Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,654,143 T→A 9.4% noncoding (289/304 nt) ryfA → novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,654,1430TA 9.4% 371.8 / 23.7 144noncoding (289/304 nt)ryfAnovel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base T (76/54);  new base A (8/5);  total (84/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-02

Only 100 of 146 total aligned reads displayed.

CCGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTT  >  NC_000913/2653994‑2654290
                                                                                                                                                     |                                                                                                                                                   
ccGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGACTCTCTCCGGCGACAATTTACTGGTGGTTAACAGCCGTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCtt                                                                                                                                                     >  2:669801/1‑150 (MQ=255)
 cGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCTAATGCCGCCCttt                                                                                                                                                    >  2:652881/1‑150 (MQ=255)
    acaATGAAGATTCGGTGCTTATGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATATCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCATCAAGTAAGCCCGAATGCCGCCCTTTggg                                                                                                                                                 <  1:119516/150‑1 (MQ=255)
     caATGAAGATACGGTGCTTTTGTAACGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGggc                                                                                                                                                <  2:919977/150‑1 (MQ=255)
      aaTGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGggcg                                                                                                                                               >  2:114501/1‑150 (MQ=255)
       aTGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGggcgg                                                                                                                                              <  2:47541/150‑1 (MQ=255)
          aaGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           <  1:1370236/150‑1 (MQ=255)
            gATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCatat                                                                                                                                         >  2:1326759/1‑150 (MQ=255)
            gATACGGTGCTTTTGTATAGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCattc                                                                                                                                         <  1:66587/150‑3 (MQ=255)
                cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGTCGACAATTTACTGTTGGTTAACAAACTTCAGAGCAGCAAGTAAGCCAGAATGCCGCCCTTAGGGCGGCATATTTTa                                                                                                                                     >  1:86299/1‑150 (MQ=255)
                cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTa                                                                                                                                     <  2:1122353/150‑1 (MQ=255)
                cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTa                                                                                                                                     <  2:318023/150‑1 (MQ=255)
                 ggTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAACCCCGAATGCCGCCCAAAGGGCGGCattcgggct                                                                                                                                    <  1:422259/150‑8 (MQ=255)
                  gTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGa                                                                                                                                   >  2:1079625/1‑150 (MQ=255)
                   tGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGAt                                                                                                                                  <  1:1213305/150‑1 (MQ=255)
                     cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                >  1:5041/1‑150 (MQ=255)
                           tATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           <  2:918366/133‑1 (MQ=255)
                              cGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCt                                                                                                                       >  1:918863/1‑150 (MQ=255)
                              cGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATACGATTCt                                                                                                                       >  1:1655928/1‑150 (MQ=255)
                               gTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTg                                                                                                                      <  2:253497/150‑1 (MQ=255)
                                tACTTATTGTTTCTGTTGCGCTGTTAACCGAGGTAAATAATAACCGTAGTCTCTCCGGCGACAATTTACTGGTGGTTAACTACCTTCAGAGCAGCAAGTAAGCCCTAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGt                                                                                                                     >  2:848039/1‑150 (MQ=255)
                                 aCTTATTGTTTCTGGTGCGCTGTTAAACGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTATGGGCGGCATATTTTAGATTATCCGATTCTGtt                                                                                                                    <  2:457948/150‑1 (MQ=255)
                                   ttATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTa                                                                                                                  >  2:1346127/1‑150 (MQ=255)
                                    tATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTATTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTaa                                                                                                                 >  1:1325321/1‑150 (MQ=255)
                                    tATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTaa                                                                                                                 >  2:512489/1‑150 (MQ=255)
                                    tATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTaa                                                                                                                 >  2:1523826/1‑150 (MQ=255)
                                      ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                               <  1:303988/150‑1 (MQ=255)
                                        gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  1:376616/1‑150 (MQ=255)
                                        gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  1:1140230/1‑150 (MQ=255)
                                         tttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCa                                                                                                            <  1:933893/150‑1 (MQ=255)
                                         tttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCa                                                                                                            <  1:68794/150‑1 (MQ=255)
                                          ttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCGTTGGGCGGCATATTTTAGATTATACGATTATGTTTTAAGTCAc                                                                                                           >  1:1530819/1‑150 (MQ=255)
                                           tCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGCCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACg                                                                                                          <  2:1425729/150‑1 (MQ=255)
                                              ggTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaa                                                                                                       >  2:307619/1‑150 (MQ=255)
                                              ggTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCGTTGGGCGGCATATTTTAGATTATACGATTATGTTTAAAGTCACCCaa                                                                                                       >  1:1437416/1‑150 (MQ=255)
                                               gTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaa                                                                                                      >  1:927547/1‑150 (MQ=255)
                                                tGCGCTGTTAACCGAGGTAAATAATACCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaaa                                                                                                     <  2:1537344/150‑1 (MQ=255)
                                                  cgcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaaaaa                                                                                                   <  1:349743/150‑1 (MQ=255)
                                                   gcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAac                                                                                                  >  2:784638/1‑150 (MQ=255)
                                                   gcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCGTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTTAAGTCACGCAAAAAac                                                                                                  >  1:747743/1‑150 (MQ=255)
                                                    cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 <  1:1086862/150‑1 (MQ=255)
                                                         aaCCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCAcccc                                                                                            >  2:741429/1‑150 (MQ=255)
                                                             gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCg                                                                                        >  1:1086804/1‑150 (MQ=255)
                                                             gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCg                                                                                        >  1:1354779/1‑150 (MQ=255)
                                                             gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTCGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCAGATTCAGTTTAAAGTCACGCAAAAAACCTCCCCAGCg                                                                                        >  1:1165109/1‑150 (MQ=255)
                                                                  aaaTAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGtt                                                                                   >  2:600556/1‑150 (MQ=255)
                                                                   aataatAACCGGAGTCTCTCTGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTc                                                                                  <  2:1507771/150‑1 (MQ=255)
                                                                   aataatAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTc                                                                                  <  2:1202304/150‑1 (MQ=255)
                                                                      aataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATa                                                                               >  2:437345/1‑150 (MQ=255)
                                                                       ataaCCGGAGTCTCTCCGGCTACAATTTACTGGTGGTTAACAACCTTCAGAGCATCAAGTAAGCCCGAATGCCGCCCTTTGGGCTGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAg                                                                              <  2:1131400/150‑1 (MQ=255)
                                                                       ataaCCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAg                                                                              <  2:225910/150‑1 (MQ=255)
                                                                        taaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGa                                                                             >  1:653209/1‑150 (MQ=255)
                                                                          aCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAAt                                                                           <  1:875450/150‑1 (MQ=255)
                                                                           ccGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACACCCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGGATATTTTAGATTATCCGCTTCTGTTTAAAGTCACGCAAAAAACCACCCCTGCGACGATCATAGAATg                                                                          >  2:79382/1‑150 (MQ=255)
                                                                             ggAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGgc                                                                        >  2:1064412/1‑150 (MQ=255)
                                                                               cgTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCATCGACGTTCATAGAATGgcgc                                                                      <  2:333531/149‑1 (MQ=255)
                                                                                    ctcCGGCGACAATTTACTGGTGGTTAACACCCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAatat                                                                 >  2:1691317/1‑150 (MQ=255)
                                                                                    ctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAatat                                                                 >  1:1128381/1‑150 (MQ=255)
                                                                                               aTTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGtaata                                                      >  1:178202/1‑150 (MQ=255)
                                                                                                tttACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTATAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGtaataa                                                     >  2:966797/1‑150 (MQ=255)
                                                                                                     tggtggTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTgg                                                >  1:1344991/1‑150 (MQ=255)
                                                                                                         ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTaa                                            >  1:149416/1‑150 (MQ=255)
                                                                                                         ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATGGTGTTTAAAGTCCCGCATAAAACCACCCCAGCGACGTTCATAGACTGGCGCAATATGTTCGGTAATAAAGTGGCTaa                                            >  2:93660/1‑150 (MQ=255)
                                                                                                           ttAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAAtt                                          >  1:1443229/1‑150 (MQ=255)
                                                                                                             aacaacCTTCAGAGCAGCAAGCAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTcc                                        <  2:1272318/150‑1 (MQ=255)
                                                                                                              acaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCt                                       >  1:379889/1‑150 (MQ=255)
                                                                                                               aaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCtt                                      <  2:1259322/149‑1 (MQ=255)
                                                                                                                aacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCttt                                     <  1:784638/150‑1 (MQ=255)
                                                                                                                 acCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCtttt                                    <  2:816556/150‑1 (MQ=255)
                                                                                                                  ccTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCAGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCttttt                                   >  2:1681102/1‑150 (MQ=255)
                                                                                                                    ttCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTcc                                 >  1:635995/1‑150 (MQ=255)
                                                                                                                      cAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTcccc                               >  2:1485384/1‑150 (MQ=255)
                                                                                                                        gagCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAATTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtt                             <  1:152531/150‑1 (MQ=255)
                                                                                                                         agcagcAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTATTAAAGTGGCTAATTCCTTTTTCCCCttt                            <  2:376616/150‑1 (MQ=255)
                                                                                                                            agcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGAATATGTTTAAAGTCACGCAAAAAACCACCCCAGCCACGTTCAAAGAATGGCGGAATAAGTTAGGTAATAAAGTGGCTAATTACTTTTTCACCtatttt                         >  1:1708809/1‑150 (MQ=255)
                                                                                                                              caAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCGAATTCCTTTTTCCCCTTTTTTCa                       >  1:612394/1‑150 (MQ=255)
                                                                                                                              caAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCa                       >  1:606176/1‑150 (MQ=255)
                                                                                                                              caAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCa                       >  2:1244141/1‑150 (MQ=255)
                                                                                                                                aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAGCCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     >  1:194290/1‑150 (MQ=255)
                                                                                                                                aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     <  1:1079625/150‑1 (MQ=255)
                                                                                                                                aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     >  2:1042694/1‑150 (MQ=255)
                                                                                                                                 gTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGCTTAACCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATATAATTGAGCAATATTTTCGGTAATTAAGTGGCTAATTACTTTTTCCCCTTTTTTCaaca                    >  2:1538489/1‑147 (MQ=255)
                                                                                                                                 gTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCt                    <  2:1325321/150‑1 (MQ=255)
                                                                                                                                   aaGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAACCCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCTTTTTTCCCCTTTTTTCACCTGa                  >  1:913947/1‑150 (MQ=255)
                                                                                                                                   aaGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGa                  >  1:1704986/1‑150 (MQ=255)
                                                                                                                                    ctaaaataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGAc                 >  2:659649/8‑150 (MQ=255)
                                                                                                                                    aGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGAc                 <  2:1437416/150‑1 (MQ=255)
                                                                                                                                     gCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACa                <  1:1485384/150‑1 (MQ=255)
                                                                                                                                     gCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACa                <  2:1364799/150‑1 (MQ=255)
                                                                                                                                         ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  2:745145/3‑150 (MQ=255)
                                                                                                                                         ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  2:31453/3‑150 (MQ=255)
                                                                                                                                         ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  2:41710/3‑150 (MQ=255)
                                                                                                                                           aagccgccCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTAGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACGTAACAAatata          >  1:45768/3‑149 (MQ=255)
                                                                                                                                           aTGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATc          <  1:921130/150‑1 (MQ=255)
                                                                                                                                             gccgccCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGATGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGa        <  1:1326759/150‑1 (MQ=255)
                                                                                                                                               cgccCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGAtt      <  2:1499027/150‑1 (MQ=255)
                                                                                                                                                   cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  >  1:401545/1‑150 (MQ=255)
                                                                                                                                                   cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  >  1:1265695/1‑150 (MQ=255)
                                                                                                                                                   cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  <  1:1245438/150‑1 (MQ=255)
                                                                                                                                                   cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCAACCCAGCGACGTTCATAGAATTGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  <  1:1147445/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTT  >  NC_000913/2653994‑2654290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: