Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,640,666 A→C 5.5% intergenic (‑91/+20) hisS ← / ← ispG histidyl tRNA synthetase/1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,640,6660AC 5.5% 334.7 / 8.2 109intergenic (‑91/+20)hisS/ispGhistidyl tRNA synthetase/1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (47/56);  new base C (3/3);  total (50/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

Only 100 of 112 total aligned reads displayed.

GCCGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCA  >  NC_000913/2640517‑2640815
                                                                                                                                                     |                                                                                                                                                     
gCCGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGa                                                                                                                                                       >  1:661142/1‑150 (MQ=255)
 ccGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAagagag                                                                                                                                                      >  2:1368535/1‑150 (MQ=255)
  cGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAgg                                                                                                                                                     >  2:1007382/1‑150 (MQ=255)
         ccAGGCAGGTAATCGTTCATGCCTCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTcc                                                                                                                                              <  2:1675932/150‑1 (MQ=255)
          caggcaggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTccc                                                                                                                                             >  2:1171364/1‑150 (MQ=255)
           agggagggAATCGTTCATGCCGCGAATTGCTGGAAGGTGTTTTGCCACGTTTATTCTCGTTCTGAATCTAAAACTGAACCCTCAACGCTTCCCTCACTGTTTCGGGAGCCATGCGGGTGCAATCATACACGGGAAGCGCCTCGCTTcccg                                                                                                                                            <  1:1328150/150‑2 (MQ=255)
           aggcaggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCa                                                                                                                                            <  1:72683/150‑1 (MQ=255)
            ggcaggGAATCGTTCATGCCGAGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAACGGCACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTGCAATCATACACGGGAAGCGCCTCGCTTcccgt                                                                                                                                           <  2:310920/150‑3 (MQ=255)
             gcaggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGCCTCGCTTcccgtg                                                                                                                                          <  1:614378/150‑4 (MQ=255)
               aggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCAc                                                                                                                                        >  2:1477555/1‑150 (MQ=255)
               aggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCAc                                                                                                                                        >  2:160436/1‑150 (MQ=255)
                ggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACg                                                                                                                                       >  2:1383109/1‑150 (MQ=255)
                  tAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTATCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGtt                                                                                                                                     <  1:181858/150‑1 (MQ=255)
                    aTCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACACTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttat                                                                                                                                   >  2:1174977/1‑150 (MQ=255)
                      cGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttatta                                                                                                                                 >  1:428858/1‑150 (MQ=255)
                      cGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCATCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttatta                                                                                                                                 >  1:128502/1‑150 (MQ=255)
                       gTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTtattat                                                                                                                                <  2:1153576/150‑1 (MQ=255)
                           aTGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTAttttt                                                                                                                            <  1:1331184/150‑1 (MQ=255)
                            tGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTAttttta                                                                                                                           >  2:1099196/1‑149 (MQ=255)
                             gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGTGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                          <  2:500446/150‑1 (MQ=255)
                             gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                          <  2:1021930/150‑1 (MQ=255)
                             gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                          >  1:427136/1‑150 (MQ=255)
                             gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTAGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                          <  2:206233/150‑1 (MQ=255)
                              ccGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCACAATTTTTCGGGAGCCATGCGGGTTAAATCATAAACGGGAAGCGAGGCGCTTCCAATCACGTTATTATTTTTaaa                                                                                                                         >  1:1467295/1‑150 (MQ=255)
                               cgcgAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAAc                                                                                                                        <  2:1479329/150‑1 (MQ=255)
                                  taaTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGGCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctg                                                                                                                     <  1:1485367/149‑1 (MQ=255)
                                  gAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctg                                                                                                                     >  1:722240/1‑150 (MQ=255)
                                   aaTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctgc                                                                                                                    <  2:661142/150‑1 (MQ=255)
                                    aTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctgct                                                                                                                   <  1:1477555/150‑1 (MQ=255)
                                      ggCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGAGCTTCCCATCACGTTATTATTTTTCAACCtgatga                                                                                                                 >  2:1233982/1‑150 (MQ=255)
                                         ttGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACg                                                                                                              >  2:375596/1‑150 (MQ=255)
                                         ttGAATGTTTTTTGCCACGTATATTCTCTTTCTGAATATAAAATAGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACTCGGGATGCGAGGCGCTTCCCAGCACGTTATTATTTTTCAACCTGCTGAACg                                                                                                              <  1:238097/150‑1 (MQ=255)
                                           gAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATAAAAAAATTAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTc                                                                                                            <  2:256480/150‑1 (MQ=255)
                                               gTTTTTTGCCATGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATTCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAAtt                                                                                                        <  1:17309/150‑1 (MQ=255)
                                               gTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAAtt                                                                                                        <  1:1430766/150‑1 (MQ=255)
                                               gTTTTTTGCCACGTTTATTCACTTTCAGAAAATAAAAATGAACCCTCAACGCTTCACTCAATGATTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGTCGCTTCCCATCACGTTAATATTTTTCAACCTGCAGAACGTCAAtt                                                                                                        >  1:1271700/1‑150 (MQ=255)
                                                   tttGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGAc                                                                                                    <  2:1361513/150‑1 (MQ=255)
                                                      gCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGAcgcg                                                                                                 >  1:894698/1‑150 (MQ=255)
                                                        cACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCt                                                                                               >  2:1632964/1‑150 (MQ=255)
                                                         aCGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCtt                                                                                              <  2:12197/150‑1 (MQ=255)
                                                          cGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTc                                                                                             >  1:597012/1‑150 (MQ=255)
                                                           gTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCg                                                                                            >  1:1034652/1‑150 (MQ=255)
                                                            tttATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGt                                                                                           >  2:1572071/1‑150 (MQ=255)
                                                             ttATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTACCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTc                                                                                          >  1:845213/1‑150 (MQ=255)
                                                              tATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTcc                                                                                         <  2:904716/150‑1 (MQ=255)
                                                               aTTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCa                                                                                        <  2:560021/150‑1 (MQ=255)
                                                                 tctcTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGc                                                                                      <  1:640156/150‑1 (MQ=255)
                                                                  ctctTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCt                                                                                     >  2:214624/1‑150 (MQ=255)
                                                                          gAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCt                                                                             <  2:472893/150‑1 (MQ=255)
                                                                              ataAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCTCGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCg                                                                         <  1:837917/150‑1 (MQ=255)
                                                                              ataAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCg                                                                         >  2:1268799/1‑150 (MQ=255)
                                                                               taAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGc                                                                        <  2:1584621/150‑1 (MQ=255)
                                                                                aaaaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCa                                                                       >  2:1717896/1‑150 (MQ=255)
                                                                                aaaaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCa                                                                       >  2:529592/1‑150 (MQ=255)
                                                                                 aaaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGATGACTGGCTTTCGCAc                                                                      >  2:786420/1‑150 (MQ=255)
                                                                                  aaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACg                                                                     <  2:567336/150‑1 (MQ=255)
                                                                                  aaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACg                                                                     <  2:1653846/150‑1 (MQ=255)
                                                                                   aaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCTTCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGa                                                                    >  1:1266020/1‑150 (MQ=255)
                                                                                           cTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGTAGCGAGGCGCTTCCCATCGCGGTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGc                                                            >  2:251623/1‑150 (MQ=255)
                                                                                           cTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGc                                                            <  1:167661/150‑1 (MQ=255)
                                                                                           cTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGc                                                            >  2:813877/1‑150 (MQ=255)
                                                                                           cTCAACGCTTCCCTCAATGATTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAAATCGACGCGCTTCTTCCAGCTGCCTGGCTTTCGCACGAATGCGTGc                                                            >  2:835116/1‑150 (MQ=255)
                                                                                            tCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCt                                                           <  1:1465213/150‑1 (MQ=255)
                                                                                             cAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCtt                                                          <  2:931202/150‑1 (MQ=255)
                                                                                               aCGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATAATTTTTCACCCTGCTGAACGTCAATTCGACGCGCTTCGTCCTCCTGACTGGCTTTCGCACGCAAGCGTGCTTcc                                                        >  1:141942/1‑150 (MQ=255)
                                                                                                cGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTAGCACGCATTCGTGcgaaag                                                       <  1:32438/150‑6 (MQ=255)
                                                                                                  cTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGc                                                     <  2:866808/150‑1 (MQ=255)
                                                                                                  cTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGc                                                     <  2:597012/150‑1 (MQ=255)
                                                                                                      ccTAAATTTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGt                                                 <  2:1497450/150‑1 (MQ=255)
                                                                                                           aTGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATc                                            <  2:26524/150‑1 (MQ=255)
                                                                                                            tGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCa                                           <  2:532278/150‑1 (MQ=255)
                                                                                                             gTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCat                                          >  1:79178/1‑150 (MQ=255)
                                                                                                               ttCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCatat                                        <  2:128502/150‑1 (MQ=255)
                                                                                                               ttCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCatat                                        <  1:519137/150‑1 (MQ=255)
                                                                                                                  gggAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCgt                                     <  1:1608650/150‑1 (MQ=255)
                                                                                                                    gAGCCATGCGGGTGCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCgttg                                   <  2:229400/150‑1 (MQ=255)
                                                                                                                     aGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCgttgt                                  <  2:1032067/150‑1 (MQ=255)
                                                                                                                      gCCATGCGGGTTCAATCATACACGGGAAGCTAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGCCGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTAGATCATATCgttgtt                                 >  1:288677/1‑150 (MQ=255)
                                                                                                                       ccATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGttgttg                                <  1:1174977/150‑1 (MQ=255)
                                                                                                                       caaTGCTGGTTCAATCATACACGTGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGttgttg                                <  2:269752/148‑1 (MQ=255)
                                                                                                                        cATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCCTCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTACGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTtgttgt                               >  1:1547174/1‑150 (MQ=255)
                                                                                                                        cATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTtgttgt                               <  1:460995/150‑1 (MQ=255)
                                                                                                                          tGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTcc                             <  1:214624/150‑1 (MQ=255)
                                                                                                                                 tCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCTCGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGt                      >  2:173839/1‑150 (MQ=255)
                                                                                                                                  cAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTc                     >  1:1095783/1‑150 (MQ=255)
                                                                                                                                      cATACACGGGAAGCGAGGCGCTTCCCATCACTTTATTATTTTTCAACCTGCTGAACGTCAATTCGCCGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTg                 <  2:1397626/150‑1 (MQ=255)
                                                                                                                                         acacGGGAAGCGAAGCGCTTCCCATCAAGTTATTATATTTCAACCTGCTGCACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGAGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTgcgc              >  2:1152043/1‑150 (MQ=255)
                                                                                                                                          gatgggAAGCGCCTCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTGTTTGCGCa             >  1:310315/4‑150 (MQ=255)
                                                                                                                                          cacGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGCCGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCa             >  1:1733210/1‑150 (MQ=255)
                                                                                                                                          cacGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCa             >  2:1269805/1‑150 (MQ=255)
                                                                                                                                           atgggAAGCGCCTCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCAc            >  1:788650/3‑150 (MQ=255)
                                                                                                                                           acGGGAAGCGAGGCGCTTCCCATCACGTTTTTTTTTTTCAACGTGCTGAACGTCTATTCGACGCGCTTCGGCCAGCTGACTGGCTTTCGCACGAATGGGTGCTTCCCGGTGGTCGATGATATCGTTGTTGTCGAGAGGGTCTTTGGGCAc            >  2:898478/1‑150 (MQ=255)
                                                                                                                                            tgggAAGCGCCTCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACg           >  1:1699015/2‑150 (MQ=255)
                                                                                                                                            cGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACg           >  1:164030/1‑150 (MQ=255)
                                                                                                                                            cGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACg           <  1:1368535/150‑1 (MQ=255)
                                                                                                                                              ggAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGcc         >  1:619947/1‑150 (MQ=255)
                                                                                                                                              ggAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGcc         >  1:1004058/1‑150 (MQ=255)
                                                                                                                                              ggAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAAATTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTTGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGcc         <  2:1467295/150‑1 (MQ=255)
                                                                                                                                                 aGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATc      <  2:1526630/150‑1 (MQ=255)
                                                                                                                                                     aGGCGCTTCCCAGCACGTTATTATTTTTCTACCTGCTGAACGTCAAATCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGACTGCGTGCTTCCAGCTGGGCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCa  <  1:272209/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCA  >  NC_000913/2640517‑2640815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: