Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,698 A→T 8.0% intergenic (+359/‑157) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,6980AT 8.0% 361.5 / 17.1 137intergenic (+359/‑157)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (65/61);  new base T (5/6);  total (70/67)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01

Only 100 of 141 total aligned reads displayed.

TGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGTATT  >  NC_000913/2653549‑2653847
                                                                                                                                                     |                                                                                                                                                     
tgtTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCa                                                                                                                                                       <  1:1393271/150‑1 (MQ=255)
    cGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCg                                                                                                                                                   >  2:942322/1‑150 (MQ=255)
     ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                                  >  2:623080/1‑150 (MQ=255)
     ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                                  >  1:279417/1‑150 (MQ=255)
        aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAAACGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgtatt                                                                                                                                               >  2:423421/1‑146 (MQ=255)
         ataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttt                                                                                                                                              >  1:1547658/1‑150 (MQ=255)
          taaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGttttt                                                                                                                                             <  2:1042949/150‑1 (MQ=255)
          taaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttttt                                                                                                                                             >  1:563496/1‑150 (MQ=255)
           aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                            >  2:541632/1‑150 (MQ=255)
            aaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAATATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttg                                                                                                                                           <  2:411208/150‑1 (MQ=255)
            aaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttg                                                                                                                                           <  2:484308/150‑1 (MQ=255)
            aaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttg                                                                                                                                           <  1:164432/150‑1 (MQ=255)
             aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                          <  1:1446309/150‑1 (MQ=255)
              ataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgtt                                                                                                                                         <  2:755537/150‑1 (MQ=255)
               taaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgttg                                                                                                                                        <  2:1273221/150‑1 (MQ=255)
                aaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgttga                                                                                                                                       >  1:429961/1‑149 (MQ=255)
                 aCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCt                                                                                                                                      <  1:515725/150‑1 (MQ=255)
                  ccTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATATAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttttt                                                                                                                                     <  2:1484003/150‑5 (MQ=38)
                      gcatattaGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACAAGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTcc                                                                                                                                 <  2:1104351/147‑1 (MQ=255)
                       cTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCg                                                                                                                                >  2:194401/1‑150 (MQ=255)
                             aGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACTGGTAAAAGATATTACTAAAACATGTAAATTCACTTTCCTTTTAAAAACAAAAAACCGCCAAAATCACGCGGTTTATTCATGCTGGTCCGGTTcgc                                                                                                                          >  2:1519602/1‑150 (MQ=255)
                             aGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTcgc                                                                                                                          <  1:1433401/150‑1 (MQ=255)
                             aGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCAGGTTcgc                                                                                                                          >  1:1546420/1‑150 (MQ=255)
                              ttCCGAATTCAGACAAATATAAATAAATCCTGATCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTcgcg                                                                                                                         <  2:1680394/149‑1 (MQ=255)
                               tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                        <  1:639637/150‑1 (MQ=255)
                               tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                        <  2:791275/150‑1 (MQ=255)
                                ccGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGc                                                                                                                       <  1:722840/150‑1 (MQ=255)
                                  gAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCt                                                                                                                     >  1:1725610/1‑150 (MQ=255)
                                   aaTTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCtt                                                                                                                    >  2:994425/1‑150 (MQ=255)
                                    aTTCAGACAAATATAAATAAATCCTGCTCAGAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCttt                                                                                                                   >  1:689293/1‑150 (MQ=255)
                                    aTTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCttt                                                                                                                   >  1:1030753/1‑150 (MQ=255)
                                        aGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTcaac                                                                                                               >  2:295938/1‑147 (MQ=255)
                                        aGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcag                                                                                                               >  2:1363677/1‑150 (MQ=255)
                                         gACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcagc                                                                                                              >  1:1574230/1‑150 (MQ=255)
                                         gACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAAACGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcaga                                                                                                              >  2:370212/1‑149 (MQ=255)
                                           cAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCCTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcagcag                                                                                                            >  2:510241/1‑150 (MQ=255)
                                             aaTATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGt                                                                                                          <  2:1548100/150‑1 (MQ=255)
                                              atatAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                         <  1:398247/150‑1 (MQ=255)
                                                 taaatacaTCCTGCTCAAAATTAAAAATTCTAACCTGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTTAATATCAAATCACCGCCAATAGCAGGCGTTTTTTTGTTGCTGGTCAGGTTCGCGGCCTTTCCAGCAGGTTGTa                                                                                                      <  1:277189/150‑1 (MQ=255)
                                                 taaataaaTCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTa                                                                                                      <  1:613794/150‑1 (MQ=255)
                                                   aataaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTAtt                                                                                                    >  2:1282630/1‑150 (MQ=255)
                                                     taaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAc                                                                                                  <  2:530032/150‑1 (MQ=255)
                                                       aatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCg                                                                                                <  1:1378004/150‑1 (MQ=255)
                                                       aatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACAGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCg                                                                                                >  2:1448571/1‑150 (MQ=255)
                                                          ccTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCgtag                                                                                             >  2:312375/1‑150 (MQ=255)
                                                                 aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                      <  2:245403/150‑1 (MQ=255)
                                                                  aaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTATCCAGCAGGTTGTATTACCGTAGTAATGCaa                                                                                     <  1:889072/150‑1 (MQ=255)
                                                                   aaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAg                                                                                    >  1:819299/1‑150 (MQ=255)
                                                                   aaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCATTTCCAGCAGGTTGTATTACCGTAGTAATGCAAg                                                                                    >  1:1015329/1‑150 (MQ=255)
                                                                    aTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAgc                                                                                   <  2:590343/150‑1 (MQ=255)
                                                                       aaaaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGcgcg                                                                                <  2:1707385/150‑1 (MQ=255)
                                                                                aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCgg                                                                       >  2:1418341/1‑150 (MQ=255)
                                                                                   cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                                    >  1:947551/1‑150 (MQ=255)
                                                                                    ggTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGAc                                                                   >  2:550537/1‑150 (MQ=255)
                                                                                     gTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACa                                                                  <  1:623080/150‑1 (MQ=255)
                                                                                       aaaaGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAt                                                                <  1:422234/150‑1 (MQ=255)
                                                                                       aaaaGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAt                                                                <  1:354121/150‑1 (MQ=255)
                                                                                         aaGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATAc                                                              <  1:541632/150‑1 (MQ=255)
                                                                                          aGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCCGCTGAGAcgcgctt                                                             <  2:866048/150‑7 (MQ=255)
                                                                                             tatTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGc                                                          <  2:165327/150‑1 (MQ=255)
                                                                                              atTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGcc                                                         <  1:245649/150‑1 (MQ=255)
                                                                                              atTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGcc                                                         <  1:1309271/150‑1 (MQ=255)
                                                                                                  cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                     >  2:357566/1‑150 (MQ=255)
                                                                                                   ttAAACATGTAAATTCAGTTTCCTTTAAATAACAAAAAACCTCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCTTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                                    <  1:175348/150‑1 (MQ=255)
                                                                                                    tAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAAc                                                   <  1:1544706/150‑1 (MQ=255)
                                                                                                    tAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAAct                                                  >  2:1002122/1‑150 (MQ=255)
                                                                                                     aaaCATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAAct                                                  <  1:1406223/150‑1 (MQ=255)
                                                                                                     aaaCATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAAct                                                  >  2:946874/1‑150 (MQ=255)
                                                                                                        cATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGATACAATACTCGCCAGTAActctc                                               >  2:103391/1‑150 (MQ=255)
                                                                                                            tAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCtttt                                           <  1:935268/150‑1 (MQ=255)
                                                                                                             aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                          >  1:1218846/1‑150 (MQ=255)
                                                                                                                ttCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTc                                       <  1:1282630/150‑1 (MQ=255)
                                                                                                                    cTTTCATTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACACTACTCGCCAGTAACTCTCTTTTTGTCAAGc                                   >  1:519473/1‑150 (MQ=255)
                                                                                                                        ccTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaaa                               <  2:279417/150‑1 (MQ=255)
                                                                                                                        ccTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaaa                               >  2:1145608/1‑150 (MQ=255)
                                                                                                                         cTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAag                              >  2:1043504/1‑150 (MQ=255)
                                                                                                                         cTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAag                              >  2:1057409/1‑150 (MQ=255)
                                                                                                                           ttAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAagaa                            >  2:734394/1‑149 (MQ=255)
                                                                                                                             aaaaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAagagag                          >  2:606664/1‑150 (MQ=255)
                                                                                                                              agcaacaaaaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt                         >  2:910202/4‑150 (MQ=25)
                                                                                                                              aaaaacaaaaaacCTCCAAAATAAGGCTGTTTTTTGTTGCTGTTCCGGTTAGCGGCCTTTCCAGCAGGTTGTATTACCTTAGTAATGCAAGCGCGTCTAATCGTAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt                         <  1:1416513/150‑1 (MQ=38)
                                                                                                                               aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGtt                        >  2:163731/1‑150 (MQ=255)
                                                                                                                               aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGtt                        <  1:423952/150‑1 (MQ=255)
                                                                                                                               aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCAGGATCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGtt                        >  2:118069/1‑150 (MQ=255)
                                                                                                                                aaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGtta                       >  2:873667/1‑150 (MQ=255)
                                                                                                                                 aacaaaaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttat                      >  1:324332/1‑150 (MQ=37)
                                                                                                                                  acaaaaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatt                     >  1:980865/1‑150 (MQ=37)
                                                                                                                                  acaaaaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatt                     >  1:457948/1‑150 (MQ=37)
                                                                                                                                   caaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta                    >  2:226147/1‑150 (MQ=255)
                                                                                                                                     aaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTattatt                  >  1:1114260/1‑150 (MQ=255)
                                                                                                                                     aaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg                 <  1:1002122/150‑1 (MQ=255)
                                                                                                                                         acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAATCAAAAGAGAGTTATTATTGTTc              >  2:1455714/1‑150 (MQ=255)
                                                                                                                                           cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTg            <  2:1574230/150‑1 (MQ=255)
                                                                                                                                             ccAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGtt          >  1:807831/1‑150 (MQ=255)
                                                                                                                                               aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg        >  2:1349288/1‑150 (MQ=255)
                                                                                                                                                 aaTCAGGCTGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAgtg      <  1:1130527/150‑1 (MQ=255)
                                                                                                                                                  aTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAgtgt     <  2:1143459/150‑1 (MQ=255)
                                                                                                                                                    gaGGCGGTTTTTTGTTGCTTGTCCGTTTCGCGGCCTTTCGAGGAGGTTTTATTACCGTTGTAATGCTATCGCGTCTCAGCAGAGACTATTCTCGCCAGTATCTCTCTTTTTGTGAAGCAATAGAGAGTTATTATTGTTCTGTTAGTGtat   <  1:1519602/149‑1 (MQ=255)
                                                                                                                                                    cAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGtat   <  2:1547658/150‑1 (MQ=255)
                                                                                                                                                     aGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGtatt  >  1:47541/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGTATT  >  NC_000913/2653549‑2653847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: