Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,632,588 A→T 5.7% intergenic (‑53/+16) guaA ← / ← guaB GMP synthetase (glutamine aminotransferase)/IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,632,5880AT 5.7% 312.0 / 6.0 116intergenic (‑53/+16)guaA/guaBGMP synthetase (glutamine aminotransferase)/IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (50/57);  new base T (3/4);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 120 total aligned reads displayed.

AAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGG  >  NC_000913/2632439‑2632737
                                                                                                                                                     |                                                                                                                                                      
aaaCACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGaa                                                                                                                                                        >  1:1038146/1‑150 (MQ=255)
    acCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCg                                                                                                                                                   >  2:1310458/1‑150 (MQ=255)
     cccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                                                                                  >  2:603916/1‑150 (MQ=255)
     cccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                                                                                  >  2:1599241/1‑150 (MQ=255)
        aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                               <  1:1053797/150‑1 (MQ=255)
        aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                               <  1:320921/150‑1 (MQ=255)
        aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTCTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                               <  1:231735/150‑1 (MQ=255)
          cTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGaa                                                                                                                                             >  2:137103/1‑150 (MQ=255)
           tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatt                                                                                                                                            <  1:1633064/150‑3 (MQ=255)
           tCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatt                                                                                                                                            <  1:540803/150‑3 (MQ=255)
            cacgcaccCGGCGCGCAACCAGGTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAaga                                                                                                                                           <  1:1064337/150‑1 (MQ=255)
               gcacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGaa                                                                                                                                        <  1:170436/150‑1 (MQ=255)
                cacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAt                                                                                                                                       >  2:1085296/1‑150 (MQ=255)
                 acgcGGCGCGCAACCATTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATc                                                                                                                                      <  2:712364/150‑1 (MQ=255)
                  cgcgGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCa                                                                                                                                     >  1:32188/1‑150 (MQ=255)
                    cggcgCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAgg                                                                                                                                   <  1:862496/150‑1 (MQ=255)
                         cgcAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGccc                                                                                                                              <  2:1074952/150‑1 (MQ=255)
                             aCCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGAc                                                                                                                          >  1:723607/1‑150 (MQ=255)
                                    gAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGCCGGTAGtt                                                                                                                   >  1:392810/1‑150 (MQ=255)
                                         tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAg                                                                                                                     >  1:668477/1‑143 (MQ=255)
                                         tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                                                                              <  2:1685259/150‑1 (MQ=255)
                                            tGAGAACCGAAGTCCATAATGAGCATGCGAGGCATATGAATGTTTTCCGTCATTTACGCTTATTCCGAGGCAAGTTAAACAGATAATATAAATCGCCCGACATGACCTCGGGCGAAGAGAATAAGTAGCCCAGACGGTAGTTCTGGGAct                                                                                                           <  2:183326/150‑1 (MQ=255)
                                            tGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGAct                                                                                                           >  1:666540/1‑150 (MQ=255)
                                             gagaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                                                                          <  2:18553/150‑1 (MQ=255)
                                               gaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCtt                                                                                                        >  2:148495/1‑150 (MQ=255)
                                                aaCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                                                                       <  2:511171/150‑1 (MQ=255)
                                                  ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAATTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                                     <  2:1077921/150‑1 (MQ=255)
                                                  ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGGAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                                     <  1:396130/150‑1 (MQ=255)
                                                  ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGAGGACTCTTTAg                                                                                                     <  2:156217/150‑1 (MQ=255)
                                                    gAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGCCGGTAGTTCGGGGACTCTTTAGTa                                                                                                   >  1:92011/1‑150 (MQ=255)
                                                     aaGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTaa                                                                                                  <  1:1326546/150‑1 (MQ=255)
                                                       gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                                >  2:74356/1‑150 (MQ=255)
                                                       gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                                <  2:1600919/150‑1 (MQ=255)
                                                       gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                                <  2:1655503/150‑1 (MQ=255)
                                                        tCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                                                                               <  1:74356/150‑1 (MQ=255)
                                                         ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGt                                                                                              >  2:795667/1‑150 (MQ=255)
                                                          cAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTc                                                                                             >  2:653272/1‑150 (MQ=255)
                                                           aGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCa                                                                                            >  2:1621719/1‑150 (MQ=255)
                                                             aaTGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGTGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcg                                                                                          <  2:896743/150‑1 (MQ=255)
                                                                gaggagGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcg                                                                                       <  1:1460185/150‑1 (MQ=255)
                                                                 aGGATGCGATGCATATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                                                                      <  2:1412782/150‑1 (MQ=255)
                                                                  ggATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAAAATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGTGACTCTTTAGTAATGGTCACGTCGTg                                                                                     <  2:469838/150‑1 (MQ=255)
                                                                   gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCATGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGATTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                                    <  2:583968/150‑1 (MQ=255)
                                                                   gatgcgatgcTTATGAATGTTTTCCGACATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGCAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                                    <  2:835224/150‑1 (MQ=255)
                                                                       cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTGTTTAGTAATGGTCACGTCGTGAACGt                                                                                >  2:1536967/1‑150 (MQ=255)
                                                                        gatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTg                                                                               >  1:1329849/1‑150 (MQ=255)
                                                                        gatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTg                                                                               <  1:1621719/150‑1 (MQ=255)
                                                                         atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTgg                                                                              <  2:417941/150‑1 (MQ=255)
                                                                           gcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCACAGACGGTAGTTCTGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCt                                                                            >  1:148295/1‑150 (MQ=255)
                                                                           gcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGACCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGATCTTGGCt                                                                            >  1:1653795/1‑150 (MQ=255)
                                                                              tATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTc                                                                         >  2:747026/1‑150 (MQ=255)
                                                                                 taaTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                                                                      <  2:740750/149‑1 (MQ=255)
                                                                                 taaTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                                                                      <  1:499527/149‑1 (MQ=255)
                                                                                   aTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                                                    >  1:1042481/1‑150 (MQ=255)
                                                                                    ggTTTTCCGGCATTGACGCTTATTCCGAGGCCAGTGACACAGATAATATAATTCGCCCGACCTGTAGTCGGGCGAAGAGAATCAGGAGCCCAGTCGGTAGTTCTTGTACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAAt                                                                   <  1:254767/149‑1 (MQ=255)
                                                                                                gtgtcGCTTATTCCGACGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTGCGGGGACTCTCTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGcg                                                       <  1:137178/146‑2 (MQ=255)
                                                                                                  gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCTGGCGAAGAGAATCAGGAGCCAAGACGGTAGTTCGGTGACTCTTTAGTAATTGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGa                                                     >  1:1043394/1‑150 (MQ=255)
                                                                                                  gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAACAGGAGCCCAGCCGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTGACTGAATGACCGCACCGCTGa                                                     >  1:857536/1‑150 (MQ=255)
                                                                                                   aCGCTTATTCCGAGGCAAGTGAAACAGATAATTTAAATCGCCCGACATGCAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                    <  2:1516255/150‑1 (MQ=255)
                                                                                                   aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                    <  1:747026/150‑1 (MQ=255)
                                                                                                   aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGGGAACGTGGCTTTCCTGAATGCCCGCACCGCTGAt                                                    <  2:116193/150‑1 (MQ=255)
                                                                                                     gCTTAATCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATAc                                                  <  1:1136945/150‑1 (MQ=255)
                                                                                                        tATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTa                                               <  2:1701688/150‑1 (MQ=255)
                                                                                                          ttCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACa                                             >  2:297602/1‑150 (MQ=255)
                                                                                                           tCCGATGCAAGTGAATCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTACGGGGACTCTTTAGTAATGGTCACGTCGAGAACGAGGCTTTCCTGAATGCCCGCACCTCTCATAAGTaaaa                                            >  1:59746/1‑150 (MQ=255)
                                                                                                           tCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACaa                                            >  1:810762/1‑150 (MQ=255)
                                                                                                           tCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACaa                                            <  1:603916/150‑1 (MQ=255)
                                                                                                              gAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCAAGACGGTAGTTCGGGGACTCTTTAGTAATGGTAACGTCGTGAACGAGGCTATCCTTAATGCCCGCACCGATGATACGTACAAACt                                         >  1:574423/1‑150 (MQ=255)
                                                                                                               aGGCAAGTTACACAGATAATATAAATGGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTc                                        <  1:137103/150‑1 (MQ=255)
                                                                                                               aGGCAAGTGAAACAGATAATATAAATCGCCCTACATGATGTCGGGCGAAGAGCAGCAGGAGCCCAGACTGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGTCCGCACCGCTGATTCGTACTAACTc                                        <  2:291270/150‑1 (MQ=255)
                                                                                                                gtcAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTTAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                       <  2:428328/148‑1 (MQ=255)
                                                                                                                ggCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                       <  2:1303693/150‑1 (MQ=255)
                                                                                                                gacAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                       >  2:1201312/3‑150 (MQ=255)
                                                                                                                 gCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCg                                      >  2:1610970/1‑150 (MQ=255)
                                                                                                                     gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                  >  2:881923/1‑150 (MQ=255)
                                                                                                                     gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                  <  1:800204/150‑1 (MQ=255)
                                                                                                                     gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                  >  2:366591/1‑150 (MQ=255)
                                                                                                                      tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCttt                                  <  1:1486234/150‑1 (MQ=255)
                                                                                                                      tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACTGTAGTTCGTTGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTa                                 <  1:1085296/150‑1 (MQ=255)
                                                                                                                         aaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTa                              <  1:1031180/150‑1 (MQ=255)
                                                                                                                           cAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAACCTCCGCTTTAGTACg                            >  1:1025948/1‑150 (MQ=255)
                                                                                                                           cAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                            >  2:1353169/1‑150 (MQ=255)
                                                                                                                           cAGATAATATAAAACGCCCGACATCACGTCGGGCGAAGAGACTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                            <  1:864704/150‑1 (MQ=255)
                                                                                                                              ataatataatCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCATGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGt                        >  2:655224/1‑150 (MQ=255)
                                                                                                                                 atatAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACATACAAACTCCGCTTTAGTACGCAGttg                      >  1:987841/1‑149 (MQ=255)
                                                                                                                                   ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgt                    >  2:1352056/1‑150 (MQ=255)
                                                                                                                                    taAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACGCTTTAGTAATGGTCACGTCGTGCACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTGTTGTAGGGAGTtcgtg                   >  1:442052/1‑149 (MQ=255)
                                                                                                                                     tcttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCTGACCGGAGTTCGGGGACTCATAAGTAATGCTCAGGTCGTGGACGTCGCGTTCCAGCAAGCACCCACCGCTGTTACGTACAAACGCCGCTTTATGAGGCAGTtggtcc                  >  2:331566/4‑149 (MQ=255)
                                                                                                                                     aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtcg                  >  2:1488988/1‑150 (MQ=255)
                                                                                                                                      cttCGCCCGACTTAATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGa                 >  1:1070609/3‑150 (MQ=255)
                                                                                                                                      aaTCGCCCGACATGAAGTCGGGCGAAGAGATGCTGGAGCCCTGACGGGTGTTCGGGGACTCTTTAGTAATGGTCACGTGGGGCACGTGGGTTGCCGGAATGCCCGCACCGCTGATACGTACCCACTGCGCGGAAGTACGCCGTTCGGCGa                 >  2:350907/1‑150 (MQ=255)
                                                                                                                                       aTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTTGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGGTCGTCGAt                >  2:88007/1‑150 (MQ=255)
                                                                                                                                         cGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGGTTTAGTACGCAGTTCGTCGATat              >  2:905463/1‑149 (MQ=255)
                                                                                                                                         cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAAc                                          <  2:289713/122‑1 (MQ=255)
                                                                                                                                         cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAAc                                          >  1:289713/1‑122 (MQ=255)
                                                                                                                                         cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAg              >  1:1335380/1‑150 (MQ=255)
                                                                                                                                            ccGACATGAAGGCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTAc           <  1:1421240/150‑1 (MQ=255)
                                                                                                                                              gACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACca         >  1:1472685/1‑150 (MQ=255)
                                                                                                                                                  tGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGc     >  1:18866/1‑150 (MQ=255)
                                                                                                                                                    aaGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCg   <  2:593229/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGG  >  NC_000913/2632439‑2632737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: