑1 (MQ=255)
                                                                                                       aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                             <  2:1561632/150‑1 (MQ=255)
                                                                                                       aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGGGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                             <  2:116193/150‑1 (MQ=255)
                                                                                                       aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATCAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                             <  1:671936/150‑1 (MQ=255)
                                                                                                         gCTTAATCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATAc                                           <  1:1136945/150‑1 (MQ=255)
                                                                                                            tATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTa                                        <  2:1701688/150‑1 (MQ=255)
                                                                                                              ttCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACa                                      >  2:297602/1‑150 (MQ=255)
                                                                                                               tCCGATGCAAGTGAATCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTACGGGGACTCTTTAGTAATGGTCACGTCGAGAACGAGGCTTTCCTGAATGCCCGCACCTCTCATAAGTaaaa                                     >  1:59746/1‑150 (MQ=255)
                                                                                                               tCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACaa                                     <  1:603916/150‑1 (MQ=255)
                                                                                                               tCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACaa                                     >  1:810762/1‑150 (MQ=255)
                                                                                                                  gAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCAAGACGGTAGTTCGGGGACTCTTTAGTAATGGTAACGTCGTGAACGAGGCTATCCTTAATGCCCGCACCGATGATACGTACAAACt                                  >  1:574423/1‑150 (MQ=255)
                                                                                                                   aGGCAAGTTACACAGATAATATAAATGGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTc                                 <  1:137103/150‑1 (MQ=255)
                                                                                                                   aGGCAAGTGAAACAGATAATATAAATCGCCCTACATGATGTCGGGCGAAGAGCAGCAGGAGCCCAGACTGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGTCCGCACCGCTGATTCGTACTAACTc                                 <  2:291270/150‑1 (MQ=255)
                                                                                                                    gtcAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTTAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                <  2:428328/148‑1 (MQ=255)
                                                                                                                    ggCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                >  1:137313/1‑150 (MQ=255)
                                                                                                                    ggCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                <  2:1303693/150‑1 (MQ=255)
                                                                                                                    gacAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                >  2:1201312/3‑150 (MQ=255)
                                                                                                                         gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                           <  1:800204/150‑1 (MQ=255)
                                                                                                                         gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                           >  2:366591/1‑150 (MQ=255)
                                                                                                                         gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                           >  2:881923/1‑150 (MQ=255)
                                                                                                                          tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                           <  1:1486234/150‑1 (MQ=255)
                                                                                                                          tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACTGTAGTTCGTTGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTa                          <  1:1085296/150‑1 (MQ=255)
                                                                                                                             aaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTa                       <  1:1031180/150‑1 (MQ=255)
                                                                                                                               cAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAACCTCCGCTTTAGTACg                     >  1:1025948/1‑150 (MQ=255)
                                                                                                                               cAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                     >  2:1353169/1‑150 (MQ=255)
                                                                                                                               cAGATAATATAAAACGCCCGACATCACGTCGGGCGAAGAGACTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                     <  1:864704/150‑1 (MQ=255)
                                                                                                                                  ataatataatCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCATGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGt                 >  2:655224/1‑150 (MQ=255)
                                                                                                                                     atatAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACATACAAACTCCGCTTTAGTACGCAGttg               >  1:987841/1‑149 (MQ=255)
                                                                                                                                      tataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcg              >  1:372236/1‑150 (MQ=255)
                                                                                                                                       ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgt             >  2:1352056/1‑150 (MQ=255)
                                                                                                                                        taAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGTGCACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTGTTGTAGGGAGTtcgtg            >  1:442052/1‑149 (MQ=255)
                                                                                                                                         tcttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCTGACCGGAGTTCGGGGACTCATAAGTAATGCTCAGGTCGTGGACGTCGCGTTCCAGCAAGCACCCACCGCTGTTACGTACAAACGCCGCTTTATGAGGCAGTtggtcc           >  2:331566/4‑149 (MQ=255)
                                                                                                                                         aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtcg           >  2:1488988/1‑150 (MQ=255)
                                                                                                                                          cttCGCCCGACTTAATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGa          >  1:1070609/3‑150 (MQ=255)
                                                                                                                                           aTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTTGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGGTCGTCGAt         >  2:88007/1‑150 (MQ=255)
                                                                                                                                             cGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGGTTTAGTACGCAGTTCGTCGATat       >  2:905463/1‑149 (MQ=255)
                                                                                                                                             cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAAc                                   <  2:289713/122‑1 (MQ=255)
                                                                                                                                             cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAAc                                   >  1:289713/1‑122 (MQ=255)
                                                                                                                                             cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAg       >  1:1335380/1‑150 (MQ=255)
                                                                                                                                                ccGACATGAAGGCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTAc    <  1:1421240/150‑1 (MQ=255)
                                                                                                                                                  gACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACca  >  1:1472685/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCA  >  NC_000913/2632435‑2632730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: