Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,632,586 G→C 6.1% intergenic (‑51/+18) guaA ← / ← guaB GMP synthetase (glutamine aminotransferase)/IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,632,5860GC 6.1% 350.1 / 5.6 116intergenic (‑51/+18)guaA/guaBGMP synthetase (glutamine aminotransferase)/IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (51/57);  new base C (2/5);  total (54/62)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCC  >  NC_000913/2632437‑2632735
                                                                                                                                                     |                                                                                                                                                      
gTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGAAAGTGAAACAGATAATATAAATCGCCCGACATg                                                                                                                                                        >  1:436895/1‑150 (MQ=255)
  aaaCACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGaa                                                                                                                                                      >  1:1038146/1‑150 (MQ=255)
      acCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCg                                                                                                                                                 >  2:1310458/1‑150 (MQ=255)
       cccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                                                                                >  2:603916/1‑150 (MQ=255)
         cAGCGCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGc                                                                                                                                              <  1:1043999/150‑1 (MQ=255)
          aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                             <  1:320921/150‑1 (MQ=255)
          aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTCTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                             <  1:231735/150‑1 (MQ=255)
            cTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGaa                                                                                                                                           >  2:137103/1‑150 (MQ=255)
             tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatt                                                                                                                                          <  1:1633064/150‑3 (MQ=255)
             tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatt                                                                                                                                          <  1:540803/150‑3 (MQ=255)
              cacgcaccCGGCGCGCAACCAGGTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAaga                                                                                                                                         <  1:1064337/150‑1 (MQ=255)
                 gcacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGaa                                                                                                                                      <  1:170436/150‑1 (MQ=255)
                  cacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAt                                                                                                                                     >  2:1085296/1‑150 (MQ=255)
                   acgcGGCGCGCAACCATTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATc                                                                                                                                    <  2:712364/150‑1 (MQ=255)
                    cgcgGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCa                                                                                                                                   >  1:32188/1‑150 (MQ=255)
                      cggcgCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAgg                                                                                                                                 <  1:862496/150‑1 (MQ=255)
                           cgcAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGccc                                                                                                                            <  2:1074952/150‑1 (MQ=255)
                            gcAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGCCGAAGAGAATCAGGAGCCCa                                                                                                                           >  1:1615894/1‑150 (MQ=255)
                               aCCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGAc                                                                                                                        >  1:723607/1‑150 (MQ=255)
                                     tGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACTGTAGt                                                                                                                  >  1:196341/1‑150 (MQ=255)
                                      gAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGCCGGTAGtt                                                                                                                 >  1:392810/1‑150 (MQ=255)
                                           tACTGAGAACCGAAGTCCAGAATGAGTATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATGCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                                                                            <  1:827029/150‑1 (MQ=255)
                                           tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAg                                                                                                                   >  1:668477/1‑143 (MQ=255)
                                           tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAg                                                                                                                   <  2:668477/143‑1 (MQ=255)
                                           tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                                                                            <  2:1685259/150‑1 (MQ=255)
                                              tGAGAACCGAAGTCCATAATGAGCATGCGAGGCATATGAATGTTTTCCGTCATTTACGCTTATTCCGAGGCAAGTTAAACAGATAATATAAATCGCCCGACATGACCTCGGGCGAAGAGAATAAGTAGCCCAGACGGTAGTTCTGGGAct                                                                                                         <  2:183326/150‑1 (MQ=255)
                                               gagaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                                                                        <  2:18553/150‑1 (MQ=255)
                                                  aaCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                                                                     <  2:511171/150‑1 (MQ=255)
                                                  aaCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                                                                     <  2:195195/150‑1 (MQ=255)
                                                    ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAATTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                                   <  2:1077921/150‑1 (MQ=255)
                                                    ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGGAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                                   <  1:396130/150‑1 (MQ=255)
                                                    ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGAGGACTCTTTAg                                                                                                   <  2:156217/150‑1 (MQ=255)
                                                      gAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGCCGGTAGTTCGGGGACTCTTTAGTa                                                                                                 >  1:92011/1‑150 (MQ=255)
                                                       aaGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTaa                                                                                                <  1:1326546/150‑1 (MQ=255)
                                                       aaGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTaa                                                                                                <  1:1319444/150‑1 (MQ=255)
                                                         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                              >  2:74356/1‑150 (MQ=255)
                                                         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                              <  2:1600919/150‑1 (MQ=255)
                                                         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                              <  2:1655503/150‑1 (MQ=255)
                                                          tCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                                                                             <  1:74356/150‑1 (MQ=255)
                                                           ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGt                                                                                            >  2:795667/1‑150 (MQ=255)
                                                            cAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTc                                                                                           >  2:653272/1‑150 (MQ=255)
                                                             aGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCa                                                                                          >  2:1621719/1‑150 (MQ=255)
                                                               aaTGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGTGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcg                                                                                        <  2:896743/150‑1 (MQ=255)
                                                                   aGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                                                                    >  2:573764/1‑150 (MQ=255)
                                                                   aGGATGCGATGCATATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                                                                    <  2:1412782/150‑1 (MQ=255)
                                                                     gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCATGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGATTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                                  <  2:583968/150‑1 (MQ=255)
                                                                     gatgcgatgcTTATGAATGTTTTCCGACATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGCAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                                  <  2:835224/150‑1 (MQ=255)
                                                                        gcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACg                                                                               <  2:1636194/150‑1 (MQ=255)
                                                                         cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTGTTTAGTAATGGTCACGTCGTGAACGt                                                                              >  2:1536967/1‑150 (MQ=255)
                                                                         cgatgcATATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGt                                                                              <  2:351349/150‑1 (MQ=255)
                                                                          gatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTg                                                                             <  1:1621719/150‑1 (MQ=255)
                                                                           atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTgg                                                                            <  2:417941/150‑1 (MQ=255)
                                                                             gcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCACAGACGGTAGTTCTGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCt                                                                          >  1:148295/1‑150 (MQ=255)
                                                                                tATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTc                                                                       >  2:747026/1‑150 (MQ=255)
                                                                                   taaTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                                                                    <  1:499527/149‑1 (MQ=255)
                                                                                   taaTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                                                                    <  2:740750/149‑1 (MQ=255)
                                                                                     aTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                                                  >  1:1042481/1‑150 (MQ=255)
                                                                                      ggTTTTCCGGCATTGACGCTTATTCCGAGGCCAGTGACACAGATAATATAATTCGCCCGACCTGTAGTCGGGCGAAGAGAATCAGGAGCCCAGTCGGTAGTTCTTGTACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAAt                                                                 <  1:254767/149‑1 (MQ=255)
                                                                                            ccGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCATTCAGGAAAGccacg                                                           <  2:1258142/150‑4 (MQ=255)
                                                                                                  gtgtcGCTTATTCCGACGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTGCGGGGACTCTCTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGcg                                                     <  1:137178/146‑2 (MQ=255)
                                                                                                    gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCTGGCGAAGAGAATCAGGAGCCAAGACGGTAGTTCGGTGACTCTTTAGTAATTGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGa                                                   >  1:1043394/1‑150 (MQ=255)
                                                                                                    gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAACAGGAGCCCAGCCGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTGACTGAATGACCGCACCGCTGa                                                   >  1:857536/1‑150 (MQ=255)
                                                                                                     aCGCTTATTCCGAGGCAAGTGAAACAGATAATTTAAATCGCCCGACATGCAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                  <  2:1516255/150‑1 (MQ=255)
                                                                                                     aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                  <  2:1561632/150‑1 (MQ=255)
                                                                                                     aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                  <  1:747026/150‑1 (MQ=255)
                                                                                                     aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGGGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                  <  2:116193/150‑1 (MQ=255)
                                                                                                     aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATCAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                  <  1:671936/150‑1 (MQ=255)
                                                                                                       gCTTAATCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATAc                                                <  1:1136945/150‑1 (MQ=255)
                                                                                                             tCCGATGCAAGTGAATCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTACGGGGACTCTTTAGTAATGGTCACGTCGAGAACGAGGCTTTCCTGAATGCCCGCACCTCTCATAAGTaaaa                                          >  1:59746/1‑150 (MQ=255)
                                                                                                             tCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACaa                                          <  1:603916/150‑1 (MQ=255)
                                                                                                                gAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCAAGACGGTAGTTCGGGGACTCTTTAGTAATGGTAACGTCGTGAACGAGGCTATCCTTAATGCCCGCACCGATGATACGTACAAACt                                       >  1:574423/1‑150 (MQ=255)
                                                                                                                 aGGCAAGTTACACAGATAATATAAATGGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTc                                      <  1:137103/150‑1 (MQ=255)
                                                                                                                 aGGCAAGTGAAACAGATAATATAAATCGCCCTACATGATGTCGGGCGAAGAGCAGCAGGAGCCCAGACTGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGTCCGCACCGCTGATTCGTACTAACTc                                      <  2:291270/150‑1 (MQ=255)
                                                                                                                  gtcAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTTAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                     <  2:428328/148‑1 (MQ=255)
                                                                                                                  ggCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                     >  1:137313/1‑150 (MQ=255)
                                                                                                                  gacAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                     >  2:1201312/3‑150 (MQ=255)
                                                                                                                   gCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCg                                    >  2:1610970/1‑150 (MQ=255)
                                                                                                                       gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                <  1:800204/150‑1 (MQ=255)
                                                                                                                       gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                >  2:366591/1‑150 (MQ=255)
                                                                                                                       gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                >  2:881923/1‑150 (MQ=255)
                                                                                                                        tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                <  1:1486234/150‑1 (MQ=255)
                                                                                                                        tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACTGTAGTTCGTTGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTa                               <  1:1085296/150‑1 (MQ=255)
                                                                                                                           aaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTa                            <  1:1031180/150‑1 (MQ=255)
                                                                                                                             cAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAACCTCCGCTTTAGTACg                          >  1:1025948/1‑150 (MQ=255)
                                                                                                                             cAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                          >  2:1353169/1‑150 (MQ=255)
                                                                                                                             cAGATAATATAAAACGCCCGACATCACGTCGGGCGAAGAGACTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                          <  1:864704/150‑1 (MQ=255)
                                                                                                                                    tataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcg                   >  1:372236/1‑150 (MQ=255)
                                                                                                                                     ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgt                  >  2:1352056/1‑150 (MQ=255)
                                                                                                                                       tcttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCTGACCGGAGTTCGGGGACTCATAAGTAATGCTCAGGTCGTGGACGTCGCGTTCCAGCAAGCACCCACCGCTGTTACGTACAAACGCCGCTTTATGAGGCAGTtggtcc                >  2:331566/4‑149 (MQ=255)
                                                                                                                                       aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtcg                >  2:1488988/1‑150 (MQ=255)
                                                                                                                                        cttCGCCCGACTTAATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGa               >  1:1070609/3‑150 (MQ=255)
                                                                                                                                        aaTCGCCCGACATGAAGTCGGGCGAAGAGATGCTGGAGCCCTGACGGGTGTTCGGGGACTCTTTAGTAATGGTCACGTGGGGCACGTGGGTTGCCGGAATGCCCGCACCGCTGATACGTACCCACTGCGCGGAAGTACGCCGTTCGGCGa               >  2:350907/1‑150 (MQ=255)
                                                                                                                                         aTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTTGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGGTCGTCGAt              >  2:88007/1‑150 (MQ=255)
                                                                                                                                           cGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGGTTTAGTACGCAGTTCGTCGATat            >  2:905463/1‑149 (MQ=255)
                                                                                                                                           cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAAc                                        <  2:289713/122‑1 (MQ=255)
                                                                                                                                           cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAg            >  1:1335380/1‑150 (MQ=255)
                                                                                                                                              ccGACATGAAGGCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTAc         <  1:1421240/150‑1 (MQ=255)
                                                                                                                                                gACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACca       >  1:1472685/1‑150 (MQ=255)
                                                                                                                                                    tGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGc   >  1:18866/1‑150 (MQ=255)
                                                                                                                                                     gAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGcc  <  2:137313/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCC  >  NC_000913/2632437‑2632735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: