Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,640,668 G→T 5.7% intergenic (‑93/+18) hisS ← / ← ispG histidyl tRNA synthetase/1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,640,6680GT 5.7% 319.0 / 5.4 107intergenic (‑93/+18)hisS/ispGhistidyl tRNA synthetase/1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (45/55);  new base T (4/3);  total (49/58)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCATA  >  NC_000913/2640519‑2640817
                                                                                                                                                     |                                                                                                                                                     
 gTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGgc                                                                                                                                                      <  2:1352417/150‑1 (MQ=255)
       ccAGGCAGGTAATCGTTCATGCCTCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTcc                                                                                                                                                <  2:1675932/150‑1 (MQ=255)
        caggcaggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTccc                                                                                                                                               >  2:1171364/1‑150 (MQ=255)
         agggagggAATCGTTCATGCCGCGAATTGCTGGAAGGTGTTTTGCCACGTTTATTCTCGTTCTGAATCTAAAACTGAACCCTCAACGCTTCCCTCACTGTTTCGGGAGCCATGCGGGTGCAATCATACACGGGAAGCGCCTCGCTTcccg                                                                                                                                              <  1:1328150/150‑2 (MQ=255)
         aggcaggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCa                                                                                                                                              <  1:72683/150‑1 (MQ=255)
          ggcaggGAATCGTTCATGCCGAGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAACGGCACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTGCAATCATACACGGGAAGCGCCTCGCTTcccgt                                                                                                                                             <  2:310920/150‑3 (MQ=255)
           gcaggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGCCTCGCTTcccgtg                                                                                                                                            <  1:614378/150‑4 (MQ=255)
             aggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCAc                                                                                                                                          >  2:1477555/1‑150 (MQ=255)
             aggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCAc                                                                                                                                          >  2:160436/1‑150 (MQ=255)
              ggTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACg                                                                                                                                         >  2:1383109/1‑150 (MQ=255)
               gTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCGTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGt                                                                                                                                        >  1:791531/1‑150 (MQ=255)
                tAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTATCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGtt                                                                                                                                       <  1:181858/150‑1 (MQ=255)
                  aTCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttat                                                                                                                                     <  1:1331389/150‑1 (MQ=255)
                  aTCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACACTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttat                                                                                                                                     >  2:1174977/1‑150 (MQ=255)
                    cGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttatta                                                                                                                                   >  1:428858/1‑150 (MQ=255)
                    cGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCATCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGttatta                                                                                                                                   >  1:128502/1‑150 (MQ=255)
                     gTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTtattat                                                                                                                                  <  2:1153576/150‑1 (MQ=255)
                         aTGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTAttttt                                                                                                                              <  1:1331184/150‑1 (MQ=255)
                         aTGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTAttttt                                                                                                                              <  2:1137608/150‑1 (MQ=255)
                          tGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTAttttta                                                                                                                             >  2:1099196/1‑149 (MQ=255)
                           gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGTGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                            <  2:500446/150‑1 (MQ=255)
                           gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                            <  2:1021930/150‑1 (MQ=255)
                           gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                            >  1:427136/1‑150 (MQ=255)
                           gCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTAGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCa                                                                                                                            <  2:206233/150‑1 (MQ=255)
                            ccGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCACAATTTTTCGGGAGCCATGCGGGTTAAATCATAAACGGGAAGCGAGGCGCTTCCAATCACGTTATTATTTTTaaa                                                                                                                           >  1:1467295/1‑150 (MQ=255)
                                taaTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGGCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctg                                                                                                                       <  1:1485367/149‑1 (MQ=255)
                                gAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctg                                                                                                                       >  1:722240/1‑150 (MQ=255)
                                 aaTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctgc                                                                                                                      <  2:661142/150‑1 (MQ=255)
                                  aTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctgct                                                                                                                     <  1:160436/150‑1 (MQ=255)
                                  aTGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACctgct                                                                                                                     <  1:1477555/150‑1 (MQ=255)
                                       ttGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACg                                                                                                                >  2:375596/1‑150 (MQ=255)
                                       ttGAATGTTTTTTGCCACGTATATTCTCTTTCTGAATATAAAATAGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACTCGGGATGCGAGGCGCTTCCCAGCACGTTATTATTTTTCAACCTGCTGAACg                                                                                                                <  1:238097/150‑1 (MQ=255)
                                         gAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATAAAAAAATTAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTc                                                                                                              <  2:256480/150‑1 (MQ=255)
                                             gTTTTTTGCCATGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATTCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAAtt                                                                                                          <  1:17309/150‑1 (MQ=255)
                                             gTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAAtt                                                                                                          <  1:1430766/150‑1 (MQ=255)
                                             gTTTTTTGCCACGTTTATTCACTTTCAGAAAATAAAAATGAACCCTCAACGCTTCACTCAATGATTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGTCGCTTCCCATCACGTTAATATTTTTCAACCTGCAGAACGTCAAtt                                                                                                          >  1:1271700/1‑150 (MQ=255)
                                                 tttGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGAc                                                                                                      <  2:1361513/150‑1 (MQ=255)
                                                    gCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGAcgcg                                                                                                   >  1:894698/1‑150 (MQ=255)
                                                      cACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCt                                                                                                 >  2:1632964/1‑150 (MQ=255)
                                                       aCGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCtt                                                                                                <  2:12197/150‑1 (MQ=255)
                                                        cGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTc                                                                                               >  1:597012/1‑150 (MQ=255)
                                                         gTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCg                                                                                              >  1:1034652/1‑150 (MQ=255)
                                                          tttATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGt                                                                                             >  2:1572071/1‑150 (MQ=255)
                                                           ttATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTACCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTc                                                                                            >  1:845213/1‑150 (MQ=255)
                                                            tATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTcc                                                                                           <  2:904716/150‑1 (MQ=255)
                                                             aTTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCa                                                                                          <  2:560021/150‑1 (MQ=255)
                                                               tctcTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGc                                                                                        <  1:640156/150‑1 (MQ=255)
                                                                ctctTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCt                                                                                       >  2:214624/1‑150 (MQ=255)
                                                                        gAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCt                                                                               <  2:472893/150‑1 (MQ=255)
                                                                            ataAAAATGAACGCTCAACGCTTCCCGCAATGTTTCGGGAGCCATGCGGGTTCAATCCTACACGGGAAGCGAGGCGCTTCCCATCACGGTAGTATTTTTCACCCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCg                                                                           >  1:235769/1‑150 (MQ=255)
                                                                            ataAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCTCGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCg                                                                           <  1:837917/150‑1 (MQ=255)
                                                                            ataAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCg                                                                           >  2:1268799/1‑150 (MQ=255)
                                                                             taAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGc                                                                          <  2:1584621/150‑1 (MQ=255)
                                                                              aaaaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCa                                                                         >  2:529592/1‑150 (MQ=255)
                                                                              aaaaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCa                                                                         >  2:1717896/1‑150 (MQ=255)
                                                                               aaaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGATGACTGGCTTTCGCAc                                                                        >  2:786420/1‑150 (MQ=255)
                                                                                aaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACg                                                                       <  2:567336/150‑1 (MQ=255)
                                                                                aaaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACg                                                                       <  2:1653846/150‑1 (MQ=255)
                                                                                 aaTGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCTTCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGa                                                                      >  1:1266020/1‑150 (MQ=255)
                                                                                         cTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGTAGCGAGGCGCTTCCCATCGCGGTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGc                                                              >  2:251623/1‑150 (MQ=255)
                                                                                         cTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGc                                                              <  1:167661/150‑1 (MQ=255)
                                                                                         cTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGc                                                              >  2:813877/1‑150 (MQ=255)
                                                                                         cTCAACGCTTCCCTCAATGATTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAAATCGACGCGCTTCTTCCAGCTGCCTGGCTTTCGCACGAATGCGTGc                                                              >  2:835116/1‑150 (MQ=255)
                                                                                          tCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCt                                                             <  1:1465213/150‑1 (MQ=255)
                                                                                           cAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCtt                                                            <  2:931202/150‑1 (MQ=255)
                                                                                             aCGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATAATTTTTCACCCTGCTGAACGTCAATTCGACGCGCTTCGTCCTCCTGACTGGCTTTCGCACGCAAGCGTGCTTcc                                                          >  1:141942/1‑150 (MQ=255)
                                                                                              cGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTAGCACGCATTCGTGcgaaag                                                         <  1:32438/150‑6 (MQ=255)
                                                                                                cTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGc                                                       <  2:866808/150‑1 (MQ=255)
                                                                                                cTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGc                                                       <  2:597012/150‑1 (MQ=255)
                                                                                                    ccTAAATTTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGt                                                   <  2:1497450/150‑1 (MQ=255)
                                                                                                         aTGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATc                                              <  2:26524/150‑1 (MQ=255)
                                                                                                          tGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCa                                             <  2:532278/150‑1 (MQ=255)
                                                                                                           gTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCat                                            >  1:79178/1‑150 (MQ=255)
                                                                                                             ttCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCatat                                          <  2:128502/150‑1 (MQ=255)
                                                                                                             ttCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCatat                                          <  1:519137/150‑1 (MQ=255)
                                                                                                                gggAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCgt                                       <  1:1608650/150‑1 (MQ=255)
                                                                                                                  gAGCCATGCGGGTGCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCgttg                                     <  2:229400/150‑1 (MQ=255)
                                                                                                                   aGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCgttgt                                    <  2:1032067/150‑1 (MQ=255)
                                                                                                                     ccATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGttgttg                                  <  1:1174977/150‑1 (MQ=255)
                                                                                                                     caaTGCTGGTTCAATCATACACGTGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGttgttg                                  <  2:269752/148‑1 (MQ=255)
                                                                                                                      cATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCCTCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTACGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTtgttgt                                 >  1:1547174/1‑150 (MQ=255)
                                                                                                                      cATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTtgttgt                                 <  1:460995/150‑1 (MQ=255)
                                                                                                                        tGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTcc                               <  1:214624/150‑1 (MQ=255)
                                                                                                                               tCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCTCGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGt                        >  2:173839/1‑150 (MQ=255)
                                                                                                                                cAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTc                       >  1:1095783/1‑150 (MQ=255)
                                                                                                                                    cATACACGGGAAGCGAGGCGCTTCCCATCACTTTATTATTTTTCAACCTGCTGAACGTCAATTCGCCGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTg                   <  2:1397626/150‑1 (MQ=255)
                                                                                                                                       acacGGGAAGCGAAGCGCTTCCCATCAAGTTATTATATTTCAACCTGCTGCACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGAGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTgcgc                >  2:1152043/1‑150 (MQ=255)
                                                                                                                                        gatgggAAGCGCCTCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTGTTTGCGCa               >  1:310315/4‑150 (MQ=255)
                                                                                                                                        cacGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGCCGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCa               >  1:1733210/1‑150 (MQ=255)
                                                                                                                                        cacGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCa               <  1:529592/150‑1 (MQ=255)
                                                                                                                                        cacGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCa               >  2:1269805/1‑150 (MQ=255)
                                                                                                                                         atgggAAGCGCCTCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCAc              >  1:788650/3‑150 (MQ=255)
                                                                                                                                         acGGGAAGCGAGGCGCTTCCCATCACGTTTTTTTTTTTCAACGTGCTGAACGTCTATTCGACGCGCTTCGGCCAGCTGACTGGCTTTCGCACGAATGGGTGCTTCCCGGTGGTCGATGATATCGTTGTTGTCGAGAGGGTCTTTGGGCAc              >  2:898478/1‑150 (MQ=255)
                                                                                                                                          tgggAAGCGCCTCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACg             >  1:1699015/2‑150 (MQ=255)
                                                                                                                                            ggAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGcc           >  1:1004058/1‑150 (MQ=255)
                                                                                                                                            ggAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAAATTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTTGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGcc           <  2:1467295/150‑1 (MQ=255)
                                                                                                                                               aGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATc        <  2:1526630/150‑1 (MQ=255)
                                                                                                                                                   aGGCGCTTCCCAGCACGTTATTATTTTTCTACCTGCTGAACGTCAAATCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGACTGCGTGCTTCCAGCTGGGCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCa    <  1:272209/150‑1 (MQ=255)
                                                                                                                                                    ggCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCAt   <  2:52354/150‑1 (MQ=255)
                                                                                                                                                     gcgcTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCATa  <  2:787933/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTTCGCCAGGCAGGTAATCGTTCATGCCGCGAATGGCTTGAATGTTTTTTGCCACGTTTATTCTCTTTCTGAATATAAAAATGAACCCTCAACGCTTCCCTCAATGTTTCGGGAGCCATGCGGGTTCAATCATACACGGGAAGCGAGGCGCTTCCCATCACGTTATTATTTTTCAACCTGCTGAACGTCAATTCGACGCGCTTCGTCCAGCTGACTGGCTTTCGCACGAATGCGTGCTTCCAGCTGGTCGATCATATCGTTGTTGTCCAGACGGTCTTTGCGCACGCCATCTTCATA  >  NC_000913/2640519‑2640817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: