Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,654,144 T→A 9.4% noncoding (290/304 nt) ryfA → novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,654,1440TA 9.4% 388.1 / 22.1 144noncoding (290/304 nt)ryfAnovel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base T (75/55);  new base A (9/5);  total (84/60)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 145 total aligned reads displayed.

CGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTT  >  NC_000913/2653995‑2654290
                                                                                                                                                     |                                                                                                                                                  
cGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCTAATGCCGCCCttt                                                                                                                                                    >  2:652881/1‑150 (MQ=255)
 gCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTg                                                                                                                                                   >  1:633064/1‑150 (MQ=255)
   acaATGAAGATTCGGTGCTTATGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATATCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCATCAAGTAAGCCCGAATGCCGCCCTTTggg                                                                                                                                                 <  1:119516/150‑1 (MQ=255)
   acaATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTggg                                                                                                                                                 <  2:488309/150‑1 (MQ=255)
    caATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGggc                                                                                                                                                >  2:152531/1‑150 (MQ=255)
    caATGAAGATACGGTGCTTTTGTAACGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGggc                                                                                                                                                <  2:919977/150‑1 (MQ=255)
         aaGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           <  1:1370236/150‑1 (MQ=255)
           gATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCatat                                                                                                                                         >  2:1326759/1‑150 (MQ=255)
           gATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCatat                                                                                                                                         >  2:1502819/1‑150 (MQ=255)
           gATACGGTGCTTTTGTATAGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCattc                                                                                                                                         <  1:66587/150‑3 (MQ=255)
              ccGGTGCTTTTGTATCGTCCTAATTGTATCGGGTGCGCTGTTAACCGCGCTAAATAATAACCGGAGGCGCACCGGCGACAATTTACTGGTGGTTAACAACCTGCAGCGCCGCAAGTAAGCCCGACTGCCGCCCACAGGGCGGCattcggg                                                                                                                                      <  1:926216/149‑6 (MQ=255)
               cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGTCGACAATTTACTGTTGGTTAACAAACTTCAGAGCAGCAAGTAAGCCAGAATGCCGCCCTTAGGGCGGCATATTTTa                                                                                                                                     >  1:86299/1‑150 (MQ=255)
               cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGTGCGGCATATTTTa                                                                                                                                     >  1:1708017/1‑150 (MQ=255)
               cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTa                                                                                                                                     <  2:318023/150‑1 (MQ=255)
                ggTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAACCCCGAATGCCGCCCAAAGGGCGGCattcgggct                                                                                                                                    <  1:422259/150‑8 (MQ=255)
                 gTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGa                                                                                                                                   >  2:1079625/1‑150 (MQ=255)
                  tGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGAt                                                                                                                                  <  1:1213305/150‑1 (MQ=255)
                    cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                >  1:5041/1‑150 (MQ=255)
                    cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                >  2:1141627/1‑150 (MQ=255)
                      tttGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGCAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATc                                                                                                                              >  1:1272318/1‑150 (MQ=255)
                       ttGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATcc                                                                                                                             <  2:1325446/150‑1 (MQ=255)
                          tATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           >  1:918366/1‑133 (MQ=255)
                          tATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           <  2:918366/133‑1 (MQ=255)
                            tCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTc                                                                                                                        <  1:384544/150‑1 (MQ=255)
                             cGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTACGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTATATTATCCGATTCt                                                                                                                       >  2:1371041/1‑150 (MQ=255)
                               tACTTATTGTTTCTGTTGCGCTGTTAACCGAGGTAAATAATAACCGTAGTCTCTCCGGCGACAATTTACTGGTGGTTAACTACCTTCAGAGCAGCAAGTAAGCCCTAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGt                                                                                                                     >  2:848039/1‑150 (MQ=255)
                                aCTTATTGTTTCTGGTGCGCTGTTAAACGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTATGGGCGGCATATTTTAGATTATCCGATTCTGtt                                                                                                                    <  2:457948/150‑1 (MQ=255)
                                   tATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTaa                                                                                                                 >  2:512489/1‑150 (MQ=255)
                                   tATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTaa                                                                                                                 >  2:1523826/1‑150 (MQ=255)
                                     ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                               <  1:303988/150‑1 (MQ=255)
                                     ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                               <  2:31850/150‑1 (MQ=255)
                                       gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  1:376616/1‑150 (MQ=255)
                                       gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  1:1140230/1‑150 (MQ=255)
                                        tttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCa                                                                                                            <  1:933893/150‑1 (MQ=255)
                                        tttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCa                                                                                                            <  1:68794/150‑1 (MQ=255)
                                         ttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCGTTGGGCGGCATATTTTAGATTATACGATTATGTTTTAAGTCAc                                                                                                           >  1:1530819/1‑150 (MQ=255)
                                          tCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACg                                                                                                          <  2:1083469/150‑1 (MQ=255)
                                             ggTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaa                                                                                                       >  2:307619/1‑150 (MQ=255)
                                             ggTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCGTTGGGCGGCATATTTTAGATTATACGATTATGTTTAAAGTCACCCaa                                                                                                       >  1:1437416/1‑150 (MQ=255)
                                               tGCGCTGTTAACCGAGGTAAATAATACCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaaa                                                                                                     <  2:1537344/150‑1 (MQ=255)
                                               tGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTATCGACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCAGATTTTAGATTATCCCAATCTGATTAAAGACCCGCaaaa                                                                                                     >  1:753868/1‑150 (MQ=255)
                                                 cgcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaaaaa                                                                                                   <  1:349743/150‑1 (MQ=255)
                                                  gcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAac                                                                                                  >  2:784638/1‑150 (MQ=255)
                                                  gcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCGTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTTAAGTCACGCAAAAAac                                                                                                  >  1:747743/1‑150 (MQ=255)
                                                   cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 <  1:869025/150‑1 (MQ=255)
                                                   cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 <  1:1086862/150‑1 (MQ=255)
                                                        aaCCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATTTTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCAcccc                                                                                            >  2:615267/1‑150 (MQ=255)
                                                        aaCCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCAcccc                                                                                            >  1:42910/1‑150 (MQ=255)
                                                        aaCCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCAcccc                                                                                            >  2:741429/1‑150 (MQ=255)
                                                            gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCg                                                                                        >  1:1086804/1‑150 (MQ=255)
                                                            gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCg                                                                                        >  1:1354779/1‑150 (MQ=255)
                                                            gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTCGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCAGATTCAGTTTAAAGTCACGCAAAAAACCTCCCCAGCg                                                                                        >  1:1165109/1‑150 (MQ=255)
                                                                  aataatAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTc                                                                                  <  2:1202304/150‑1 (MQ=255)
                                                                      ataaCCGGAGTCTCTCCGGCTACAATTTACTGGTGGTTAACAACCTTCAGAGCATCAAGTAAGCCCGAATGCCGCCCTTTGGGCTGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAg                                                                              <  2:1131400/150‑1 (MQ=255)
                                                                      ataaCCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAg                                                                              <  2:225910/150‑1 (MQ=255)
                                                                       taaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGa                                                                             >  1:653209/1‑150 (MQ=255)
                                                                          ccGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACACCCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGGATATTTTAGATTATCCGCTTCTGTTTAAAGTCACGCAAAAAACCACCCCTGCGACGATCATAGAATg                                                                          >  2:79382/1‑150 (MQ=255)
                                                                                   ctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAatat                                                                 >  1:1128381/1‑150 (MQ=255)
                                                                                              aTTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGtaata                                                      >  1:178202/1‑150 (MQ=255)
                                                                                               tttACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGCATGGCGCAATATGTTCGGtaataa                                                     >  2:314068/1‑150 (MQ=255)
                                                                                               tttACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTATAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGtaataa                                                     >  2:966797/1‑150 (MQ=255)
                                                                                                   cTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCAGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTg                                                 <  1:1635227/150‑1 (MQ=255)
                                                                                                    tggtggTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTgg                                                >  1:1344991/1‑150 (MQ=255)
                                                                                                    tggtggTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTgg                                                >  2:123881/1‑150 (MQ=255)
                                                                                                     ggtggtTACCAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGGATTTTTTAGATTTTCCGCTTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATTGAATGGCGCAATATGTTCGGTTATAAAGTGGc                                               >  2:1453927/1‑150 (MQ=255)
                                                                                                     ggtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCAGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTAATAGAATGGCGCAATATGTTCGGTAATAAAGTGGc                                               >  1:553609/1‑150 (MQ=255)
                                                                                                      gtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCt                                              <  2:719547/150‑1 (MQ=255)
                                                                                                        ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTaa                                            >  1:149416/1‑150 (MQ=255)
                                                                                                        ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATGGTGTTTAAAGTCCCGCATAAAACCACCCCAGCGACGTTCATAGACTGGCGCAATATGTTCGGTAATAAAGTGGCTaa                                            >  2:93660/1‑150 (MQ=255)
                                                                                                          ttAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAAtt                                          >  1:1443229/1‑150 (MQ=255)
                                                                                                            aacaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTcc                                        >  2:447083/1‑150 (MQ=255)
                                                                                                             acaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCt                                       >  1:379889/1‑150 (MQ=255)
                                                                                                              aaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCtt                                      <  2:1259322/149‑1 (MQ=255)
                                                                                                                acCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCtttt                                    <  2:816556/150‑1 (MQ=255)
                                                                                                                 ccTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCAGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCttttt                                   >  2:1681102/1‑150 (MQ=255)
                                                                                                                       gagCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAATTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtt                             <  1:152531/150‑1 (MQ=255)
                                                                                                                        agcagcAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTATTAAAGTGGCTAATTCCTTTTTCCCCttt                            <  2:376616/150‑1 (MQ=255)
                                                                                                                             caAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCGAATTCCTTTTTCCCCTTTTTTCa                       >  1:612394/1‑150 (MQ=255)
                                                                                                                               aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAGCCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     >  1:194290/1‑150 (MQ=255)
                                                                                                                               aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     <  1:1079625/150‑1 (MQ=255)
                                                                                                                               aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     >  2:1042694/1‑150 (MQ=255)
                                                                                                                                gTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCt                    <  2:1325321/150‑1 (MQ=255)
                                                                                                                                  aaGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAACCCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCTTTTTTCCCCTTTTTTCACCTGa                  >  1:913947/1‑150 (MQ=255)
                                                                                                                                  aaGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGa                  >  1:1704986/1‑150 (MQ=255)
                                                                                                                                   aGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGAc                 >  2:1605855/1‑150 (MQ=255)
                                                                                                                                   aGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGAc                 <  2:1437416/150‑1 (MQ=255)
                                                                                                                                    gCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACa                <  2:1364799/150‑1 (MQ=255)
                                                                                                                                    gCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACa                <  1:1485384/150‑1 (MQ=255)
                                                                                                                                        ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  2:41710/3‑150 (MQ=255)
                                                                                                                                        ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  2:745145/3‑150 (MQ=255)
                                                                                                                                          aTGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATc          <  1:921130/150‑1 (MQ=255)
                                                                                                                                            gccgccCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGATGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGa        <  1:1326759/150‑1 (MQ=255)
                                                                                                                                              cgccCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGAtt      <  2:1499027/150‑1 (MQ=255)
                                                                                                                                              cgccCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGAtt      <  1:994223/150‑1 (MQ=255)
                                                                                                                                              cgccCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGAtt      <  2:384722/150‑1 (MQ=255)
                                                                                                                                                cccTTTGTGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTgg    <  2:1266431/150‑1 (MQ=255)
                                                                                                                                                 ccTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGt   <  2:1704986/150‑1 (MQ=255)
                                                                                                                                                  cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  >  1:1265695/1‑150 (MQ=255)
                                                                                                                                                  cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  <  1:1245438/150‑1 (MQ=255)
                                                                                                                                                  cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCAACCCAGCGACGTTCATAGAATTGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  <  1:1147445/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTT  >  NC_000913/2653995‑2654290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: